Information on EC 3.4.22.28 - picornain 3C

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The expected taxonomic range for this enzyme is: Viruses

EC NUMBER
COMMENTARY hide
3.4.22.28
-
RECOMMENDED NAME
GeneOntology No.
picornain 3C
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Selective cleavage of Gln-/-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
97162-88-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Schoenboeken
-
-
Manually annotated by BRENDA team
bovine herpesvirus 1 Schoenboeken
strain Schoenboeken
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
coxsackievirus CVB3
CVB3
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Enterovirus A71 E2004104-TW-CDC
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Enterovirus C Mahoney
type 1, i.e. Mahoney strain
-
-
Manually annotated by BRENDA team
Enterovirus C Sabin
type 2, i.e. Sabin strain
-
-
Manually annotated by BRENDA team
enterovirus CVB2
strain CVB2, CVB5, EV6, and EV9
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Enterovirus D68 3799
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Foot-and-mouth disease virus A Iran 97
strain A Iran 97
-
-
Manually annotated by BRENDA team
strain A1061
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-
Manually annotated by BRENDA team
strain A10_61
-
-
Manually annotated by BRENDA team
strain O/NY00
-
-
Manually annotated by BRENDA team
hepatitis A virus
-
-
-
Manually annotated by BRENDA team
human norovirus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2-(N-methylamino)benzoyl)-IEALFQGPPK-(2,4-dinitrophenyl)FR + H2O
(2-(N-methylamino)benzoyl)-IEALFQ + GPPK-(2,4-dinitrophenyl)FR
show the reaction diagram
most efficient substrate
-
-
?
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate + H2O
?
show the reaction diagram
hepatitis A virus
-
slowly turned over
-
-
?
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl + H2O
2-(N-methylamino)benzoyl-DEAMEQ + GVSDYIK-2,4-dinitrophenyl
show the reaction diagram
-
-
-
?
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IAALFQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
-
-
-
?
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IEAAFQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
2-(N-methylamino)benzoyl-IEALAQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IEALAQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
poor substrate
-
-
?
2-(N-methylamino)benzoyl-IEALFQSPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IEALFQ + SPPK(2,4-dinitrophenyl)FR
show the reaction diagram
poor substrate
-
-
?
2-(N-methylamino)benzoyl-IEALKQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IEALKQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
poor substrate
-
-
?
2-(N-methylamino)benzoyl-IEFLFQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IEFLFQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
poor substrate
-
-
?
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR + H2O
2-(N-methylamino)benzoyl-IELLFQ + GPPK(2,4-dinitrophenyl)FR
show the reaction diagram
2-(N-methylamino)benzoyl-LFAGFQGAYSGAK-2,4-dinitrophenyl + H2O
2-(N-methylamino)benzoyl-LFAGFQ + GAYSGAK-2,4-dinitrophenyl
show the reaction diagram
-
-
-
?
2-(N-methylamino)benzoyl-QTGTIQGDRVADK-2,4-dinitrophenyl + H2O
2-(N-methylamino)benzoyl-QTGTIQ + GDRVADK-2,4-dinitrophenyl
show the reaction diagram
poor substrate
-
-
?
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl + H2O
2-(N-methylamino)benzoyl-RQAVTQ + GFPTELK-2,4-dinitrophenyl
show the reaction diagram
-
-
-
?
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl + H2O
2-(N-methylamino)benzoyl-RTATVQ + GPSLDFK-2,4-dinitrophenyl
show the reaction diagram
-
-
-
?
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met + H2O
2-(N-methylamino)benzoyl-TSAVLQ + SGFRK-(2,4-dinitrophenyl)-Met
show the reaction diagram
-
-
-
?
2-aminobenzoic acid-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Phe(NO2)-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoic acid-Phe-Thr-Gln-Ser-Glu-Gly-Glu-Phe(NO2)-Ala + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
2-aminobenzoyl-AALFQGPLQ-EDDnp + H2O
2-aminobenzoyl-AALFQ + GPLQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-EAAFQGPLQ-EDDnp + H2O
2-aminobenzoyl-EAAFQ + GPLQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-EALFQAPLQ-EDDnp + H2O
2-aminobenzoyl-EALFQ + APLQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-EALFQGALQ-EDDnp + H2O
2-aminobenzoyl-EALFQ + GALQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-EALFQGPAQ-EDDnp + H2O
2-aminobenzoyl-EALFQ + GPAQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-EALFQGPLQ-EDDnp + H2O
2-aminobenzoyl-EALFQ + GPLQ-EDDnp
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln-Gly-Pro-(4-nitro)Phe + H2O
2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln + Gly-Pro-(4-nitro)Phe
show the reaction diagram
-
synthetic peptide spanning the 2C/3A cleavage region of poliovirus polyprotein
-
?
4-(4-dimethylaminophenylazo)benzoic acid-KTSAVLQSGFRKME-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoic acid-KTSAVLQ + SGFRKME-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
fluorogenic substrate
-
-
?
5-bromopyridin-3-yl furan-3-carboxylate + H2O
?
show the reaction diagram
hepatitis A virus
-
slowly turned over
-
-
?
5-chloropyridin-3-yl furan-2-carboxylate + H2O
?
show the reaction diagram
hepatitis A virus
-
slowly turned over
-
-
?
7-methoxycoumarin-4-yl-MEALFQGPLQY-2,4-dinitrophenyl amide + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-EALFQGPLQ-EDDnp + H2O
Abz-EALFQ + GPLQ-EDDnp
show the reaction diagram
-
-
-
-
?
abz-SVTLQSGY(NO2)R + H2O
?
show the reaction diagram
hepatitis A virus
-
fluorogenic substrate
-
-
?
Ac-DEFQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Ac-EFQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Ac-FQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Ac-RHSVGATLEALFQGPPVYREIKIS-NH2 + H2O
Ac-RHSVGATLEALFQ + GPPVYREIKIS-NH2
show the reaction diagram
-
-
-
-
?
acetyl-Arg-Pro-Val-Val-Val-Gln-Gly-Pro-Asn-NH2 + H2O
?
show the reaction diagram
-
human rhinovirus
-
-
?
acetyl-Arg-Ser-Tyr-Phe-Ala-Gln-Ile-Gln-Gly-Glu-Ile-Gln-Trp-Met-Arg-Pro-NH2 + H2O
acetyl-Arg-Ser-Tyr-Phe-Ala-Gln-Ile-Gln + Gly-Glu-Ile-Gln-Trp-Met-Arg-Pro-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 3C/3D
-
?
acetyl-Asn-Cys-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O
acetyl-Asn-Cys-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 2C/3A
-
?
acetyl-ELRTQSFS-NH2 + H2O
?
show the reaction diagram
hepatitis A virus
-
-
-
-
?
acetyl-Glu-Ala-Leu-Phe-Gln-Gly-Gly-NH2 + H2O
acetyl-Glu-Ala-Leu-Phe-Gln + Gly-Gly
show the reaction diagram
-
as good as acetyl-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Pro-Val
-
?
acetyl-Glu-Ala-Leu-Phe-Gln-Gly-NH2 + H2O
acetyl-Glu-Ala-Leu-Phe-Gln + Gly-NH2
show the reaction diagram
-
-
-
?
acetyl-Glu-Ala-Leu-Phe-Gln-Gly-Pro-NH2 + H2O
acetyl-Glu-Ala-Leu-Phe-Gln + Gly-Pro
show the reaction diagram
-
-
-
?
acetyl-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Pro-Val + H2O
acetyl-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Pro-Val
show the reaction diagram
-
sequence corresponding to 2C/3A cleavage site of polyprotein
-
?
acetyl-Glu-Ala-Leu-Phe-Gln-NH2 + H2O
acetyl-Glu-Ala-Leu-Phe-Gln + NH3
show the reaction diagram
-
cleavage with reduced but significant efficiency, better substrate than acetyl-Glu-Ala-Leu-Phe-Gln-Gly-NH2
-
?
acetyl-Glu-Ile-Pro-Tyr-Ala-Ile-Glu-Gln-Gly-Asp-Ser-Trp-Leu-Lys-Lys-Phe-NH2 + H2O
acetyl-Glu-Ile-Pro-Tyr-Ala-Ile-Glu-Gln + Gly-Asp-Ser-Trp-Leu-Lys-Lys-Phe-NH2
show the reaction diagram
-
best substrate, corresponding to poliovirus type 2 polyprotein cleavage site 2B/2C
-
?
acetyl-Glu-Leu-Arg-Thr-Gln-Ser-Phe-Ser-NH2 + H2O
?
show the reaction diagram
acetyl-Ile-Arg-Thr-Ala-Lys-Val-Gln-Gly-Pro-Gly-Phe-Asp-Tyr-Ala-Val-NH2 + H2O
acetyl-Ile-Arg-Thr-Ala-Lys-Val-Gln + Gly-Pro-Gly-Phe-Asp-Tyr-Ala-Val-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 3B/3C
-
?
acetyl-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O
acetyl-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2
show the reaction diagram
acetyl-Thr-Ile-Arg-Thr-Ala-Lys-Val-Gln-Gly-Pro-Gly-Phe-Asp-Tyr-Ala-Val-NH2 + H2O
acetyl-Thr-Ile-Arg-Thr-Ala-Lys-Val-Gln + Gly-Pro-Gly-Phe-Asp-Tyr-Ala-Val-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 3B/2C
-
?
acetyl-Tyr-Glu-Glu-Glu-Ala-Met-Glu-Gln-Gly-Ile-Ser-Asn-Tyr-Ile-Glu-Ser-NH2 + H2O
acetyl-Tyr-Glu-Glu-Glu-Ala-Met-Glu-Gln + Gly-Ile-Ser-Asn-Tyr-Ile-Glu-Ser-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 2A/2B
-
?
acetyl-Tyr-Lys-Leu-Phe-Ala-Gly-His-Gln-Gly-Ala-Tyr-Thr-Gly-Leu-Phe-Asn-NH2 + H2O
acetyl-Tyr-Lys-Leu-Phe-Ala-Gly-His-Gln + Gly-Ala-Tyr-Thr-Gly-Leu-Phe-Asn-NH2
show the reaction diagram
-
corresponding to poliovirus type 2 polyprotein cleavage site 3A/3B
-
?
activated transcription factor TFIIIC + H2O
?
show the reaction diagram
APAKELLNFD + H2O
APAKE + LLNFD
show the reaction diagram
-
-
-
-
?
