Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.25.1 - proteasome endopeptidase complex and Organism(s) Bos taurus

for references in articles please use BRENDA:EC3.4.25.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Bos taurus
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
cleavage of peptide bonds with very broad specificity
Synonyms
proteasome, 26s proteasome, 20s proteasome, 26 s proteasome, multicatalytic proteinase, 20 s proteasome, multicatalytic protease, 26s proteasome complex, prosome, proteasome 20s, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20S proteasome
-
-
26S protease
-
-
-
-
27 kDa prosomal protein
-
-
-
-
30 kDa prosomal protein
-
-
-
-
Component Y8
-
-
-
-
GPRO-28
-
-
-
-
HsBPROS26
-
-
-
-
HSN3
-
-
-
-
ingensin
-
-
-
-
large multicatalytic protease
-
-
-
-
macropain
-
-
-
-
multicatalytic endopeptidase complex
-
-
-
-
Multicatalytic endopeptidase complex C7
-
-
-
-
multicatalytic protease
-
-
-
-
multicatalytic proteinase
-
-
-
-
p27K
-
-
-
-
PROS-27
-
-
-
-
PROS-30
-
-
-
-
PROS-Dm25
-
-
-
-
PROS-Dm28.1
-
-
-
-
PROS-Dm29
-
-
-
-
PROS-Dm35
-
-
-
-
prosome
-
-
-
-
proteasome
-
-
-
-
Proteasome component C13
-
-
-
-
Proteasome component C2
-
-
-
-
Proteasome component C3
-
-
-
-
Proteasome component C5
-
-
-
-
Proteasome component C8
-
-
-
-
Proteasome component C9
-
-
-
-
Proteasome component DD4
-
-
-
-
Proteasome component DD5
-
-
-
-
Proteasome component pts1
-
-
-
-
RING12 protein
-
-
-
-
RN3
-
-
-
-
SCL1 suppressor protein
-
-
-
-
TAS-F22/FAFP98
-
-
-
-
TAS-G64
-
-
-
-
TCPR29
-
-
-
-
tricorn protease
-
-
-
-
tricorn proteinase
-
-
-
-
XC3
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
140879-24-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyloxycarbonyl-D-Ala-Leu-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-dALR-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GAPLG-p-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GGF-4-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-GGF-p-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GGL-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Leu-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GPAFG-4-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-GPAFG-p-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GPAGG-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-GPALG-4-aminobenzoate + H2O
?
show the reaction diagram
benzyloxycarbonyl-GPALG-p-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-LAF-4-aminobenzoate + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-LLE-2-naphthylamide + H2O
?
show the reaction diagram
beta-casein + H2O
?
show the reaction diagram
-
-
-
-
?
dihydrofolate reductase + H2O
?
show the reaction diagram
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dihydrofolate reductase + H2O
?
show the reaction diagram
-
directly hydrolyzed by the 20S complex, without any previous ubiquitination. The degradation is increased under oxidative conditions. The folate metabolism may be impaired by an increased degradation of dihydrofolate reductase, mediated by the 20S proteasome
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,4-dichloroisocoumarin
benzyloxycarbonyl-GPAF aldehyde
-
-
benzyloxycarbonyl-GPFL aldehyde
-
-
benzyloxycarbonyl-Leu-Leu-Leu-CHO
-
reversible inhibitor, modulation by sodium ion
benzyloxycarbonyl-LLF aldehyde
-
-
histone H3
-
activity with benzyloxycarbonyl-LAF-4-aminobenzoate, 87%, benzyloxycarbonyl-dALR-4-aminobenzoate, 43%, or benzyloxycarbonyl-LLE-2-naphthylamide, 81% as substrates
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aflatoxin G1
