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Information on EC 3.2.1.21 - beta-glucosidase and Organism(s) Arabidopsis thaliana

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EC Tree
IUBMB Comments
Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
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This record set is specific for:
Arabidopsis thaliana
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-glucosidase, linamarase, beta-glu, emulsin, bglu1, zm-p60.1, bgl1b, cel3a, novozyme 188, t-cell inhibitor, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amygdalase
-
-
-
-
amygdalinase
-
-
-
-
arbutinase
-
-
-
-
aryl-beta-glucosidase
-
-
-
-
beta-1,6-glucosidase
-
-
-
-
beta-D-glucosidase
-
-
-
-
beta-D-glucoside glucohydrolase
beta-glucosidase
beta-glucoside hydrolase
-
-
-
-
BGA
-
-
-
-
cellobiase
-
-
-
-
elaterase
-
-
-
-
emulsin
-
-
-
-
gentiobiase
-
-
-
-
limarase
-
-
-
-
Novozyme 188
-
-
-
-
p-nitrophenyl beta-glucosidase
-
-
-
-
primeverosidase
-
-
-
-
salicilinase
-
-
-
-
T-cell inhibitor
-
-
-
-
vicianase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-glucoside glucohydrolase
Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-22-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
show the reaction diagram
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
4-methylumbelliferol + beta-D-glucopyranose
show the reaction diagram
163% relative activity
-
-
?
4-MU-glucoside + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
amygdalin + H2O
prunasin + D-glucose
show the reaction diagram
48% relative activity
-
-
?
beta-gentiobiose + H2O
?
show the reaction diagram
6.3% relative activity
-
-
?
D(+)cellobiose + H2O
?
show the reaction diagram
6.3% relative activity
-
-
?
DIMBOA-glucoside + H2O
?
show the reaction diagram
-
-
-
?
esculin + H2O
?
show the reaction diagram
-
-
-
?
n-decyl-beta-D-glucopyranoside + H2O
n-decanol + beta-D-glucopyranose
show the reaction diagram
11% relative activity
-
-
?
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + beta-D-glucopyranose
show the reaction diagram
17% relative activity
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + beta-D-glucopyranose
show the reaction diagram
7% relative activity
-
-
?
o-nitrophenyl-beta-D-fucopyranoside + H2O
o-nitrophenol + beta-D-fucopyranose
show the reaction diagram
117% relative activity
-
-
?
o-nitrophenyl-beta-D-galactopyranoside + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
17% relative activity
-
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucopyranose
show the reaction diagram
71% relative activity
-
-
?
prunasin + H2O
D-mandelonitrile + beta-D-glucose
show the reaction diagram
39% relative activity
-
-
?
salicin + H2O
?
show the reaction diagram
4.4% relative activity
-
-
?
scopolin + H2O
scopoletin + beta-D-glucose
show the reaction diagram
-
-
-
?
scopolin + H2O
scopoletin + D-glucose
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
scopolin + H2O
scopoletin + beta-D-glucose
show the reaction diagram
-
-
-
?
scopolin + H2O
scopoletin + D-glucose
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
gluconolactone
competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates
jacalin-related lectin
-
isoform Pyk10 is localized in ER bodies and has beta-D-glucosidase and beta-D-fucosidase activities. Jacalin-related lectins regulate the size of the active Pyk10 complexes, with two types of lectins working antagonistically
-
p-nitrophenyl-1-thio-beta-D-glucopyranoside
competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
jacalin-related lectin
-
isoform Pyk10 is localized in ER bodies and has beta-D-glucosidase and beta-D-fucosidase activities. Jacalin-related lectins regulate the size of the active Pyk10 complexes, with two types of lectins working antagonistically
-
protein PBPI
binding protein, interacts with beta-glucosidases and activates them
-
protein PBPII
binding protein, interacts with beta-glucosidases and activates them
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.78
4-methylumbelliferyl-beta-D-glucopyranoside
-
5.8 - 9.7
esculin
2.1
o-nitrophenyl-beta-D-glucopyranoside
-
1.9
p-nitrophenyl-beta-D-glucopyranoside
-
0.73 - 0.81
scopolin
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 4.7
gluconolactone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.9
-
crude extract, at pH 5.5 and 50°C
148.5
-
after 164.5fold purification, at pH 5.5 and 50°C
20.34
