Information on EC 3.2.1.18 - exo-alpha-sialidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
3.2.1.18
-
RECOMMENDED NAME
GeneOntology No.
exo-alpha-sialidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
-
-
-
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
active site, Arg498, Glu258, Tyr317, and Tyr262 are catalytic residues, with the aromatic ring of the latter being important for function
Q9Q2W5
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
enzyme does not act on 4-O-acetylated sialic acids
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
exo-glycosidase, active center contains essential cysteine residues
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
exo-glycosidase, the active center contains essential cysteine residues
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
reaction and kinetic mechanisms
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
trans-sialidase preferring the transfer of sialic acids from sialoglycoconjugates to beta-galactosyl acceptor molecules, ping-pong, double diplacement catalytic mechanism, Tyr342 is important, Asp59 is the acid/base residue, enzyme-substrate complex structure, covalent sialyl-enzyme intermediate
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
catalytic chemistry involves the formation of a covalent intermediate with an active site tyrosine nucleophile
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
catalytic mechanism, overview
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
ping-pong bi-bi kinetic mechanism. Residue D59 functions as acid/base catalyst, and Y342 as active site nucleophile
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
show the reaction diagram
exo-glycosidase
Clostridium sordellii G12
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Other glycan degradation
-
-
Sphingolipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
acetylneuraminyl hydrolase
The enzyme does not act on 4-O-acetylated sialic acids. endo-alpha-Sialidase activity is listed as EC 3.2.1.129, endo-alpha-sialidase. See also EC 4.2.2.15 anhydrosialidase.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetylneuraminidase
-
-
-
-
Acetylneuraminyl hydrolase
-
-
-
-
acylneuraminyl glycohydrolase
-
-
-
-
alpha-neuraminidase
-
-
-
-
Cytosolic sialidase
-
-
-
-
G9 sialidase
-
-
-
-
Ganglioside sialidase
-
-
-
-
Lysosomal sialidase
-
-
-
-
Major 85 kDa surface antigen
-
-
-
-
Major surface antigen
-
-
-
-
Membrane sialidase
-
-
-
-
Mouse skeletal muscle sialidase
-
-
-
-
MSS
-
-
-
-
MTS
-
-
-
-
mucopolysaccharide N-acetylneuraminylhydrolase
-
-
-
-
Murine thymic sialidase
-
-
-
-
N-acylneuraminate glycohydrolase
-
-
-
-
NANase
-
-
-
-
neuraminidase
-
-
-
-
SA85-1.1 protein
-
-
-
-
SA85-1.2 protein
-
-
-
-
SA85-1.3 protein
-
-
-
-
sialidase
-
-
-
-
STNA
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9001-67-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aeromonas sp. 40/02
-
-
-
Manually annotated by BRENDA team
sialidase isoenzyme NA1; sialidase isoenzyme NA2
-
-
Manually annotated by BRENDA team
Arthrobacter sialophilus
-
-
-
Manually annotated by BRENDA team
non-pathogenic strains
-
-
Manually annotated by BRENDA team
strains L, M1, M2, and M1057
-
-
Manually annotated by BRENDA team
Aspergillus fumigatus ATCC 13073
-
UniProt
Manually annotated by BRENDA team
starfish
-
-
Manually annotated by BRENDA team
Bacteroides fragilis SBT3182
SBT3182
-
-
Manually annotated by BRENDA team
Bacteroides thetaiotaomicron VPI5482
-
UniProt
Manually annotated by BRENDA team
brain
Uniprot
Manually annotated by BRENDA team
spleen
-
-
Manually annotated by BRENDA team
testis
-
-
Manually annotated by BRENDA team
Jakari strain
-
-
Manually annotated by BRENDA team
Clostridium chauvoei Jakari
Jakari strain
-
-
Manually annotated by BRENDA team
Clostridium chauvoei NC08596
NC08596
-
-
Manually annotated by BRENDA team
commercial product
Uniprot
Manually annotated by BRENDA team
Clostridium perfringens CN3718
type D
-
-
Manually annotated by BRENDA team
Clostridium perfringens type A
type A
-
-
Manually annotated by BRENDA team
strain G12
-
-
Manually annotated by BRENDA team
Clostridium sordellii G12
strain G12
-
-
Manually annotated by BRENDA team
strain KCTC3075
UniProt
Manually annotated by BRENDA team
Corynebacterium diphtheriae KCTC3075
strain KCTC3075
UniProt
Manually annotated by BRENDA team
i.e. Erysipelothrix insidosa
-
-
Manually annotated by BRENDA team
H7N9 influenza virus
-
UniProt
Manually annotated by BRENDA team
H7N9 influenza virus A/Anhui/1/2013
-
UniProt
Manually annotated by BRENDA team
H7N9 influenza virus A/Shanghai/1/2013
-
UniProt
Manually annotated by BRENDA team
3 isozymes Neu-1, Neu-2, and Neu-3
-
-
Manually annotated by BRENDA team
acute ill patients with and without sepsis
-
-
Manually annotated by BRENDA team
gene neu-1
-
-
Manually annotated by BRENDA team
isoform Neu1
SwissProt
Manually annotated by BRENDA team
isoform Neu3
-
-
Manually annotated by BRENDA team
isozyme NEU2
-
-
Manually annotated by BRENDA team
isozyme NEU3
-
-
Manually annotated by BRENDA team
human parainfluenza virus
types 1 to 3
-
-
Manually annotated by BRENDA team
alpha-sialidase, influenza A/Tokyo/3/67
-
-
Manually annotated by BRENDA team
avain virus type H5N1
-
-
Manually annotated by BRENDA team
avian influenzy virus
-
-
Manually annotated by BRENDA team
diverse strains, overview
-
-
Manually annotated by BRENDA team
H3N2, strain X-31, reassorting of influenza A/Victoria/3/75 and influenza A/PR/8/34, from inoculated chicken egg allantoic fluid
-
-
Manually annotated by BRENDA team
isolated from fertilised hen eggs, Vero and Madin darby canine kidney cells
-
-
Manually annotated by BRENDA team
strain A/Jinan/15/90, type H3N2; strain A/PR/8/34, type H1N1
-
-
Manually annotated by BRENDA team
strain A/N1/PR/8/34
-
-
Manually annotated by BRENDA team
strain A/South Carolina/1/1918 H1N1
UniProt
Manually annotated by BRENDA team
strain A/tokyo/3/67, type H2N2
-
-
Manually annotated by BRENDA team
subtype N1
-
-
Manually annotated by BRENDA team
type H5N1
-
-
Manually annotated by BRENDA team
type N2, several strains
-
-
Manually annotated by BRENDA team
H1N1, strain A/Brisbane/59/2007-like
-
-
Manually annotated by BRENDA team
influenza A virus A/California/05/2009/H1N1
-
-
-
Manually annotated by BRENDA team
influenza A virus A/Hong Kong/156/97/H5N1
-
-
-
Manually annotated by BRENDA team
influenza A virus A/Jinan/15/90
strain A/Jinan/15/90, type H3N2
-
-
Manually annotated by BRENDA team
influenza A virus A/N1/PR/8/34
strain A/N1/PR/8/34
-
-
Manually annotated by BRENDA team
influenza A virus A/PR/8/34
strain A/PR/8/34, type H1N1
-
-
Manually annotated by BRENDA team
influenza A virus A/South Carolina/1/1918 H1N1
strain A/South Carolina/1/1918 H1N1
UniProt
Manually annotated by BRENDA team
influenza A virus A/tokyo/3/67
strain A/tokyo/3/67, type H2N2
-
-
Manually annotated by BRENDA team
influenza A virus H1N1
-
-
-
Manually annotated by BRENDA team
influenza A virus H1N1
-
UniProt
