Information on EC 3.2.1.23 - beta-galactosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.23
-
RECOMMENDED NAME
GeneOntology No.
beta-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
hyrolysis of O-glycosyl bond
-
-
O-glycosyl bond hydrolysis
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
-
-
Glycosaminoglycan degradation
-
-
Glycosphingolipid biosynthesis - ganglio series
-
-
lactose degradation II
-
-
lactose degradation III
-
-
Metabolic pathways
-
-
metabolism of disaccharids
-
-
Other glycan degradation
-
-
Sphingolipid metabolism
-
-
xyloglucan degradation II (exoglucanase)
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-11-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain HM-1 and 11-1O
-
-
Manually annotated by BRENDA team
strain F2, cold-active beta-galactosidase, gene bglA
SwissProt
Manually annotated by BRENDA team
strain 20B
-
-
Manually annotated by BRENDA team
strain C2-2
-
-
Manually annotated by BRENDA team
strain SB, gene bgaS encoding a cold-active enzyme
-
-
Manually annotated by BRENDA team
strain ATCC6276
-
-
Manually annotated by BRENDA team
strain ATCC6276
-
-
Manually annotated by BRENDA team
Aspergillus cellulosae
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain AF
-
-
Manually annotated by BRENDA team
strain AF
-
-
Manually annotated by BRENDA team
L. cv. B10
-
-
Manually annotated by BRENDA team
strain L4
-
-
Manually annotated by BRENDA team
strain RCS3
-
-
Manually annotated by BRENDA team
strain WRU 370
-
-
Manually annotated by BRENDA team
Bacillus sp. No. C-125
No. C-125
-
-
Manually annotated by BRENDA team
strain TA-11
-
-
Manually annotated by BRENDA team
obligatory anaerobic, thermophilic, Gram-gegative strain NA10
-
-
Manually annotated by BRENDA team
Bacteroides polypragmatus
-
-
-
Manually annotated by BRENDA team
ssp. lactis, strain Bb12
-
-
Manually annotated by BRENDA team
strain NCIMB41171
-
-
Manually annotated by BRENDA team
strain CCRC 15708
-
-
Manually annotated by BRENDA team
strain LL04
-
-
Manually annotated by BRENDA team
strain HL96
-
-
Manually annotated by BRENDA team
Bispora sp.
strain MEY-1
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Bullera singularis
Bullera singularis KCTC 7534
KCTC 7534
-
-
Manually annotated by BRENDA team
Caldariella acidophila
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Capsicum annum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Coffea sp.
coffee
-
-
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
strain OKN-4
-
-
Manually annotated by BRENDA team
Cucumis melo var. cantalopensis
-
-
-
Manually annotated by BRENDA team
cultivar Palava
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain B5
-
-
Manually annotated by BRENDA team
strain K 12
-
-
Manually annotated by BRENDA team
strain LMG194
Uniprot
Manually annotated by BRENDA team
strain ML 308
-
-
Manually annotated by BRENDA team
strain ML 309
-
-
Manually annotated by BRENDA team
strain ML 35
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 4214
-
-
Manually annotated by BRENDA team
strain 4214
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ATCC8005
-
-
Manually annotated by BRENDA team
strain HRI
-
-
Manually annotated by BRENDA team
strain KVE39
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain R1
-
-
Manually annotated by BRENDA team
strain R1
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Hymenaea courbari
-
-
-
Manually annotated by BRENDA team
a legume tree from the Neotropical region of the world
-
-
Manually annotated by BRENDA team
i.e. Polyporus tulipiferae
-
-
Manually annotated by BRENDA team
Kerstingiella geocarpa
-
-
-
Manually annotated by BRENDA team
Kestingiella geocarpa
-
-
-
Manually annotated by BRENDA team
strain MW 190-9B
-
-
Manually annotated by BRENDA team
strain B57 and A60
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain DSM5418
-
-
Manually annotated by BRENDA team
Kluyveromyces sp.
