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Information on EC 3.4.19.12 - ubiquitinyl hydrolase 1 and Organism(s) Gallus gallus

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1
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This record set is specific for:
Gallus gallus
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The taxonomic range for the selected organisms is: Gallus gallus
The enzyme appears in selected viruses and cellular organisms
Synonyms
uch-l1, uchl1, ataxin-3, deubiquitinating enzyme, pgp 9.5, usp22, pgp9.5, plpro, otub1, isopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AD-019
-
-
-
-
CGI-70
-
-
-
-
esterase, ubiquitin thiol-
-
-
-
-
hydrolase, ubiquitin carboxyl-terminal
-
-
-
-
hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch)
-
-
-
-
isopeptidase
-
-
-
-
isopeptidase T
-
-
-
-
Neuron cytoplasmic protein 9.5
-
-
-
-
PGP 9.5
-
-
-
-
PGP9.5
-
-
-
-
ubiquitin C-terminal hydrolase
-
-
-
-
ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch)
-
-
-
-
ubiquitin C-terminal hydrolase 8
-
-
Ubiquitin C-terminal hydrolase UCH37
-
-
-
-
ubiquitin carboxy-terminal hydrolase
-
-
-
-
ubiquitin carboxyl-terminal hydrolase
-
-
-
-
Ubiquitin thiolesterase
-
-
-
-
Ubiquitin thiolesterase L1
-
-
-
-
Ubiquitin thiolesterase L3
-
-
-
-
Ubiquitin thiolesterase L4
-
-
-
-
Ubiquitin thiolesterase L5
-
-
-
-
ubiquitin-carboxyl-terminal hydrolase PGP-9.5
-
-
-
-
UCH-1
-
-
-
-
UCH-8
-
-
UCH-L1
-
-
-
-
UCH-L2
-
-
-
-
UCH-L3
-
-
-
-
UCH-L4
-
-
-
-
UCH-L5
-
-
-
-
yeast ubiquitin hydrolase
-
-
-
-
YUH1
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
189642-63-5
-
86480-67-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
branched polyubiquitin + H2O
ubiquitin
show the reaction diagram
-
substrate is ligated to proteins via epsilon-NH-isopeptide bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
show the reaction diagram
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
poly-His tagged di-ubiquitin + H2O
ubiquitin + ?
show the reaction diagram
-
-
-
-
?
ubiquitin-alpha-NH-dihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
show the reaction diagram
-
-
-
-
?
ubiquitin-alpha-NH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
show the reaction diagram
-
-
-
?
ubiquitin-alphaNH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
show the reaction diagram
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
show the reaction diagram
ubiquitinyldihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
show the reaction diagram
-
UCH-1
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
divalent cations
-
most divalent cations, except Mg2+ and Ca2+ are inhibitory
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
59% inhibition at 5 mM
iodoacetamide
KCl
-
92% inhibition at 0.2 M
Mg2+
-
61% inhibition at 5 mM
Mn2+
-
98% inhibition at 5 mM
N-ethylmaleimide
-
UCH-8
Ubiquitin aldehyde