Manually annotated by BRENDA team
influenza A virus H5N1
-
-
-
Manually annotated by BRENDA team
influenza A virus H5N1
-
UniProt
Manually annotated by BRENDA team
influenza A virus H5N1
avian influenzy virus
-
-
Manually annotated by BRENDA team
influenza A virus H5N1
type H5N1
-
-
Manually annotated by BRENDA team
influenza A virus H7N7
-
-
-
Manually annotated by BRENDA team
influenza A virus N1
N1
-
-
Manually annotated by BRENDA team
influenza A virus newCaledonia/20/99(H1N1)
-
UniProt
Manually annotated by BRENDA team
from Vero cells, strain B/Hong Kong/548/2000
SwissProt
Manually annotated by BRENDA team
from Vero cells, strain B/Switzerland/4291/97
SwissProt
Manually annotated by BRENDA team
isolated from fertilised hen eggs, Vero and Madin darby canine kidney cells
-
-
Manually annotated by BRENDA team
strain B/Jiangsu/10/2003
-
-
Manually annotated by BRENDA team
strain B/Memphis/3/89
-
-
Manually annotated by BRENDA team
strains B/Memphis/20/96, B/Memphis/20-152K/96, B/Hong Kong/45/2005, and B/Hong Kong/36/2005
Uniprot
Manually annotated by BRENDA team
influenza B virus B/Jiangsu/10/2003
strain B/Jiangsu/10/2003
-
-
Manually annotated by BRENDA team
influenza B virus B/Kochi/41/2011
-
-
-
Manually annotated by BRENDA team
influenza B virus B/Kochi/59/2011
-
-
-
Manually annotated by BRENDA team
influenza B virus B/Kochi/60/2011
-
-
-
Manually annotated by BRENDA team
influenza B virus B/Memphis/3/89
strain B/Memphis/3/89
-
-
Manually annotated by BRENDA team
Macrobdella sp.
-
-
-
Manually annotated by BRENDA team
strain Urabe AM9
-
-
Manually annotated by BRENDA team
Mumps virus Urabe AM9
strain Urabe AM9
-
-
Manually annotated by BRENDA team
3 isozymes Neu-1, Neu-2, and Neu-3
-
-
Manually annotated by BRENDA team
isoform Neu1
UniProt
Manually annotated by BRENDA team
Clone 30 strain
Uniprot
Manually annotated by BRENDA team
strain GX-04/05
UniProt
Manually annotated by BRENDA team
strain Herts/33; strain ZJ-01/00
UniProt
Manually annotated by BRENDA team
strain JS-01/05
UniProt
Manually annotated by BRENDA team
strain JS-02/05
UniProt
Manually annotated by BRENDA team
strain JS-03/05
UniProt
Manually annotated by BRENDA team
strain JS-06/05
UniProt
Manually annotated by BRENDA team
strain JS-07/05
UniProt
Manually annotated by BRENDA team
strain JS-08/05
UniProt
Manually annotated by BRENDA team
strain JS-09/05
UniProt
Manually annotated by BRENDA team
strain JS-10/05
UniProt
Manually annotated by BRENDA team
strain La Sota
UniProt
Manually annotated by BRENDA team
strain Mukteswar
UniProt
Manually annotated by BRENDA team
strain ZhJ-3/97
-
-
Manually annotated by BRENDA team
strains GX-01/05, GX-02/05, and GX-03/05
UniProt
Manually annotated by BRENDA team
strains JS-04/05 and JS-05/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus Anhinga/US(FL)/44083/93
NDV
-
-
Manually annotated by BRENDA team
Newcastle disease virus Clone 30
Clone 30 strain
Uniprot
Manually annotated by BRENDA team
Newcastle disease virus GX-04/05
strain GX-04/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus Herts/33
strain Herts/33
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-01/05
strain JS-01/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-02/05
strain JS-02/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-03/05
strain JS-03/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-06/05
strain JS-06/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-07/05
strain JS-07/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-08/05
strain JS-08/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-09/05
strain JS-09/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus JS-10/05
strain JS-10/05
UniProt
Manually annotated by BRENDA team
Newcastle disease virus La Sota
strain La Sota
UniProt
Manually annotated by BRENDA team
Newcastle disease virus Mukteswar
strain Mukteswar
UniProt
Manually annotated by BRENDA team
Newcastle disease virus V4
strain V4
UniProt
Manually annotated by BRENDA team
Newcastle disease virus ZhJ-3/97
strain ZhJ-3/97
-
-
Manually annotated by BRENDA team
Newcastle disease virus ZJ-01/00
strain ZJ-01/00
UniProt
Manually annotated by BRENDA team
no activity in Escherichia phage K1-ind(1)
-
-
-
Manually annotated by BRENDA team
isoform Neu3a; Nile tilapia
UniProt
Manually annotated by BRENDA team
isoform Neu3d; Nile tilapia
UniProt
Manually annotated by BRENDA team
isoform Neu3e; Nile tilapia
UniProt
Manually annotated by BRENDA team
medaka
UniProt
Manually annotated by BRENDA team
gene nanB, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview
SwissProt
Manually annotated by BRENDA team
gene nanH, from fowl cholera isolates, quantitiative relation of the 2 sialidase ativities, encoded by genes nanB and nanH, in different isolates, overview
SwissProt
Manually annotated by BRENDA team
Pseudomonas aeruginosa Mu 15
Mu 15
-
-
Manually annotated by BRENDA team
K1-dep phage virion: K1-dep phages require the K1 capsule for infection
-
-
Manually annotated by BRENDA team
Streptococcus pneumoniae IID553
-
-
-
Manually annotated by BRENDA team
groups A and B
-
-
Manually annotated by BRENDA team
typ III group B
-
-
Manually annotated by BRENDA team
UF 245 group A
-
-
Manually annotated by BRENDA team
Streptococcus sp. K6646
K6646
-
-
Manually annotated by BRENDA team
strain 2009 H1N1
-
-
Manually annotated by BRENDA team
swine influenza virus 2009 H1N1
strain 2009 H1N1
-
-
Manually annotated by BRENDA team
-
Q37QH2
UniProt
Manually annotated by BRENDA team
11 isozymes of trans-sialidase 1, gene TS1
UniProt
Manually annotated by BRENDA team
strain Y
-
-
Manually annotated by BRENDA team
trans-sialidase
-
-
Manually annotated by BRENDA team
Trypanosoma cruzi Y
strain Y
-
-
Manually annotated by BRENDA team
isolated from blood of infected camels, STIB 731-AA, IL 1392
-
-
Manually annotated by BRENDA team
Agai strain; Agari strain
-
-
Manually annotated by BRENDA team
Trypanosoma vivax Agai
Agai strain
-
-
Manually annotated by BRENDA team
Trypanosoma vivax Agari
Agari strain
-
-
Manually annotated by BRENDA team
1918 H1N1 influenzy virus, 18NA
-
-
Manually annotated by BRENDA team
A/Viet Nam/DT-036/2005 type H5N1
-
-
Manually annotated by BRENDA team
H5N1 avian influenza virus
-
-
Manually annotated by BRENDA team
unidentified influenza virus A (H3N2)
A (H3N2)
-
-
Manually annotated by BRENDA team
unidentified influenza virus H1N1/09
-
-
-
Manually annotated by BRENDA team
unidentified influenza virus H5
H5
-
-
Manually annotated by BRENDA team
unidentified influenza virus H5N1
H5N1
-
-
Manually annotated by BRENDA team
unidentified influenza virus H5N1
type H5N1
-
-
Manually annotated by BRENDA team
unidentified influenza virus H7
H7
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
O35657
the absence of Neu1 results in the increased sialylation of the cell surface proteins, probably affecting multiple receptors for phagocytosis. Macrophages from the Neu1-deficient mice show increased sialylation and impaired phosphorylation of FcgammaRas well as markedly reduced phosphorylation of Syk kinase in response to treatment with immunoglobulin G-opsonized beads