-
-
-
Manually annotated by BRENDA team
three strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
strain 2607
-
-
Manually annotated by BRENDA team
ssp. lactis, two strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
two strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
two strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
several strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
CNRZ 131
-
-
Manually annotated by BRENDA team
CNRZ 131
-
-
Manually annotated by BRENDA team
three strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
two strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
strain L461
-
-
Manually annotated by BRENDA team
strain MEP181R88, isolated form human faeces
-
-
Manually annotated by BRENDA team
two strains, isolated form human faeces
-
-
Manually annotated by BRENDA team
strain MEP181R91, isolated form human faeces
-
-
Manually annotated by BRENDA team
strain MEP181R91, isolated form human faeces
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Leuconostoc citrovorum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 74A
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus gasseri
-
-
-
Manually annotated by BRENDA team
no activity in Phaeobacter gallaeciensis BS107
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii JC2049
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus XSM19
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Paracolobactrum aerogenoides
-
-
-
Manually annotated by BRENDA team
strain F-178
-
-
Manually annotated by BRENDA team
strain F-178
-
-
Manually annotated by BRENDA team
NCAIM 00237
-
-
Manually annotated by BRENDA team
Penicillium glaucum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Penicillium toxidarium
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. PDR14
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Planococcus sp.
-
SwissProt
Manually annotated by BRENDA team
almond
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
TAE 79
SwissProt
Manually annotated by BRENDA team
TAE 79
SwissProt
Manually annotated by BRENDA team
strain 22b
Uniprot
Manually annotated by BRENDA team
strain 22b
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 4S
-
-
Manually annotated by BRENDA team
strain 4S
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Rhizomucor sp.
-
-
-
Manually annotated by BRENDA team
Rhizopus acidus
-
-
-
Manually annotated by BRENDA team
Rhizopus formosciensis
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Saccharomyces anamensis
-
-
-
Manually annotated by BRENDA team
strain Y187
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
Saccharomyces fragilis KY5463
strain KY5463
-
-
Manually annotated by BRENDA team
Saccharomyces lactis
-
-
-
Manually annotated by BRENDA team
strain V2-2
-
-
Manually annotated by BRENDA team
Scopulariopsis sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Sporobolomyces singularis
-
-
-
Manually annotated by BRENDA team
Sporotrichum sp.
-
-
-
Manually annotated by BRENDA team
strain CBS8119
-
-
Manually annotated by BRENDA team
strain NCTC 12261T
-
-
Manually annotated by BRENDA team
Streptococcus mitis NCTC 12261T
strain NCTC 12261T
-
-
Manually annotated by BRENDA team
strain 6646 K
-
-
Manually annotated by BRENDA team
strain 6646 K
-
-
Manually annotated by BRENDA team
serotype 2, isolate JX081101
C5VV31
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain MT-4
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
IAM 12617
-
-
Manually annotated by BRENDA team
IAM 12617
-
-
Manually annotated by BRENDA team
UST050418-052
-
-
Manually annotated by BRENDA team
UST050418-052
-
-
Manually annotated by BRENDA team
strain TP6-B1
-
-
Manually annotated by BRENDA team
strain TP6-B1
-
-
Manually annotated by BRENDA team
Thermoanaerobium sp.