-
UCH-8
-
ubiquitin-aldehyde
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
poly-L-Arg
-
activation
poly-L-Lys
-
activation
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
UCH-8
7.8
-
assay at
8 - 8.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
-
inactive above and below
7 - 9
-
little or no activity below pH 7.0 and above pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
pectoralis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
UBP28_CHICK
1047
0
118747
Swiss-Prot
other Location (Reliability: 1)
DESI2_CHICK
193
0
21409
Swiss-Prot
other Location (Reliability: 3)
UBP2_CHICK
357
0
40932
Swiss-Prot
Mitochondrion (Reliability: 3)
BAP1_CHICK
700
0
77704
Swiss-Prot
other Location (Reliability: 5)
UBP7_CHICK
1101
0
128297
Swiss-Prot
other Location (Reliability: 2)
ATX3_CHICK
363
0
41608
Swiss-Prot
other Location (Reliability: 2)
OTU1_CHICK
302
0
33384
Swiss-Prot
Mitochondrion (Reliability: 2)
UBP37_CHICK
986
0
110863
Swiss-Prot
other Location (Reliability: 4)
UBP47_CHICK
1375
0
157247
Swiss-Prot
other Location (Reliability: 2)
UBP48_CHICK
1033
1
118691
Swiss-Prot
other Location (Reliability: 2)
OTU6B_CHICK
302
0
34542
Swiss-Prot
other Location (Reliability: 1)
UBP10_CHICK
785
0
86556
Swiss-Prot
other Location (Reliability: 1)
UBP13_CHICK
862
0
96649
Swiss-Prot
other Location (Reliability: 2)
A0A3Q2U9J3_CHICK
933
0
104381
TrEMBL
other Location (Reliability: 3)
A0A1D5P9H5_CHICK
981
0
112101
TrEMBL
other Location (Reliability: 1)
F1P541_CHICK
331
0
37833
TrEMBL
other Location (Reliability: 1)
E1BXQ0_CHICK
424
0
48793
TrEMBL
other Location (Reliability: 3)
A0A3Q2TYU5_CHICK
3553
0
405025
TrEMBL
other Location (Reliability: 3)
A0A1D5PTS9_CHICK
525
0
60127
TrEMBL
other Location (Reliability: 2)
A0A1D5PJS0_CHICK
510
1
57812
TrEMBL
Mitochondrion (Reliability: 4)
A0A1D5PXU2_CHICK
381
0
44082
TrEMBL
other Location (Reliability: 3)
A0A1D5PST6_CHICK
490
1
55357
TrEMBL
Mitochondrion (Reliability: 5)
Q5ZJE0_CHICK
794
0
88332
TrEMBL
other Location (Reliability: 5)
A0A3Q2U5D0_CHICK
756
0
84162
TrEMBL
other Location (Reliability: 1)
A0A1L1RPT9_CHICK
445
0
50238
TrEMBL
other Location (Reliability: 2)
R4GJH4_CHICK
1376
0
157267
TrEMBL
other Location (Reliability: 1)
A0A1D5PPD3_CHICK
1102
0
128608
TrEMBL
other Location (Reliability: 2)
A0A3Q2TX44_CHICK
3600
0
410522
TrEMBL
other Location (Reliability: 3)
A0A1D5PL72_CHICK
1216
0
134382
TrEMBL
Mitochondrion (Reliability: 5)
Q9PW67_CHICK
230
0
26315
TrEMBL
other Location (Reliability: 2)
E1C8W4_CHICK
832
0
93228
TrEMBL
other Location (Reliability: 2)
A0A3Q2TXG9_CHICK
913
0
101289
TrEMBL
other Location (Reliability: 2)
Q9YHZ2_CHICK
403
0
46243
TrEMBL
Mitochondrion (Reliability: 2)
F1NVF5_CHICK
751
0
83305
TrEMBL
other Location (Reliability: 5)
A0A1D5PU96_CHICK
785
0
86415
TrEMBL
other Location (Reliability: 1)
A0A1D5P1U8_CHICK
1033
1
118763
TrEMBL
other Location (Reliability: 2)