APAKQLLNFD + H2O
APAKQ + LLNFD
show the reaction diagram
-
-
-
-
?
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu + H2O
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln + Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu
show the reaction diagram
-
represents 2C/3A cleavage site, wild-type and E71D, D85N, K155E mutants, not other mutants
-
?
Bid protein + H2O
?
show the reaction diagram
-
-
-
-
?
cAMP-regulated response element binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
cAMP-responsible element binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
cardiovirus polyprotein + H2O
?
show the reaction diagram
CstF-64 protein + H2O
?
show the reaction diagram
-
CstF-64 protein is short for cleavage stimulation factor, 39 pre-RNA, subunit 2, 64 kD. The 3Cpro cleavage sites are located at position 251 in the N-terminal P/G-rich domain and at multiple positions close to the C-terminus of CstF-64 (around position 500)
-
-
?
Dabcyl-APAKQLLD(Edans)FDLLK + H2O
Dabcyl-APAKQ + LLD(Edans)FDLLK
show the reaction diagram
-
-
-
-
-
Dabcyl-GLRTQSFS-EDANS + H2O
?
show the reaction diagram
hepatitis A virus
-
fluorogenic substrate, hydrolysis at the glutamine residue
-
-
?
dabcyl-GLRTQSND(EDANS)G + H2O
?
show the reaction diagram
hepatitis A virus
-
fluorogenic substrate
-
-
?
Dabcyl-IEALFQGPPKFRE-Edans + H2O
Dabcyl-IEALFQ + GPPKFRE-Edans
show the reaction diagram
-
-
-
?
Dabcyl-KEALFQGPPQFE-Edans + H2O
Dabcyl-KEALFQ + GPPQFE-Edans
show the reaction diagram
Dabcyl-KIGNTIEALFQGPPKFRE-EDANS + H2O
Dabcyl-KIGNTIEALFQ + GPPKFRE-EDANS
show the reaction diagram
Dabcyl-KRTATVQGPSLDFE-Edans + H2O
Dabcyl-KRTATVQ + GPSLDFE-Edans
show the reaction diagram
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O
Dabcyl-KTSAVL + QSGFRKME-EDANS
show the reaction diagram
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O
Dabcyl-KTSAVLQ + SGFRKME-EDANS
show the reaction diagram
Dabcyl-RTATVQGPSLDFE-EDANS + H2O
Dabcyl-RTATVQ + GPSLDFE-EDANS
show the reaction diagram
-
-
-
?
Dabcyl-RTATVQGPSLDFKE-Edans + H2O
Dabcyl-RTATVQ + GPSLDFKE-Edans
show the reaction diagram
-
-
-
?
Dabcyl-TAKVQGPGFD-Edans + H2O
Dabcyl-TAKVQ + GPGFD-Edans
show the reaction diagram
Dabcyl-TSAVLQSGFRKM-Edans + H2O
Dabcyl-TSAVLQ + SGFRKM-Edans
show the reaction diagram
poor substrate
-
-
?
dansyl-Glu-Glu-Glu-Ala-Met-Glu-Gly-Ile-Thr-Asn-Lys-NH2 + H2O
dansyl-Glu-Glu-Glu-Ala-Met-Glu + Gly-Ile-Thr-Asn-Lys-NH2
show the reaction diagram
DEAMEQGVSDYI + H2O
DEAMEQ + GVSDYI
show the reaction diagram
-
-
-
-
?
DRRETLFQ-4-nitroanilide + H2O
DRRETLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
DSLETLFQ-4-nitroanilide + H2O
DSLETLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
DSLEVLFQ-4-nitroanilide + H2O
DSLEVLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
DSRETLFQ-4-nitroanilide + H2O
DSRETLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
EALFQ-4-nitroanilide + H2O
EALFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Edans-DFHLQGP-Dabcyl + H2O
Edans-DFHLQ + GP-Dabcyl
show the reaction diagram
-
-
-
-
?
EDANS-EDFHLQGPEDLAK-Dabcyl + H2O
EDANS-EDFHLQ + GPEDLAK-Dabcyl
show the reaction diagram
-
-
-
-
?
eIF4GI + H2O
?
show the reaction diagram
-
cleavage in baby hamster kidney cells and 3T3 cells, cleavage can occur either on the full-length protein or on the primary cleavage product that is generated by Lpro, human and green monkey eIF4GI is resistant to the action of 3Cpro, a single amino acid substitution renders human eIF4GI sensitive to 3Cpro
-
-
?
encephalomyocarditis virus polyprotein + H2O
?
show the reaction diagram
-
cleavage sites: Gln-Gly, Glu-Ser and Glu-Ala
-
-
?
enterovirus polyprotein + H2O
?
show the reaction diagram
ERGIC53 protein + H2O
?
show the reaction diagram
-
-
-
-
?
ETLFQ-4-nitroanilide + H2O
ETLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
eukaryotic initiation factor + H2O
eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-?
show the reaction diagram
eukaryotic translation initiation factor 4Gl + H2O
?
show the reaction diagram
-
-
-
-
?
EVLFQ-4-nitroanilide + H2O
EVLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
FAGLAQAVTQGFPTEL + H2O
FAGLAQAVTQ + GFPTEL
show the reaction diagram
FAGLRAAVTQGFPTEL + H2O
FAGLRAAVTQ + GFPTEL
show the reaction diagram
FAGLRQAVEQGFPTEL + H2O
FAGLRQAVEQ + GFPTEL
show the reaction diagram
FAGLRQAVFQGFPTEL + H2O
FAGLRQAVFQ + GFPTEL
show the reaction diagram
FAGLRQAVKQGFPTEL + H2O
FAGLRQAVKQ + GFPTEL
show the reaction diagram
FAGLRQAVKQGIPTEL + H2O
FAGLRQAVKQ + GIPTEL
show the reaction diagram
FAGLRQAVTQGFPTEL + H2O
FAGLRQAVTQ + GFPTEL
show the reaction diagram
FAGLRQAVTQSFPTEL + H2O
FAGLRQAVTQ + SFPTEL
show the reaction diagram
Foot-and-mouth disease virus polyprotein + H2O
?
show the reaction diagram
foot-and-mouth-disease virus P1 precursor polyprotein + H2O
mature structural foot-and-mouth-disease virus proteins
show the reaction diagram
-
recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1 processing
VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme)
?
foot-and-mouth-disease virus P1-2A polyprotein + H2O
mature structural foot-and-mouth-disease virus proteins
show the reaction diagram
-
recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site initially, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1-processing
VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme)
?
foot-and-mouth-disease virus P2 precursor protein + H2O
foot-and-mouth-disease virus protein 2B + foot-and-mouth-disease virus protein 2C + foot-and-mouth-disease virus protein 2A-2B
show the reaction diagram
-
-
-
?
Glu-Ala-Ile-Ala-Glu-Glu-Gln-Gly-Leu-Ser-Asp-Tyr-Ile-Thr + H2O
?
show the reaction diagram
-
synthetic peptide spanning the 2A/2B cleavage region of human rhinovirus polyprotein
-
-
?