strain 2905
-
-
Manually annotated by BRENDA team
Thermoanaerobium sp. 2905
strain 2905
-
-
Manually annotated by BRENDA team
CBS 236.58
-
-
Manually annotated by BRENDA team
CBS 236.58
-
-
Manually annotated by BRENDA team
strain YT-I
-
-
Manually annotated by BRENDA team
strain 4-1A
-
-
Manually annotated by BRENDA team
strain A4
-
-
Manually annotated by BRENDA team
Torula thermophila
-
-
-
Manually annotated by BRENDA team
Torulopsis sphaerica
strain J28
-
-
Manually annotated by BRENDA team
Torulopsis sphaerica J28
strain J28
-
-
Manually annotated by BRENDA team
Torulopsis versatilis
strain M6
-
-
Manually annotated by BRENDA team
Torulopsis versatilis M6
strain M6
-
-
Manually annotated by BRENDA team
Reiter strain
-
-
Manually annotated by BRENDA team
Reiter strain
-
-
Manually annotated by BRENDA team
strain FTKO-39, two isozymes BGT I and BGT II
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
L. cv. Egret
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
a metagenomic library from topsoil of Daqing oil field of Heilongjiang Province in China is constructed for the screening of clones with beta-galactosidase activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Zygosaccharomyces lactis
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-glycerol-O-D-beta-galactopyranoside + H2O
beta-D-galactose + glycerol
show the reaction diagram
-
-
-
-
?
1-naphthyl beta-D-galactopyranoside + H2O
1-naphthol + beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
show the reaction diagram
1-naphthyl-beta-D-glucoside + H2O
beta-D-glucose + 1-naphthol
show the reaction diagram
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside + H2O
2,4-dinitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl-beta-D-galactoside + H2O
2,4-dinitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl-beta-D-glucoside + H2O
2,4-dinitrophenol + beta-D-glucose
show the reaction diagram
-
activity is 10000 times lower than with 2,4-dinitrophenyl-beta-D-galactoside
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
the enzyme also shows beta-fucosidase activity
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
show the reaction diagram
2-nitrophenyl-alpha-D-mannopyranoside + H2O
2-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl-beta-D-fucopyranoside + H2O
2-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
?
2-phenylethyl-beta-D-galactoside + H2O
2-phenylethanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
3'-deoxy-5-fluorouridine + beta-D-galactose
3'-deoxy-5'-O-beta-D-galactosyl-5-fluorouridine
show the reaction diagram
-
5-O-beta-D-galactosylfloxuridine is synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. Enzyme displays absolute regioselectivity toward the 5'-position of floxuridine. Reaction condition: pH 6.5, 45C for 60 h
-
-
-
3,4-dinitrophenyl beta-D-galactopyranoside + H2O
3,4-dinitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
3-nitrophenyl beta-D-galactopyranoside + H2O
3-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
4-methylumbelliferyl-alpha-L-D-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
4-methylumbelliferone + beta-D-galactopyranose
show the reaction diagram
-
pH 4.0, 30 min, 37C
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
100% activity
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
show the reaction diagram
42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-arabinoside + H2O
4-nitrophenol + beta-D-arabinose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
show the reaction diagram
3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
show the reaction diagram
43% relative to 2-nitrophenyl-beta-D-galactoside hydrolysis
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
low activity
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + beta-D-mannose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
show the reaction diagram
-
1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-L-arabinoside + H2O
4-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
34.49% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
4-nitrophenyl-beta-D-galactoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
4-nitrophenyl-beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucurone
show the reaction diagram
-
0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-beta-L-arabinoside + H2O
4-nitrophenol + beta-L-arabinose
show the reaction diagram
-
0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
show the reaction diagram
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indoxyl-beta-galactoside + H2O
5-bromo-4-chloro-3-indoxol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-dodecanoylaminofluorescein di-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
7-O-beta-D-galactosylresorufin + H2O
resorufin + beta-D-galactose
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-D-galactopyranoside + H2O
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-ol) + beta-D-galactose
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactoside + H2O
?
show the reaction diagram
-
assays are performed on individual Escherichia coli beta-galactosidase molecules at 2 different concentrations of the substrate DDAO-beta-D-galactoside using a free zone capillary electrophoresis-based protocol with an in-laboratory-constructed instrument utilizing laser-induced fluorescence detection
-
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
show the reaction diagram
agarose + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
allolactose + H2O
D-galactose + D-glucose
show the reaction diagram
asialo alpha1-acid glycoprotein + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
asialofetuin + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
beta-(1->3)-D-galactan + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
beta-1,3-galactobiose + H2O
beta-D-galactose
show the reaction diagram
beta-1,3-galactotriitol + H2O
?
