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Information on EC 3.4.11.1 - leucyl aminopeptidase and Organism(s) Pseudomonas aeruginosa

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.1 leucyl aminopeptidase
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This record set is specific for:
Pseudomonas aeruginosa
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
Synonyms
leucine aminopeptidase, erap2, leucyl aminopeptidase, leucine amino peptidase, l-leucine aminopeptidase, leucinaminopeptidase, pilsap, leucine aminopeptidase 3, leucylaminopeptidase, adipocyte-derived leucine aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Aminopeptidase A/I
-
-
-
-
cathepsin III
-
-
-
-
cytosol aminopeptidase
-
-
-
-
DR57
-
-
-
-
FTBL proteins
-
-
-
-
L-leucine aminopeptidase
-
-
-
-
leucinamide aminopeptidase
-
-
-
-
leucinaminopeptidase
-
-
-
-
leucine aminopeptidase
-
-
-
-
Leucyl aminopeptidase
-
-
-
-
leucyl peptidase
-
-
-
-
peptidase S
-
-
-
-
proline aminopeptidase
-
-
-
-
Prolyl aminopeptidase
-
-
-
-
proteins, specific or class, FTBL
-
-
-
-
PATHWAY SOURCE
PATHWAYS
CAS REGISTRY NUMBER
COMMENTARY hide
9001-61-0
-
90119-07-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
show the reaction diagram
-
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
show the reaction diagram
-
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
not active on Ala-para-nitranilide
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.5 mM, completely inhibited
1,7-phenanthroline
-
0.5 mM, 93% activity
4,7-phenanthroline
-
0.5 mM, 95% activity
dithiothreitol
-
10 mM, completely inhibited
EDTA
-
10 mM, 60% activity
EGTA
-
10 mM, 48% activity
N-ethylmaleimide
-
5 mM, 95% activity
tetraethylene pentamine
-
1 mM, completely inhibited
additional information
-
not inhibited by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, tosyl-lysine chloromethylketone, N-methylmaleimide, phosphoramidon
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
155.4
-
periplasm, cleavage of leucyl-hydrazide
18.36
-
cytosol, cleavage of leucyl-hydrazide
additional information
-
1.5-4 units/mg, one unit is the amount of enzyme that causes an increase in optical density at 405 nm of 1 unit/h, substrate 0.6 mM leucine-p-nitroanilide in 1 ml of 0.05 M Tris/HCl, 1 mM CaCl2, pH 8.3
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
lack of extracellular PaAP in biofilms leading to bacterial cell death implies that PaAP might function as a nutrient recycler within a biofilm. Biomass reduction of mutant DELTApaaP grown under a continuous media flow has one day delay compared with the DELTApaaP pellicles grown on static media. Lack of extracellular PaAP triggers bacterial cell death and bacterial cell death in DELTApaaP biofilms is independent of Psl, while the disruption of DELTApaaP biofilms is a result of the degradation of Psl matrix by PslG released from the dead bacteria within biofilms, which in turn led to the dispersion of live bacteria from biofilms into effluents
physiological function
the extracellular aminopeptidase PaAP can release amino acids from the N-terminus of peptide or protein. It modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide Psl and bacterial cell death. PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density. PaAP might function as a nutrient recycler within a biofilm