-
specific stimulator of activity
histone H3
-
slective activation of the branched-chain amino acid preferring activity with benzyloxycarbonyl-GPALG-4-aminobenzoate as substrate, 1000%
-
PA28
-
protein activator
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.29
benzyloxycarbonyl-GGF-4-aminobenzoate
-
enzyme from thymus
0.38 - 3.3
benzyloxycarbonyl-GPAFG-4-aminobenzoate
0.14 - 0.57
benzyloxycarbonyl-GPALG-4-aminobenzoate
0.01054
beta-casein
-
at 37°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
benzyloxycarbonyl-GGF-4-aminobenzoate
-
enzyme from thymus
1.98 - 18.8
benzyloxycarbonyl-GPAFG-4-aminobenzoate
0.03 - 12.9
benzyloxycarbonyl-GPALG-4-aminobenzoate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.0026
benzyloxycarbonyl-GPAF aldehyde
0.00005 - 0.0527
benzyloxycarbonyl-GPFL aldehyde
0.0016 - 0.00597
benzyloxycarbonyl-LLF aldehyde
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PSB5_BOVIN
263
0
28609
Swiss-Prot
Mitochondrion (Reliability: 4)
PSB6_BOVIN
239
0
25542
Swiss-Prot
Mitochondrion (Reliability: 5)
PSB7_BOVIN
277
0
30009
Swiss-Prot
other Location (Reliability: 3)
PSB8_BOVIN
276
0
30268
Swiss-Prot
other Location (Reliability: 2)
PSB9_BOVIN
219
0
23404
Swiss-Prot
other Location (Reliability: 2)
PSB10_BOVIN
273
0
29099
Swiss-Prot
other Location (Reliability: 3)
A0A7T0IG86_BOVIN
140
0
15572
TrEMBL
Mitochondrion (Reliability: 5)
A0A3Q1LS46_BOVIN
329
0
34752
TrEMBL
Mitochondrion (Reliability: 3)
A0A7T0IGA0_BOVIN
140
0
15587
TrEMBL
other Location (Reliability: 5)
Q8SQB5_BOVIN
216
0
22892
TrEMBL
other Location (Reliability: 2)
A0A7T0IG90_BOVIN
140
0
15614
TrEMBL
other Location (Reliability: 5)
PSB3_BOVIN
205
0
22993
Swiss-Prot
-
PSB2_BOVIN
201
0
22896
Swiss-Prot
-
PSB1_BOVIN
241
0
26246
Swiss-Prot
other Location (Reliability: 3)
PSB4_BOVIN
264
0
29031
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
650000
-
gel filtration
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vinitsky, A.; Cardozo, C.; Sepp-Lorenzino, L.; Michaud, C.; Orlowski, M.
Inhibition of the proteolytic activity of the multicatalytic proteinase complex (proteasome) by substrate-related peptidyl aldehydes
J. Biol. Chem.
269
29860-29866
1994
Bos taurus
Manually annotated by BRENDA team
Chu-Ping, M.; Slaughters, C.A.; DeMartino, G.N.
Identification, purification and characterization of a protein activator (PA28) of the 20S proteasome (macropain)
J. Biol. Chem.
267
10515-10523
1992
Bos taurus
Manually annotated by BRENDA team
Orlowski, M.
Selective activation of the 20 S proteasome (multicatalytic proteinase complex) by histone H3
Biochemistry
40
15318-15326
2001
Bos taurus
Manually annotated by BRENDA team
Eleuteri, A.M.; Angeletti, M.
The bovine lung 20S proteasome binding to reversible inhibitors: modulation by sodium ion
FEBS Lett.
547
7-10
2003
Bos taurus
Manually annotated by BRENDA team
Eleuteri, A.M.; Angeletti, M.; Lupidi, G.; Tacconi, R.; Bini, L.; Fioretti, E.
Isolation and characterization of bovine thymus multicatalytic proteinase complex
Protein Expr. Purif.
18
160-168
2000
Bos taurus
Manually annotated by BRENDA team
Amici, M.; Sagratini, D.; Pettinari, A.; Pucciarelli, S.; Angeletti, M.; Eleuteri, A.M.
20S proteasome mediated degradation of DHFR: implications in neurodegenerative disorders
Arch. Biochem. Biophys.
422
168-174
2004
Bos taurus
Manually annotated by BRENDA team
Amici, M.; Cecarini, V.; Pettinari, A.; Bonfili, L.; Angeletti, M.; Barocci, S.; Biagetti, M.; Fioretti, E.; Maria Eleuteri, A.
Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis
Biol. Chem.
388
107-117
2007
Bos taurus
Manually annotated by BRENDA team