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Information on EC 3.2.1.45 - glucosylceramidase and Organism(s) Bos taurus

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EC Tree
IUBMB Comments
Also acts on glucosylsphingosine (cf. EC 3.2.1.62 glycosylceramidase).
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This record set is specific for:
Bos taurus
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
glucocerebrosidase, imiglucerase, acid beta-glucosidase, beta-glucocerebrosidase, glucosylceramidase, alglucerase, velaglucerase alfa, taliglucerase alfa, cerezyme, glccerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acid beta-glucosidase
-
-
-
-
Alglucerase
-
-
-
-
beta-D-glucocerebrosidase
-
-
-
-
beta-glucocerebrosidase
-
-
-
-
beta-glucosylceramidase
-
-
-
-
ceramidase, glucosyl-
-
-
-
-
ceramide glucosidase
-
-
-
-
cerebroside beta-glucosidase
-
-
-
-
D-glucosyl-N-acylsphingosine glucohydrolase
-
-
-
-
GBA
-
-
glcCer-beta-glucosidase
-
-
-
-
glucocerebrosidase
glucocerebroside beta-glucosidase
-
-
-
-
glucose cerebrosidase
-
-
-
-
glucosphingosine glucosylhydrolase
-
-
-
-
glucosylceramide beta-glucosidase
-
-
-
-
glucosylcerebrosidase
-
-
-
-
glucosylsphingosine beta-D-glucosidase
-
-
-
-
glucosylsphingosine beta-glucosidase
-
-
-
-
Imiglucerase
-
-
-
-
psychosine hydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
D-glucosyl-N-acylsphingosine glucohydrolase
Also acts on glucosylsphingosine (cf. EC 3.2.1.62 glycosylceramidase).
CAS REGISTRY NUMBER
COMMENTARY hide
37228-64-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
show the reaction diagram
-
-
-
-
?
4-undecylumbelliferyl beta-D-glucopyranoside + H2O
4-undecylumbelliferone + D-glucose
show the reaction diagram
-
-
-
-
?
glucocerebroside + H2O
glucose + ceramide
show the reaction diagram
-
-
-
-
?
heptylumbelliferyl-beta-D-glucopyranoside + H2O
heptylumbelliferone + glucose
show the reaction diagram
-
-
-
-
?
nonylumbelliferyl-beta-D-glucopyranoside + H2O
nonylumbelliferone + glucose
show the reaction diagram
-
-
-
-
?
undecylumbelliferyl-beta-D-glucopyranoside + H2O
undecylumbelliferone + glucose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glucocerebroside + H2O
glucose + ceramide
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3S,4S,5R,6S)-7-oxabicyclo[4.1.0]heptane-2,3,4,5-tetrol
-
-
1-deoxynojirimycin
-
-
6-bromo-6-deoxy-conduritol
-
-
6-bromo-conduritol-beta-epoxide
-
-
gluconolactone
N-butyl-1-deoxynojirimycin
-
-
N-decyl-1-deoxynojirimycin
-
-
N-dodecyl-1-deoxynojirimycin
-
-
N-hexyl-1-deoxynojirimycin
-
-
N-methyl-1-deoxynojirimycin
-
-
N-propyl-1-deoxynojirimycin
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Activator protein
-
-
-
Dicetyl phosphate
-
-
sodium taurocholate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3 - 3.6
4-methylumbelliferyl-beta-D-glucopyranoside
0.026 - 0.045
4-nonylumbelliferyl-beta-D-glucopyranoside
0.038
4-undecylumbelliferyl beta-D-glucopyranoside
-
in presence of taurocholate
0.055
beta-D-glucocerebroside
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8 - 5.8
6.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
Gaucher disease is caused by the dysfunction of glucocerebrosidase
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLCM_BOVIN
536
0
59855
Swiss-Prot
other Location (Reliability: 5)
F1N1D5_BOVIN
536
0
59841
TrEMBL
other Location (Reliability: 5)
F1MND2_BOVIN
926
0
103812
TrEMBL
other Location (Reliability: 1)
A0A3Q1NLU8_BOVIN
848
0
95566
TrEMBL
other Location (Reliability: 1)
A0A3Q1M4V3_BOVIN
855
0
96438
TrEMBL
other Location (Reliability: 1)
A0A3Q1MU50_BOVIN
853
0
96699
TrEMBL
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
138000
-
gel filtration
56000
-
gel filtration, SDS-PAGE
59000
-
-
63000
-
SDS-PAGE
64000
-
gel filtration, SDS-PAGE
67000
-
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
-
60 min, pH 5.8, 70% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing destroys activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in COS-7 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schliemann, W.; Schliemann, B.
beta-D-Glucosidasen (EC 3.2.1.21) und glucosylceramidasen (EC 3.2.1.45) des Menschen
Pharmazie
4
243-250
1982
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Daniels, L.B.; Glew, R.H.
beta-D-Glucosidases in tissue
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
4
217-226
1984
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
-
Manually annotated by BRENDA team
Legler, G.; Liedtke, H.
Glucosylceramidase from calf spleen. Characterization of its active site with 4-n-alkylumbelliferyl beta-glucosides and N-alkyl derivatives of 1-deoxynojirimycin
Biol. Chem. Hoppe-Seyler
366
1113-1122
1985
Bos taurus
Manually annotated by BRENDA team
Reddy, P.U.M.; Murray, G.J.; Barranger, J.A.
Purification and characterization of bovine brain glucocerebrosidase
Biochem. Med.
33
200-210
1985
Bos taurus
Manually annotated by BRENDA team
Legler, G.
beta-Glucocerebrosidase: mechanistic studies with covalent and non-covalent inhibitors
NATO ASI Ser. A, Life Sci.
150
63-72
1988
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
-
Manually annotated by BRENDA team
Liedtke, H.; Legler, G.
Spleenic glucocerebrosidase and its cytosolic activator protein: effects on substrate hydrolysis and covalent inhibition by conduritol B epoxides
NATO ASI Ser. A, Life Sci.
150
353-358
1988
Bos taurus
-
Manually annotated by BRENDA team
Blech-Hermoni, Y.; Ziegler, S.; Hruska, K.; Stubblefield, B.; LaMarca, M.; Portnoy, M.; Green, E.; Sidransky, E.
In silico and functional studies of the regulation of the glucocerebrosidase gene
Mol. Genet. Metab.
99
275-282
2010
Bos taurus, Canis lupus familiaris, Homo sapiens
Manually annotated by BRENDA team