malfunction
-
mutations of residues expected to interact directly with the sialic acid N5-acetyl (A160, M87, I105) and C7-C9 glycerol side-chain (E113, Y179, Y181) reduce enzymatic activity. Truncations at the N- or C-terminus of more than 10 residues abolish enzyme activity
malfunction
-
neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, from A/California/05/2009/H1N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview, neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5, from A/Hong Kong/156/97/H5N1) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview
malfunction
-
mutations in the NEU1 gene are causative of sialidosis, a severe lysosomal storage disorder showing autosomal recessive mode of inheritance
malfunction
influenza A virus A/California/05/2009/H1N1
-
neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, from A/California/05/2009/H1N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview, neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5, from A/Hong Kong/156/97/H5N1) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview
-
malfunction
influenza A virus A/Hong Kong/156/97/H5N1
-
neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, from A/California/05/2009/H1N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview, neuraminidase insertion mutant virus shows increased particles formation, in pseudotyped particles system, activity compared to the wild-type virus. Compared with the wild-type AH N1, the wild-type 09N1 exhibits higher neuraminidase activity and releases more pseudoparticles. Deletion/insertion of the 09s60 segment does not alter this relationship. The infectivity of pseudoparticles harboring neuraminidase in combination with the hemagglutinin from HPAI H5N1 (AH H5, from A/Hong Kong/156/97/H5N1) is decreased by insertion of 09s60 into AH N1 and is increased by deletion of 09s60 from 09N1, the mutation also alters the oligomeric structure, overview
-
metabolism
-
NEU3 regulates the detergent resistant membranes ganglioside content and is as a modulator of Akt phosphorylation
physiological function
-
neuraminidase enhances the initial steps of human T-cell leukemia virus type 1 replication
physiological function
-
the NanA neuraminidase of Streptococcus pneumoniae is involved in biofilm formation
physiological function
-
the NanI and NanJ sialidases of Clostridium perfringens are not essential for virulence
physiological function
-
trans-sialidase plays a key role in the recognition and invasion of host cells and in enabling the parasite to escape the human immune response
physiological function
O35657
treatment of the cells with purified mouse Neu1 reduces surface sialylation and restores phagocytosis. Cell surface Neu1 activates the phagocytosis in macrophages and dendritic cells through desialylation of surface receptors, thus, contributing to their functional integrity
physiological function
-
attachment of Newcastle disease virus to the host cell is mediated by the haemagglutinin-neuraminidase, a multifunctional protein that has receptor recognition, neuraminidase and fusion promotion activities. Multiple regions of the haemagglutinin-neuraminidase protein globular head are important for fusion promotion, and wild-type levels of neuraminidase activity are not absolutely required for viral infection
physiological function
-
the middle region of the HPIV2 F protein contains the site(s) that determines its specificity for the HPIV2 hemagglutinin-neuraminidase protein
physiological function
-
exogenous neuraminidase from Arthrobacter ureafaciens mediates the diffusion and penetration of swine influenza virus in respiratory mucus
physiological function
-
isoform NEU1 restrains the endothelial migratory response to wounding
physiological function
Q37QH2
the enzyme contributes to the virulence of Treponema denticola. The enzyme protects Treponema denticola from serum killing by preventing the deposition of membrane attack complexes on the bacterial cell surface
physiological function
-
the enzyme plays a pivotal role in bacterial proliferation
physiological function
-
the proline-rich region of NEU4 long can enhance cell proliferation and retinoic acid-induced neuronal differentiation and it is also involved in NEU4 interaction with Akt, as well as in substrate recognition, modifying directly or through the interaction with other protein(s) the enzyme specificity toward sialylated glycoprotein(s)
physiological function
Trypanosoma cruzi Y
-
trans-sialidase plays a key role in the recognition and invasion of host cells and in enabling the parasite to escape the human immune response
-
physiological function
Newcastle disease virus Anhinga/US(FL)/44083/93
-
attachment of Newcastle disease virus to the host cell is mediated by the haemagglutinin-neuraminidase, a multifunctional protein that has receptor recognition, neuraminidase and fusion promotion activities. Multiple regions of the haemagglutinin-neuraminidase protein globular head are important for fusion promotion, and wild-type levels of neuraminidase activity are not absolutely required for viral infection
-
physiological function
Clostridium perfringens type A
-
the NanI and NanJ sialidases of Clostridium perfringens are not essential for virulence
-
physiological function
-
the enzyme contributes to the virulence of Treponema denticola. The enzyme protects Treponema denticola from serum killing by preventing the deposition of membrane attack complexes on the bacterial cell surface
-
metabolism
-
NEU4 long is a downstream component of the PI3K/Akt signaling pathway required for retinoic acid-induced differentiation of neuroblastoma SK-N-BE cells
additional information
Q7TG96
low-glycosylation in och1-defective Pichia pastoris enhances the immunogenicity of recombinant neuraminidase and elicits similar antibody titers with less antigen when compared with hyper- and non-glycosylated neuraminidases after vaccination of mice
additional information
-
the amino terminal region of the PIV5 hemagglutinin-neuraminidase protein determines the F protein specificity in promoting cell-cell fusion
additional information
Q7YZT3
trans-sialidase 1 model structure complexed with the Sia derivative 3-fluoro-5-N-acetyl-9-benzamido-2,9-dideoxy-neuraminic acid
additional information
influenza A virus newCaledonia/20/99(H1N1)
-
low-glycosylation in och1-defective Pichia pastoris enhances the immunogenicity of recombinant neuraminidase and elicits similar antibody titers with less antigen when compared with hyper- and non-glycosylated neuraminidases after vaccination of mice
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
N-glycolylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
N-glycolylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
(trifluoromethyl)umbelliferyl alpha-sialoside + H2O
(trifluoromethyl)umbelliferone + sialic acid
show the reaction diagram
-
-
-
-
?