Glu-Ala-Leu-Phe-Gln-4-nitroanilide + H2O
Glu-Ala-Leu-Phe-Gln + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Glu-Ala-Leu-Phe-Gln-Gly-Pro-Pro-Val + H2O
Glu-Ala-Leu-Phe-Gln + Gly-Pro-Pro-Val
show the reaction diagram
-
represents 2C/3A cleavage site, wild-type and C147S mutant, the latter with very reduced activity
-
?
hepatitis A virus deltaP1-2AB polyprotein fragment + H2O
hepatitis A virus protein 2A + hepatitis A virus proteins deltaVP1-2A + hepatitis A virus proteins 2BC + hepatitis A virus protein 2 + hepatitis A virus protein 2B
show the reaction diagram
hepatitis A virus
-
C-terminally truncated protein
-
?
hepatitis A virus deltaVP1-P2-P3 polyprotein fragment + H2O
hepatitis A virus proteins2BC + hepatitis A virus proteins P3* + hepatitis A virus proteins 2B + hepatitis A virus proteins deltaVP1-2A + hepatitis A virus proteins 2A
show the reaction diagram
hepatitis A virus deltaVP1-P2-P3*my polyprotein fragment + H2O
hepatitis A virus protein 2BC + hepatitis A virus proteins P3*my + hepatitis A virus proteins 2B + hepatitis A virus proteins deltaVP1-2A + hepatitis A virus proteins 2A
show the reaction diagram
hepatitis A virus
-
C-terminally truncated protein, proteolytically inactive substrate, kinetics, yields the same products as active polyprotein precursor deltaVP1-P2-P3*
P3my and deltaVP1-2A are intermediates
?
hepatitis A virus P1-2AB* polyprotein fragment + H2O
hepatitis A virus protein VP3 + hepatitis A virus proteins VP0 + hepatitis A virus proteins VP1-2A + hepatitis A virus proteins VP0-VP3 + hepatitis A virus proteins P1-2A + hepatitis A virus proteins 2A
show the reaction diagram
hepatitis A virus
-
C-terminally truncated protein, cleavage sites: VPO/VP3, VP3/VP1, 2A/2B, less efficient are VP1/2A and 2B/2C, via 2BC
-
?
hepatitis A virus P1-2ABC* polyprotein fragment + H2O
hepatitis A virus protein VP3 + hepatitis A virus proteins VP0 + hepatitis A virus proteins VP1-2A + hepatitis A virus proteins VP0-VP3 + hepatitis A virus proteins P1-2A + hepatitis A virus proteins 2A + hepatitis A virus proteins 2BC
show the reaction diagram
hepatitis A virus
-
C-terminally truncated protein, cleavage sites: VPO/VP3, VP3/VP1, 2A/2B, less efficient are VP1/2A and 2B/2C, via 2BC
-
?
hepatitis A virus P1-P2 precursor polyprotein + H2O
hepatitis A virus protein 2A + hepatitis A virus protein 2B + hepatitis A virus protein 2C + hepatitis A virus protein 3ABC + hepatitis A virus protein 3D
show the reaction diagram
hepatitis A virus
-
most effective cleavage at 2A/2B and 2C/3A sites
via respective intermediate products
?
hepatitis A virus P2-P3 precursor polyprotein + H2O
?
show the reaction diagram
hepatitis A virus
-
trans-cleavage, rapid cleavage at P2/P3 junction, the 3A/3B, 3B/3C and 3C/3D sites are less efficiently cleaved, the hepatitis A virus enzyme has a broader activity profile than enzymes of other picornaviruses, recombinant enzyme cleaves all putative cleavage sites of the polyprotein
-
-
?
hepatitis A virus polyprotein + H2O
?
show the reaction diagram
hepatitis A virus polyprotein + H2O
hepatitis A virus protein P1-2A + hepatitis A virus protein P3 + hepatitis A virus protein 2BC + hepatitis A virus protein 3D + hepatitis A virus protein 3ABC
show the reaction diagram
hepatitis A virus
-
cleavage of P2-P3 domain, cleaves primary cleavage site and all secondary sites within P2 as well as the 3C/3D junction
followed by hepatitis A virus protein 2B and hepatitis A virus protein 2C and later by hepatitis A virus protein VP0 and hepatitis A virus protein VP3, enzyme-concentration dependent product formation
?
histone H3 protein + H2O
?
show the reaction diagram
-
-
-
-
?
histone protein H3 + H2O
?
show the reaction diagram
-
in BHK-cells, foot-and-mouth-disease virus enzyme
-
-
?
human rhinovirus 3CD polyprotein fragment + H2O
?
show the reaction diagram
-
recombinant enzyme HRV-14, structure/mechanism study
-
-
?
human rhinovirus polyprotein + H2O
1AB/1C/1D/2C/delta3CD
show the reaction diagram
-
-
-
-
?
IEALFQGPPKFR + H2O
IEALFQ + GPPKFR
show the reaction diagram
-
-
-
-
?
IGNTIEALFQGPPKFR + H2O
IGNTIEALFQ + GPPKFR
show the reaction diagram
ILGIPIVQKQSASWLK + H2O
ILGIPIVQKQ + SASWLK
show the reaction diagram
in vitro-translated (35S)methionine-labeled transcription factor cyclic AMP-responsive element binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
interferon regulatory factor 7 + H2O
?
show the reaction diagram
-
cleavage occurs at the Q189-S190 junction within the constitutive activation domain
-
-
?
KPVLRTATVQGPSLDF + H2O
KPVLRTATVQ + GPSLDF
show the reaction diagram
L-P1-2A precursor polypeptide of encephalomyocarditis virus + H2O
peptide P1-2A
show the reaction diagram
-
cardioviruses
-
?
LKRGYFASEQGEIQWM + H2O
LKRGYFASEQ + GEIQWM
show the reaction diagram
LKRSYFASEQGEIQWV + H2O
LKRSYFASEQ + GEIQWV
show the reaction diagram
LWLDEEAMEQGVSDYI + H2O
LWLDEEAMEQ + GVSDYI
show the reaction diagram
Lys-Gly-Leu-Phe-Ser-Gln-Ala-Lys-Ile-Ser-Leu-Phe-Tyr-Thr + H2O
Lys-Gly-Leu-Phe-Ser-Gln + Ala-Lys-Ile-Ser-Leu-Phe-Tyr-Thr
show the reaction diagram
hepatitis A virus
-
-
-
?
membrin + H2O
?
show the reaction diagram
-
-
-
-
?
mitochondrial antiviral signaling protein + H2O
?
show the reaction diagram
-
3Cpro-mediated MAVS cleavage occurs within its proline-rich region, leads to its relocalization from the mitochondrial membrane, and ablates its downstream signaling
-
-
?
N-acetyl-DEFQLQ-4-nitroanilide + H2O
N-acetyl-DEFQLQ + 4-nitroaniline
show the reaction diagram
human norovirus
-
-
-
-
-
nuclear factor-kappaB essential modulator + H2O
?
show the reaction diagram
hepatitis A virus
-
the enzyme cleaves the substrate at the Q304 residue, negating its signaling adaptor function
-
-
?
nucleoporin + H2O
?
show the reaction diagram
-
-
-
-
?
P1-2A polyprotein + H2O
hydrolyzed P1-2A polyprotein
show the reaction diagram
P1-2A precursor polypeptide of encephalomyocarditis virus + H2O
peptide P1 + peptide 2A
show the reaction diagram
-
cardio- and aphthoviruses
-
?
picornavirus polyprotein + H2O
hydrolyzed picornavirus polyprotein
show the reaction diagram
poliovirus 3ABC precursor polyprotein + H2O
poliovirus protein 3A + poliovirus protein 3B + poliovirus protein 3C
show the reaction diagram
poliovirus P1 precursor polyprotein + H2O
capsid proteins
show the reaction diagram
poliovirus polyprotein + H2O
hydrolyzed poliovirus polyprotein
show the reaction diagram
poliovirus polyprotein fragment corresponding to 2BC coding region + H2O
?
show the reaction diagram
poly(A) binding protein + H2O
?
show the reaction diagram
-
the enzyme cleaves poly(A) binding protein within the C-terminal domain at Q437/G438
-
-
?
poly(A) binding protein + H2O
fragments of poly(A) binding protein
show the reaction diagram
-
contains 2 main cleavage sites for 3C proteinase within the proline-rich linker domain, cleavage of recombinant protein with 3C proteinase heavily favors the 3Cpro primary cleavage site Q537/G538 over the 3CAlt cleavage site Q413/T414
-
-
?
Poly(A)-binding protein + H2O
?
show the reaction diagram
-
3Cpro removes the C-terminal domain that interacts with several translation factors, in HeLa cells 3Cpro inhibits translation of endogenous mRNAs and reporter RNA, 3Cpro-mediated translation inhibition can be restored by the addition of exogenous poly(A)-binding protein
-
-
r
poly(A)-binding protein + H2O
fragments of poly(A)-binding protein
show the reaction diagram
-
inhibition of viral internal ribosome entry site-mediated translation, cleavage of poly(A)-binding protein by 3Cpro is a necessary component for host translation shutoff
-
-
?
poly-(ADP-ribose) polymerase + H2O
?
show the reaction diagram
-
-
-
-
?
polypeptide translated from plasmid DNA T7-delta3A-3B-3Cmut-delta3D
delta3A-3B-3Cmut + 3Cmut-delta3D + 3B-3Cmut-delta3D
show the reaction diagram
-
-
-
-
?
pro-caspase 3 + H2O
caspase 3 + ?
show the reaction diagram
-
-
-
-
?
QTGTIQGDRVAD + H2O
QTGTIQ + GDRVAD
show the reaction diagram
-
-
-
-
?
Ras-GTPase activating protein SH3 domain-binding protein 1 + H2O
fragments of Ras-GTPase activating protein SH3 domain-binding protein 1
show the reaction diagram
-
-
-
-
?
rhinovirus polyprotein + H2O
?
show the reaction diagram
-
cleavage specificity
-
-
?
RNA polymerase I factor SL-1 + H2O
?
show the reaction diagram
-
-
-
-
?
RQAVTQGFPTEL + H2O
RQAVTQ + GFPTEL
show the reaction diagram
-
-
-
-
?
SLEVLFQGPVRR + H2O
SLEVLFQ + GPVRR
show the reaction diagram
-
-
-
-
?
