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3,N4-ethenocytosine-mismatched single-stranded DNA + H2O
3,N4-ethenocytosine + single-stranded DNA with abasic site
-
-
-
?
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
5-formyluracil-mismatched single-stranded DNA + H2O
5-formyluracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
dUMP-labeled calf thymus DNA + H2O
uracil + ?
-
-
-
?
fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G + H2O
?
-
-
-
-
?
M6-FAM-labeled single stranded oligonucleotide + H2O
uracil + ?
T12-AGUA-T12
-
-
?
uracil-containing calf thymus DNA + H2O
uracil + calf thymus DNA with abasic site
-
-
-
?
uracil-containing DNA + H2O
uracil + DNA with abasic site
-
uracil-containing DNA substrates with two uracil sites spaced 10, 40 or 80 bp apart
-
-
?
uracil-containing single stranded DNA + H2O
uracil + single stranded DNA with abasic site
-
duplex single stranded DNAs with sequences 5'-TGCACUUAAGAAUUTC-3'/5'-GAAATTCTTAAGTGCAGTGATAGTCTTCCGTCC-(CH2)7-methylene blue-3' and 5'-GAAATTCTTAAGTGCAGTGATAGTCTTCCGTCC-(CH2)7-methylene blue-3'/5'-TGGGGGTGCACTTAAGAATTTC-3'
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched double-stranded DNA with U-A mismatch + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA with U-G mismatch + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
additional information
?
-
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
-
-
-
?
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
-
-
-
-
?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
-
-
-
-
?
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
-
-
-
-
?
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
isoform UNG1, which in contrast to isoform UNG2 lacks a PCNA-binding motif, may be specialized to act on single stranded DNA (ssDNA) through its ability to bind ssDNA-binding protein RPA
-
-
?
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
-
-
-
-
?
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
the enzyme initiates repair of uracil-DNA is achieved in a base-excision pathway
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
substrate recognition by family-1 UDG, no activity against G-T mismatches or any of a range of other possible substrates, modelling, detailed overview
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
uracil detection and nucleotide flipping by UDG, pinchpushpull uracil detection mechanism, DNA binding structure, modelling, overview. The UDG shows preference for U-G mispairs compaired to U-A mispairs
-
-
?
uracil-mismatched double-stranded DNA with U-G mismatch + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA with U-G mismatch + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
single-stranded 26-mer uracil-containing 2'-deoxyribose oligonucleotide
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
SMUG1 is specific for ssDNA substrates, substrate recognition by family-3 SMUG, modelling, detailed overview
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil
-
-
?
additional information
?
-
-
dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil
-
-
?
additional information
?
-
-
family-1 enzymes are active against uracil in ssDNA and dsDNA, and recognise uracil explicitly in an extrahelical conformation via a combination of protein and bound-water interactions. Extrahelical recognition requires an efficient process of substrate location by base-sampling probably by hopping or gliding along the DNA. Family-2 enzymes are mismatch specific and explicitly recognise the widowed guanine on the complementary strand rather than the extrahelical scissile pyrimidine. Although structures are not yet available for family-3/SMUG and family-4 enzymes, sequence analysis suggests similar overall folds, and identifies common active site motifs but with a surprising lack of conservation of catalytic residues between members of the super-family
-
-
?
additional information
?
-
-
hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview
-
-
?
additional information
?
-
hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview
-
-
?
additional information
?
-
hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview
-
-
?
additional information
?
-
-
hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview
-
-
?
additional information
?
-
-
hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview
-
-
?
additional information
?
-
hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview
-
-
?
additional information
?
-
-
UDG initiates DNA base excision repair, BER, by hydrolyzing the uracil base from the deoxyribose. BER repairs a wide range of base lesions through the use of many different DNA glycosylases specific for distinct types of DNA damage, UDG activity is cell-cycle dependent and generally higher in proliferating cells than in non-cycling cells, overview
-
-
?
additional information
?
-
UNG2 is an important enzyme in the base excision repair pathway, interaction with Ugene is involved in the phenotype of colon cancer, Ugene interacts with the base excision repair pathway, overview
-
-
?
additional information
?
-
uracil DNA glycosylase acts in removing uracil from the sugar backbone of DNA, leaving abasic sites and initiating the uracil base-excision-repair pathway, BER. The human UNG2 enzyme, but not UNG1, is packaged and incorporated into HIV-1 virions via specific interaction with the integrase domain of the Gag-Pol precursor, the virally Vpr protein might also able to mediate the incorporation of UNG2, packaged UNG2 can process uracil from DNA, indicating that HIV-1 has the ability to control dUTP misincorporation in viral DNA, the enzyme is essential to the HIV-1 life cycle. HIV-1 RT and UNG2 recombinant proteins can process uracil from primer-template substrate, molecular mechanism
-
-
?
additional information
?