(trifluoromethyl)umbelliferyl alpha-sialoside + lactose
(trifluoromethyl)umbelliferone + sialyllactose
show the reaction diagram
-
-
-
-
?
2'-(4-methyl-umbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Corynebacterium diphtheriae, Corynebacterium diphtheriae KCTC3075
C6JSW6
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
Newcastle disease virus, Newcastle disease virus Anhinga/US(FL)/44083/93
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Q07599
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
O35657
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Trypanosoma cruzi Y
-
-
-
-
?
2-(3-methoxyphenyl)-N-acetylneuraminic acid + H2O
3-methoxyphenol + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(3-methoxyphenyl)-N-acetylneuraminic acid + H2O
3-methoxyphenol + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
Q59310
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
synthetic, fluorogenic substrate
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
Clostridium sordellii G12
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetyl-alpha-D-neuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
unidentified influenza virus H5N1
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
show the reaction diagram
-
-
-
-
?
2-deoxy-2,3-dehydro-N-acetylneuraminic acid + H2O
N-acetylneuraminic acid
show the reaction diagram
Q59310
-
reaction deduced from crystal structures and confirmed by NMR study
-
?
2-O-(3-methoxyphenyl)neuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)Galf(beta1,4)GlcNAc
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)Galf(beta1,4)GlcNAcOBn
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)Galf(beta1,4)GlcNAcol
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Gal
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]GalOBn
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galol
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAc
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)GlcNAc
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)GlcNAcOBn
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)GlcNAcol
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)[Galf(beta1,4)]GlcNA
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)[Galf(beta1,4)]GlcNAc
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
?
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + H2O
sialic acid + ?
show the reaction diagram
L0N7M7
-
-
-
?
3'-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
3'-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
3'-sialyllactose + mucin oligosaccharide
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O
?
show the reaction diagram
Aspergillus fumigatus, Aspergillus fumigatus ATCC 13073
Q4WQS0
preferred substrate
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Q8AAK9
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
?
show the reaction diagram
Aspergillus fumigatus, Aspergillus fumigatus ATCC 13073
Q4WQS0
-
-
-
?
4-methylumbelliferyl alpha-NeuAc + H2O
4-methylumbelliferone + alpha-neuAc
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-sialoside + H2O
?
show the reaction diagram
Corynebacterium diphtheriae, Corynebacterium diphtheriae KCTC3075
C6JSW6
48.9% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside + H2O
4-methylumbelliferol + N-acetylneuraminate + 4-methylumbelliferyl alpha-D-galactopyranoside
show the reaction diagram
-
preference for the alpha2-3 isomer of 4-methylumbelliferyl alpha-D-N-acetylneuraminylgalactopyranoside
-
-
?
4-methylumbelliferyl-3-deoxy-D-glycero-D-galacto-2-nonulosonic acid + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
A9YLL2, C3W5S3
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Q37QH2
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
H7N9 influenza virus
R4JGE3, R4RZI6
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
influenza A virus H1N1
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
influenza A virus H1N1
C3W5S3
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
H7N9 influenza virus A/Shanghai/1/2013
R4RZI6
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
influenza A virus H7N7
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
influenza A virus H5N1
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
influenza A virus H5N1
A9YLL2
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
Q37QH2
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
show the reaction diagram
H7N9 influenza virus A/Anhui/1/2013
R4JGE3
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
Q9Q2W5
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
isozyme Neu-1 and Neu-3
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
sialic acid + lactose
show the reaction diagram
L0N7M7
-
-
-
?
4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + N-acetylneuraminic acid
show the reaction diagram
Q8AAK9
-
-
-
?
4-nitrophenyl alpha-sialoside + H2O
4-nitrophenol + sialic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-sialoside + H2O
?
show the reaction diagram
Corynebacterium diphtheriae, Corynebacterium diphtheriae KCTC3075
C6JSW6
65.5% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-nitrophenyl alpha-sialoside + lactose
4-nitrophenol + sialyllactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
about 30% cleavage
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
about 5% cleavage
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
less than 10% cleavage
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
less than 5% cleavage
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
about 50% cleavage
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
about 35% cleavage
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
5-acetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-acetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
5-acetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-acetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
5-azidoacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-azidoacetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
5-azidoacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-azidoacetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
?
show the reaction diagram
Clostridium perfringens, Clostridium perfringens type A
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
5-bromo-4-chloro-3-indole + N-acetylneuraminic acid
show the reaction diagram
Clostridium perfringens, Clostridium perfringens CN3718
-
-
-
-
?
5-fluoroacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
5-fluoroacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
6'-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
L0N7M7
-
-
-
?