TATA binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
TATA-binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
TENLYFQSGTRR + H2O
TENLYFQ + SGTRR
show the reaction diagram
-
-
-
-
?
Thr-Gly-Leu-Phe-Gln-Gly-Pro + H2O
Thr-Gly-Leu-Phe-Gln + Gly-Pro
show the reaction diagram
-
-
-
-
?
TLFQ-4-nitroanilide + H2O
TLFG + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Toll/IL-1 receptor domain-containing adaptor inducing interferon-beta + H2O
?
show the reaction diagram
-
3Cpro cleaves both the N- and C-terminal domains of TRIF and localizes with TRIF to signalosome complexes within the cytoplasm
-
-
?
transcription factor OCT-1 + H2O
?
show the reaction diagram
TRIF protein + H2O
?
show the reaction diagram
-
-
-
-
?
TSAVLQSGFRKM + H2O
TSAVLQ + SGFRKM
show the reaction diagram
-
-
-
-
?
TTLEALFQGPPVY + H2O
?
show the reaction diagram
-
-
-
-
?
viral 3CD protein + H2O
?
show the reaction diagram
-
-
-
-
?
VIYKLFAGFQGAYSGA + H2O
VIYKLFAGFQ + GAYSGA
show the reaction diagram
YFCSEQGEIQWN + H2O
YFCSEQ + GEIQWN
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
activated transcription factor TFIIIC + H2O
?
show the reaction diagram
-
in HeLa-cells, poliovirus enzyme, involved in shut-off of host-cell transcription
-
-
?
ERGIC53 protein + H2O
?
show the reaction diagram
-
-
-
-
?
eukaryotic initiation factor + H2O
eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-?
show the reaction diagram
hepatitis A virus polyprotein + H2O
?
show the reaction diagram
interferon regulatory factor 7 + H2O
?
show the reaction diagram
-
cleavage occurs at the Q189-S190 junction within the constitutive activation domain
-
-
?
membrin + H2O
?
show the reaction diagram
-
-
-
-
?
mitochondrial antiviral signaling protein + H2O
?
show the reaction diagram
-
3Cpro-mediated MAVS cleavage occurs within its proline-rich region, leads to its relocalization from the mitochondrial membrane, and ablates its downstream signaling
-
-
?
nuclear factor-kappaB essential modulator + H2O
?
show the reaction diagram
hepatitis A virus
-
the enzyme cleaves the substrate at the Q304 residue, negating its signaling adaptor function
-
-
?
picornavirus polyprotein + H2O
hydrolyzed picornavirus polyprotein
show the reaction diagram
poliovirus polyprotein + H2O
hydrolyzed poliovirus polyprotein
show the reaction diagram
poly(A) binding protein + H2O
?
show the reaction diagram
-
the enzyme cleaves poly(A) binding protein within the C-terminal domain at Q437/G438
-
-
?
poly(A) binding protein + H2O
fragments of poly(A) binding protein
show the reaction diagram
-
contains 2 main cleavage sites for 3C proteinase within the proline-rich linker domain, cleavage of recombinant protein with 3C proteinase heavily favors the 3Cpro primary cleavage site Q537/G538 over the 3CAlt cleavage site Q413/T414
-
-
?
poly(A)-binding protein + H2O
fragments of poly(A)-binding protein
show the reaction diagram
-
inhibition of viral internal ribosome entry site-mediated translation, cleavage of poly(A)-binding protein by 3Cpro is a necessary component for host translation shutoff
-
-
?
Ras-GTPase activating protein SH3 domain-binding protein 1 + H2O
fragments of Ras-GTPase activating protein SH3 domain-binding protein 1
show the reaction diagram
-
-
-
-
?
Thr-Gly-Leu-Phe-Gln-Gly-Pro + H2O
Thr-Gly-Leu-Phe-Gln + Gly-Pro
show the reaction diagram
-
-
-
-
?
Toll/IL-1 receptor domain-containing adaptor inducing interferon-beta + H2O
?
show the reaction diagram
-
3Cpro cleaves both the N- and C-terminal domains of TRIF and localizes with TRIF to signalosome complexes within the cytoplasm
-
-
?
TRIF protein + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
3C protease requires neither cofactors nor metals for activity
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cl-
-
activates
HPO42-
-
activates
NaCl
highest proteolytic activity is at 150 mM NaCl
additional information
-
Mn2+ or Ca2+ do not affect protease activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid
(2Z)-3-[4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinolin-6-yl]prop-2-enoic acid
-
70% effect at 0.1 mM
(3S)-[N3-(acetyl-L-leucyl-L-alanyl)-N1-[3'-(N,N-dimethylamino)-3'-oxopropyl]-N1-(methylsulfonyl)]-1,3-diaminobutan-2-one
hepatitis A virus
-
weak competitive
(3S)-[N3-(acetyl-L-leucyl-L-alanyl)-N1-[3'-(N,N-dimethylamino)-3'-oxopropyl]-N1-(p-methylphenylsulfonyl)]-1,3-diaminobutan-2-one
hepatitis A virus
-
weak competitive
(3S)-[N3-(benzyloxycarbonyl)-N1-[3'-(N,N-dimethylamino)-3'-oxoprolyl]-N1-(methylsulfonyl)]-1,3-diaminobutan-2-one
hepatitis A virus
-
weak competitive
(3S)-[N3-(benzyloxycarbonyl)-N1-[3'-(N,N-dimethylamino)-3'-oxopropyl]-N1-(p-methylphenylsulfonyl)]-1,3-diaminobutan-2-one
hepatitis A virus
-
weak competitive, IC50: 0.075 mM
(4S)-4-(acetylamino)-N,N-dimethyl-5-oxopentanamide
hepatitis A virus
-
hepatitis A virus
(5-bromopyridin-3-yl)methyl furan-2-carboxylate
hepatitis A virus
-
67% inhibition at 10 microM inhibitor concentration
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate
hepatitis A virus
-
83% inhibition at 10 microM inhibitor concentration, above 90% inhibition at 1 microM inhibitor concentration, 43% inhibition at 0.25 microM inhibitor concentration
1,3-Dibromoacetone
-
-
1,3-diphenyl-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-(benzyloxy)-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-3-(1H-imidazol-3-ium-2-yl)-L-alaninamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-5-ammonio-L-norvalinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-N5-[amino(iminio)methyl]-L-ornithinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-N6-[amino(iminio)methyl]-L-lysinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]glycinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-[methoxy(methyl)amino]-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
1-acetyl-L-prolyl-L-alanyl-N1-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-glutamamide
-
-
1-acetyl-L-prolyl-L-alanyl-N6-acetyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-lysinamide
-
-
1-acetyl-L-prolyl-N-[(2S)-1-[[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]amino]-4-ammonio-1-oxobutan-2-yl]-L-alaninamide
-
-
1-acetyl-L-prolyl-N-[(2S)-1-[[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]amino]-4-[[amino(iminio)methyl]amino]-1-oxobutan-2-yl]-L-alaninamide
-
-
1-benzoylprolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
1-benzylprolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
1-[(4-methylphenyl)sulfonyl]prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
13C-labeled beta-lactone
hepatitis A virus
-
-
-
2,2'-dipyridyl disulfide
-
0.4 mM, complete inhibition
2-(pyridin-3-yl)quinolin-4(1H)-one
-
35% effect at 0.1 mM
2-aminopyridin-3-yl furan-2-carboxylate
-
24% inhibition at 0.001 mM
2-carbamoylpyridin-3-yl furan-2-carboxylate
-
14% inhibition at 0.001 mM
2-chloropyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
11% inhibition at 10 microM inhibitor concentration
2-methylpyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 1 microM inhibitor concentration
2-oxo-1,2-dihydroquinolin-4-yl furan-2-carboxylate
-
98% inhibition at 0.001 mM
2-pyridin-3-yl-1-thiophen-2-ylethanone
hepatitis A virus
-
10% inhibition at 10 microM inhibitor concentration
3-(benzylamino)-5-[1-N-hydroxyethanimidoyl]benzamide
-
-
3-(bromoacetyl)-5-(naphth-2-ylmethyl)benzamide
-
12% inhibition at inhibitor concentration of 100 nM, 39% inhibition at inhibitor concentration of 1 microM, 83% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-(bromoacetyl)benzamide
-
4% inhibition at inhibitor concentration of 100 nM, 23% inhibition at inhibitor concentration of 1 microM, 85% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-(chloroacetyl)-5-(naphth-2-ylmethyl)benzamide
-
25% inhibition at inhibitor concentration of 100 nM, 61% inhibition at inhibitor concentration of 1 microM, 81% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-(chloroacetyl)benzamide
-
-
3-(dibromoacetyl)-5-(naphth-2-ylmethyl)benzamide
-
-
3-(dibromoacetyl)benzamide
-
-
3-(dichloroacetyl)benzamide
-
-
3-(difluoroacetyl)-5-[(phenylcarbonyl)amino]benzamide
-
-
3-(difluoroacetyl)benzamide
-
-
3-(trifluoroacetyl)benzamide
-
no inhibition of 3CP
3-(trifluoroacetyl)benzonitrile
-
-
3-acetyl-5-(5,8-dihydronaphthalen-2-ylmethyl)benzamide
-
-
3-acetyl-5-(naphth-1-ylcarbamoyl)benzoic acid
-
-
3-acetyl-5-(phenylcarbamoyl)benzoic acid
-
-
3-acetyl-5-benzylbenzamide
-
-
3-acetylbenzamide
-
-
3-amino-6-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-6-methyl-4-oxoheptanoic acid
-
-
3-benzyl-5-(bromoacetyl)benzamide
-
0% inhibition at inhibitor concentration of 100 nM, 30% inhibition at inhibitor concentration of 1 microM, 68% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-benzyl-5-(chloroacetyl)benzamide
-