A0A1D5P1U9_CHICK
953
0
109066
TrEMBL
other Location (Reliability: 1)
Q9YHZ0_CHICK
587
0
65901
TrEMBL
other Location (Reliability: 2)
E1C214_CHICK
818
0
93134
TrEMBL
other Location (Reliability: 2)
A0A1D5P867_CHICK
700
0
77754
TrEMBL
other Location (Reliability: 5)
A0A3Q2U1T6_CHICK
716
0
79086
TrEMBL
other Location (Reliability: 1)
Q5ZKD8_CHICK
964
0
107469
TrEMBL
other Location (Reliability: 2)
A0A1D5NTA0_CHICK
270
0
31176
TrEMBL
other Location (Reliability: 1)
A0A1D5PFX4_CHICK
1108
0
122689
TrEMBL
other Location (Reliability: 1)
E1BWB2_CHICK
902
0
101849
TrEMBL
other Location (Reliability: 1)
A0A1L1RX67_CHICK
526
0
58915
TrEMBL
other Location (Reliability: 2)
F1P4C5_CHICK
714
0
81604
TrEMBL
other Location (Reliability: 2)
A1IMF0_CHICK
224
0
25111
TrEMBL
other Location (Reliability: 2)
E1BWJ5_CHICK
2552
0
290161
TrEMBL
other Location (Reliability: 2)
E1C3M0_CHICK
815
0
90631
TrEMBL
other Location (Reliability: 2)
Q5ZKF1_CHICK
297
0
33763
TrEMBL
other Location (Reliability: 1)
A0A1D5P643_CHICK
203
0
23420
TrEMBL
other Location (Reliability: 2)
A0A1D5PVZ1_CHICK
702
0
80557
TrEMBL
other Location (Reliability: 4)
A0A1D5P2U8_CHICK
806
0
91785
TrEMBL
other Location (Reliability: 3)
E1BZ59_CHICK
788
0
89657
TrEMBL
other Location (Reliability: 3)
A0A1D5NZF0_CHICK
717
0
79955
TrEMBL
other Location (Reliability: 3)
V9GVG9_CHICK
2552
0
290108
TrEMBL
other Location (Reliability: 2)
E1C900_CHICK
879
0
98046
TrEMBL
other Location (Reliability: 3)
A0A1D5PF19_CHICK
3497
0
399389
TrEMBL
other Location (Reliability: 3)
A0A1D5PG52_CHICK
459
0
50468
TrEMBL
other Location (Reliability: 2)
A0A3Q3AZW0_CHICK
3340
0
380710
TrEMBL
other Location (Reliability: 4)
A0A1D5PEB4_CHICK
994
0
106602
TrEMBL
other Location (Reliability: 2)
A0A1L1RXA3_CHICK
230
0
26289
TrEMBL
other Location (Reliability: 2)
E1C4M6_CHICK
466
0
52671
TrEMBL
Mitochondrion (Reliability: 5)
F1NHV2_CHICK
993
0
110472
TrEMBL
other Location (Reliability: 3)
A0A3Q3AZQ9_CHICK
712
0
81751
TrEMBL
other Location (Reliability: 4)
A0A3Q2U0D7_CHICK
375
0
42982
TrEMBL
other Location (Reliability: 2)
R9PXQ8_CHICK
988
0
111042
TrEMBL
other Location (Reliability: 4)
A0A1D5PDU1_CHICK
708
0
80947
TrEMBL
other Location (Reliability: 1)
A0A452J7X7_CHICK
346
0
38340
TrEMBL
other Location (Reliability: 3)
F6VFQ9_CHICK
543
0
58975
TrEMBL
other Location (Reliability: 1)
A0A3Q2TZC1_CHICK
697
0
80535
TrEMBL
other Location (Reliability: 4)
F1NZV6_CHICK
1673
0
188933
TrEMBL
Mitochondrion (Reliability: 4)
A0A3Q2U4P4_CHICK
787
0
88377
TrEMBL
other Location (Reliability: 2)
F1NPR3_CHICK
794
0
88275
TrEMBL
other Location (Reliability: 5)
A0A1D5P9L9_CHICK
858
0
97053
TrEMBL
other Location (Reliability: 2)
A0A1D5PUL4_CHICK
1614
0
182734
TrEMBL
Mitochondrion (Reliability: 4)
A0A3Q2UAN6_CHICK
1058
1
121172
TrEMBL
other Location (Reliability: 2)
A0A3Q2UDW5_CHICK
997
0
112630
TrEMBL
other Location (Reliability: 3)
H9L017_CHICK