6'-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
6'-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
isoform Neu3a shows low activity
-
-
?
alpha(2-3')-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Q9EZV6, Q9EZV7
nanH encoded enzyme is highly specific for the (2-3')-sialyllactose
-
-
?
alpha(2-3')-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Q9EZV6, Q9EZV7
the nanB encoded enzyme hydrolyzes both 2-3'- and 2-6'-sialyllactose
-
-
?
alpha(2-3)-linked sialic acid + H2O
?
show the reaction diagram
-
the enzyme is selective for alpha(2-3)-linked sialic acid
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
high activity
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
formation of a covalent sialyl-enzyme intermediate
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
preference for alpha(2-3)-linkages
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Corynebacterium diphtheriae, Corynebacterium diphtheriae KCTC3075
C6JSW6
89.8% activity compared to alpha(2-6)-sialyllactose
-
-
?
alpha(2-3)sialyl-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
alpha(2-3)sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)sialylparagloboside + H2O
?
show the reaction diagram
-
-
-
-
?
alpha(2-6')-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Q9EZV6, Q9EZV7
the nanB encoded enzyme hydrolyzes both (2-3')- and (2-6')-sialyllactose, the latter substrate is preferred
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
low activity
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
C6JSW6
100% activity
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
DELTA15Pd2,6ST-catalyzed trans-sialidase reactions follow a ping-pong bi-bi reaction mechanism, DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Corynebacterium diphtheriae KCTC3075
C6JSW6
100% activity
-
-
?
alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
?
show the reaction diagram
Aspergillus fumigatus, Aspergillus fumigatus ATCC 13073
Q4WQS0
-
-
-
?
alpha-acid glycoprotein + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
alpha-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-phenyl-N-acetylneuraminoside + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
-
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Clostridium chauvoei NC08596
-
-
-
-
-
alpha-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
Clostridium sordellii G12
-
-
-
-
?
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI5482
Q8AAK9
-
-
-
?
alpha2,3-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
apotransferrin + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
bovine brain gangliosides + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
bovine gland submaxillary gland mucin + H2O
?
show the reaction diagram
-
-
-
-
?
bovine submaxillary gland mucin + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
bovine submaxillary gland mucin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
bovine submaxillary mucin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
Candida albicans cells + H2O
desialylated Candida albicans cells + sialic acid
show the reaction diagram
-
-
-
-
?
casein glycomacropeptide + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
casein glycomacropeptide + lactose
3'-sialyllactose + ?
show the reaction diagram
-
-
-
-
?
Collocalia mucoid + H2O
sialic acid + ?
show the reaction diagram
-
substrate contains a large number of terminal sialic acids
-
-
?
colomic acid + H2O
sialic acid + lactose
show the reaction diagram
-
low activity
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI5482
Q8AAK9
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
Bacteroides fragilis SBT3182
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
unidentified influenza virus A (H3N2)
-
-
-
-
?
colominic acid + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
sialic acid + lactose
show the reaction diagram
Q9EZV6, Q9EZV7
i.e. (2-8')-sialyllactose
-
-
?
envelope glycoprotein + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
-
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Macrobdella sp.
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Q8AAK9
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus
R4JGE3, R4RZI6
-
-
-
?
fetuin + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
isoform Neu3a shows low activity
-
-
?
fetuin + H2O
?
show the reaction diagram
Bacteroides thetaiotaomicron VPI5482
Q8AAK9
-
-
-
?
fetuin + H2O
?
show the reaction diagram
influenza A virus H1N1
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus A/Shanghai/1/2013
R4RZI6
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Trypanosoma vivax Agai
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Trypanosoma vivax Agari
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Clostridium chauvoei Jakari
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus A/Anhui/1/2013
R4JGE3
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Pseudomonas aeruginosa Mu 15
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
Q80A54, Q80A58
-
-
-
?
fetuin + H2O
Neu5Ac + ?
show the reaction diagram
Trypanosoma vivax, Trypanosoma vivax Agai, Trypanosoma vivax Agari
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
show the reaction diagram
-
i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
show the reaction diagram
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
ganglioside GD1a + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
ganglioside GD1a + H2O
sialic acid + ganglioside GM1
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
-
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1b + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ganglioside GD3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD3 + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
ganglioside GD3 + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
isoform Neu3a shows highest activity with ganglioside GD3
-
-
?
ganglioside GM1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM2 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM2 + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
-
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
ganglioside GM3 + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
sialic acid + ?
show the reaction diagram
-
best substrate
-
-
?
ganglioside GM3 + H2O
sialic acid + ?
show the reaction diagram
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
ganglioside GM3(Neu5Ac) + H2O
desialylated ganglioside GM3 + sialic acid
show the reaction diagram
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside GM3(Neu5Gc) + H2O
desialylated ganglioside GM3 + sialic acid
show the reaction diagram
-
i.e. Neu5Gcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside GQ1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GT1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GT1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GT1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GT1b + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GT1b + H2O
sialic acid + ?
show the reaction diagram
Q9EZV6, Q9EZV7
-
-
-
?
ganglioside IV3-alpha-Neu5Ac-nLc4Cer + H2O
desialylated ganglioside nLc4Cer + sialic acid
show the reaction diagram
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)GcNAcbeta(1-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside VI3-alpha-Neu5Ac-nLc6Cer + H2O
desialylated ganglioside nLc6Cer + sialic acid
show the reaction diagram
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)GcNAcbeta(1-3)-Galbeta(1-4)-GlcNAcbeta(1-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
glucomacropeptide + H2O
?
show the reaction diagram
-
-
-
-
?
glucomacropeptide + H2O
?
show the reaction diagram
-
sialidase isoenzyme NA1, sialidase isoenzyme NA2
-
-
?
glucomacropeptide + H2O
?
show the reaction diagram
Aeromonas sp. 40/02
-
-
-
-
?
lactosyl-ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-5,9-diacetyl neuraminic acid
?
show the reaction diagram
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-N-acetyl heptulosonic acid
?
show the reaction diagram
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-N-acetyl neuraminic acid
?
show the reaction diagram
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-N-acetyl octulosonic acid
?
show the reaction diagram
-
-
-
-
?
mucin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
mucin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
N-(2,4,7,8,9-penta-O-acetyl-N-acetyl-beta-D-neuraminoyl)phenylalanine methylester + H2O
?
show the reaction diagram
-
-
-
-
?
N-(4,7,8,9-tetra-O-acetyl-N-acetyl-2-O-benzyl-alpha-D-neuraminoyl)phenylalanine methylester + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
N-acetylneuraminic acid-alpha-2,6-D-galactose + H2O
N-acetylneuraminic acid + D-galactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
N-glycoloylneuraminic acid-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5(4-[(2,4-dinitrophenoxy)methyl]-1H-1,2,3-triazol-1-yl)Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5(azido)Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5,9-diAc(2-3)-alpha-lactose + H2O
?
show the reaction diagram
-
low activity
-
-
?
Neu5,9Ac2(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5,9Ac2(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5,9Ac2(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
Neu5,9Ac2(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
Neu5,9Ac2(alpha2,6)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5,9Ac2(alpha2,6)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
Neu5,9Ac2(alpha2,6)Galbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
Neu5,9Ac2(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5,9Ac2(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
weak activity
-
-
?
Neu5,9Ac2(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
Neu5,9Ac2alpha2,6Galbeta-p-nitrophenol + H2O
Neu5,9Ac2alpha2,6Gal + p-nitrophenol
show the reaction diagram
-
low activity
-
-
?