14% inhibition at inhibitor concentration of 100 nM, 79% inhibition at inhibitor concentration of 1 microM, 91% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-benzyl-5-(dibromoacetyl)benzamide
-
14% inhibition at inhibitor concentration of 100 nM, 27% inhibition at inhibitor concentration of 1 microM, 75% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
3-benzyl-5-(difluoroacetyl)benzamide
-
-
3-carbamoylphenyl furan-2-carboxylate
-
5% inhibition at 0.001 mM
3-chloro-5-(furan-2-ylmethoxy)pyridine
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
3-chlorophenyl furan-2-carboxylate
hepatitis A virus
-
11% inhibition at 10 microM inhibitor concentration
3-phenyl-1-(3,4-dichlorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
3-phenyl-1-(3-chlorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
3-phenyl-1-(3-nitrophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
3-phenyl-1-(4-chlorophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
3-phenyl-1-(4-cyanophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
3-phenyl-1-(4-fluorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
3-phenyl-1-(4-methoxyphenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
3-phenyl-1-(4-trifluoromethoxyphenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
3-[N1-(acetyl-L-leucyl-L-alanyl-L-alanyl)-N2-(o-nitrophenyl-sulfenyl)hydrazino]-N,N-(dimethyl)propanamide
hepatitis A virus
-
IC50: 0.1 mM, time-dependent inactivation of the enzyme due to disulfide bond formation with the active site cysteine thiol
-
3-[N1-(bromoacetyl)-N2-(acetyl-L-leucyl-L-alanyl-L-alanyl)hydrazino]-N,N-(dimethyl)propanamide
hepatitis A virus
-
time-dependent irreversible inactivator
-
3-[N1-(chloroacetyl)-N2-(acetyl-L-leucyl-L-alanyl-L-alanyl)hydrazino]1-N,N-(dimethyl)propanamide
hepatitis A virus
-
time-dependent irreversible inactivator
-
4-(bromoacetyl)isoindolin-1-one
-
-
4-acetylisoindolin-1-one
-
-
4-amino-7-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-7-methyl-5-oxooctanoic acid
-
-
4-bromoisoindolin-1-one
-
-
4-chloromercuribenzenesulfonate
-
strong inhibition at 0.05 mM
4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinoline-6-carbaldehyde
-
95% effect at 0.1 mM
4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinoline-6-carbonitrile
-
50% effect at 0.1 mM
4S-(3-benzo[1,3]dioxol-5-yl-acryloylamino)-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(2H-chromene-3-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(2methyl-5-phenylfuran-3-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(6-bromo-2H-chromene-3-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(6-chloro-2H-chromene-3-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(6-methyl-naphthalene-2-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[(7-bromonaphthalene-2-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)-pent-2-enoic acid ethyl ester
-
-
4S-[(naphthalene-2-carbonyl)amino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid
-
-
4S-[3-(2,5-dibromophenyl)acryloylamino]-5-(2-oxopyrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[3-(3-bromo-4-fluorophenyl)acryloylamino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[3-(3-bromo-4-methyl-phenyl)-acryloylamino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[3-(3-bromophenyl)acryloylamino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid ethyl ester
-
-
4S-[3-(6'-bromo-benzo[1,3]dioxol-5-yl)acryloylamino]-5-(2-oxopyrrolidin-3S-yl)pent-2-enoic acid
-
-
5-(bromoacetyl)-N-2-naphthylisophthalamide
-
5% inhibition at inhibitor concentration of 100 nM, 19% inhibition at inhibitor concentration of 1 microM, 80% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
5-(bromoacetyl)-N-phenylisophthalamide
-
8% inhibition at inhibitor concentration of 100 nM, 27% inhibition at inhibitor concentration of 1 microM, 86% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
5-(dibromoacetyl)-N-2-naphthylisophthalamide
-
-
5-(dibromoacetyl)-N-phenylisophthalamide
-
-
5-acetyl-N-(naphthalen-1-yl)benzene-1,3-dicarboxamide
-
-
5-acetyl-N-phenylbenzene-1,3-dicarboxamide
-
-
5-bromopyridin-3-yl (2E)-3-phenylprop-2-enoate
-
63% inhibition at 0.001 mM
5-bromopyridin-3-yl 3-phenylpropanoate
-
34% inhibition at 0.001 mM
5-bromopyridin-3-yl furan-2-carboxylate
-
most potent inhibitor, 90% inhibition at 0.001 mM
5-bromopyridin-3-yl furan-3-carboxylate
hepatitis A virus
-
inhibition not detected at 10 microM inhibitor concentration, above 90% inhibition at 1 microM inhibitor concentration, 87% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1,3-thiazole-4-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 85% inhibition at 1 microM inhibitor concentration, 37% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1-benzofuran-2-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, above 90% inhibition at 1 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1-benzothiophene-2-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 47% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 1-naphthoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 30% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 1H-benzo[d]imidazole-5-carboxylate
hepatitis A virus
-
82% inhibition at 10 microM inhibitor concentration, 75% inhibition at 1 microM inhibitor concentration, 32% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1H-benzo[d][1,2,3]triazole-5-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 1H-imidazole-4-carboxylate
hepatitis A virus
-
33% inhibition at 10 microM inhibitor concentration, 47% inhibition at 1 microM inhibitor concentration, 34% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1H-indole-2-carboxylate
hepatitis A virus
-
80% inhibition at 10 microM inhibitor concentration, 58% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 1H-indole-3-carboxylate
hepatitis A virus
-
13% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 1H-indole-5-carboxylate
hepatitis A virus
-
87% inhibition at 10 microM inhibitor concentration, 60% inhibition at 1 microM inhibitor concentration, 22% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 1H-pyrazole-3-carboxylate
-
82% inhibition at 0.001 mM
5-chloropyridin-3-yl 1H-pyrazole-4-carboxylate
hepatitis A virus
-
56% inhibition at 10 microM inhibitor concentration, 21% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 2,6-dichloro-5-fluoronicotinate
hepatitis A virus
-
44% inhibition at 10 microM inhibitor concentration, 19% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 2-(diphenylmethyl)-4-ethoxy-1,3-oxazole-5-carboxylate
-
5% inhibition at 0.001 mM
5-chloropyridin-3-yl 2-benzyl-4-ethoxy-1,3-oxazole-5-carboxylate
-
5% inhibition at 0.001 mM
5-chloropyridin-3-yl 2-chlorobenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 67% inhibition at 1 microM inhibitor concentration, 23% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 2-methoxybenzoate
hepatitis A virus
-
85% inhibition at 10 microM inhibitor concentration, 57% inhibition at 1 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 2-methylbenzoate
hepatitis A virus
-
82% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 2-naphthoate
hepatitis A virus
-
23% inhibition at 10 microM inhibitor concentration, 35% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 2-nitrobenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 24% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 2-oxo-2H-chromene-3-carboxylate
hepatitis A virus
-
75% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 3,4-dimethoxybenzoate
hepatitis A virus
-
59% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 3-acetoxybenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 27% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 3-chlorobenzoate
hepatitis A virus
-
29% inhibition at 10 microM inhibitor concentration, 50% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 3-methylbenzoate
hepatitis A virus
-
63% inhibition at 10 microM inhibitor concentration, 32% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 3-methylthiophene-2-carboxylate
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 3-[4-(trifluoromethyl)phenyl]-3H-pyrrole-5-carboxylate
-
40% inhibition at 0.001 mM
5-chloropyridin-3-yl 4-(diethylamino)benzoate
hepatitis A virus
-
64% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 4-(dimethylamino)benzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, above 90% inhibition at 1 microM inhibitor concentration, 20% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 4-(methylamino)benzoate
hepatitis A virus
-
82% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 4-aminobenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 26% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 4-chloro-2-hydroxybenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 59% inhibition at 1 microM inhibitor concentration, 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 4-ethoxy-2-(naphthalen-2-yl)-1,3-oxazole-5-carboxylate
-
34% inhibition at 0.001 mM