-
preference of hSMUG1 for mispaired uracil over uracil paired with adenine, substrate selectivity with oligonucleotide 24-mers containing uracil with different 5-substituents, overview
-
-
?
additional information
?
-
-
preference of hSMUG1 for mispaired uracil over uracil paired with adenine, substrate selectivity with oligonucleotide 24-mers containing uracil with different 5-substituents, overview
-
-
?
additional information
?
-
SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview
-
-
?
additional information
?
-
-
SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview
-
-
?
additional information
?
-
SMUG1 is an uracil-DNA glycosylase, that also shows xanthine-DNA glycosylase activity, XDG, EC 3.2.2.15
-
-
?
additional information
?
-
-
SMUG1 is an uracil-DNA glycosylase, that also shows xanthine-DNA glycosylase activity, XDG, EC 3.2.2.15
-
-
?
additional information
?
-
specific excision and removal of dUTP from dsDNA and ssDNA, cleavage of for U-A and U-G pairs. UNG2 binds to Ugene, a nuclear protein overexpressed in colon cancer, Ugene-p binds to the NH2-terminus of UNG2, which does not directly alter UNG2 enzymatic activity or localization, interaction analysis, overview
-
-
?
additional information
?
-
substrate specificity of SMUG, activity of hSMUG1 against uracil containing single- and double-stranded DNA containing matched and mismatched uracil, overview
-
-
?
additional information
?
-
-
substrate specificity of SMUG, activity of hSMUG1 against uracil containing single- and double-stranded DNA containing matched and mismatched uracil, overview
-
-
?
additional information
?
-
substrate specificity, overview. hSMUG1 removes damaged bases from Fenton-oxidized calf thymus DNA, generating abasic sites
-
-
?
additional information
?
-
-
substrate specificity, overview. hSMUG1 removes damaged bases from Fenton-oxidized calf thymus DNA, generating abasic sites
-
-
?
additional information
?
-
substrate specificity, the enzyme is not active with other oxidized pyrimidines such as 5-hydroxycytosine, 5-formylcytosine and thymine glycol, and intact pyrimidines such as thymine and cytosine. Mutational analysis of the catalytic and damage-recognition mechanism of hSMUG1, overview
-
-
?
additional information
?
-
-
substrate specificity, the enzyme is not active with other oxidized pyrimidines such as 5-hydroxycytosine, 5-formylcytosine and thymine glycol, and intact pyrimidines such as thymine and cytosine. Mutational analysis of the catalytic and damage-recognition mechanism of hSMUG1, overview
-
-
?
additional information
?
-
-
hSMUG1 shows excision activity for 5-formyluracil, a major thymine lesion formed by ionizing radiation, opposite all normal bases in DNA
-
-
?
additional information
?
-
-
UNG2 of the human host is required by HIV-1 strain R5, but not by X4HIV, during the early stage of infection
-
-
?
additional information
?
-
-
uracil DNA glycosylase does not show any activity on G:IU, i.e. iodouridine, or A:IU mispairs
-
-
?
additional information
?
-
-
uracil in single-stranded DNA, resulting from incorporation of dUMP during replication and from spontaneous or enzymatic deamination of cytosine, causing U:A pairs or U:G mismatches, respectively, has to be removed by the enzyme. Nuclear UNG2 is apparently the sole contributor to the post-replicative repair of U:A lesions and to the removal of uracil from U:G contexts in immunoglobulin genes as part of somatic hypermutation and class-switch recombination processes in adaptive immunity. UNG2 and SMUG1 contribute to U:G repair. UNG2 is highly specific for uracil, SMUG1 also efficiently removes 5-hydroxymethyluracil
-
-
?
additional information
?
-
-
uracil-DNA glycosylases are ubiquitously found enzymes that hydrolyze the N-glycosidic bond of deoxyuridine, generating from deamination of cytosine, in DNA, UNG enzymes specifically excise Ura bases from both double-stranded and single-stranded DNA with a slight preference for the latter substrate, and shows no activity against normal DNA bases or against uracil in RNA. As potentially mutagenic and deleterious for cell regulation, uracil must be removed from DNA
-
-
?
additional information
?
-
-
excision of 5-formyluracil, fU, from fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3'. Substrate-binding pocket of hSMUG1 and its interactions with uracil and fU, base-pairing properties of fU residues in DNA, structures, overview
-
-
?
additional information
?
-
-
SMUG1 binds tightly to AP sites and inhibits cleavage by AP-endonucleases
-
-
?
additional information
?
-
-
UNG hydrolyzes the N-glycosidic bond of deoxyuridine in DNA. It binds with appreciable affinity to any DNA, mainly due to the interactions with the charged backbone. Search for the lesion by UNG involves random sliding along DNA alternating with dissociation-association events and partial eversion of undamaged bases for initial sampling. DNA in the complex with UNG is highly distorted to allow the extrahelical recognition of uracil, mechanism of uracil search and recognition by UNG, structure-function relationship, overview
-
-
?
additional information
?