Neu5Ac(2-3)-alpha-lactose + H2O
Neu5Ac + lactose
show the reaction diagram
Trypanosoma vivax, Trypanosoma vivax Agai, Trypanosoma vivax Agari
-
-
-
-
?
Neu5Ac(2-6)-alpha-lactose + H2O
Neu5Ac + lactose
show the reaction diagram
Trypanosoma vivax, Trypanosoma vivax Agai, Trypanosoma vivax Agari
-
-
-
-
?
Neu5Ac(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5Ac(alpha2,3)Gal + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5Ac(alpha2,3)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5Acalpha2,3Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,3)lactose + H2O
Neu5Ac + lactose
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,6)-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,6)Galbeta-p-nitrophenol + H2O
Neu5Ac(alpha2,6)Gal + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5Ac(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
Neu5Ac(alpha2,6)GalNAc + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-(CH2)8COOCH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-CH2(CH3)CH2CH2CH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-CH2Ph + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-butyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-N3 + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
Neu5Ac + 4-nitrophenyl beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
Neu5Ac-alpha-(2->6)-Gal-beta-4-nitrophenol + H2O
Neu5Ac + 4-nitrophenyl beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
Neu5Ac9(2,4-dinitrobenzoyl)N-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac9N-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Ac9N3-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Acalpha(2->)3Galbeta(1->)4GlcNAc + H2O
?
show the reaction diagram
unidentified influenza virus, unidentified influenza virus H1N1/09
-
-
-
-
?
Neu5Acalpha(2->3)9Galbeta(1->4)GlcNAc + H2O
?
show the reaction diagram
unidentified influenza virus, unidentified influenza virus H1N1/09
-
-
-
-
?
Neu5Acalpha(2->3)Galbeta-4-nitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Acalpha(2->3)Galbeta-4-nitrophenyl + H2O
?
show the reaction diagram
P29768
-
-
-
?
Neu5Acalpha(2->3)LacNAcbeta-4-aminophenyl + H2O
?
show the reaction diagram
influenza A virus, influenza A virus H5N1
-
best substrate
-
-
?
Neu5Acalpha(2->6)Galbeta4-nitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
Neu5Acalpha(2->6)Galbeta4-nitrophenyl + H2O
?
show the reaction diagram
P29768
-
-
-
?
Neu5Acalpha(2->6)LacNAcbETA-4-aminophenyl + H2O
?
show the reaction diagram
influenza A virus, influenza A virus H5N1
-
-
-
-
?
Neu5Acalpha2,6Galbeta-p-nitrophenol + H2O
Neu5Acalpha2,6Galbeta + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Neu5AcF-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
Neu5AcF + 4-nitrophenyl beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc + H2O
2 Neu5Gcalpha
show the reaction diagram
-
-
-
-
?
Neu5Gcalpha-(2-8)-Neu5Ac + H2O
Neu5Gc + Neu5Ac
show the reaction diagram
-
preferred substrate
-
-
?
Neu5Gcalpha-(2-8)-Neu5Gc + H2O
Neu5Gc + Neu5Gc
show the reaction diagram
-
-
-
-
?
Neu5Gcalpha-(2-8)-Neu5Gc + H2O
Neu5Gc + Neu5Gc
show the reaction diagram
-
low activity
-
-
?
Neu5glycolyl-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
Neu5glycolylneuraminate + 4-nitrophenyl beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium + H2O
sialic acid + 3,4-dimethylpyridine
show the reaction diagram
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium + H2O
sialic acid + 3-methylpyridine
show the reaction diagram
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium + H2O
sialic acid + 4-methylpyridine
show the reaction diagram
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium + H2O
sialic acid + pyridine
show the reaction diagram
-
-
-
-
?
octyl-ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
octyl-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
orosomucoid + H2O
?
show the reaction diagram
-
-
-
-
?
orosomucoid + H2O
?
show the reaction diagram
-
-
-
-
?
orosomucoid + H2O
?
show the reaction diagram
-
-
-
-
?
orosomucoid + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
ovomucin + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-5,9-diacetyl neuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-N-acetyl heptulosonic acid + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-N-acetyl neuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-N-acetyl octulosonic acid + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl alpha-sialoside + H2O
?
show the reaction diagram
Q02834
-
-
-
?
phenyl beta-sialoside + H2O
phenol + alpha-sialic acid
show the reaction diagram
Q02834
mutant enzymes Y370G, Y370A, Y370N and Y370T catalyze the hydrolysis of phenyl beta-sialoside with an inversion of the anomeric configuration. Mutant enzyme Y370A shows 4% of the activity of Y370G, mutant enzymes Y370N and Y370T show 9% of the activity of Y370G
-
-
?
phenyl beta-sialoside + lactose
phenol + sialyllactose
show the reaction diagram
-
-
-
-
-
phenyl beta-sialoside + lactose
phenol + sialyllactose
show the reaction diagram
Q02834
the Y370G mutant can transfer the sialic acid moiety from phenyl beta-sialoside to lactose in yields of up to 13%13%. Greater than 90% of the sialyllactose product formed in the coupling reactions is the alpha-2,6-isomer.
-
-
?
poly-alpha-2,8-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
polysialic acid + H2O
sialic acid
show the reaction diagram
-
-
-
-
?
polysialic acid + H2O
sialic acid
show the reaction diagram
-
preference for cleavage of polymers with (-5-O-glycolylNeu5Gc2-)-units, also cleaves polymers with (-8Neu5Acalpha2-)- and (-8Neu5Gcalpha2-)-units
-
-
?
polysialic acid + H2O
?
show the reaction diagram
-
-
-
-
?
red blood ghost cells + H2O
?
show the reaction diagram
-
desialylation, preference for different species: in descending order mouse, rat, camel, goat, and dog
-
-
?
sialic acid tetramer with alpha-linked trifluoromethylumbelliferyl + H2O
?
show the reaction diagram
-
hydrolysis with formation of two alpha-linked trifluoromethylumbelliferyl glycoside-containing species, trifluoromethylumbelliferone and the TFMU sialoside monomer
-
-
?
sialic acid trimer with alpha-linked trifluoromethylumbelliferyl + H2O
?
show the reaction diagram
-
degraded with exclusive cleavage at the coumarin-sialoside bond
-
-
?
sialyl-alpha-(2-8)-sialic acid + H2O
sialic acid
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,3-lactose + H2O
?
show the reaction diagram
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI5482
Q8AAK9
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
sialyl-alpha-2,6-lactose + H2O
?
show the reaction diagram
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI5482
Q8AAK9
-
-
-
?
sialyloligosaccharides + H2O
?
show the reaction diagram
-
-
-
-
?
submaximally mucin + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
-
-
-
?
submaximally mucin + H2O
?
show the reaction diagram
A0A024FS29, A0A024FSF5, I3JCG9
isoform Neu3a shows low activity
-
-
?
transferrin + H2O
?
show the reaction diagram
-
-
-
-
?
transferrin + H2O
?
show the reaction diagram
-
-
-
-
?
transferrin + H2O
?
show the reaction diagram
Q8AAK9
-
-
-
?
transferrin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
transferrin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
vesicular GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
monosialoganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
Q9EZV6, Q9EZV7
substrate specificity
-
-
-
additional information
?
