Information on EC 3.4.24.21 - astacin

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The expected taxonomic range for this enzyme is: Bilateria

EC NUMBER
COMMENTARY hide
3.4.24.21
-
RECOMMENDED NAME
GeneOntology No.
astacin
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of arylamide bond
-
-
-
-
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
143179-21-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Ace-mtp-2
UniProt
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
crayfish
-
-
Manually annotated by BRENDA team
gene dpy-31
UniProt
Manually annotated by BRENDA team
crab
-
-
Manually annotated by BRENDA team
gene dpy-31
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Onchocerca volvolus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
an ovine gastrointestinal nematode, gene dpy-31
UniProt
Manually annotated by BRENDA team
an ovine gastrointestinal nematode, gene dpy-31
UniProt
Manually annotated by BRENDA team
diverse genes
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
the enzyme Xhe2 expression depends entirely on transcription factor Pax3 function. Pax3 can induce premature hatching through the upregulation of several proteolytic enzymes including enzyme Xhe2
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ala)n 4-nitroanilide + H2O
?
show the reaction diagram
-
n: 1,2,3
-
-
-
2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser
-
-
2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser
-
-
2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
fluorogenic bradykinin analog substrate, cleavage sites: Gly-Ala and Ala-Ser
major products
-
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
fluorogenic bradykinin analog substrate, cleavage site: Glu-Ser
-
-
2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Lys + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
fluorogenic bradykinin analog substrate, cleavage site: Lys-Ser
-
-
2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
-
best of fluorogenic bradykinin analog substrates, cleavage site: Phe-Ser
-
-
Abz-YEFDGKSMQGDDPNK-2,4-dinitrophenyl + H2O
Abz-YEFDGKSMQG + DDPNK-2,4-dinitrophenyl
show the reaction diagram
-
Dentin sialophosphoprotein (Dpp) cleavage site, designated Dspp-FRET
-
-
?
Acetyl-Ala-Ala-Ala methylester + H2O
?
show the reaction diagram
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg
show the reaction diagram
-
chromogenic bradykinin analog
-
-
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg
show the reaction diagram
-
chromogenic bradykinin analog
-
-
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg
show the reaction diagram
-
chromogenic bradykinin analog
-
-
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg
show the reaction diagram
-
chromogenic bradykinin analog
-
-
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
i.e. nitrobradykinin, chromogenic bradykinin analog
-
-
azocasein + H2O
?
show the reaction diagram
Bradykinin + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
-
bradykinin 1-7 RPPGFSP + H2O
?
show the reaction diagram
partial digestion
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
-
-
-
?
Collagen IV + H2O
?
show the reaction diagram
-
-
-
-
?
Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val + H2O
Dansyl-Ala-Ala-Arg + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-Arg-Ala-Pro-Leu + H2O
Dansyl-Arg + Ala-Pro-Leu
show the reaction diagram
Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly + H2O
Dansyl-Gly-Arg-Arg + Ala-Pro-Leu-Gly
show the reaction diagram
Dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly + H2O
Dansyl-Gly-Arg-Arg + Ala-Ser-Leu-Gly
show the reaction diagram
Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-Gly-Gly-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val + H2O
Dansyl-Gly-Lys-Arg + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-Gly-Lys-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val + H2O
Dansyl-Gly-Lys-Asn + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val + H2O
Dansyl-Gly-Lys-Tyr + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val + H2O
Dansyl-Gly-Pro-Arg + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-His-His-Leu-Lys-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly + H2O
Dansyl-Leu-Arg-Arg + Ala-Pro-Leu-Gly
show the reaction diagram
Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O
Dansyl-Leu-Arg-Arg + Ala-Ser-Leu-Gly
show the reaction diagram
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu + H2O
Dansyl-Leu-Lys-Arg + Ala-Pro-Leu
show the reaction diagram
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val + H2O
Dansyl-Leu-Lys-Arg + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-Leu-Lys-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Leu-Lys-Arg-Leu-Pro-Trp-Val + H2O
Dansyl-Leu-Lys-Arg + Leu-Pro-Trp-Val
show the reaction diagram
Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val + H2O
Dansyl-Leu-Lys-Asn + Ala-Pro-Leu-Val
show the reaction diagram
Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val + H2O
Dansyl-Leu-Lys-Lys + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val + H2O
Dansyl-Leu-Lys-Tyr + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Lys-Arg-Ala-Pro-Leu + H2O
Dansyl-Lys-Arg + Ala-Pro-Leu
show the reaction diagram
Dansyl-Lys-Glu-Thr-Tyr-Ser-Lys + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
Dansyl-Lys-Glu-Thr-Tyr-Ser-Phe + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
Dansyl-Pro-Gln-Gly-Ile-Ala-Gly-(D)-Arg + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-Pro-Lys-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val + H2O
Dansyl-Val-Lys-Arg + Ala-Pro-Trp-Val
show the reaction diagram
Denatured chains of alpha-tubulin + H2O
Hydrolyzed alpha-tubulin chains
show the reaction diagram
Denatured chains of beta-tubulin + H2O
Hydrolyzed beta-tubulin chains
show the reaction diagram
Denatured tubulin + H2O
Hydrolyzed tubulin
show the reaction diagram
-
cleavage pattern
-
-
Des-Arg-bradykinin PPGFSPFR + H2O
?