-
-
the enzyme primarily interacts with the phosphate backbone on the single strand of DNA. The enzyme does not require a continuous polyanion DNA strand
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,N4-ethenocytosine-mismatched single-stranded DNA + H2O
3,N4-ethenocytosine + single-stranded DNA with abasic site
-
-
-
?
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
5-formyluracil-mismatched single-stranded DNA + H2O
5-formyluracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
additional information
?
-
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
-
-
-
?
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
-
-
-
-
?
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
-
-
-
?
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
isoform UNG1, which in contrast to isoform UNG2 lacks a PCNA-binding motif, may be specialized to act on single stranded DNA (ssDNA) through its ability to bind ssDNA-binding protein RPA
-
-
?
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
-
-
-
-
?
uracil-mismatched DNA + H2O
uracil + DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
-
the enzyme initiates repair of uracil-DNA is achieved in a base-excision pathway
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
-
-
-
?
additional information
?
-
dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil
-
-
?
additional information
?
-
-
dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil
-
-
?
additional information
?
-
-
family-1 enzymes are active against uracil in ssDNA and dsDNA, and recognise uracil explicitly in an extrahelical conformation via a combination of protein and bound-water interactions. Extrahelical recognition requires an efficient process of substrate location by base-sampling probably by hopping or gliding along the DNA. Family-2 enzymes are mismatch specific and explicitly recognise the widowed guanine on the complementary strand rather than the extrahelical scissile pyrimidine. Although structures are not yet available for family-3/SMUG and family-4 enzymes, sequence analysis suggests similar overall folds, and identifies common active site motifs but with a surprising lack of conservation of catalytic residues between members of the super-family
-
-
?
additional information
?
-
-
hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview
-
-
?
additional information
?
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hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview
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additional information
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hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview
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additional information
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hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview
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additional information
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hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview
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additional information
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hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview
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additional information
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UDG initiates DNA base excision repair, BER, by hydrolyzing the uracil base from the deoxyribose. BER repairs a wide range of base lesions through the use of many different DNA glycosylases specific for distinct types of DNA damage, UDG activity is cell-cycle dependent and generally higher in proliferating cells than in non-cycling cells, overview
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additional information
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UNG2 is an important enzyme in the base excision repair pathway, interaction with Ugene is involved in the phenotype of colon cancer, Ugene interacts with the base excision repair pathway, overview
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additional information
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uracil DNA glycosylase acts in removing uracil from the sugar backbone of DNA, leaving abasic sites and initiating the uracil base-excision-repair pathway, BER. The human UNG2 enzyme, but not UNG1, is packaged and incorporated into HIV-1 virions via specific interaction with the integrase domain of the Gag-Pol precursor, the virally Vpr protein might also able to mediate the incorporation of UNG2, packaged UNG2 can process uracil from DNA, indicating that HIV-1 has the ability to control dUTP misincorporation in viral DNA, the enzyme is essential to the HIV-1 life cycle. HIV-1 RT and UNG2 recombinant proteins can process uracil from primer-template substrate, molecular mechanism
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additional information
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hSMUG1 shows excision activity for 5-formyluracil, a major thymine lesion formed by ionizing radiation, opposite all normal bases in DNA
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additional information
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UNG2 of the human host is required by HIV-1 strain R5, but not by X4HIV, during the early stage of infection
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additional information
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uracil DNA glycosylase does not show any activity on G:IU, i.e. iodouridine, or A:IU mispairs
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additional information
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uracil in single-stranded DNA, resulting from incorporation of dUMP during replication and from spontaneous or enzymatic deamination of cytosine, causing U:A pairs or U:G mismatches, respectively, has to be removed by the enzyme. Nuclear UNG2 is apparently the sole contributor to the post-replicative repair of U:A lesions and to the removal of uracil from U:G contexts in immunoglobulin genes as part of somatic hypermutation and class-switch recombination processes in adaptive immunity. UNG2 and SMUG1 contribute to U:G repair. UNG2 is highly specific for uracil, SMUG1 also efficiently removes 5-hydroxymethyluracil
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additional information
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uracil-DNA glycosylases are ubiquitously found enzymes that hydrolyze the N-glycosidic bond of deoxyuridine, generating from deamination of cytosine, in DNA, UNG enzymes specifically excise Ura bases from both double-stranded and single-stranded DNA with a slight preference for the latter substrate, and shows no activity against normal DNA bases or against uracil in RNA. As potentially mutagenic and deleterious for cell regulation, uracil must be removed from DNA
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Adenocarcinoma
Differential role of Wnt signaling and base excision repair pathways in gastric adenocarcinoma aggressiveness.
Adenocarcinoma of Lung
Evaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma.
