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Benzoyl-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin 7-amide + H2O
?
-
about 8.5% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Phe + Arg-4-methylcoumarin 7-amide
-
-
-
-
?
Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
?
-
preferred by HCE1
-
-
?
Chorion + H2O
Swollen chorion + ?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
GSKTPQDPTQQ + H2O
GSKTPQ + DPTQQ
-
location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme
-
-
?
KLMLKAPEPF + H2O
KLMLK + APEPF
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 7.6% of the rate with TVQQSDYLIK
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
Peptidyl 4-methylcoumarin 7-amides + H2O
?
-
i.e. MCA-peptides
-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
-
location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme
-
-
?
PLPVRVEEVV + H2O
PLPVR + VEEVV
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 38% of the rate with TVQQSDYLIK
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
QTPSYPQQ + H2O
QTPS + YPQQ
SAPVVRTSQAV + H2O
NPQVPQ + YPSKPQ
-
synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
?
-
-
-
-
?
Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
succinyl-Ala-Pro-Ala-4-methylcoumaryl-7-amide + H2O
?
-
best substrate for both isoforms of HCE
-
-
?
succinyl-Ile-Ile-Trp-4-methylcoumarin 7-amide + H2O
?
-
about 5.7% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
best substrate of peptidyl 4-methylcoumarin 7-amides
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
?
-
about 40% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O
?
-
preferred by HCE2
-
-
?
t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumarin 7-amide + H2O
?
-
about 9% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
TVQQSDYLIK + H2O
TVQQS + DYLIK
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein
-
-
?
VPFELRYPVPA + H2O
VPFELR + YPVPA
-
specific activity: 0.05 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
ZP2 + H2O
?
-
zebrafish egg envelope glycoprotein, cleavage at Ser47/Asp48 and Arg66/Val67 in the unfertilized egg, at Gln79/Ala80 in the fertilized egg
-
-
?
ZP3 + H2O
?
-
zebrafish egg envelope glycoprotein, cleavage at Gln79/Ala80 in the unfertilized egg, at Gln67/Ala68 in the fertilized egg
-
-
?
additional information
?
-
casein + H2O
?
-
-
-
-
?
chorion + H2O
?
-
moderate digestion by both HCE isoforms
-
-
?
chorion + H2O
?
-
-
-
-
?
chorion + H2O
?
-
concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66
-
-
?
chorion + H2O
?
-
catalyzes choriolytic swelling
-
-
?
chorion + H2O
?
-
embryo-secreted enzyme
-
-
?
chorion + H2O
?
-
specific substrate, the hatching enzyme is secreted from embryos for digesting their protective extracellular coats
-
-
?
chorion + H2O
?
-
of Paralichthys olivaceus, specific substrate
-
-
?
Chorion + H2O
Swollen chorion + ?
-
-
-
-
?
Chorion + H2O
Swollen chorion + ?
-
-
-
?
Chorion + H2O
Swollen chorion + ?
-
-
-
?
Chorion + H2O
Swollen chorion + ?
-
enzyme binds stoichiometrically to chorion (0.012 mg HCE/20 mg chorion) and remains bound to swollen chorion at pH 7.5 and is released at pH 10.2
-
?
Chorion + H2O
Swollen chorion + ?
-
i.e. fish egg envelope, hardened chorion of fertilized eggs, isolated from blastulae
swollen chorion + unique proline-rich polypeptides
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
-
specific activity: 0.98 nmol/min/microgram protein
-
-
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
-
specific activity: 1.07 nmol/min/microgram protein
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 17% of the rate with TVQQSDYLIK
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
-
Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 43.7 nmol/30min/microgram enzyme
-
-
?
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 32% of the rate with TVQQSDYLIK
-
-
?
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
-
Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 81.7 nmol/30min/microgram enzyme
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
-
specific activity: 0.95 nmol/min/microgram protein
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
-
specific activity: 1.4 nmol/min/microgram protein
-
-
?
QTPSYPQQ + H2O
QTPS + YPQQ
-
specific activity: 11.6 nmol/min/microgram protein
-
-
?
QTPSYPQQ + H2O
QTPS + YPQQ
-
specific activity: 13.7 nmol/min/microgram protein
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
-
specific activity: 16.0 nmol/min/microgram protein
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
-
specific activity: 23.2 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
-
specific activity: 0.35 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
-
specific activity: 0.66 nmol/min/microgram protein
-
-
?
additional information
?
-
-
isoform ZHE1 cleaves the peptide bonds at the C-terminal side of Arg, Tyr, Asn, Trp, Ala, Asp, Phe and Gly
-
-
?
additional information
?
-
-
cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FHCE efficiently cleaves the Pro-X-Y repeat regions into tripeptides to dodecapeptides to swell the envelope
-
-
?
additional information
?
-
-
unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE
-
-
?
additional information
?
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no substrates are chorion of unfertilized eggs
-
-
?
additional information
?
-
-
exhibits choriolytic and proteolytic activity
-
-
?
additional information
?
-
-
N-tert-butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide, succinyl-Gly-Pro 4-methylcoumarin 7-amide or Leu 4-methylcoumarin 7-amide
-
-
?
additional information
?
-
-
swollen chorion
-
-
?
additional information
?
-
-
cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. HCE cleaves the N-terminal region of ZI-1,2 and ZI-3, mainly the Pro-Xaa-Yaa repeat sequence of ZI-1,2 into hexapeptides, but not the site within a zona pellucida (ZP) domain that is considered to be the core structure of the egg envelope. The cleavage of the N-terminal regions results in swelling and softening of the envelope
-
-
?
additional information
?
-
-
the enzyme is a trypsin-type serine protease
-
-
?
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Yasumasu, S.; Katow, S.; Hamazaki, T.S.; Iuchi, I.; Yamagami, K.