-
-
enzyme is involved in the metabolism of gangliosides
-
-
-
additional information
?
-
-
enzyme is required for cellular signaling and for the production of cytokine interleukin-4 by activated T-lymphocytes, inherited enzyme defiency results in sialidosis or galactosialidosis, both severe metaboic disorders associated with lysosomal storage of oligosaccharides and glycopeptides
-
-
-
additional information
?
-
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification, enzyme is responsible for decomposition of organic material in various biotopes
-
-
-
additional information
?
-
-
essential enzyme, enzyme containing culture filtrate possesses the ability to aggluinate and virus and shows receptor properties for erythrocytes, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
-
essential enzyme, enzyme containing culture filtrate possesses the ability to agglutinate and virus and shows receptor properties for erythrocytes, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
Q9EZV6, Q9EZV7
most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection
-
-
-
additional information
?
-
Q9EZV6, Q9EZV7
most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection
-
-
-
additional information
?
-
-
the enzyme modifies the ganglioside pattern of adjacent cells, involved in cell-cell-interaction
-
-
-
additional information
?
-
-
colominic acid, and gangliosides GM1 and GD1 are poor substrates
-
-
-
additional information
?
-
-
enzyme agglutinates erythrocytes from human blood
-
-
-
additional information
?
-
-
ganglioside GM1 and bovine serum albumin are poor substrates, no activity with 4-methylumbelliferyl-alpha-3-deoxy-D-glycero-D-galacto-2-nonulosonic acid
-
-
-
additional information
?
-
-
ganglioside substrate specificity of the isozymes, overview
-
-
-
additional information
?
-
Q9Q2W5
multifunctional enzyme with sialidase, receptor-binding, and fusion promotion activities
-
-
-
additional information
?
-
-
no activity with sialyl-alpha-(2-8)-sialic acid
-
-
-
additional information
?
-
Q80A54, Q80A58
plaque formation on MDCK and Vero cells at different temperatures, investigation of influence of different hemagglutinin and neuraminidase combinations on enzyme activity
-
-
-
additional information
?
-
Q80A54, Q80A58
plaque formation on MDCK and Vero cells, investigation of influence of different hemagglutinin and neuraminidase combinations on enzyme activity
-
-
-
additional information
?
-
-
preference for terminally (2-3)-sialylated substrates, gangliosides GD1a, GD1b, and GT1b are poor substrates
-
-
-
additional information
?
-
-
spontaneous hydrolysis of pyridinium salts of alpha-D-N-acetylneuraminic acid occurs via 2 different ways, effects on the enzymic reaction mechanism are investigated
-
-
-
additional information
?
-
Q9EZV6, Q9EZV7
substrate specificity, no or poor activity with ganglioside GM1, colominic acid, and alpha(2-6')-sialyllactose
-
-
-
additional information
?
-
-
the dimers Neu5Gcalpha-(2-8)-Neu5Ac and Neu5Gcalpha-(2-8)-Neu5Gc are poor substrates
-
-
-
additional information
?
-
-
the enzyme also performs transglycosylation reactions
-
-
-
additional information
?
-
-
usage of a glucomacropeptide from milk
-
-
-
additional information
?
-
-
egg or Madin Darby canine kidney cell isolated virus strains tend to exhibit highest activity against 3'-sialyl-bound sialic acid whereas Vero isolated strain favour 6'-sialyl-(N-acetyllactosamine)-bound sialic acid
-
-
-
additional information
?
-
-
hardly any activities on alpha(2-6)-sialyllactose and ganglioside GM1 and GM2
-
-
-
additional information
?
-
Q54727
NanB may contribute to Strreptococcus pneumoniae colonization and pathogenesis
-
-
-
additional information
?
-
-
sialidase-3 is a key enzyme for ganglioside hydrolysis. Neu3 is an important regulator of insulin sensitivity and glucose tolerance
-
-
-
additional information
?
-
-
the Neu3 gene represents a physiological modulator of vascular smooth muscle cells responses that may contribute to plaque instability in atherosclerosis
-
-
-
additional information
?
-
-
the enzyme cleaves sialic acids from RBC ghosts of sheep, horse, goat, cattle, pig and mice as well as mouse brain cells
-
-
-
additional information
?
-
-
the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI
-
-
-
additional information
?
-
-
increased CD15 and decreased CD15s expression during myeloid differentiation is sialidase dependent. Both CD15s synthesis and sialidase activity are induced by CD44 ligation, but the increase in sialidase activity dominates, such that the overall expression pattern upon CD44 ligation is a decrease in CD15s expression with an accompanying increase in CD15. The observed increase in CD15 results predominantly from conversion of CD15s to CD15 on glycoproteins
-
-
-
additional information
?
-
-
neuraminidase causes the desialylation of both PDGF and IGF-1 receptors and diminishes the intracellular signals induced by the mitogenic ligands PDGF-BB and IGF-2
-
-
-
additional information
?
-
Q54727
Streptococcus pneumoniae is able to utilize complex N-linked human glycoconjugates as a sole source of carbon to sustain growth and efficient growth is dependent upon the sequential deglycosylation of the glycoconjugate substrate by pneumococcal exoglycosidases NanA, BgaA, and StrH. The second pneumococcal neuraminidase, NanB, is involved in the deglycosylation of host glycoconjugates and NanB activity can partially compensate for the loss or dysfunction of NanA
-
-
-
additional information
?
-
-
the induction of hST3Gal V, which synthesizes ganglioside GM3 and reduction of Neu3 by phorbol 12-myristate 13-acetate, are linked for the expression of differentiation marker protein, CD41b surface antigen. Neu3 overexpression inhibits the PMA-induced ERK1/2 and p38 MAPK phosphorylation in the K-562 cells. Down-regulation of expression of CD41b surface antigen is dependent on expression of Neu3 gene. Neu3 inhibitor 2-deoxy-2,3-didehydro-D-N-acetylneuraminic acid induces morphological changes, showing megakaryocytic differentiation of K-562 cells, with expression of CD41b surface antigen, while specific glucosylceramide synthase inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol inhibits megakaryocytic differentiation of K-562 cells
-
-
-
additional information
?
-
-
in addition to hydrolysis, enzyme catalyzes transglycosylation of sialyl residues to lactose. Km value and kcat value for sialyl transfer from 4-nitrophenyl alpha-sialoside are 5.8 mM and 6.77 per s, for sialyl transfer from (trifluoromethyl)umbelliferyl alpha-sialoside 0.26 mM and 15.7 per s, respectively
-
-
-
additional information
?