Anemia
Hematopoietic and gastric uracil-DNA glycosylase activity in megaloblastic anemia and in atrophic gastritis with special reference to pernicious anemia.
Anemia, Megaloblastic
Hematopoietic and gastric uracil-DNA glycosylase activity in megaloblastic anemia and in atrophic gastritis with special reference to pernicious anemia.
Anemia, Pernicious
Hematopoietic and gastric uracil-DNA glycosylase activity in megaloblastic anemia and in atrophic gastritis with special reference to pernicious anemia.
Astrocytoma
Expression analyses of 27 DNA repair genes in astrocytoma by TaqMan low-density array.
Blast Crisis
Uracil-DNA glycosylase activity in human acute leukemia.
Blister
Enzymatic repair of premutagenic DNA lesions in human epidermis. Quantitation of O6-methylguanine-DNA methyltransferase and uracil-DNA glycosylase activities.
Bloom Syndrome
Abnormal regulation of uracil-DNA glycosylase induction during cell cycle and cell passage in Bloom's syndrome fibroblasts.
Bloom Syndrome
Increased levels of 5-fluorouracil-induced DNA lesions in Bloom's syndrome.
Bloom Syndrome
Normal uracil-DNA glycosylase activity in Bloom's syndrome cells.
Brain Injuries
Increased postischemic brain injury in mice deficient in uracil-DNA glycosylase.
Breast Neoplasms
Clinicopathological significance of ATM-Chk2 expression in sporadic breast cancers: a comprehensive analysis in large cohorts.
Breast Neoplasms
Genetic variations in 3'UTRs of SMUG1 and NEIL2 genes modulate breast cancer risk, survival and therapy response.
Breast Neoplasms
Single nucleotide polymorphisms in uracil-processing genes, intake of one-carbon nutrients and breast cancer risk.
Breast Neoplasms
Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) deficiency is linked to aggressive breast cancer and predicts response to adjuvant therapy.
Carcinogenesis
Differential role of Wnt signaling and base excision repair pathways in gastric adenocarcinoma aggressiveness.
Carcinogenesis
Repair of premutagenic DNA lesions in human fetal tissues: evidence for low levels of O6-methylguanine-DNA methyltransferase and uracil-DNA glycosylase activity in some tissues.
Carcinoma
Association of SMUG1 SNPs in Intron Region and Linkage Disequilibrium with Occurrence of Cervical Carcinoma and HPV Infection in Chinese Population.
Carcinoma
Significant Contribution of DNA Repair Human 8-Oxoguanine DNA N-Glycosylase 1 Genotypes to Renal Cell Carcinoma.
Carcinoma, Hepatocellular
Comparison at the molecular level of uracil-DNA glycosylases from different origins.
Carcinoma, Hepatocellular
dUTP pyrophosphatase and uracil-DNA glycosylase in rat liver and hepatomas.
Carcinoma, Ovarian Epithelial
Germline whole exome sequencing and large-scale replication identifies FANCM as a likely high grade serous ovarian cancer susceptibility gene.
Carcinoma, Renal Cell
Significant Contribution of DNA Repair Human 8-Oxoguanine DNA N-Glycosylase 1 Genotypes to Renal Cell Carcinoma.
Carcinoma, Squamous Cell
Association of SMUG1 SNPs in Intron Region and Linkage Disequilibrium with Occurrence of Cervical Carcinoma and HPV Infection in Chinese Population.
Cataract
Polymorphisms of the WRN gene and DNA damage of peripheral lymphocytes in age-related cataract in a Han Chinese population.
Colonic Neoplasms
Functional interaction of Ugene and EBV infection mediates tumorigenic effects.
Colonic Neoplasms
Identification of different mutational profiles in cancers arising in specific colon segments by next generation sequencing.
Colonic Neoplasms
Mechanisms for the involvement of DNA methylation in colon carcinogenesis.
Colonic Neoplasms
Uracil-initiated base excision DNA repair synthesis fidelity in human colon adenocarcinoma LoVo and Escherichia coli cell extracts.
Colorectal Neoplasms
Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition.
Colorectal Neoplasms
Genetic variations in 3'UTRs of SMUG1 and NEIL2 genes modulate breast cancer risk, survival and therapy response.
Esophageal Neoplasms
Uracil-DNA glycosylase (UNG) rs246079 G/A polymorphism is associated with decreased risk of esophageal cancer in a Chinese population.
Gastritis, Atrophic
Hematopoietic and gastric uracil-DNA glycosylase activity in megaloblastic anemia and in atrophic gastritis with special reference to pernicious anemia.
Glioblastoma
Fidelity and mutational specificity of uracil-initiated base excision DNA repair synthesis in human glioblastoma cell extracts.
Glioblastoma
Uracil-initiated base excision DNA repair synthesis fidelity in human colon adenocarcinoma LoVo and Escherichia coli cell extracts.