5-chloropyridin-3-yl 4-ethoxy-2-[(E)-2-phenylethenyl]-1,3-oxazole-5-carboxylate
-
87% inhibition at 0.001 mM
5-chloropyridin-3-yl 4-fluorobenzoate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 45% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 4-hydroxy-7-(trifluoromethyl)quinoline-3-carboxylate
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 4-methoxybenzoate
hepatitis A virus
-
85% inhibition at 10 microM inhibitor concentration; above 90% inhibition at 10 microM inhibitor concentration, 50% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 4-methylbenzoate
hepatitis A virus
-
77% inhibition at 10 microM inhibitor concentration, 70% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 4-sulfamoylbenzoate
hepatitis A virus
-
64% inhibition at 10 microM inhibitor concentration, 57% inhibition at 1 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-(2-(trifluoromethyl)phenyl)furan-2-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-(2-chloro-5-(trifluoromethyl)phenyl)furan-2-carboxylate
hepatitis A virus
-
76% inhibition at 10 microM inhibitor concentration, 36% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl 5-(2-nitrophenyl)furan-2-carboxylate
hepatitis A virus
-
37% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 5-(3-nitrophenyl)furan-2-carboxylate
hepatitis A virus
-
Inhibition not detected at 10 microM, 1 microM and 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-(4-chloro-2-nitrophenyl)furan-2-carboxylate
hepatitis A virus
-
40% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl 5-(4-chlorophenyl)furan-2-carboxylate
5-chloropyridin-3-yl 5-(4-methylphenyl)furan-2-carboxylate
-
79% inhibition at 0.001 mM
5-chloropyridin-3-yl 5-(4-nitrophenyl)furan-2-carboxylate
-
60% inhibition at 0.001 mM
5-chloropyridin-3-yl 5-bromofuran-2-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 54% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-methyl-3-phenylisoxazole-4-carboxylate
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-methylthiophene-2-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 24% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-nitro-1H-pyrazole-3-carboxylate
hepatitis A virus
-
54% inhibition at 10 microM inhibitor concentration, 69% inhibition at 1 microM inhibitor concentration, 53% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl 5-phenylfuran-2-carboxylate
-
80% inhibition at 0.001 mM
5-chloropyridin-3-yl benzoate
5-chloropyridin-3-yl benzo[d]thiazole-6-carboxylate
hepatitis A virus
-
74% inhibition at 10 microM inhibitor concentration, 54% inhibition at 1 microM inhibitor concentration, 27% inhibition at 0.25 microM inhibitor concentration
5-chloropyridin-3-yl benzo[d][1,3]dioxole-5-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 11% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl biphenyl-4-carboxylate
hepatitis A virus
-
39% inhibition at 10 microM inhibitor concentration, 23% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl cinnamate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration, 53% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl cyclohexanecarboxylate
hepatitis A virus
-
81% inhibition at 10 microM inhibitor concentration, 32% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl furan-2-carboxylate
5-chloropyridin-3-yl furan-3-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl isonicotinate
hepatitis A virus
-
37% inhibition at 10 microM inhibitor concentration, 34% inhibition at 1 microM inhibitor concentration
5-chloropyridin-3-yl naphthalene-1-carboxylate
-
75% inhibition at 0.001 mM
5-chloropyridin-3-yl naphthalene-2-carboxylate
-
80% inhibition at 0.001 mM
5-chloropyridin-3-yl pyrazine-2-carboxylate
hepatitis A virus
-
22% inhibition at 10 microM inhibitor concentration
5-chloropyridin-3-yl thiophene-2-carboxylate
5-methylpyridin-2-yl thiophene-2-carboxylate
hepatitis A virus
-
12% inhibition at 10 microM inhibitor concentration
6-(bromoacetyl)isoindolyn-1-one
-
0% inhibition at inhibitor concentration of 100 nM, 25% inhibition at inhibitor concentration of 1 microM, 70% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
6-acetyl-2-(pyridin-3-yl)quinolin-4(1H)-one
-
25% effect at 0.1 mM
6-acetylisoindolin1-one
-
-
6-bromoisoindolin-1-one
-
-
6-chloropyridin-2-yl thiophene-2-carboxylate
hepatitis A virus
-
16% inhibition at 10 microM inhibitor concentration
6-chloropyridin-3-yl 1H-pyrrole-2-carboxylate
hepatitis A virus
-
92% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 1 microM inhibitor concentration, below 10% inhibition at 0.25 microM inhibitor concentration
6-chloropyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
25% inhibition at 10 microM inhibitor concentration
6-methyl-2-nitropyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
14% inhibition at 10 microM inhibitor concentration
6-methylpyridin-2-yl thiophene-2-carboxylate
hepatitis A virus
-
91% inhibition at 10 microM inhibitor concentration, below 10% inhibition at 1 microM inhibitor concentration
6-methylpyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
23% inhibition at 10 microM inhibitor concentration
7-(2-phenylethyl)-2-(pyridin-3-yl)quinolin-4(1H)-one
-
100% effect at 0.1 mM
7-methoxy-2-(pyridin-3-yl)quinolin-4(1H)-one
-
below 10% effect at 0.1 mM
7-methyl-2-(pyridin-3-yl)quinolin-4(1H)-one
-
40% effect at 0.1 mM
7-[hydroxy(phenyl)methyl]-2-(pyridin-3-yl)quinolin-4(1H)-one
-
80% effect at 0.1 mM
acetyl-Ala-Ala-Ala-(N,N'-dimethylglutaminal)
hepatitis A virus
-
-
acetyl-LEALFQ-ethylpropionate
-
-
acetyl-Leu-Ala-Ala N,N-dimethylglutamine fluoroketone
-
irreversible inactivator
acetyl-Leu-Ala-Ala-(N,N'-dimethylglutaminal)
hepatitis A virus
-
-
AG7088
AL21-01
-
Agouron aldehydic compound, 8% inhibition at inhibitor concentration of 100 nM, 75% inhibition at inhibitor concentration of 1 microM, 92% inhibition at inhibitor concentration of 0.01 mM, 700 nM enzyme
-
ammonium trichloro (dioxoethylene-O,O')tellurate
-
-
Aprotinin
-
0.1 mM, 64% inhibition
azidicarboxamide
-
potent, irreversible inhibitors with IC50 values in the low micromolar range, probably act by adding the active site thiol to the azo moiety in a Michael fashion to give a covalent complex
-
benzyloxycarbonyl-Leu-Phe-(glutaminol)
-
-
benzyloxycarbonyl-Leu-Phe-L-(cyanomethyl-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N,N-dimethyl-glutaminyl)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-acetylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-benzoylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-butyloxycarbonylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-carbomethoxyamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-formylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-isobutyrylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-(N-propinylamino-alaninal)
-
-
benzyloxycarbonyl-Leu-Phe-L-glutaminal-hemiaminal
-
-
benzyloxycarbonyl-Leu-Phe-L-[(N-trifluoroacetyl)amino-alaninal]
-
-
benzyloxycarbonyl-Leu-Phe-L-[methional sulfoxide]
-
-
benzyloxycarbonyl-Leu-Phe-L-[N-(2-pyrrolidinone)-alaninal]
-
-
benzyloxycarbonyl-Leu-Phe-L-[N-(isoxazole-5-carbonyl)aminoalaninal]
-
-
benzyloxycarbonyl-Leu-Phe-L-[N-(N,N-dimethylcarbamoyl)amino-alaninal]
-
-
benzyloxycarbonyl-Leu-Phe-[(N-methylsulfonyl)amino-alaninal]
-
-
benzyloxycarbonyl-Leu-Phe-[N-(Me)Ac-amino-alaninal]
-
-
beta-lactones
hepatitis A virus
-
-
-
bis-vinylic organotellurane
-
-
calpastatin
-
0.8 mM, 30% inhibition
-
chloro-telluroxetane
-
-
chymostatin
Cu2+
-
5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added
dimethyl 5-acetylbenzene-1,3-dicarboxylate
-
-
Elastinal
-
-
ethyl (2E,4S)-4-([(2S)-2-[3-[[(5-methyl-1,2-oxazol-3-yl)carbonyl]amino]-2-oxopyridin-1(2H)-yl]pent-4-ynoyl]amino)-5-[(3R)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
compound AG7404
ethyl (2E,4S)-4-([N-[(benzyloxy)carbonyl]-L-phenylalanyl]amino)-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
-
ethyl (2E,4S)-4-[(N-[5-[(tert-butoxycarbonyl)amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl]-L-phenylalanyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
-
ethyl (2E,4S)-4-[(N6-acetyllysyl)amino]-7-amino-7-oxohept-2-enoate
-
-
ethyl (2E,4S)-4-[(tert-butoxycarbonyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
-
ethyl (2E,4S)-4-[[N-(5-amino-6-hydroxy-2-methyl-4-oxohexan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
most potent inhibitor
ethyl (2E,4S)-4-[[N-(5-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
-
ethyl (2E,4S)-4-[[N-(tert-butoxycarbonyl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
-
ethyl (2Z)-3-[4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinolin-6-yl]prop-2-enoate
-
90% effect at 0.1 mM
ethyl 3-amino-5-[1-(hydroxyamino)ethyl]benzoate
-
-
ethyl 3-[4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinolin-6-yl]propanoate
-
0% effect at 0.1 mM
