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Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
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hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
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-
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
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specific activity: 8.66 nmol/min/microgram protein
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-
?
EVQPPDSPLSI + H2O
EVQPPD + SPLSI
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location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme
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-
?
GSKGSDETFH + H2O
GSKGS + DETFH
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-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
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location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme
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-
?
PKLFQDGKPSN + H2O
PKLFQ + DGKPSN
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location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme
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-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
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specific activity: 0.56 nmol/min/microgram protein
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?
RPTFGRPGIT + H2O
RPTFG + RPGIT
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-
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?
RPTIGRPGFT + H2O
RPTIG + RPGFT
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-
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?
RPTLGEPTHT + H2O
RPTLG + EPTHT
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-
-
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?
RPTLGGPKGS + H2O
RPTLG + GPKGS
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?
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
?
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-
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?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
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best substrate of peptidyl 4-methylcoumarin 7-amides
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?
SVPVVRTSQAA + H2O
SVPVVR + TSQAA
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specific activity: 19.5 nmol/min/microgram protein
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-
?
VPFELRYPVPA + H2O
VPFELR + YPVPA
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specific activity: 10.36 nmol/min/microgram protein
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?
VPFEQRYPVPA + H2O
VPFEQR + YPVPA
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location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme
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-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
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specific activity: 2.71 nmol/min/microgram protein
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additional information
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casein + H2O
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chorion + H2O
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synergetic digestion by HCE and LCE
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?
chorion + H2O
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?
Swollen chorion + H2O
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?
Swollen chorion + H2O
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i.e. partially digested chorion
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?
Swollen chorion + H2O
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i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope
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?
additional information
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egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions
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additional information
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cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FLCE cleaves the inside of the zona pellucida domain, the core structure of egg envelope subunit protein, to completely digest the FHCE swollen envelope
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additional information
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unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE
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additional information
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additional information
?
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poor substrates are succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide, succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide, no substrates are intact chorion or Gly-Pro 4-methylcoumarin 7-amide
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?
additional information
?
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cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. LCE cleaves the middle of the zona pellucida (ZP) domain of ZI-1,2, in addition to the upstream of the trefoil domain of ZI-1,2 and the ZP domain of ZI-3. This middle site is in the intervening sequence connecting two subdomains of the ZP domain. Cleaving this site results in the solubilization of the swollen egg envelope by the disruption of the filamentous structure that is formed by the non-covalent polymerization of ZP domains
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?
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Yasumasu, S.; Iuchi, I.; Yamagami, K.
Isolation and some properties of low choriolytic enzyme (LCE), a component of the hatching enzyme of the teleost, Oryzias latipes
J. Biochem.
105
212-218
1989
Oryzias latipes
brenda
Yasumasu, S.; Katow, S.; Hamazaki, T.S.; Iuchi, I.; Yamagami, K.
Two constituent proteases of a teleostean hatching enzyme: concurrent syntheses and packaging in the same secretory granules in discrete arrangement
Dev. Biol.
149
349-356
1992
Oryzias latipes
brenda
Yasumasu, S.; Yamada, K.; Akasaka, K.; Mitsunaga, K.; Iuchi, I.; Shimada, H.; Yamagami, K.
Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development
Dev. Biol.
153
250-258
1992
Oryzias latipes
brenda
Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Hiroi, J.; Yoshizaki, N.; Nagata, K.; Tanokura, M.; Iuchi, I.
Purification and gene cloning of Fundulus heteroclitus hatching enzyme. A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts, Fundulus heteroclitus and medaka Oryzias latipes
FEBS J.
272
4315-4326
2005
Fundulus heteroclitus
brenda
Donato, D.M.; Hiramatsu, N.; Arey, K.M.; Hiramatsu, K.; Kennedy, A.M.; Morton, C.L.; Hara, A.; Sullivan, C.V.
Atresia in temperate basses: cloning of hatching enzyme (choriolysin) homologues from atretic ovaries
Fish Physiol. Biochem.
28
329-330
2004
Morone saxatilis
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brenda
Yasumasu, S.; Yamagami, K.
Choriolysin L
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
623-625
2004
Oryzias latipes
-
brenda
Kawaguchi, M.; Yasumasu, S.; Hiroi, J.; Naruse, K.; Inoue, M.; Iuchi, I.
Evolution of teleostean hatching enzyme genes and their paralogous genes
Dev. Genes Evol.
216
769-784
2006
Oryzias latipes
brenda
Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Sano, K.; Iuchi, I.; Nishida, M.
Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes
FEBS J.
277
4973-4987
2010
Fundulus heteroclitus
brenda
Sano, K.; Kawaguchi, M.; Yoshikawa, M.; Kaneko, T.; Tanaka, T.; Iuchi, I.; Yasumasu, S.
Hatching enzyme of Japanese eel Anguilla japonica and the possible evolution of the egg envelope digestion mechanism
FEBS J.
278
3711-3723
2011
Anguilla japonica
brenda
Yasumasu, S.; Kawaguchi, M.; Ouchi, S.; Sano, K.; Murata, K.; Sugiyama, H.; Akema, T.; Iuchi, I.
Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes
J. Biochem.
148
439-448
2010
Oryzias latipes
brenda
Kawaguchi, M.; Nakano, Y.; Kawahara-Miki, R.; Inokuchi, M.; Yorifuji, M.; Okubo, R.; Nagasawa, T.; Hiroi, J.; Kono, T.; Kaneko, T.
An evolutionary insight into the hatching strategies of pipefish and seahorse embryos
J. Exp. Zool. B Mol. Dev. Evol.
326
125-135
2016
no activity in Hippocampus abdominalis
brenda
Sano, K.; Yokoyama, R.; Kitano, T.; Takegaki, T.; Kitazawa, N.; Kaneko, T.; Nishino, Y.; Yasumasu, S.; Kawaguchi, M.
Male parental assistance in embryo hatching of barred-chin blenny Rhabdoblennius nitidus
J. Exp. Zool. B Mol. Dev. Evol.
332
81-91
2019
Rhabdoblennius nitidus
brenda