21fold purified recombinant beta-glucosidase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
recombinant mutated beta-glucosidase
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
mutated recombinant beta-glucosidase
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isoform Pyk10 is localized in ER bodies and has beta-D-glucosidase and beta-D-fucosidase activities. Jacalin-related lectins regulate the size of the active Pyk10 complexes, with two types of lectins working antagonistically
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGL04_ARATH
512
0
57971
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL05_ARATH
500
0
56645
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL06_ARATH
379
0
42587
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL07_ARATH
502
0
56418
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL08_ARATH
497
1
56178
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL09_ARATH
506
1
56892
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL10_ARATH
508
0
57078
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL11_ARATH
521
0
58062
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL12_ARATH
507
1
56966
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL13_ARATH
507
1
56957
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL14_ARATH
489
0
55009
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL15_ARATH
506
0
56905
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL16_ARATH
514
0
58043
Swiss-Prot
Mitochondrion (Reliability: 3)
BGL17_ARATH
517
1
59121
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL19_ARATH
527
0
60015
Swiss-Prot
Secretory Pathway (Reliability: 3)
BGL20_ARATH
535
1
61675
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL21_ARATH
524
0
59664
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL22_ARATH
524
0
59781
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL23_ARATH
524
0
59721
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL24_ARATH
533
0
61034
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL26_ARATH
560
1
63916
Swiss-Prot
Mitochondrion (Reliability: 4)
BGL27_ARATH
540
0
62450
Swiss-Prot
other Location (Reliability: 5)
BGL28_ARATH
582
0
67299
Swiss-Prot
Secretory Pathway (Reliability: 3)
BGL29_ARATH
590
0
68244
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL40_ARATH
510
0
58125
Swiss-Prot
Secretory Pathway (Reliability: 3)
BGL41_ARATH
535
1
61463
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL42_ARATH
490
0
56077
Swiss-Prot
Mitochondrion (Reliability: 4)
BGL43_ARATH
501
0
57244
Swiss-Prot
other Location (Reliability: 5)
BGL44_ARATH
512
0
58983
Swiss-Prot
Secretory Pathway (Reliability: 5)
BGL45_ARATH
520
0
59897
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL46_ARATH
516
0
59113
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL47_ARATH
535
1
61962
Swiss-Prot
Secretory Pathway (Reliability: 5)
BGL30_ARATH
577
0
66920
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL31_ARATH
534
0
61872
Swiss-Prot
Secretory Pathway (Reliability: 3)
BGL32_ARATH
534
0
61684
Swiss-Prot
Secretory Pathway (Reliability: 4)
BGL33_ARATH
614
0
70190
Swiss-Prot
Secretory Pathway (Reliability: 2)
BGL34_ARATH
511
0
57542
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL35_ARATH
511
0
57468
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL38_ARATH
541
0
61133
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL01_ARATH
517
0
58585
Swiss-Prot
Secretory Pathway (Reliability: 1)
BGL02_ARATH
299
0
33646
Swiss-Prot
other Location (Reliability: 4)
BGL03_ARATH
507
0
57307
Swiss-Prot
Secretory Pathway (Reliability: 1)
A0A178U5A7_ARATH
760
0
82019
TrEMBL
other Location (Reliability: 2)
F4JAB3_ARATH
608
0
66098
TrEMBL
other Location (Reliability: 2)
A0A5S9XIT9_ARATH
611
0
66888
TrEMBL
other Location (Reliability: 2)
A0A654FHG4_ARATH
611
0
66891
TrEMBL
other Location (Reliability: 2)
Q501D0_ARATH
581
0
63003
TrEMBL
other Location (Reliability: 3)
A0A7G2EV96_ARATH
1194
0
130295
TrEMBL
other Location (Reliability: 3)
A0A178UQ98_ARATH
490
0
56260
TrEMBL
Mitochondrion (Reliability: 2)
A0A7G2EQR9_ARATH
585
0
64053
TrEMBL
other Location (Reliability: 2)
A0A654G6E3_ARATH
490
0
56077
TrEMBL
Mitochondrion (Reliability: 4)
A0A654FEF8_ARATH
579
0
63158
TrEMBL
other Location (Reliability: 3)
A0A178U900_ARATH
3043
0
332894
TrEMBL
Mitochondrion (Reliability: 3)
A0A1I9LR68_ARATH
449
0
49222
TrEMBL
other Location (Reliability: 3)
A0A178V693_ARATH
609
0
66310
TrEMBL
other Location (Reliability: 2)
A0A1I9LMA2_ARATH
430