ethyl 4-[2-(4-cinnamoyl)amino-1-oxo-3-phenyl]propylamino-5-(2-oxo-3-pyrrolidyl)-2-pentenoate
-
-
ethyl 4-[2-(tert-butoxycarbonyl)amino-1-oxo-3-phenyl]propylamino-5-(2-oxo-3-pyrrolidyl)-2-pentenoate
-
-
ethyl 4-[2-[3,4-(methylenedioxy)cinnamoyl]amino-1-oxo-3-phenyl]propylamino-5-(2-oxo-3-pyrrolidyl)-2-pentenoate
-
-
ethyl 4-[2-[4-(dimethylamino)cinnamoyl]amino-1-oxo-3-phenyl]propyl-amino-5-(2-oxo-3-pyrrolidyl)-2-pentenoate
-
-
eukaryotic release factor 3
-
increasing concentrations of recombinant His-tagged eRF3 lead to partial inhibition of 3Cpro-proteolytic cleavage of poly(A) binding protein that increases modestly
-
furan-2-yl pyridine-3-carboxylate
hepatitis A virus
-
above 90% inhibition at 10 microM inhibitor concentration
iodoacetamide
iodoacetic acid
-
0.4 mM, complete inhibition
LY338387
-
0.00022 mM, 19% inhibition
LY355455
-
0.00031 mM, 72% inhibition
LY362270
-
0.01 mM, 43% inhibition
methyl 3-benzamido-5-{2,2-difluoro-1-[(2-methoxyethoxy)methoxy]ethenyl}benzoate
-
-
-
methyl 3-benzyl-5-bromobenzoate
-
-
methyl 3-bromo-5-(5,8-dihydronaphthalen-2-ylmethyl)benzoate
-
-
methyl 3-bromo-5-{2,2-difluoro-1-[(2-methoxyethoxy)methoxy]ethenyl}benzoate
-
-
methyl methanethiosulfonate
-
almost complete inhibition at 0.05 mM
methyl-3-(benzylamino)-5-carbamoylbenzoate
-
-
methyl-3-amino-5-carbamoylbenzoate
-
-
methyl-3-carbamoyl-5-(dibenzylamino)benzoate
-
-
methyl-3-{2,2-difluoro-1-[(2-methoxyethoxy)methoxy]ethenyl}-5-{[(trifluoromethyl)sulfonyl]oxy}benzoate
-
-
-
N-(2-chloropyridin-3-yl)thiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-(5-chloropyridin-2-yl)thiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-(5-fluoropyridin-2-yl)thiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-(6-chloropyridin-3-yl)thiophene-2-carboxamide
hepatitis A virus
-
19% inhibition at 10 microM inhibitor concentration
N-(benzyloxycarbonyl)-D-serine-beta-lactone
hepatitis A virus
-
competitive reversible inhibitor
N-(benzyloxycarbonyl)-L-serine-beta-lactone
hepatitis A virus
-
irreversible, inactivation of the enzyme occurs by nucleophilic attack of the cysteine thiol Cys172 at the beta-position of the oxetanione ring
N-(methylsulfonyl)-L-serine-beta-lactone
hepatitis A virus
-
weak time-dependent inhibition
N-(phenethylsulfonyl)-D-serine-beta-lactone
hepatitis A virus
-
irreversible
N-(phenethylsulfonyl)-L-serine-beta-lactone
hepatitis A virus
-
reversible
N-(trans-beta-styrenesulfonyl)-D-serine-beta-lactone
hepatitis A virus
-
reversible
N-(trans-beta-styrenesulfonyl)-L-serine-beta-lactone
hepatitis A virus
-
reversible
N-acetyl-L-alpha-glutamyl-L-phenylalanyl-L-glutaminyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-leucinamide
i.e. Michael acceptor peptidyl inhibitor (MAPI). Irreversible inhibitor in which the natural polyprotein substrate recognition sequence is linked to a propenyl ethyl ester moiety (X). The catalytic cysteine at position 139 of the enzyme is rapidly modified by the inhibitor
N-acetyl-L-leucyl-alanyl-alanyl-(N,N-dimethyl)-glutamine-(1,4-dioxo-3,4-dihydro-1H-phthalazin-2-yl)methyl ketone
hepatitis A virus
-
-
N-acetyl-L-leucyl-alanyl-alanyl-(N,N-dimethyl)-glutamine-fluoromethyl ketone
hepatitis A virus
-
-
N-acetyl-L-leucyl-phenylalanyl-phenylalanyl-glutamate-fluoromethyl ketone
hepatitis A virus
-
-
N-benzoyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
-
-
N-Cbz-L-serine beta-lactone
hepatitis A virus
-
irreversible inhibitor, associated with His 102 of the enzyme
N-ethylmaleimide
-
almost complete inhibition at 0.05 mM
N-iodoacetyl-Val-Phe-amide
hepatitis A virus
-
irreversible inhibitor, alkylates the active site cysteine 172
N-methyl-3-[4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinolin-7-yl]propanamide
-
45% effect at 0.1 mM
N-methyl-4-oxo-2-(pyridin-3-yl)-1,4-dihydroquinoline-7-carboxamide
-
40% effect at 0.1 mM
N-Methylmaleimide
hepatitis A virus
-
-
N-pyridin-2-ylthiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-pyridin-3-ylthiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-pyridin-3-ylthiophene-2-sulfonamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-pyridin-4-ylthiophene-2-carboxamide
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration
N-[(benzyloxy)carbonyl]glycyl-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-isoleucinamide
-
-
N-[(benzyloxy)carbonyl]glycyl-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide
-
-
N-[(benzyloxy)carbonyl]glycyl-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-norleucinamide
-
-
N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-Nalpha-[(2E)-3-phenylprop-2-enoyl]-L-phenylalaninamide
-
-
N2-(morpholin-4-ylcarbonyl)-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide
-
-
N2-[(benzyloxy)carbonyl]-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide
N2-[(benzyloxy)carbonyl]-N-[(2S)-3,4-dioxo-1-(2-oxopyrrolidin-3-yl)-4-(propan-2-ylamino)butan-2-yl]-L-leucinamide
N6-(1-acetylprolylalanyl)-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]lysinamide
-
-
N6-(N-acetylalanyl)-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]lysinamide
-
-
N6-(N-acetylalanylprolylalanyl)-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]lysinamide
-
-
N6-(prolylalanyl)-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]lysinamide
-
-
Na+
-
the enzyme is strongly inhibited by 200 mM Na+
Nalpha-[(2E)-3-(1,3-benzodioxol-5-yl)prop-2-enoyl]-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
Nalpha-[(2E)-3-(4-chloro-2-fluorophenyl)prop-2-enoyl]-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
Nalpha-[(2E)-3-(4-methylphenyl)prop-2-enoyl]-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
Nalpha-[(2E)-4-(5-methyl-1,2-oxazol-3-yl)but-2-enoyl]-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
Nalpha-[(benzyloxy)carbonyl]-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
Nalpha-{(2E)-3-[4-(dimethylamino)phenyl]prop-2-enoyl}-N-{(2S)-1-oxo-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-L-phenylalaninamide
-
-
PABP-interacting protein 2
-
increasing concentrations of 1-3 microg inhibit cleavage of poly(A) binding protein by 3Cpro in a dose-dependent manner
-
peptide-mimetic monofluoromethyl ketones
hepatitis A virus
-
-
-
phenylmethylsulfonyl fluoride
-
-
PMSF
-
1.7 mM, 32% inhibition
poliovirus-encoded nonstructural polypeptide 2B
-
low but detectable activity
-
poliovirus-encoded nonstructural polypeptide 2BC
-
efficiently blocks 3Cpro activity
-
poliovirus-encoded nonstructural polypeptide 2C
-
efficiently blocks 3Cpro activity, inhibits 3Cpro-catalyzed cleavage of cellular transcription factors at Q-G sites in vitro
-
pyridin-2-yl thiophene-2-carboxylate
hepatitis A virus
-
89% inhibition at 10 microM inhibitor concentration, 8% inhibition at 1 microM inhibitor concentration
pyridin-3-yl furan-2-carboxylate
-
8% inhibition at 0.001 mM
pyridin-3-yl thiophene-2-carboxylate
hepatitis A virus
-
83% inhibition at 10 microM inhibitor concentration, 21% inhibition at 1 microM inhibitor concentration
pyridin-4-yl thiophene-2-carboxylate
hepatitis A virus
-
below 10% inhibition at 10 microM inhibitor concentration, 10% inhibition at 1 microM inhibitor concentration
rupintrivir
TLCK
-
0.2 mM, 51% inhibition
Tos-Phe-CH2-Cl
hepatitis A virus
-
-
vinyl sulfones
hepatitis A virus
-
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
activates
Sodium citrate
-
the peptidase activity of picornain-3C is 100fold higher in the presence of 1.5 M sodium citrate
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0431
(2-(N-methylamino)benzoyl)-IEALFQGPPK-(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30C
-
0.00018
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate
hepatitis A virus
-
-
0.0735
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30C
-
0.075
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30C
-
0.0667
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30C
-
0.0482
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30C
-
0.0924
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30C
-
0.0582
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30C
-
0.075
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met
in 50 mM Tris, pH 7.0, at 30C
-
0.0182 - 0.02
2-aminobenzoyl-AALFQGPLQ-EDDnp
0.0264 - 0.0349
2-aminobenzoyl-EAAFQGPLQ-EDDnp
0.0413 - 0.0435
2-aminobenzoyl-EALAQGPLQ-EDDnp
0.0393
2-aminobenzoyl-EALFQAPLQ-EDDnp
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.0533
2-aminobenzoyl-EALFQGALQ-EDDnp
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.0185 - 0.0213
2-aminobenzoyl-EALFQGPAQ-EDDnp
0.014 - 0.0203
2-aminobenzoyl-EALFQGPLQ-EDDnp
0.0058
4-(4-dimethylaminophenylazo)benzoic acid-KTSAVLQSGFRKME-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
-
0.00012
5-bromopyridin-3-yl furan-3-carboxylate
hepatitis A virus
-
-
0.00024
5-chloropyridin-3-yl furan-2-carboxylate
hepatitis A virus
-
-
0.8
Ac-DEFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.3
Ac-EFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
