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Information on EC 3.2.1.23 - beta-galactosidase and Organism(s) Bos taurus

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EC Tree
IUBMB Comments
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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This record set is specific for:
Bos taurus
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The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-galactosidase, beta-gal, beta-d-galactosidase, senescence-associated beta-galactosidase, endo-beta-galactosidase, bglap, sa-beta-gal, acid beta-galactosidase, beta galactosidase, betagal, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid beta-galactosidase
-
-
-
-
beta-D-galactoside galactohydrolase
-
-
-
-
beta-D-glactanase
-
-
-
-
beta-D-lactosidase
-
-
-
-
beta-galactosidase
-
-
beta-galase
-
-
-
-
beta-lactosidase
-
-
-
-
driselase
-
-
-
-
Exo-(1-->4)-beta-D-galactanase
-
-
-
-
exo-beta-(1->3)-D-galactanase
-
-
-
-
galactosidase, beta
-
-
-
-
Hydrolact
-
-
-
-
lactase
-
-
-
-
lactosylceramidase II
-
-
-
-
Lactozym
-
-
-
-
Maxilact
-
-
-
-
Oryzatym
-
-
-
-
p-nitrophenyl beta-galactosidase
-
-
-
-
S 2107
-
-
-
-
SR12 protein
-
-
-
-
Sumiklat
-
-
-
-
Trilactase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-11-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
3'-deoxy-5-fluorouridine + beta-D-galactose
3'-deoxy-5'-O-beta-D-galactosyl-5-fluorouridine
show the reaction diagram
-
5-O-beta-D-galactosylfloxuridine is synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. Enzyme displays absolute regioselectivity toward the 5'-position of floxuridine. Reaction condition: pH 6.5, 45°C for 60 h
-
-
?
ceramide lacto-N-tetraose + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
glycolipid GM1 ganglioside + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
keratan sulfate + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
lactosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-galactal
-
-
D-galactose
-
-
methyl beta-galactopyranoside
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
2-nitrophenyl-beta-D-galactopyranoside
-
25°C
additional information
additional information
-
kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 0.08
2-nitrophenyl-beta-D-galactopyranoside
-
25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
-
-
6.5
-
-
7 - 7.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGAL_BOVIN
653
0
73413
Swiss-Prot
Secretory Pathway (Reliability: 1)
A0A3Q1NDQ7_BOVIN
653
0
73536
TrEMBL
Secretory Pathway (Reliability: 3)
F1MY73_BOVIN
635
1
71718
TrEMBL
Secretory Pathway (Reliability: 3)
A0A3Q1MKL4_BOVIN
726
0
82767
TrEMBL
Mitochondrion (Reliability: 5)
F1MDX6_BOVIN
660
0
75448
TrEMBL
Mitochondrion (Reliability: 5)
A5D7U5_BOVIN
653
0
73475
TrEMBL
Secretory Pathway (Reliability: 1)
F1MUF4_BOVIN
653
0
73475
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
1 * 41000
68000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30% glycerol, stable
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acid neuraminidase and beta-galactosidase are present as an enzyme complex
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Verheijen, F.; Brossmer, R.; Galjaard, H.
Purification of acid beta-galactosidase and acid neuraminidase from bovine testis: evidence for an enzyme complex
Biochem. Biophys. Res. Commun.
108
868-875
1982
Bos taurus
Manually annotated by BRENDA team
Wallenfels, K.; Weil, R.
beta-Galactosidase
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
617-663
1972
Enterobacter cloacae, Aeromonas caviae, Priestia megaterium, Bacillus subtilis, Bos taurus, Streptococcus pneumoniae, Escherichia coli, Helix pomatia, Ovis aries, Homo sapiens, Kluyveromyces lactis, Lactococcus lactis, Paracolobactrum aerogenoides, Rattus norvegicus, Saccharomyces fragilis, Shigella dysenteriae, Hamamotoa singularis, Tritrichomonas suis, Escherichia coli ML 309, Escherichia coli ML 35
-
Manually annotated by BRENDA team
Wallenfels, K.; Malhotra, O.M.
beta-Galactosidase
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
4
409-430
1960
Klebsiella aerogenes, Aspergillus flavus, Aspergillus niger, Aspergillus oryzae, Bos taurus, Canis lupus familiaris, Capra hircus, Coffea sp., Oryctolagus cuniculus, Escherichia coli, Felis catus, Helix pomatia, Ovis aries, Homo sapiens, Medicago sativa, Malus sp., Neurospora sp., Prunus dulcis, Prunus armeniaca, Prunus persica, Saccharomyces fragilis, Shigella sonnei, Escherichia coli K 12, Escherichia coli ML 308, Escherichia coli ML 309
-
Manually annotated by BRENDA team
Distler, J.J.; Jourdian, G.W.
beta-Galactosidase from bovine testes
Methods Enzymol.
50
514-520
1978
Bos taurus
Manually annotated by BRENDA team
Pilipenko, O.S.; Atyaksheva, L.F.; Poltorak, O.M.; Chukhrai, E.S.
Dissociation and catalytic activity of oligomer forms of beta-galactosidases
Russ. J. Phys. Chem. A
81
990-994
2007
Bos taurus, Kluyveromyces marxianus, Escherichia coli, Penicillium canescens, Penicillium canescens F-178
-
Manually annotated by BRENDA team
Zeng, Q.M.; Li, N.; Zong, M.H.
Highly regioselective galactosylation of floxuridine catalyzed by beta-galactosidase from bovine liver
Biotechnol. Lett.
32
1251-1254
2010
Bos taurus
Manually annotated by BRENDA team