Two constituent proteases of a teleostean hatching enzyme: concurrent syntheses and packaging in the same secretory granules in discrete arrangement
Dev. Biol.
149
349-356
1992
Oryzias latipes
brenda
Yasumasu, S.; Yamada, K.; Akasaka, K.; Mitsunaga, K.; Iuchi, I.; Shimada, H.; Yamagami, K.
Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development
Dev. Biol.
153
250-258
1992
Oryzias latipes (P31581), Oryzias latipes
brenda
Yasumasu, S.; Iuchi, I.; Yamagami, K.
Purification and partial characterization of high choriolytic enzyme (HCE), a component of the hatching enzyme of the teleost, Oryzias latipes
J. Biochem.
105
204-211
1989
Oryzias latipes
brenda
Yasumasu, S.; Katow, S.; Umino, Y.; Iuchi, I.; Yamagami, K.
A unique proteolytic action of HCE, a constituent protease of a fish hatching enzyme: tight binding to its natural substrate, egg envelope
Biochem. Biophys. Res. Commun.
162
58-63
1989
Oryzias latipes
brenda
Lee, K.S.; Yasumasu, S.; Nomura, K.; Iuchi, I.
HCE, a constituent of the hatching enzymes of Oryzias latipes embryos, releases unique proline-rich polypeptides from its natural substrate, the hardened chorion
FEBS Lett.
339
281-284
1994
Oryzias latipes
brenda
Kudo, N.; Yasumasu, S.; Iuchi, I.; Tanokura, M.
Crystallization and preliminary X-ray analysis of HCE-1, a hatching enzyme of medaka fish, Oryzias latipes
Acta crystallogr. Sect. D
60
725-726
2004
Oryzias latipes
brenda
Hiroi, J.; Maruyama, K.; Kawazu, K.; Kaneko, T.; Ohtani-Kaneko, R.; Yasumasu, S.
Structure and developmental expression of hatching enzyme genes of the Japanese eel Anguilla japonica: an aspect of the evolution of fish hatching enzyme gene
Dev. Genes Evol.
214
176-184
2004
Anguilla japonica
brenda
Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Hiroi, J.; Yoshizaki, N.; Nagata, K.; Tanokura, M.; Iuchi, I.
Purification and gene cloning of Fundulus heteroclitus hatching enzyme. A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts, Fundulus heteroclitus and medaka Oryzias latipes
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272
4315-4326
2005
Fundulus heteroclitus
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Yasumasu, S.; Yamagami, K.
Choriolysin H
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
625-627
2004
Oryzias latipes
-
brenda
Olivotto, I.; Yasumasu, S.; Gioacchini, G.; Maradonna, F.; Cionna, C.; Carnevali, O.
Cloning and expression of high choriolytic enzyme, a component of the hatching enzyme system, during embryonic development of the marine ornamental fish Chrysiptera parasema
Mar. Biol.
145
1235-1241
2004
Chrysiptera parasema (Q8AY25)
-
brenda
Kawaguchi, M.; Yasumasu, S.; Hiroi, J.; Naruse, K.; Inoue, M.; Iuchi, I.
Evolution of teleostean hatching enzyme genes and their paralogous genes
Dev. Genes Evol.
216
769-784
2006
Oryzias latipes
brenda
Shi, Z.; Fan, T.; Cong, R.; Wang, X.; Sun, W.; Yang, L.
Purification and characterization of hatching enzyme from flounder Paralichthys olivaceus
Fish Physiol. Biochem.
32
35-42
2006
Paralichthys olivaceus
brenda
Kawaguchi, M.; Nakagawa, M.; Noda, T.; Yoshizaki, N.; Hiroi, J.; Nishida, M.; Iuchi, I.; Yasumasu, S.
Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii - environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene
FEBS J.
275
2884-2898
2008
Sebastes schlegelii (B0I2Z7), Sebastes schlegelii (B0I2Z8), Sebastes schlegelii
brenda
Sano, K.; Inohaya, K.; Kawaguchi, M.; Yoshizaki, N.; Iuchi, I.; Yasumasu, S.
Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion
FEBS J.
275
5934-5946
2008
Danio rerio
brenda
Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Sano, K.; Iuchi, I.; Nishida, M.
Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes
FEBS J.
277
4973-4987
2010
Fundulus heteroclitus
brenda
Yasumasu, S.; Kawaguchi, M.; Ouchi, S.; Sano, K.; Murata, K.; Sugiyama, H.; Akema, T.; Iuchi, I.
Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes
J. Biochem.
148
439-448
2010
Oryzias latipes
brenda
Kawaguchi, M.; Tomita, K.; Sano, K.; Kaneko, T.
Molecular events in adaptive evolution of the hatching strategy of ovoviviparous fishes
J. Exp. Zool. B
324
41-50
2015
Xiphophorus maculatus
brenda
Kawaguchi, M.; Nakano, Y.; Kawahara-Miki, R.; Inokuchi, M.; Yorifuji, M.; Okubo, R.; Nagasawa, T.; Hiroi, J.; Kono, T.; Kaneko, T.
An evolutionary insight into the hatching strategies of pipefish and seahorse embryos
J. Exp. Zool. B Mol. Dev. Evol.
326
125-135
2016
Hippocampus abdominalis
brenda
Sano, K.; Yokoyama, R.; Kitano, T.; Takegaki, T.; Kitazawa, N.; Kaneko, T.; Nishino, Y.; Yasumasu, S.; Kawaguchi, M.
Male parental assistance in embryo hatching of barred-chin blenny Rhabdoblennius nitidus
J. Exp. Zool. B Mol. Dev. Evol.
332
81-91
2019
Rhabdoblennius nitidus
brenda