show the reaction diagram
complete digestion
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
gelatine + H2O
?
show the reaction diagram
-
-
-
-
?
laminin I + H2O
?
show the reaction diagram
-
-
-
-
?
laminin I/nidogen I complex + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-bradykinin KRPPGFSPFR + H2O
?
show the reaction diagram
complete digestion
-
?
PRPKPQQFFGLM-NH2 + H2O
?
show the reaction diagram
complete digestion
-
?
SQT-3 + H2O
?
show the reaction diagram
Suc-Ala-Ala-Ala-4-nitroanilide + H2O
Suc-Ala-Ala-Ala + 4-nitroaniline
show the reaction diagram
Succinyl-(Ala)n 4-nitroanilide + H2O
?
show the reaction diagram
-
n: 2,3,5
-
-
-
Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
Succinyl-Ala-Ala-Ala + p-nitroaniline
show the reaction diagram
Succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Succinyl-Ala-Ala-Pro 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Succinyl-Ala-Ala-Val 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Succinyl-Ala-Pro-Ala 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Succinyl-Pro-Ala-Ala 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
Type I collagen + H2O
?
show the reaction diagram
-
from calf skin
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Collagen + H2O
?
show the reaction diagram
Q9U918
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Q9U918
-
-
-
?
additional information
?
-
Q9U918
optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation of apoenzyme, can replace Zn2+ as catalytic ion yielding 140% of the activity of the zinc-containing enzyme, pentavalent metal coordination
Cu2+
-
activation of apoenzyme, can replace Zn2+ as catalytic ion yielding 37% of the activity of the zinc-containing enzyme, pentavalent metal coordination
K+
secretion of nephrosin can be stimulated by high concentrations of extracellular potassium
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-o-phenanthroline
-
1 mM, isoform LAST, 10% residual activity, isoform LAST_MAM, 12% residual activity
1,10-phenanthroline
1,7-phenanthroline
2,2'-dipyridyl
-
r
3-[3-(1-aminoethenyl)-1,2,4-oxadiazol-5-yl]-6-cyclohexyl-N-hydroxyhexanamide
4-Mercaptoaniline
-
weak
6-cyclohexyl-3-[3-(2,6-dioxo-1,2,3,6-tetrahydropyrimidin-4-yl)-1,2,4-oxadiazol-5-yl]-N-hydroxyhexanamide
6-cyclohexyl-3-[3-[4-(dihydroxymethyl)pyridin-2-yl]-1,2,4-oxadiazol-5-yl]-N-hydroxyhexanamide
6-cyclohexyl-N-hydroxy-3-(3-[1-[(methylsulfonyl)amino]ethenyl]-1,2,4-oxadiazol-5-yl)hexanamide
8-Hydroxyquinoline-5-sulfonic acid
-
r
9-Fluorenylmethoxycarbonyl-Pro-Lys-Phe-PSI(PO2HCH2)-Ala-Pro-Leu-Val
-
-
9-fluorenylmethyloxycarbonyl-KFPCAPLV-OH
-
-
9-fluorenylmethyloxycarbonyl-PKFPCAPLV-OH
-
-
9fluorenylmethyloxycarbonyl-F-PSI[PO2CH2]-APLV-OH
-
-
9fluorenylmethyloxycarbonyl-FPCAPLVOH
-
-
9fluorenylmethyloxycarbonyl-KF-PSI[PO2CH2]-APLV-OH
-
-
9fluorenylmethyloxycarbonyl-PKF-PSI[PO2CH2]-APLV-OH
-
-
Acetyl-Arg-Pro-Gly-Tyr-hydroxamate
-
-
actinonin
alpha-2-Macroglobulin
-
1 mM, isoform LAST, 1,1% residual activity, isoform LAST_MAM, 42% resiudal activity
-
alpha2-Macroglobulin
-
bovine or Astacus astacus hemolymph, ir
-
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
nitrobradykinin as substrate
Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg
-
nitrobradykinin as substrate
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
nitrobradykinin as substrate
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
nitrobradykinin as substrate
benzyloxycarbonyl-PKF-PSI[PO2CH2]-AGP-O-methyl
-
-
benzyloxycarbonyl-PKF-PSI[PO2CH2]-AP-O-methyl
-
-
benzyloxycarbonyl-PKF-PSI[PO2CH2]-APL-O-methyl
-
-
benzyloxycarbonyl-PKF-PSI[PO2CH2]-GPL-O-methyl
-
-
benzyloxycarbonyl-PKK-PSI[PO2CH2]-APLV-O-methyl
-
-
benzyloxycarbonyl-Pro-Lys-Phe-PSI[PO2CH2]Ala-Pro-O-methyl
-
-
benzyloxycarbonyl-prolyl-leucylglycyl-hydroxamate
-
1 mM, isoform LAST, 83% residual activity, isoform LAST_MAM, 15.5% resiudal activity
Carbobenzoxy-Phe-PSI(PO2H)-Ala-Pro-Phe-NH2
-
-
cystatin C
-
-
-
cysteine
-
weak
Dansyl-Gly-Lys-Arg
-
dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val as substrate
Dansyl-Leu-Lys-Arg
-
dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val as substrate
Diethyldicarbamate
-
-
dipicolinic acid
-
r
Ecballium elaterium Astacus protease inhibitor 1
-
i.e. EEAPI1
-
Ecballium elaterium Astacus protease inhibitor 2
-
i.e. EEAPI2
-
fetiun-like protein
-
the enzyme circulates in the blood stream in complex with a specific protein inhibitor, formerly termed nephrosin inhibitor, which is a homologue of fetuin, a large plasma protein with many functions. Fish fetuin, like its mammalian counterpart fetuin A, contains cystatin-like domains and is related to cystatin C-like inhibitors of cysteine cathepsins
