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Information on EC 3.4.24.14 - procollagen N-endopeptidase and Organism(s) Homo sapiens

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.14 procollagen N-endopeptidase
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
Cleaves the N-propeptide of collagen chain alpha1(I) at Pro-/-Gln and of alpha1(II) and alpha2(I) at Ala-/-Gln
Synonyms
adamts2, adamts-2, adamts3, adamts14, procollagen n-proteinase, adamts 3, procollagen i n-proteinase, aminoprocollagen peptidase, adam-ts2, procollagen n-protease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADAM-TS2
ADAMTS proteinase
i.e. a disintegrin and metalloproteinase with thrombospondin type-1 motifs proteinase
ADAMTS-2
-
-
ADAMTS2
aminoprocollagen endopeptidase
-
aminoprocollagen peptidase
-
-
-
-
aminoterminal procollagen peptidase
-
-
-
-
PC I-NP
-
-
-
-
pNPI
-
-
-
-
procollagen ADAMTS
-
procollagen aminopeptidase
-
-
-
-
procollagen aminoterminal protease
-
-
-
-
Procollagen I N-proteinase
Procollagen I/II amino-propeptide processing enzyme
-
-
-
-
procollagen I/II N-endopeptidase
-
-
procollagen III N-endopeptidase
-
-
procollagen III N-proteinase
-
-
Procollagen N-endopeptidase
-
-
-
-
procollagen N-protease
procollagen N-proteinase
procollagen N-terminal peptidase
-
-
-
-
procollagen N-terminal proteinase
-
-
-
-
Procollagen peptidase
type I/II procollagen N-proteinase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
68651-94-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADAM10 + H2O
?
show the reaction diagram
-
-
-
?
ADAMTS1 + H2O
?
show the reaction diagram
-
-
-
?
C3a-R + H2O
?
show the reaction diagram
-
-
-
?
CTGF + H2O
?
show the reaction diagram
-
-
-
?
decorin + H2O
?
show the reaction diagram
-
-
-
?
DKK3 + H2O
?
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
release of the 33 kDa fibronectin fragment
-
-
?
fibulin-2 + H2O
?
show the reaction diagram
-
-
-
?
fibulin-3 + H2O
?
show the reaction diagram
-
-
-
?
HLA-A + H2O
?
show the reaction diagram
-
-
-
?
IGFBP5 + H2O
?
show the reaction diagram
-
-
-
?
LTBP1 + H2O
?
show the reaction diagram
LTBP2 + H2O
?
show the reaction diagram
-
-
-
?
lysyl oxidase + H2O
?
show the reaction diagram
-
-
-
?
lysyl oxidase homologue 1 + H2O
?
show the reaction diagram
-
-
-
?
perlecan + H2O
?
show the reaction diagram
-
-
-
?
POTEJ + H2O
?
show the reaction diagram
-
-
-
?
pro-collagen + H2O
collagen + collagen N-propeptide
show the reaction diagram
-
-
-
?
procollagen I + H2O
?
show the reaction diagram
-
-
-
?
procollagen I + H2O
pCCollagen I + oligopeptide
show the reaction diagram
-
-
-
-
?
procollagen II + H2O
?
show the reaction diagram
-
-
-
?
procollagen II + H2O
pCCollagen II + oligopeptide
show the reaction diagram
-
-
-
-
?
procollagen III + H2O
?
show the reaction diagram
-
-
-
?
procollagen V + H2O
?
show the reaction diagram
-
-
-
?
prosaposin + H2O
?
show the reaction diagram
-
-
-
?
TGF-beta RIII + H2O
?
show the reaction diagram
thrombospondin-1 + H2O
?
show the reaction diagram
-
-
-
?
type I aminoprocollagen + H2O
?
show the reaction diagram
type I procollagen + H2O
?
show the reaction diagram
type II procollagen + H2O
?
show the reaction diagram
type III procollagen + H2O
?
show the reaction diagram
type IV procollagen + H2O
?
show the reaction diagram
type V procollagen + H2O
?
show the reaction diagram
type XVIII procollagen + H2O
?
show the reaction diagram
cleavage of collagen alpha-1(XVIII) chain
-
-
?
versican core protein + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pro-collagen + H2O
collagen + collagen N-propeptide
show the reaction diagram
-
-
-
?
procollagen I + H2O
?
show the reaction diagram
-
-
-
?
procollagen II + H2O
?
show the reaction diagram
-
-
-
?
procollagen III + H2O
?
show the reaction diagram
-
-
-
?
procollagen V + H2O
?
show the reaction diagram
-
-
-
?
type I aminoprocollagen + H2O
?
show the reaction diagram
-
-
-
?