show the reaction diagram
-
-
-
-
?
beta-1,3-galactotriose + H2O
?
show the reaction diagram
beta-1,6-galactobiose + H2O
beta-D-galactose
show the reaction diagram
-
-
-
-
?
beta-1,6-galactotriitol + H2O
?
show the reaction diagram
-
-
-
-
?
beta-1,6-galactotriose + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-(1-6) galactobiose + H2O
?
show the reaction diagram
beta-D-galactosyl azide + H2O
azide + beta-D-galactose
show the reaction diagram
-
-
-
-
?
beta-D-galactosyl fluoride + H2O
fluoride + beta-D-galactose
show the reaction diagram
-
-
-
-
?
butyl O-beta-galactoside + H2O
butanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-galactose
show the reaction diagram
ceramide lacto-N-tetraose + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
chlorophenol red beta-D-galactopyranoside + H2O
chlorophenol red + beta-D-galactose
show the reaction diagram
-
-
-
-
?
digalactosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
ethyl-beta-D-galactoside + H2O
ethanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
exopolysaccharide + H2O
beta-D-galactose + ?
show the reaction diagram
-
modified exopolysaccharides produced by Lactococcus lactis subsp. cremonis B39 and B891
-
-
?
fluorescein-beta-D-digalactopyranoside + H2O
fluorescein + beta-D-galactose
show the reaction diagram
-
-
-
-
?
Gal-alpha(1,3)-Gal-beta-2,4-dinitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
galactan + H2O
?
show the reaction diagram
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
galactan + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
galactan + H2O
galactose
show the reaction diagram
-
-
+ small amounts of glucose and arabinose
-
galactobiose + H2O
?
show the reaction diagram
-
-
-
?
galactosyl-fetuin + H2O
galactose + fetuin
show the reaction diagram
-
-
-
-
?
galactosyl-ovomucoid + H2O
galactose + ovomucoid
show the reaction diagram
-
-
-
-
?
galactotetraose + H2O
beta-D-galactose
show the reaction diagram
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
galactoxyloglucan + H2O
galactose + ?
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
14.3% of the activity with lactose
-
-
?
glycoasparagines + H2O
galactose + ?
show the reaction diagram
-
hydrolysis of beta-galactosyl linkages in urinary glycoasparagines
-
?
glycolipid GM1 ganglioside + H2O
beta-D-galactose + ?
show the reaction diagram
keratan sulfate + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
lactitol + H2O
D-galactose + D-glucitol
show the reaction diagram
lactose + 2-phenylethanol
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + alpha-D-methyl-galactoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + alpha-D-methyl-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + arabinose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Hyp-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Ser-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Thr-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + cellobiose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + fructose
?
show the reaction diagram
lactose + glucose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
show the reaction diagram
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
lactose + H2O
D-galactose + D-glucose + oligosaccharide
show the reaction diagram
Bispora sp.
under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423)
the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
lactose + H2O
galactooligosaccharides
show the reaction diagram
lactose + H2O+
D-glucose + D-galactose
show the reaction diagram
lactose + hydroquinone
hydroquinone galactoside + D-glucose
show the reaction diagram
lactose + lactulose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + lyxose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + myo-inositol
6-beta-O-D-galactopyranosyl myo-inositol + ?
show the reaction diagram
-
-
-
?
lactose + p-nitrophenyl beta-D-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + rhamnose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + sucrose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + xylose
?
show the reaction diagram
lactosucrose + H2O
D-galactose + ?
show the reaction diagram
lactosyl ceramide + H2O
?
show the reaction diagram
lactulose + H2O
?
show the reaction diagram
lactulose + H2O
D-fructose + D-galactose
show the reaction diagram
lactulose + H2O
D-galactose + D-fructose
show the reaction diagram
methyl beta-D-galactohexaoside + H2O
?