Glioma
DNA synthesis enzymes and proliferating cell nuclear antigen in normal and neoplastic nerve cells.
Graves Disease
The 8-oxoguanine DNA N-glycosylase 1 (hOGG1) Ser326Cys variant affects the susceptibility to Graves' disease.
Hepatitis B
Concerted action of activation-induced cytidine deaminase and uracil-DNA glycosylase reduces covalently closed circular DNA of duck hepatitis B virus.
Hepatitis C
[Utilization of Uracil-DNA glycosylase for combining reverse transcription and anti-contamination with polymerase chain reaction in hepatitis C virus]
Herpes Simplex
A 3' coterminal gene cluster in pseudorabies virus contains herpes simplex virus UL1, UL2, and UL3 gene homologs and a unique UL3.5 open reading frame.
Herpes Simplex
A kinetic analysis of substrate recognition by uracil-DNA glycosylase from herpes simplex virus type 1.
Herpes Simplex
Cloning and expression of the uracil-DNA glycosylase inhibitor (UGI) from bacteriophage PBS-1 and crystallization of a uracil-DNA glycosylase-UGI complex.
Herpes Simplex
Computational rationale for the selective inhibition of the herpes simplex virus type 1 uracil-DNA glycosylase enzyme.
Herpes Simplex
Crystallization and preliminary X-ray analysis of the uracil-DNA glycosylase DNA repair enzyme from herpes simplex virus type 1.
Herpes Simplex
Direct measurement of the substrate preference of uracil-DNA glycosylase.
Herpes Simplex
DNA glycosylases.
Herpes Simplex
Gene UL2 of herpes simplex virus type 1 encodes a uracil-DNA glycosylase.
Herpes Simplex
Herpes simplex virus type 1 (HSV-1) uracil-DNA glycosylase: functional expression in Escherichia coli, biochemical characterization, and selective inhibition by 6-(p-n-octylanilino)uracil.
Herpes Simplex
Herpes simplex virus type 1 uracil-DNA glycosylase: isolation and selective inhibition by novel uracil derivatives.
Herpes Simplex
Identification of the coding sequence for herpes simplex virus uracil-DNA glycosylase.
Herpes Simplex
Induction of uracil-DNA glycosylase and dUTP nucleotidohydrolase activity in herpes simplex virus-infected human cells.
Herpes Simplex
Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase.
Herpes Simplex
Isolation of a herpes simplex virus cDNA encoding the DNA repair enzyme uracil-DNA glycosylase.
Herpes Simplex
Molecular modeling and synthesis of inhibitors of herpes simplex virus type 1 uracil-DNA glycosylase.
Herpes Simplex
Purification and characterization of the herpes simplex virus type 2-encoded uracil-DNA glycosylase.
Herpes Simplex
Selective inhibition of herpes simplex virus type-1 uracil-DNA glycosylase by designed substrate analogs.
Herpes Simplex
The structural basis of specific base-excision repair by uracil-DNA glycosylase.
Herpes Simplex
Uracil in OriS of herpes simplex 1 alters its specific recognition by origin binding protein (OBP): does virus induced uracil-DNA glycosylase play a key role in viral reactivation and replication?
Herpes Simplex
Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.
Herpes Simplex
Use of the PBS2 uracil-DNA glycosylase inhibitor to differentiate the uracil-DNA glycosylase activities encoded by herpes simplex virus types 1 and 2.
Hyper-IgM Immunodeficiency Syndrome
Clinical Features and Genetic Analysis of Taiwanese Patients with the Hyper IgM Syndrome Phenotype.
Hyper-IgM Immunodeficiency Syndrome
Human uracil-DNA glycosylase deficiency associated with profoundly impaired immunoglobulin class-switch recombination.
Hypersensitivity
Depletion of dimeric all-alpha dUTPase induces DNA strand breaks and impairs cell cycle progression in Trypanosoma brucei.
Infections
Characterization of human cytomegalovirus uracil DNA glycosylase (UL114) and its interaction with polymerase processivity factor (UL44).
Infections
Clinical Features and Genetic Analysis of Taiwanese Patients with the Hyper IgM Syndrome Phenotype.
Infections
Early events after infection of Escherichia coli by bacteriophage T5. III. Inhibition of uracil-DNA glycosylase activity.
Infections
Human APOBEC3G Can Restrict Retroviral Infection in Avian Cells and Acts Independently of both UNG and SMUG1.
Infections
Molecular analysis of a large cohort of patients with the hyper immunoglobulin M (IgM) syndrome.
Infections
Uracil DNA Glycosylase 2 negatively regulates HIV-1 LTR transcription.
Laryngeal Neoplasms
The Cys326 allele of the 8-oxoguanine DNA N-glycosylase 1 gene as a risk factor in smoking- and drinking-associated larynx cancer.