-
fetuin
-
following proteolytic processing in hematopoietic tissues, fetuin serves as an endogenous inhibitor of enzyme
-
fetuin-A
-
-
-
fluorenylmethyloxycarbonyl-Pro-Lys-Phe-PSI[PO2CH2]Ala-Pro-Leu-Val
-
-
Hydroxamylsuccinyl-Pro-Phe-Arg
-
-
Potato inhibitor d
-
i.e. PoI-d, astacin inhibitor from potato
-
Pro-Leu-Gly-hydroxamate inhibitor
-
enzyme binding structure modeling
-
Tyrosin hydroxamate
-
most effective hydroxamate inhibitor
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.327
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.044
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.154
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.954
2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.867 - 0.87
2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
0.281
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.029
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.306
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
-
0.209
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
-
0.116
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
-
0.194
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
-
0.085
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
-
1.86
azocasein
pH 7.5, 37°C, Vmax: 0.281 microM/min
-
0.48
Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val
-
-
56
Dansyl-Arg-Ala-Pro-Leu
-
-
0.39
Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly
-
-
5
Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val
-
-
0.017
Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val
-
-
0.015
Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val
-
-
0.4 - 0.52
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val
0.27
Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val
-
-
0.53
Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val
-
-
0.23
Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val
-
dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly
0.58
Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly
-
-
0.67
Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly
-
-
0.33
Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val
-
-
1
Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val
-
-
0.29
Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val
-
-
0.28
Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val
-
-
2
Dansyl-Lys-Arg-Ala-Pro-Leu
-
-
0.37
Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val
-
-
0.25
Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val
-
dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.1
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
15.1
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
44.4
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
2.8
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-4-nitrophenylalanine-Arg
Astacus astacus
-
-
14.3
2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
3.6
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
78.4
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
Astacus astacus
-
-
834
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
Astacus astacus
-
-
0.002
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
Astacus astacus
-
-
0.001
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
Astacus astacus
-
-
0.004
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
Astacus astacus
-
-
78.7
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
Astacus astacus
-
-
27.9
azocasein
Steinernema carpocapsae
D2KBH9
pH 7.5, 37°C
-
200
Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val
Astacus astacus
-
-
0.035
Dansyl-Arg-Ala-Pro-Leu
Astacus astacus
-
-
34
Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly
Astacus astacus
-
-
0.7
Dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly
Astacus astacus
-
-
20
Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
2.3
Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val
Astacus astacus
-
-
2.2
Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
39 - 56
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val
40
Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val
Astacus astacus
-
-
20
Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val
Astacus astacus
-
-
310
Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
130
Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly
Astacus astacus
-
-
2.7
Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly
Astacus astacus
-
-
43
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu
Astacus astacus
-
-
120