type I procollagen + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TIMP-3
-
inhibits ADAMTS-2 in vitro in the presence of procollagen I and II, addition of heparin enhances the ability of TIMP-3 to inhibit the enzyme
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 0.602
TIMP-3
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
assay at
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
upregulated expression of ADAMTS2 in failing human hearts, expression analysis of ADAMTS2 in human heart samples from patients with dilated cardiomyopathy and from healthy donors
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ATS2_HUMAN
1211
0
134755
Swiss-Prot
Secretory Pathway (Reliability: 1)
ATS3_HUMAN
1205
0
135603
Swiss-Prot
-
ATS14_HUMAN
1223
0
133888
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
118000
-
immunoprecipitation, truncated form
132000
-
immunoprecipitation, full-length mature form
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from cell-free extract by concanavalin A affinity chromatography, elution with alpha-methyl-D-mannoside, followed by heparin affinity chromatography and dialysis
partially purified
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ADAMTS14, alternative splicing is possible
gene ADAMTS14, recombinant expression in HEK-293 cells
gene ADAMTS2, recombinant expression in HEK-293 cells
gene ADAMTS2, sequence comparisons, alternative splicing is possible
gene ADAMTS2, two isozymes from alternative splicing mechanism. Beside the classical 1211 amino acid full length enzyme, a shorter form has been described. It is formed by the first 543 amino acids of the long form (corresponding to exons 1-10) followed by 23 amino acids encoded by an alternative exon present in intron 10 of the long form
gene ADAMTS3, recombinant expression in HEK-293 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme exxpression is upregulated by glucocorticoids
enzyme synthesis is increased by TGF beta in vitro and in fibrotic lesions in vivo. ADAMTS2 overexpression by macrophages and peripheral blood monocytes is stimulated by glucocorticoids
upregulated expression of ADAMTS2 in failing human hearts
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wang, W.M.; Ge, G.; Lim, N.H.; Nagase, H.; Greenspan, D.S.
TIMP-3 inhibits the procollagen N-proteinase ADAMTS-2
Biochem. J.
398
515-519
2006
Homo sapiens
Manually annotated by BRENDA team
Colige, A.
Procollagen III N-proteinase
Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
458-460
2004
Bos taurus, Homo sapiens
-
Manually annotated by BRENDA team
Colige, A.
Procollagen N-endopeptidase, ADAMTS2
Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
737-740
2004
Bos taurus, Gallus gallus, Homo sapiens
-
Manually annotated by BRENDA team
Bekhouche, M.; Colige, A.
The procollagen N-proteinases ADAMTS2, 3 and 14 in pathophysiology
Matrix Biol.
44-46C
46-53
2015
Bos taurus (E1BC50), Bos taurus (E1BFV4), Bos taurus (P79331), Canis lupus familiaris (E2R8G2), Gallus gallus, Homo sapiens (O15072), Homo sapiens (O95450), Homo sapiens (Q8WXS8), Mus musculus (Q8C9W3), Ovis aries (W5P0Z2), Rattus norvegicus (D3ZTE7), Sus scrofa (I3LAK9)
Manually annotated by BRENDA team
Bekhouche, M.; Leduc, C.; Dupont, L.; Janssen, L.; Delolme, F.; Vadon-Le Goff, S.; Smargiasso, N.; Baiwir, D.; Mazzucchelli, G.; Zanella-Cleon, I.; Dubail, J.; De Pauw, E.; Nusgens, B.; Hulmes, D.J.; Moali, C.; Colige, A.
Determination of the substrate repertoire of ADAMTS2, 3, and 14 significantly broadens their functions and identifies extracellular matrix organization and TGF-beta signaling as primary targets
FASEB J.
30
1741-1756
2016
Homo sapiens (O15072), Homo sapiens (O95450), Homo sapiens (Q8WXS8), Homo sapiens
Manually annotated by BRENDA team
Wang, X.; Chen, W.; Zhang, J.; Khan, A.; Li, L.; Huang, F.; Qiu, Z.; Wang, L.; Chen, X.
Critical role of ADAMTS2 (a disintegrin and metalloproteinase with thrombospondin motifs 2) in cardiac hypertrophy induced by pressure overload
Hypertension
69
1060-1069
2017
Homo sapiens (O95450), Homo sapiens, Mus musculus (Q8C9W3), Mus musculus, Rattus norvegicus (P16636)
Manually annotated by BRENDA team
Bekhouche, M.; Colige, A.
The procollagen N-proteinases ADAMTS2, 3 and 14 in pathophysiology
Matrix Biol.
44-46
46-53
2015
Homo sapiens (O15072), Homo sapiens (O95450), Homo sapiens (Q8WXS8), Mus musculus (Q8C9W3)
Manually annotated by BRENDA team
Colige, A.C.
Purification of native or recombinant ADAMTS2, and procollagen I cleavage assay
Methods Mol. Biol.
2043
55-62
2020
Homo sapiens (O95450), Homo sapiens, Bos taurus (P79331)
Manually annotated by BRENDA team
Sarioglu, N.; Erel, F.; Hismiogullari, A.A.; Cevik, C.
Association between the ADAMTS proteinases and obstructive sleep apnea
Sleep Breath.
24
835-840
2019
Homo sapiens (O15072), Homo sapiens
Manually annotated by BRENDA team