Leukemia
The DNA-repair enzyme uracil-DNA glycosylase in the human hematopoietic system.
Leukemia
Uracil DNA-glycosylase. Purification and properties of this enzyme isolated from blast cells of acute myelocytic leukemia patients.
Leukemia
Uracil-DNA glycosylase activity in human acute leukemia.
Leukemia L5178
Excision of uracil from bromodeoxyuridine-substituted and U.V.-irradiated DNA in cultured mouse lymphoma cells.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Uracil-DNA glycosylase activity in human acute leukemia.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Uracil-DNA glycosylase in benign and malignant maturing human hematopoietic cells.
Leukemia, Myeloid
Uracil DNA-glycosylase. Purification and properties of this enzyme isolated from blast cells of acute myelocytic leukemia patients.
Leukemia, Myeloid, Acute
Uracil DNA-glycosylase. Purification and properties of this enzyme isolated from blast cells of acute myelocytic leukemia patients.
Leukemia, Myeloid, Acute
Uracil-DNA glycosylase activity in human acute leukemia.
Liver Neoplasms, Experimental
dUTP pyrophosphatase and uracil-DNA glycosylase in rat liver and hepatomas.
Lung Neoplasms
DNA repair activity for oxidative damage and risk of lung cancer.
Lung Neoplasms
Interactive effect of cigarette smoking with human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) polymorphisms on the risk of lung cancer: a case-control study in Taiwan.
Lung Neoplasms
The human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) DNA repair enzyme and its association with lung cancer risk.
Lung Neoplasms
Uracil-DNA Glycosylase Expression Determines Human Lung Cancer Cell Sensitivity to Pemetrexed.
Lymphoma, B-Cell
Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
Lymphoproliferative Disorders
Uracil-DNA glycosylase activity in chronic lymphoproliferative disorders.
Macular Degeneration
ASSOCIATION BETWEEN POLYMORPHISM OF THE DNA REPAIR SMUG1 AND UNG GENES AND AGE-RELATED MACULAR DEGENERATION.
Macular Degeneration
Association of the DNA repair SMUG1 rs3087404 polymorphism and its interaction with high sensitivity C-reactive protein for age-related macular degeneration in Iranian patients.
Macular Degeneration
Interactions among different genetic loci in age-related macular degeneration.
Mesothelioma
Evaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma.
Mesothelioma, Malignant
Evaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma.
Monkeypox
Substrate Specificity of Homogeneous Monkeypox Virus Uracil-DNA Glycosylase.
Mouth Neoplasms
The joint effect of smoking and hOGG1 genotype on oral cancer in Taiwan.
Myelodysplastic Syndromes
Hematopoietic and gastric uracil-DNA glycosylase activity in megaloblastic anemia and in atrophic gastritis with special reference to pernicious anemia.
Neoplasms
A robust photoluminescence screening assay identifies uracil-DNA glycosylase inhibitors against prostate cancer.
Neoplasms
A simplified assay for O6-methylguanine-DNA methyltransferase activity and its application to human neoplastic and non-neoplastic tissues.
Neoplasms
Adverse prognostic and predictive significance of low DNA-dependent protein kinase catalytic subunit (DNA-PKcs) expression in early-stage breast cancers.
Neoplasms
Association of SMUG1 SNPs in Intron Region and Linkage Disequilibrium with Occurrence of Cervical Carcinoma and HPV Infection in Chinese Population.
Neoplasms
Clinicopathological significance of ATM-Chk2 expression in sporadic breast cancers: a comprehensive analysis in large cohorts.
Neoplasms
Erratum: The uracil-DNA glycosylase UNG protects the fitness of normal and cancer B cells expressing AID.
Neoplasms
Excision Repair-Initiated Enzyme-Assisted Bicyclic Cascade Signal Amplification for Ultrasensitive Detection of Uracil-DNA Glycosylase.
Neoplasms
Genetic polymorphisms of the DNA repair gene UNG are associated with the susceptibility of rheumatoid arthritis.
Neoplasms
Genetic variations in 3'UTRs of SMUG1 and NEIL2 genes modulate breast cancer risk, survival and therapy response.
Neoplasms
Genome-wide expression profile of sporadic gastric cancers with microsatellite instability.
Neoplasms
Germline ablation of SMUG1 DNA glycosylase causes loss of 5-hydroxymethyluracil- and UNG-backup uracil-excision activities and increases cancer predisposition of Ung-/-Msh2-/- mice.
Neoplasms
Increased uracil-DNA glycosylase, AP-DNA binding protein and deoxyribonuclease activities in tumor and SV40-transformed cell lines of human origin.
Neoplasms
Interindividual variation in the activity of O6-methyl guanine-DNA methyltransferase and uracil-DNA glycosylase in human organs.
Neoplasms
Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification.