843
0
92525
TrEMBL
other Location (Reliability: 1)
A0A3Q2UMW0_CHICK
439
0
47328
TrEMBL
Mitochondrion (Reliability: 3)
A0A1I7Q407_CHICK
862
0
96663
TrEMBL
other Location (Reliability: 2)
Q5ZI19_CHICK
340
0
39000
TrEMBL
Secretory Pathway (Reliability: 4)
Q9PWC6_CHICK
950
0
108568
TrEMBL
other Location (Reliability: 1)
A0A3Q2UIB8_CHICK
3303
0
376704
TrEMBL
other Location (Reliability: 4)
A0A1L1RZP2_CHICK
377
0
42505
TrEMBL
other Location (Reliability: 3)
A0A3Q3A3M0_CHICK
874
0
97602
TrEMBL
other Location (Reliability: 3)
A0A3Q2UJB4_CHICK
715
0
82050
TrEMBL
other Location (Reliability: 4)
E1BZL0_CHICK
2597
0
292264
TrEMBL
other Location (Reliability: 1)
F1NQJ6_CHICK
520
0
59063
TrEMBL
other Location (Reliability: 1)
A0A1D5P8W0_CHICK
979
0
108393
TrEMBL
other Location (Reliability: 4)
Q5ZIC1_CHICK
1215
0
134346
TrEMBL
Mitochondrion (Reliability: 5)
Q9YHZ1_CHICK
465
0
52426
TrEMBL
other Location (Reliability: 3)
A0A1D5P9F2_CHICK
2612
0
293951
TrEMBL
other Location (Reliability: 1)
F1NSF6_CHICK
3549
0
404841
TrEMBL
other Location (Reliability: 3)
F1NE88_CHICK
963
0
107455
TrEMBL
other Location (Reliability: 2)
R9PXM3_CHICK
1356
0
154849
TrEMBL
other Location (Reliability: 1)
E1BUD6_CHICK
733
0
81327
TrEMBL
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
UCH-4, gel filtration
130000
-
2 * 130000, reducing SDS-PAGE
200000
-
UCH-8, gel filtration
240000
-
UCH-8, gel filtration
35000
45000
-
UCH-3, gel filtration
50000
-
UCH-2, gel filtration
54000
-
UCH-6, UCH-9, UCH-10, gel filtration
810000
-
UCH-7, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 130000, reducing SDS-PAGE
monomer
-
1 * 35000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
UCH-8, to homogeneity
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Woo, S.K.; Baek, S.H.; Lee, J.L.; Yoo, Y.J.; Cho, C.M.; Kang, M.S.; Chung, C.H.
Purification and characterization of a new ubiquitin C-terminal hydrolase (UCH-1) with isopeptidase activity from chick skeletal muscle
J. Biochem.
121
684-689
1997
Gallus gallus
Manually annotated by BRENDA team
Woo, S.K.; Lee, J.I.; Park, I.K.; Yoo, Y.J.; Cho, C.M.; Kang, M.S.; Ha, D.B.; Tanaka, K.; Chung, C.H.
Multiple Ubiquitin C-terminal Hydrolases from Chick Skeletal Muscle
J. Biol. Chem.
270
18766-18773
1995
Gallus gallus
Manually annotated by BRENDA team
Matsui, S.; Sandberg, A.A.; Negoro, S.; Seon, B.K.; Goldstein, G.
Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds
Proc. Natl. Acad. Sci. USA
79
1535-1539
1982
amphibia, Saccharomyces cerevisiae, Gallus gallus, Cricetulus griseus, eukaryota, Mammalia, no activity in Escherichia coli
Manually annotated by BRENDA team
Baek, S.H.; Woo, S.K.; Lee, J.I.; Yoo, Y.J.; Cho, C.M.; Kang, M.S.; Tanaka, K.; Chung, C.H.
New de-ubiquitinating enzyme, ubiquitin C-terminal hydrolase 8, in chick skeletal muscle
Biochem. J.
325
325-330
1997
Gallus gallus
Manually annotated by BRENDA team