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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
glutaminase, l-glutaminase, phosphate-activated glutaminase, mitochondrial glutaminase, glutaminase 1, kidney-type glutaminase, phosphate activated glutaminase, glutaminase a, glnase, glutaminase c,
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glutamine aminohydrolase
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L-glutamine amidohydrolase
phosphate activated glutaminase
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L-glutamine amidohydrolase
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L-glutamine amidohydrolase
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carboxylic acid amide hydrolysis
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L-glutamine amidohydrolase
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D-Asn + H2O
D-Asp + NH3
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glutaminase A
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?
D-Asn + hydroxylamine
D-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
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?
D-asparagine + H2O
D-aspartate + NH3
44% activity
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?
D-gamma-glutamyl-ethylester + H2O
D-Glu + ethanol
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?
D-gamma-glutamyl-ethylester + hydroxylamine
D-gamma-glutamyl hydroxamate + ethanol
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?
D-gamma-glutamyl-hydrazide + H2O
D-Glu + hydroxyhydrazine
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?
D-gamma-glutamyl-hydrazide + hydroxylamine
D-gamma-glutamyl hydroxamate + hydroxyhydrazine
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?
D-gamma-glutamyl-methylester + H2O
D-Glu + methanol
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?
D-gamma-glutamyl-methylester + hydroxylamine
L-gamma-glutamyl hydroxamate + methanol
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?
D-Gln + H2O
D-Glu + NH3
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glutaminase A
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?
D-Gln + hydroxylamine
D-gamma-glutamyl hydroxamate + NH3
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glutaminase A and B
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?
D-glutamine + H2O
D-glutamate + NH3
D-theanine + H2O
D-glutamate + ethylamine
Gln + H2O
Glu + NH3
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?
L-Asn + H2O
L-Asp + NH3
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glutaminase A
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?
L-Asn + hydroxylamine
L-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
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?
L-asparagine + H2O
L-aspartate + NH3
51% activity
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?
L-gamma-Gln-methyl ester + H2O
L-Gln + methanol
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?
L-gamma-glutamyl-anilide + H2O
L-Glu + aniline
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?
L-gamma-glutamyl-anilide + hydroxylamine
L-gamma-glutamyl hydroxamate + aniline
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?
L-gamma-glutamyl-ethylester + H2O
L-Glu + ethanol
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?
L-gamma-glutamyl-hydrazide + H2O
L-Glu + hydroxyhydrazine
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?
L-gamma-glutamyl-hydrazide + hydroxylamine
L-gamma-glutamyl hydroxamate + hydroxyhydrazine
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?
L-gamma-glutamyl-L-Ala + H2O
?
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?
L-gamma-glutamyl-L-Ala + hydroxylamine
gamma-glutamyl hydroxamate + Ala
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?
L-gamma-glutamyl-L-epsilon-Lys + H2O
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L-gamma-glutamyl-L-epsilon-Lys + hydroxylamine
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L-gamma-glutamyl-L-Glu + H2O
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L-gamma-glutamyl-L-Glu + hydroxylamine
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?
L-gamma-glutamyl-methylester + H2O
L-Glu + methanol
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?
L-gamma-glutamyl-methylester + hydroxylamine
L-gamma-glutamyl hydroxamate + methanol
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L-gamma-glutamyl-p-nitroanilide + H2O
?
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?
L-gamma-glutamylhydrazine + H2O
?
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L-Gln + H2O
L-Glu + NH3
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glutaminase A and B
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L-Gln + hydroxylamine
L-gamma-glutamyl hydroxamate + NH3
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glutaminase A and B
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?
L-glutamine + H2O
L-glutamate + NH3
L-theanine + H2O
L-glutamate + ethylamine
additional information
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D-glutamine + H2O
D-glutamate + NH3
65% activity
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D-glutamine + H2O
D-glutamate + NH3
92% activity
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?
D-theanine + H2O
D-glutamate + ethylamine
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?