Neoplasms
Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner.
Neoplasms
Monoclonal B-cell hyperplasia and leukocyte imbalance precede development of B-cell malignancies in uracil-DNA glycosylase deficient mice.
Neoplasms
Next-generation sequencing identifies recurrent copy number variations in invasive breast carcinomas from Ghana.
Neoplasms
Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population.
Neoplasms
Single nucleotide polymorphisms in uracil-processing genes, intake of one-carbon nutrients and breast cancer risk.
Neoplasms
Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) deficiency is linked to aggressive breast cancer and predicts response to adjuvant therapy.
Neoplasms
Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA.
Neoplasms
The Multiple Cellular Roles of SMUG1 in Genome Maintenance and Cancer.
Neoplasms
The role of deoxyuridine triphosphate nucleotidohydrolase, uracil-DNA glycosylase, and DNA polymerase alpha in the metabolism of FUdR in human tumor cells.
Neoplasms
The uracil-DNA glycosylase UNG protects the fitness of normal and cancer B cells expressing AID.
Neoplasms
UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation.
Neoplasms
Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1.
Neuroblastoma
DNA synthesis enzymes and proliferating cell nuclear antigen in normal and neoplastic nerve cells.
Osteosarcoma
E2F-1 and a cyclin-like DNA repair enzyme, uracil-DNA glycosylase, provide evidence for an autoregulatory mechanism for transcription.
Ovarian Neoplasms
A common SNP in the uracil-DNA glycosylase gene UNG decreases ovarian cancer risk in BRCA2 mutation carriers.
Pancreatic Neoplasms
A Transcriptome-Wide Association Study (TWAS) Identifies Novel Candidate Susceptibility Genes for Pancreatic Cancer.
Papillomavirus Infections
Association of SMUG1 SNPs in Intron Region and Linkage Disequilibrium with Occurrence of Cervical Carcinoma and HPV Infection in Chinese Population.
Paraproteinemias
Uracil-DNA glycosylase activity in chronic lymphoproliferative disorders.
Photosensitivity Disorders
Roles of uracil-DNA glycosylase and apyrimidinic endonucleases in the molecular 5-bromo-2'-deoxyuridine photosensitization in Escherichia coli K-12.
Photosensitivity Disorders
Uracil-DNA glycosylase causes 5-bromodeoxyuridine photosensitization in Escherichia coli K-12.
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Uracil-DNA glycosylase activity in human acute leukemia.
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Uracil-DNA glycosylase activity in human acute leukemia.
Prostatic Neoplasms
A robust photoluminescence screening assay identifies uracil-DNA glycosylase inhibitors against prostate cancer.
Pulmonary Disease, Chronic Obstructive
Cigarette Smoke Induces Nucleic Acid Oxidation in Lung Fibroblasts.
Stomach Neoplasms
Berzosertib (VE-822) inhibits gastric cancer cell proliferation via base excision repair system.
Stomach Neoplasms
Induction of miR-31 causes increased sensitivity to 5-FU and decreased migration and cell invasion in gastric adenocarcinoma.
Stomach Neoplasms
Oxidative Stress Upregulates PDCD4 Expression in Patients with Gastric Cancer via miR-21.
Tuberculosis
Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases.
Tuberculosis
Structural plasticity in Mycobacterium tuberculosis uracil-DNA glycosylase (MtUng) and its functional implications.
Tuberculosis
Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.
Tuberculosis
Unique features of the structure and interactions of mycobacterial uracil-DNA glycosylase: structure of a complex of the Mycobacterium tuberculosis enzyme in comparison with those from other sources.
uracil-dna glycosylase deficiency
Human uracil-DNA glycosylase deficiency associated with profoundly impaired immunoglobulin class-switch recombination.
uracil-dna glycosylase deficiency
Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1.
uracil-dna glycosylase deficiency
Uracil excision by endogenous SMUG1 glycosylase promotes efficient Ig class switching and impacts on A:T substitutions during somatic mutation.
uracil-dna glycosylase deficiency
Uracil-DNA glycosylase causes 5-bromodeoxyuridine photosensitization in Escherichia coli K-12.
Vaccinia
A Conserved Tripeptide Sequence at the C Terminus of the Poxvirus DNA Processivity Factor D4 Is Essential for Protein Integrity and Function.
Vaccinia
Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA Glycosylase.
Vaccinia
Characterisation of the substrate specificity of homogeneous vaccinia virus uracil-DNA glycosylase.
Vaccinia
Crystal structure of the vaccinia virus DNA polymerase holoenzyme subunit d4 in complex with the a20 N-terminal domain.
Vaccinia
Crystal Structure of the Vaccinia Virus Uracil-DNA Glycosylase in Complex with DNA.
Vaccinia
Crystal structure of vaccinia virus uracil-DNA glycosylase reveals dimeric assembly.