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val
Astacus astacus
-
-
210
Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
180
Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val
Astacus astacus
-
-
210
Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val
Astacus astacus
-
-
67
Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val
Astacus astacus
-
-
40
Dansyl-Lys-Arg-Ala-Pro-Leu
Astacus astacus
-
-
380
Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
190
Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val
Astacus astacus
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0018
9-fluorenylmethyloxycarbonyl-KFPCAPLV-OH
-
pH 8.0, 25°C
0.000036
9-fluorenylmethyloxycarbonyl-PKFPCAPLV-OH
-
pH 8.0, 25°C
0.0036
9fluorenylmethyloxycarbonyl-FPCAPLVOH
-
pH 8.0, 25°C
0.01
benzyloxycarbonyl-PKF-PSI[PO2CH2]-APL-O-methyl
-
pH 8.0, 25°C
0.58
benzyloxycarbonyl-PKF-PSI[PO2CH2]-GPL-O-methyl
-
pH 8.0, 25°C
0.011
benzyloxycarbonyl-PKK-PSI[PO2CH2]-APLV-O-methyl
-
pH 8.0, 25°C
0.014
benzyloxycarbonyl-Pro-Lys-Phe-PSI[PO2CH2]Ala-Pro-O-methyl
-
pH 8.0, 25°C
0.000042
fluorenylmethyloxycarbonyl-Pro-Lys-Phe-PSI[PO2CH2]Ala-Pro-Leu-Val
-
pH 8.0, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
Ala-Ala-Ala 4-nitroanilide
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
assay at
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isoform LAST
Manually annotated by BRENDA team
-
isoform LAST
Manually annotated by BRENDA team
the enzyme Xhe2 is exclusively expressed in hatching gland cells as they first emerge at the lateral edge of the anterior neural plate, and persists in this tissue up to the tadpole stage
Manually annotated by BRENDA team
astacin is synthesized as a preproenzyme in the midgut gland
Manually annotated by BRENDA team
-
LAST_MAM signals are detected in all investigated tissues, most dominantly in the proventriculus and muscle
Manually annotated by BRENDA team
the proenzyme is only transiently found within the ducts between the hepatopancreas and the stomach
Manually annotated by BRENDA team
-
LAST_MAM signals are detected in all investigated tissues, most dominantly in the proventriculus and muscle
Manually annotated by BRENDA team
-
deposure of fed astacin
Manually annotated by BRENDA team
-
deposure of fed astacin
Manually annotated by BRENDA team
mature enzyme
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22180
-
calculation from sequence of cDNA
22610
-
Astacus astacus, deduced from amino acid sequence
35200
calculated from cDNA
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 26700, about, sequence calculation
monomer
-
1 * 23000, Astacus astacus, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
phosphoprotein
the protease has a putative signal peptide, 11 potential phosphorylation sites, and two disulfide bridges revealed by computational analysis
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
astacin structure compared to those of thermolysin and adamalysin II; Astacus astacus
-
Astacus astacus
-
Astacus astacus; X-ray crystallography
-
Astacus astacus; X-ray crystallography of Cu(II)-astacin, Co(II)-astacin and Ni(II)-astacin
-
crystal structure analysis, PDB ID 3LQ0
proastacin is crystallized by the sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 0.05 M MES, pH 5.6
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Remarkably stable against proteolytic digestion and autolysis
-
Soluble at concentrations of at least up to 20 mg/ml
-
Very resistant to self-degradation even upon prolonged incubation for several days
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, apoenzyme, at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
multiple immunologically identical forms
-
proastacin is purified by Ni-NTA column chromatography and HEMA-Bio gel filtration
recombinant astacin
-
two closely related forms of slightly different electrophoretic mobility
-
using Ni-NTA chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA reverse-transcribed from midgut-gland mRNA, cloned into pET3a vector and used to transform Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
expression in Hi5 cell
-
gene Ace-mtp-2, DNA and amino acid seuence determination and analysis, recombinant expression in Escherichia coli strains BL21 and Rosetta and Pichia pastoris strain X33
gene dpy-31, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis
gene xh2, DNA and amino acid sequence determination and analysis, sequence comparison, expression analysis
recombinantly expressed as a His-tagged fusion in Escherichia coli
recombinantly expressed in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information