1.5
Ac-FQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
1.3
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu
-
-
0.0632
Dabcyl-IEALFQGPPKFRE-Edans
in 50 mM Tris, pH 7.0, at 30C
-
0.0825
Dabcyl-RTATVQGPSLDFKE-Edans
in 50 mM Tris, pH 7.0, at 30C
-
2.33
N-acetyl-DEFQLQ-4-nitroanilide
human norovirus
-
pH and temperature not specified in the publication
-
0.25 - 2.6
peptides derived from polyprotein cleavage sites
-
-
-
0.099 - 0.391
SLEVLFQGPVRR
0.037 - 0.087
TENLYFQSGTRR
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0085
(2-(N-methylamino)benzoyl)-IEALFQGPPK-(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.00025
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate
hepatitis A virus
-
-
0.0005
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.0027
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.005
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.004
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.003
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.0035
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.0003
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.01 - 0.96
2-aminobenzoyl-AALFQGPLQ-EDDnp
0.08 - 3.34
2-aminobenzoyl-EAAFQGPLQ-EDDnp
0.01 - 0.66
2-aminobenzoyl-EALAQGPLQ-EDDnp
0.34
2-aminobenzoyl-EALFQAPLQ-EDDnp
Enterovirus C
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.28
2-aminobenzoyl-EALFQGALQ-EDDnp
Enterovirus C
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.03 - 2.72
2-aminobenzoyl-EALFQGPAQ-EDDnp
0.06 - 2.02
2-aminobenzoyl-EALFQGPLQ-EDDnp
27
4-(4-dimethylaminophenylazo)benzoic acid-KTSAVLQSGFRKME-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
enterovirus
-
-
0.00119
5-bromopyridin-3-yl furan-3-carboxylate
hepatitis A virus
-
-
0.0011
5-chloropyridin-3-yl furan-2-carboxylate
hepatitis A virus
-
-
0.33
Ac-DEFQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
0.14
Ac-EFQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
0.08
Ac-FQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
0.0028
Dabcyl-IEALFQGPPKFRE-Edans
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.002
Dabcyl-RTATVQGPSLDFKE-Edans
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
-
0.08
N-acetyl-DEFQLQ-4-nitroanilide
human norovirus
-
pH and temperature not specified in the publication
-
0.41 - 2.38
SLEVLFQGPVRR
0.036 - 0.22
TENLYFQSGTRR
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.97
(2-(N-methylamino)benzoyl)-IEALFQGPPK-(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202450
0.007
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202455
0.036
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202457
0.075
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202459
0.083
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202458
0.037
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202454
0.06
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202452
0.0045
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202453
0.41
Ac-DEFQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
41945
0.46
Ac-EFQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
41944
0.05
Ac-FQLQ-4-nitroanilide
Southampton virus
Q04544
pH 8.5, temperature not specified in the publication
41943
0.045
Dabcyl-IEALFQGPPKFRE-Edans
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202456
0.024
Dabcyl-RTATVQGPSLDFKE-Edans
Enterovirus A71
E7E815
in 50 mM Tris, pH 7.0, at 30C
202451
0.034
N-acetyl-DEFQLQ-4-nitroanilide
human norovirus
-
pH and temperature not specified in the publication
202462
4.14 - 6.09
SLEVLFQGPVRR
0.41 - 6.95
TENLYFQSGTRR
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000042
acetyl-Leu-Ala-Ala-(N,N'-dimethylglutaminal)
hepatitis A virus
-
25C, mutant enzyme C24S
0.00004
benzyloxycarbonyl-Leu-Phe-(glutaminol)
-
-
0.00019
benzyloxycarbonyl-Leu-Phe-L-(cyanomethyl-alaninal)
-
-
0.000005
benzyloxycarbonyl-Leu-Phe-L-(N,N-dimethyl-glutaminyl)
-
-
0.000006
benzyloxycarbonyl-Leu-Phe-L-(N-acetylamino-alaninal)
-
-
0.000012
benzyloxycarbonyl-Leu-Phe-L-(N-benzoylamino-alaninal)
-
-
0.000066
benzyloxycarbonyl-Leu-Phe-L-(N-butyloxycarbonylamino-alaninal)
-
-
0.000132
benzyloxycarbonyl-Leu-Phe-L-(N-carbomethoxyamino-alaninal)
-
-
0.000073
benzyloxycarbonyl-Leu-Phe-L-(N-formylamino-alaninal)
-
-
0.000018
benzyloxycarbonyl-Leu-Phe-L-(N-isobutyrylamino-alaninal)
-
-
0.000007
benzyloxycarbonyl-Leu-Phe-L-(N-propinylamino-alaninal)
-
-
0.0036
benzyloxycarbonyl-Leu-Phe-L-glutaminal-hemiaminal
-
-
0.000146
benzyloxycarbonyl-Leu-Phe-L-[(N-trifluoroacetyl)amino-alaninal]
-
-
0.000005
benzyloxycarbonyl-Leu-Phe-L-[methional sulfoxide]
-
-
0.000052
benzyloxycarbonyl-Leu-Phe-L-[N-(2-pyrrolidinone)-alaninal]
-
-
0.000005
benzyloxycarbonyl-Leu-Phe-L-[N-(isoxazole-5-carbonyl)aminoalaninal]
-
-
0.00001
benzyloxycarbonyl-Leu-Phe-L-[N-(N,N-dimethylcarbamoyl)amino-alaninal]
-
-
0.00064
benzyloxycarbonyl-Leu-Phe-[(N-methylsulfonyl)amino-alaninal]
-
-
0.000469
benzyloxycarbonyl-Leu-Phe-[N-(Me)Ac-amino-alaninal]
-
-
0.0015
N-(benzyloxycarbonyl)-D-serine-beta-lactone
hepatitis A virus
-
pH 7.5, 25C
0.184
N-(benzyloxycarbonyl)-L-serine-beta-lactone
hepatitis A virus
-
pH 7.5, 25C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0002 - 0.00177
(2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid
0.075
(3S)-[N3-(benzyloxycarbonyl)-N1-[3'-(N,N-dimethylamino)-3'-oxopropyl]-N1-(p-methylphenylsulfonyl)]-1,3-diaminobutan-2-one
hepatitis A virus
-
weak competitive, IC50: 0.075 mM
0.00014
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate
hepatitis A virus
-
0.1 M potassium phosphate, 2 mM EDTA, pH 7.5, 100 nM proteinase, 37C
0.018 - 0.051
1,3-diphenyl-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.0077
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-(benzyloxy)-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0098
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-3-(1H-imidazol-3-ium-2-yl)-L-alaninamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0027
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-5-ammonio-L-norvalinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.00084
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-N5-[amino(iminio)methyl]-L-ornithinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0012
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-N6-[amino(iminio)methyl]-L-lysinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
2.8
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]glycinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.066
1-acetyl-L-prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-[methoxy(methyl)amino]-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.053
1-acetyl-L-prolyl-L-alanyl-N1-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-glutamamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.13
1-acetyl-L-prolyl-L-alanyl-N6-acetyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-lysinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.021
1-acetyl-L-prolyl-N-[(2S)-1-[[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]amino]-4-ammonio-1-oxobutan-2-yl]-L-alaninamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0038
1-acetyl-L-prolyl-N-[(2S)-1-[[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]amino]-4-[[amino(iminio)methyl]amino]-1-oxobutan-2-yl]-L-alaninamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.00043
1-benzoylprolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0015
1-benzylprolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0016
1-[(4-methylphenyl)sulfonyl]prolyl-L-alanyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-6-ammonio-L-norleucinamide
Foot-and-mouth disease virus
-
at pH 7.4 and 37C
0.0002
2-oxo-1,2-dihydroquinolin-4-yl furan-2-carboxylate
Human rhinovirus sp.
-
in 50 mM Tris (pH 7.6), 1 mM EDTA, at 30C
0.024 - 0.125
3-phenyl-1-(3,4-dichlorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.01 - 0.017
3-phenyl-1-(3-chlorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.0084 - 0.0096
3-phenyl-1-(3-nitrophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
0.013 - 0.025
3-phenyl-1-(4-chlorophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
0.005 - 0.02
3-phenyl-1-(4-cyanophenyl)-4-(4-carboxybenzylidene)-pyrazol-5(4H)-one
0.0068 - 0.022
3-phenyl-1-(4-fluorophenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.012 - 0.0166
3-phenyl-1-(4-methoxyphenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.042 - 0.098
3-phenyl-1-(4-trifluoromethoxyphenyl)-4-(4-carboxybenzylidene)pyrazol-5(4H)-one
0.1
3-[N1-(acetyl-L-leucyl-L-alanyl-L-alanyl)-N2-(o-nitrophenyl-sulfenyl)hydrazino]-N,N-(dimethyl)propanamide
hepatitis A virus
-
IC50: 0.1 mM, time-dependent inactivation of the enzyme due to disulfide bond formation with the active site cysteine thiol
-
0.00008
5-bromopyridin-3-yl furan-2-carboxylate
Human rhinovirus sp.
-
in 50 mM Tris (pH 7.6), 1 mM EDTA, at 30C
0.000053