Vaccinia
Mutations in active-site residues of the uracil-DNA glycosylase encoded by vaccinia virus are incompatible with virus viability.
Vaccinia
Structural analysis of point mutations at the Vaccinia virus A20/D4 interface.
Virus Diseases
Herpes simplex virus type 1 uracil-DNA glycosylase: isolation and selective inhibition by novel uracil derivatives.
Vitiligo
Association of Leukotrichia in Vitiligo and Asp148Glu Polymorphism of Apurinic/Apyrimidinic Endonuclease 1.
Waldenstrom Macroglobulinemia
Uracil-DNA glycosylase activity in chronic lymphoproliferative disorders.
Xeroderma Pigmentosum
Association Between Polymorphisms of DNA Repair Genes and Risk of Schizophrenia.
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0.0046
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.0027
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.0038
5-hydroxymethyluracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.00157 - 0.00277
uracil-containing calf thymus DNA
-
0.0007 - 0.004
uracil-mismatched double-stranded DNA with U-A mismatch
-
0.0000022 - 0.0013
uracil-mismatched double-stranded DNA with U-G mismatch
-
0.00157 - 0.00265
uracil-mismatched single-stranded DNA
-
additional information
additional information
-
0.00157
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 37°C
-
0.0021
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 37°C
-
0.0022
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 15°C
-
0.00226
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 15°C
-
0.00238
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 15°C
-
0.0024
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 22°C
-
0.00265
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 37°C
-
0.00276
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 22°C
-
0.00277
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 22°C
-
0.0007
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.004
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.0000022
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 37°C, wild-type enzyme
-
0.0004
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.0013
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.00157
uracil-mismatched single-stranded DNA
mutant enzyme G183G, at pH 7.4 and 37°C
-
0.0017
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.0021
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.4 and 37°C
-
0.0022
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hUNG2
-
0.0022
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 15°C
-
0.00265
uracil-mismatched single-stranded DNA
mutant enzyme G183G/K302R, at pH 7.4 and 37°C
-
additional information
additional information
kinetics of mutant enzymes, overview
-
additional information
additional information
-
kinetics of mutant enzymes, overview
-
additional information
additional information
SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs
-
additional information
additional information
-
SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs
-
additional information
additional information
-
UNG2 kinetics
-
additional information
additional information
UNG2 kinetics
-
additional information
additional information
-
kinetic parameters for the opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1
-
additional information
additional information
-
thermodynamics of UNG interactions with undamaged and damaged, e.g. 2'-deoxypseudouridine, substrates
-
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0.015
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.04
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
0.098
5-hydroxymethyluracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
0.0002 - 0.0003
fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G
-
pH 7.5
-
1.45 - 16.85
uracil-containing calf thymus DNA
-
0.052 - 2.28
uracil-mismatched double-stranded DNA with U-A mismatch
-
0.014 - 5.57
uracil-mismatched double-stranded DNA with U-G mismatch
-
0.29 - 46.1
uracil-mismatched single-stranded DNA
-
1.45
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 15°C
-
1.65
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 15°C
-
2.37
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 15°C
-
4.07
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 22°C
-
4.55
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 22°C
-
5.33
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 22°C
-
7.73
uracil-containing calf thymus DNA
mutant enzyme D183G, at pH 7.5 and 37°C
-
10.78
uracil-containing calf thymus DNA
wild type enzyme, at pH 7.5 and 37°C
-
16.85
uracil-containing calf thymus DNA
mutant enzyme D183G/K302R, at pH 7.5 and 37°C
-
0.052
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
2.28
uracil-mismatched double-stranded DNA with U-A mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.014
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 37°C, wild-type enzyme
-
0.055
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hSMUG1
-
5.57
uracil-mismatched double-stranded DNA with U-G mismatch
pH 7.5, 30°C, recombinant hUNG2
-
0.29
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hSMUG1
-
2.4
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 15°C
-
7.7
uracil-mismatched single-stranded DNA
mutant enzyme G183G, at pH 7.5 and 37°C
-
10.8
uracil-mismatched single-stranded DNA
wild type enzyme, at pH 7.5 and 37°C
-
16.9
uracil-mismatched single-stranded DNA
mutant enzyme G183G/K302R, at pH 7.5 and 37°C
-
46.1
uracil-mismatched single-stranded DNA
pH 7.5, 30°C, recombinant hUNG2
-
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Masaoka,A.; Matsubara, M.; Hasegawa, R.; Tanaka,T.; Kurisu,S.; Terato,H.; Ohyama,Y.; Karino, N.; Matsuda,A
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Homo sapiens (Q53HV7), Homo sapiens, Rattus norvegicus (Q811Q1)
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Homo sapiens (P13051), Homo sapiens
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brenda
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Homo sapiens
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Homo sapiens
-
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Homo sapiens (P13051)
brenda