show the reaction diagram
methyl beta-D-galactopentaoside + H2O
?
show the reaction diagram
methyl beta-D-galactotetraoside + H2O
?
show the reaction diagram
methyl O-beta-galactoside + H2O
methanol + beta-D-galactose
show the reaction diagram
methyl salicylate beta-D-galactoside + H2O
methyl salicylate + beta-D-galactose
show the reaction diagram
-
-
-
-
?
methyl-beta-D-galactopyranoside + H2O
methanol + beta-D-galactose
show the reaction diagram
methylthio-beta-D-galactoside + H2O
galactose + methanethiol
show the reaction diagram
monogalactosyldiacylglycerol + H2O
galactose + diacylglycerol
show the reaction diagram
-
-
-
?
monosialoganglioside + H2O
?
show the reaction diagram
-
-
-
-
?
O-beta-D-galactosyl-1,3-D-arabinoside + H2O
beta-D-galactose + D-arabinose
show the reaction diagram
o-nitrophenyl beta-D-fucopyranoside + H2O
o-nitrophenol + beta-D-fucose
show the reaction diagram
Planococcus sp.
6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside
-
-
?
o-nitrophenyl beta-D-fucoside + H2O
o-nitrophenol + beta-D-fucose
show the reaction diagram
o-nitrophenyl beta-D-galactopyranoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
o-nitrophenyl-beta-D-galactoside + myo-inositol
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl alpha-arabinofuranoside + H2O
p-nitrophenol + alpha-arabinose
show the reaction diagram
-
weak activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-glucopyranoside + H2O
p-nitrophenol + alpha-D-glucose
show the reaction diagram
p-nitrophenyl alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
show the reaction diagram
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 49.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
show the reaction diagram
-
beta-galactosidase I: 20.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 32.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 24.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 12.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 18.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
show the reaction diagram
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
-
beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-galactopyranoside + H2O
p-nitrophenol + alpha-L-galactose
show the reaction diagram
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 3.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucose
show the reaction diagram
p-nitrophenyl beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
p-nitrophenyl beta-D-glucuronide + H2O
p-nitrophenol + ?
show the reaction diagram
-
beta-galactosidase I: 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 15.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 64% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
show the reaction diagram
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylose
show the reaction diagram
p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
p-nitrophenol + N-acetyl-beta-D-glucosamine
show the reaction diagram
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 10.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-galactoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + D-glucose
lactose + p-nitrophenol
show the reaction diagram
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + glucose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl-beta-D-galactoside + myo-inositol
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + p-nitrophenyl beta-D-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + sucrose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + xylose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
60% of the activity with p-nitrophenyl-beta-D-galactoside
-
-
?
pectic galactan + H2O
?
show the reaction diagram
-
-
-
-
?
pectic galactan + H2O
galactose + ?
show the reaction diagram
-
-
-
-
?
pectic polysaccharides + H2O
?
show the reaction diagram
-
1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
phenyl-alpha-D-galactoside + H2O
phenol + alpha-D-galactose
show the reaction diagram
-
14% of the activity with o-nitrophenyl-beta-D-galactoside
-
-
?
phenyl-beta-D-galactopyranoside + H2O
phenol + beta-D-galactose
show the reaction diagram
phenyl-beta-D-galactoside + H2O
phenol + beta-D-galactose
show the reaction diagram
phlorizin + H2O
D-galactose + ?
show the reaction diagram
-
-
-
-
?
resorufin-beta-D-galactoside + H2O
?
show the reaction diagram
-
-
-
-
?
tetrahexosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
thio-(o-nitrophenyl)-beta-D-galactoside + H2O
thio-(o-nitrophenol) + beta-D-galactose
show the reaction diagram
-
-
-
-
?
trihexosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
-
structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
xyloglucan oligosaccharides + H2O
?
show the reaction diagram
lactose
additional information
-