Information on EC 3.5.1.31 - formylmethionine deformylase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
3.5.1.31
-
RECOMMENDED NAME
GeneOntology No.
formylmethionine deformylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
N-formyl-L-methionine + H2O = formate + L-methionine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
-
Glyoxylate and dicarboxylate metabolism
-
SYSTEMATIC NAME
IUBMB Comments
N-formyl-L-methionine amidohydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
N-formyl-L-methionine deformylase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9032-86-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-formyl-alanine + H2O
formate + alanine
show the reaction diagram
-
at 2-7% of the activity with N-formyl-methionine, depending on tissue
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
-
-
-
?
N-formyl-leucine + H2O
formate + leucine
show the reaction diagram
-
at 0.8-12% of the activity with N-formyl-methionine, depending on tissue
-
-
?
additional information
?
-
-
no activity with N-formyl-L-methionyl-L-Ala
-
-
-
additional information
?
-
-
the enzyme may be important in modulation of neutrophil responses to chemoattractant formylmethionyl peptides or similar compounds
-
-
-
additional information
?
-
-
the enzyme might indirectly be involved in protein biosynthesis
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme may be important in modulation of neutrophil responses to chemoattractant formylmethionyl peptides or similar compounds
-
-
-
additional information
?
-
-
the enzyme might indirectly be involved in protein biosynthesis
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
0.1 mM, 7-10fold stimulation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
2 mM, 90-100% inhibition
dithiothreitol
-
2 mM, 90-100% inhibition
EDTA
-
2 mM, 65-80% inhibition
phenylmethanesulfonyl fluoride
-
2 mM, 10% inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.3
-
N-formyl-methionine
-
enzyme from spleen
3.8
-
N-formyl-methionine
-
-
5
-
N-formyl-methionine
-
enzyme from brain, heart or muscle
6.5
-
N-formyl-methionine
-
enzyme from kidney
12.5
-
N-formyl-methionine
-
enzyme from liver
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
-
additional information
-
-
activity in 0.1 M Tris buffer is 10fold lower than in 0.1 M phosphate buffer, pH 8.5
additional information
-
-
27% more active with phosphate than with Tris-Cl- buffer
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9.5
-
pH 6.5: about 75% of maximal activity, pH 9.5: about 50% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Plasmodium falciparum (isolate 3D7)
Plasmodium falciparum (isolate 3D7)
Plasmodium falciparum (isolate 3D7)