D-theanine + H2O
D-glutamate + ethylamine
57% activity
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L-glutamine + H2O
L-glutamate + NH3
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L-glutamine + H2O
L-glutamate + NH3
pH 7.2, 30°C
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L-theanine + H2O
L-glutamate + ethylamine
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L-theanine + H2O
L-glutamate + ethylamine
71% activity
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additional information
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no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide
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additional information
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no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide
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L-glutamine + H2O
L-glutamate + NH3
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p-chloromercuribenzoate
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additional information
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no inhibition by HgCl2; no inhibition by p-chloromercuribenzoate
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additional information
no inhibition by HgCl2; no inhibition by p-chloromercuribenzoate
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6.7
D-gamma-Gln-methylester
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glutaminase B
0.92
D-theanine
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glutaminase B
2.1
L-gamma-Gln-methyl ester
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glutaminase B
200
L-gamma-glutamyl-L-Ala
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glutaminase B
0.71
L-Theanine
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glutaminase B
0.11
L-glutamine
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brenda
IFO 3080
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brenda
IFO 3080
SwissProt
brenda
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A0A8G7K8L4_PSEAI
302
0
33028
TrEMBL
-
A0A8G4P3U4_PSEAI
202
0
22667
TrEMBL
-
A0A8G3MRV7_PSEAI
302
0
33046
TrEMBL
-
A0A8F8RI46_PSEAI
212
0
23548
TrEMBL
-
A0A8G4J1T1_PSEAI
212
0
23595
TrEMBL
-
Q8KIU3_PSEAI
204
0
22796
TrEMBL
-
A0A3S0J114_PSEAI
212
0
23556
TrEMBL
-
A0A2V3FYK8_PSEAI
202
0
22695
TrEMBL
-
A0A8G3JY34_PSEAI
302
0
32998
TrEMBL
-
A0A2R3IM68_PSEAI
302
0
33027
TrEMBL
-
A0A8G7FJS0_PSEAI
302
0
33046
TrEMBL
-
A0A8F9V956_PSEAI
212
0
23581
TrEMBL
-
A0A8G6A552_PSEAI
212
0
23595
TrEMBL
-
A0A8G5QJY0_PSEAI
302
0
32996
TrEMBL
-
A0A8F9JR93_PSEAI
202
0
22627
TrEMBL
-
A0A367MHR1_PSEAI
202
0
22738
TrEMBL
-
A0A0S2SW19_PSEAI
314
0
34518
TrEMBL
-
A0A072ZUS1_PSEAI
212
0
23567
TrEMBL
-
A0A8F9NQ82_PSEAI
212
0
23509
TrEMBL
-
A0A8F9JND9_PSEAI
302
0
33044
TrEMBL
-
A0A8G7FFS9_PSEAI
302
0
32967
TrEMBL
-
A0A8G3A5J2_PSEAI
212
0
23533
TrEMBL
-
A0A2R3IY81_PSEAI
207
0
23057
TrEMBL
-
A0A485EQJ7_PSEAI
541
0
59453
TrEMBL
-
A0A2R3IPB1_PSEAI
204
0
22782
TrEMBL
-
A0A6B1Y4M9_PSEAI
212
0
23582
TrEMBL
-
A0A072ZIV7_PSEAI
302
0
33012
TrEMBL
-
A0A0C6FJT1_PSEAI
212
0
23514
TrEMBL
-
A0A367LYF2_PSEAI
113
0
12506
TrEMBL
-
A0A8G5U604_PSEAI
302
0
33011
TrEMBL
-
A0A5E5QX89_PSEAI
302
0
33038
TrEMBL
-
A0A8G5PK16_PSEAI
299
0
32648
TrEMBL
-
A0A0F6UC64_PSEAI
204
0
22796
TrEMBL
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A0A367LU84_PSEAI
65
0
6711
TrEMBL
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137000
sedimentation equilibrium mehod
35000
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4 * 35000, glutaminase A, SDS-PAGE
67000
gel filtration
67000
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4 * 67000, glutaminase B, SDS-PAGE
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tetramer
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4 * 67000, glutaminase B, SDS-PAGE
tetramer
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4 * 35000, glutaminase A, SDS-PAGE
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by addition of ammonium sulfate
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0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little lose of activity
0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little loss of activity
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Oshima, M.; Yamamoto, T.; Soda, K.
Further characterization of glutaminase isoenzymes from Pseudomonas aeruginosa
Agric. Biol. Chem.
40
2251-2256
1976
Pseudomonas aeruginosa
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brenda
Soda, K.; Oshima, M.; Yamamoto, T.
Purification and properties of isozymes of glutaminase from Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
46
1278-1284
1972
Pseudomonas aeruginosa, Pseudomonas aeruginosa (Q9I387)
brenda
3.5.1.2 glutaminase
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The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 