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8G3BSW6_PSEAI
495
0
52374
TrEMBL
-
A0A8G4JXN3_PSEAI
495
0
52362
TrEMBL
-
A0A8G7CKJ5_PSEAI
495
0
52346
TrEMBL
-
A0A3S3UV79_PSEAI
495
0
52362
TrEMBL
-
A0A8G2VID4_PSEAI
495
0
52362
TrEMBL
-
A0A8G2PCA4_PSEAI
495
0
52400
TrEMBL
-
A0A8G4FNY1_PSEAI
495
0
52360
TrEMBL
-
A0A8G6X2U2_PSEAI
495
0
52418
TrEMBL
-
A0A8B4ZJQ5_PSEAI
495
0
52274
TrEMBL
-
A0A8G6DK76_PSEAI
495
0
52360
TrEMBL
-
A0A8G2VWQ0_PSEAI
495
0
52461
TrEMBL
-
A0A8G6Y3G9_PSEAI
495
0
52376
TrEMBL
-
A0A8G5V7J2_PSEAI
495
0
52390
TrEMBL
-
A0A8G7AKY7_PSEAI
495
0
52388
TrEMBL
-
A0A8G4MWQ3_PSEAI
495
0
52330
TrEMBL
-
A0A8G6YSW9_PSEAI
495
0
52374
TrEMBL
-
A0A4P0VDE9_PSEAI
553
0
59425
TrEMBL
-
A0A7M3AVP7_PSEAI
495
0
52376
TrEMBL
-
A0A8G2VNV7_PSEAI
495
0
52334
TrEMBL
-
A0A8G2U648_PSEAI
495
0
52348
TrEMBL
-
A0A8G2TTT6_PSEAI
495
0
52375
TrEMBL
-
A0A431XD16_PSEAI
495
0
52395
TrEMBL
-
A0A8G6WBR6_PSEAI
495
0
52372
TrEMBL
-
A0A4Z0G9N3_PSEAI
495
0
52347
TrEMBL
-
A0A8G7PZ44_PSEAI
495
0
52392
TrEMBL
-
A0A8G4L8Q3_PSEAI
495
0
52328
TrEMBL
-
A0A8F9K093_PSEAI
495
0
52360
TrEMBL
-
A0A080VIF6_PSEAI
495
0
52360
TrEMBL
-
A0A0D6G9Z6_PSEAI
495
0
52332
TrEMBL
-
A0A3M5EKS9_PSEAI
485
0
51368
TrEMBL
-
A0A8G2QZ13_PSEAI
495
0
52318
TrEMBL
-
A0A2R3J340_PSEAI
495
0
52397
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
SDS-PAGE, AP 28
400000
-
gel filtration
56000
-
SDS-PAGE, AP 56
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
conversion to a 28 kDa protein that retained enzyme activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene paaP, cloning in Escherichia coli strain DH5alpha
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
enzyme PaAP is a therapeutic target for preventing Pseudomonas aeruginosa infection and combating biofilm-related complications
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fricke, B.; Aurich, H.
Periplasmic aminopeptidases in Acinetobacter calcoaceticus and Pseudomonas aeruginosa
J. Basic Microbiol.
33
291-299
1993
Acinetobacter calcoaceticus, Pseudomonas aeruginosa, Acinetobacter calcoaceticus 69-V
Manually annotated by BRENDA team
Jensch, T.; Fricke, B.
Localization of alanyl aminopeptidase and leucyl aminopeptidase in cells of Pseudomonas aeruginosa by application of different methods for periplasm release
J. Basic Microbiol.
37
115-128
1997
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Cahan, R.; Axelrad, I.; Safrin, M.; Ohmann, D.E.; Kessler, E.
A secreted aminopeptidase of Pseudomonas aeruginosa
J. Biol. Chem.
276
43645-43652
2001
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Zhao, T.; Zhang, Y.; Wu, H.; Wang, D.; Chen, Y.; Zhu, M.-J.; Ma, L.Z.
Extracellular aminopeptidase modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide and bacterial cell death
Environ. Microbiol. Rep.
10
583-593
2018
Pseudomonas aeruginosa (Q9HZQ8), Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 (Q9HZQ8), Pseudomonas aeruginosa 1C (Q9HZQ8), Pseudomonas aeruginosa PRS 101 (Q9HZQ8), Pseudomonas aeruginosa DSM 22644 (Q9HZQ8), Pseudomonas aeruginosa CIP 104116 (Q9HZQ8), Pseudomonas aeruginosa LMG 12228 (Q9HZQ8), Pseudomonas aeruginosa JCM 14847 (Q9HZQ8)
Manually annotated by BRENDA team