0
46828
TrEMBL
other Location (Reliability: 2)
A0A178VDC7_ARATH
608
0
66336
TrEMBL
other Location (Reliability: 3)
Q9SD72_ARATH
609
0
66265
TrEMBL
other Location (Reliability: 2)
A0A5S9Y9M6_ARATH
507
0
58305
TrEMBL
Mitochondrion (Reliability: 4)
A0A1I9LQK9_ARATH
611
0
66860
TrEMBL
other Location (Reliability: 2)
A0A1I9LMA1_ARATH
450
0
49102
TrEMBL
other Location (Reliability: 2)
A0A178VP22_ARATH
611
0
66841
TrEMBL
other Location (Reliability: 2)
A0A5S9XJA7_ARATH
608
0
66318
TrEMBL
other Location (Reliability: 3)
A0A5S9XIM5_ARATH
609
0
66326
TrEMBL
other Location (Reliability: 2)
A0A1I9LQL0_ARATH
449
0
49309
TrEMBL
other Location (Reliability: 2)
Q9SD73_ARATH
608
0
66309
TrEMBL
other Location (Reliability: 3)
Q9SD69_ARATH
636
0
69649
TrEMBL
other Location (Reliability: 2)
A0A178URS6_ARATH
487
0
55947
TrEMBL
Mitochondrion (Reliability: 2)
A0A1I9LR69_ARATH
431
0
47129
TrEMBL
other Location (Reliability: 2)
F4JAB7_ARATH
644
0
70583
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
SDS-PAGE
59700
59800
BGLU 22, calculated from seuqence
60000
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase
63000
SDS-PAGE, recombinant glycosylated protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
mutated recombinant beta-glucosidase is fairly stable at pH 5.0 and 8.5, exhibiting 60 and 62% activities, respectively. It retains over 50% of the original activity between pH 4-7.5. Between pH values 6.0 and 7.2, it retains over 98% activity after incubation at 37°C for 2 h
696166
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
is fairly stable in 50 mM sodium acetate buffer, pH 5.6, at temperatures up to 42°C, while retaining only 48% of the original activity at 50°C for 10 min. It is completely inactivated upon incubation at 60°C for 10 min
50
dramatical decrease of activity above 50°C
60 - 90
-
about 50% activity at 60°C, about 30% activity at 70°C, about 10% activity at 80°C, no activity at 90°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Concanavalin A-Sepharose column chromatography, Shodex-phenyl HIC-814 column chromatography, and Sephacryl S-200 gel filtration
-
recombinant beta-glucosidase purified by gel filtration to apparent homogeneity, 21fold with an overall enzyme yield of 6.8%
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA expressed in insect cells, Baculovirus expression system
cDNA is expressed in insect cells, Baculovirus expression system
native and mutated cDNA sequences cloned into vector pPICZalphaB and expressed in Pichia pastoris
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
BGLU21 by cold, 2,4-dichlorophenoxyacetate and methyl jasmonate
BGLU22 by cold and mannitol
BGLU22 by NaCl, 2,4-dichlorophenoxyacetate and methyl jasmonate
BGLU23 by 2,4-dichlorophenoxyacetate and methyl jasmonate
BGLU23 by NaCl and mannitol
senescence-induced loss in photosynthesis enhances cell wall beta-glucosidase activity
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Turan, Y.
A pseudo-beta-glucosidase in Arabidopsis thaliana: correction by site-directed mutagenesis, heterologous expression, purification, and characterization
Biochemistry (Moscow)
73
912-919
2008
Arabidopsis thaliana (Q9SLA0), Arabidopsis thaliana
Manually annotated by BRENDA team
Nagano, A.J.; Fukao, Y.; Fujiwara, M.; Nishimura, M.; Hara-Nishimura, I.
Antagonistic jacalin-related lectins regulate the size of ER body-type beta-glucosidase complexes in Arabidopsis thaliana
Plant Cell Physiol.
49
969-980
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Sherameti, I.; Venus, Y.; Drzewiecki, C.; Tripathi, S.; Dan, V.M.; Nitz, I.; Varma, A.; Grundler, F.M.; Oelmueller, R.
PYK10, a beta-glucosidase located in the endoplasmatic reticulum, is crucial for the beneficial interaction between Arabidopsis thaliana and the endophytic fungus Piriformospora indica
Plant J.
54
428-439
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Mohapatra, P.K.; Patro, L.; Raval, M.K.; Ramaswamy, N.K.; Biswal, U.C.; Biswal, B.
Senescence-induced loss in photosynthesis enhances cell wall beta-glucosidase activity
Physiol. Plant.
138
346-355
2010
Arabidopsis thaliana
Manually annotated by BRENDA team
Ahn, Y.O.; Shimizu, B.; Sakata, K.; Gantulga, D.; Zhou, C.; Zhou, Z.; Bevan, D.R.; Esen, A.
Scopolin-hydrolyzing beta-glucosidases in roots of Arabidopsis
Plant Cell Physiol.
51
132-43
2010
Arabidopsis thaliana (Q9C525), Arabidopsis thaliana (Q9C8Y9), Arabidopsis thaliana (Q9SR37)
Manually annotated by BRENDA team