-
-
presence of Clostridium perfringens neuraminidase up-regulates expression of interleukin-8 mRNA and protein in lung epithelial A459 and NCI-H292 cells. Enzyme significantly up-regulates IL-8 promoter activity as well as nuclear factor-kappaB reporter activity. Inhibition of nuclear factor-kappaB signaling suppresses interleukin-8 mRNA expression induced by the neuraminidase
-
-
-
additional information
?
-
-
endoNF is therefore an inverting glycosidase, does not hydrolyze the monomeric and dimeric sialic acid with alpha-linked trifluoromethylumbelliferyl significantly over a 2 h period
-
-
-
additional information
?
-
-
no cleavage of 4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside and 4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside
-
-
-
additional information
?
-
-
trans-sialidase preferentially transfers sialic acid from sialyl-glycoconjugates to the terminal beta-galactosyl residues in acceptor molecules
-
-
-
additional information
?
-
-
both alpha2-3- and alpha2-6-linked sialosides are suitable substrates for NEU2 although it cleaves alpha2-3-linked sialosides more efficiently. Twenty 4-nitrophenyl-tagged alpha(2,3)- or alpha(2,6)-linked sialyl galactosides containing different terminal sialic acid forms including common N-acetylneuraminic acid, non-human N-glycolylneuraminic acid, 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid, or their C5-derivatives are used as substrates, overview
-
-
-
additional information
?
-
-
neuraminidase substrate specificity study of human and avian influenza A viruses, using a library of twenty alpha23- or alpha26-linked para-nitrophenol-tagged sialylgalactosides and thirty-seven strains of human and avian influenza A viruses, overview. Neuraminidases of all viruses tested cleave both alpha2-3- and alpha2-6-linked sialosides but prefer alpha23-linked ones and the activity is dependent on the terminal sialic acid structure
-
-
-
additional information
?
-
-
substrate recognition of the membrane-associated sialidase NEU3 requires a hydrophobic aglycone, two-site model for enzyme recognition, requiring interaction at both the Neu5Ac residue and the hydrophobic aglycone. Evaluation of series of 11 synthetic trisaccharides. NEU3 substrate activity is directly dependent upon the hydrophobicity of the aglycone but has no apparent requirement for features of the ceramide headgroup. Trisaccharides with incorporated azide groups in the Neu5Ac residue at either C9 or the N5-Ac position are substrates, and in the case of the N5-azidoacetyl derivative, the activity is superior to that of GM3. Incorporation of larger aryl groups is tolerated only at C9, but not at N5-Ac
-
-
-
additional information
?
-
-
substrate specificity of the viral enzyme with sialic acid, methyl 3'-sialyllactoside, methyl 6'-sialyllactoside, ligand conformations, enzyme-ligand interactions, and loop flexibility, analyzed by molecular docking and molecular dynamics simulations, and molecular modeling, overview. Methyl 3'-sialyllactoside has only weak interactions with the 150-loop, whereas the enzyme N1-methyl 6'-sialyllactoside complex shows strong interactions. The avian neuraminidase N1 preferentially cleaves sialic acid from alpha-(2-3)-Gal glycoconjugates over alpha-(2-6)-Gal, also due to the altered flexibility of loops in and around the active site
-
-
-
additional information
?
-
-
the enzyme catalyses the alpha-(2,3)-sialylation of non-reducing terminal beta-galactose residues, it is largely intolerant of substitution of the galactose 2 and 4 positions whereas substitution of the galactose 6 position is well tolerated, even the addition of a bulky sugar residue, glucose or galactose, does not impact negatively on TcTS binding and turnover, which highlights the potential of internal 6-substituted galactose residues to serve as enzyme acceptor substrates, substrate binding mode and site, overview
-
-
-
additional information
?
-
-
the key catalytic residues of the enzyme consist of the general acid-base D50 and the nucleophilic Y370-E225 pair, molecular modeling to predict residues involved in the recognition and hydrolysis of glycolipid substrates, homology modeling and molecular docking of NEU3, overview
-
-
-
additional information
?
-
-
the sialidase prefers alpha2-3-linked sialic acids over the alpha2-6 isomers. The enzyme shows no trans-sialidase activity. The purified sialidase releases sialic acid from diverse substrates such as mucin, fetuin, epithelial cell glycans and colominic acid, no activity with asialofetuin
-
-
-
additional information
?
-
Q7YZT3
trans-sialidase 1 produces alpha2,3-sialyllactose from lactose by desialylating fetuin exhibiting transsialidase activity, substrate specificities for lactose, fetuin and synthetic substrates, overview. The enzyme is able to sialylate asialofetuin. Sialylation pattern of the N-glycans on fetuin catalyzed by the trans-sialidases, overview
-
-
-
additional information
?
-
-
sialidase NEU3 shows high enzymatic specificity toward gangliosides
-
-
-
additional information
?
-
A0A024FS29, A0A024FSF5, I3JCG9
isoform Neu3a shows no activity with ganglioside GM1
-
-
-
additional information
?
-
L0N7M7
no activity with mucin, colominic acid, gangliosides and fetuin
-
-
-
additional information
?
-
A0A024FS29, A0A024FSF5, I3JCG9
sialidase activities are not observed towards various sialoglycoconjugates
-
-
-
additional information
?
-
-
siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages
-
-
-
additional information
?
-
-
the enzyme shows higher activity on alpha2,3-linked sialic acid than alpha2,6-linked
-
-
-
additional information
?
-
-
the strict hydrolase of Trypanosoma rangeli shows no trans-sialidase activity
-
-
-
additional information
?
-
Trypanosoma vivax Agai
-
the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI
-
-
-
additional information
?
-
-
the sialidase prefers alpha2-3-linked sialic acids over the alpha2-6 isomers. The enzyme shows no trans-sialidase activity. The purified sialidase releases sialic acid from diverse substrates such as mucin, fetuin, epithelial cell glycans and colominic acid, no activity with asialofetuin
-
-
-
additional information
?
-
Trypanosoma vivax Agari
-
the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI
-
-
-
additional information
?
-
Clostridium chauvoei Jakari
-
the enzyme cleaves sialic acids from RBC ghosts of sheep, horse, goat, cattle, pig and mice as well as mouse brain cells
-
-
-
additional information
?
-
influenza A virus H5N1
-
the enzyme shows higher activity on alpha2,3-linked sialic acid than alpha2,6-linked
-
-
-
additional information
?
-
influenza A virus H5N1
-
substrate specificity of the viral enzyme with sialic acid, methyl 3'-sialyllactoside, methyl 6'-sialyllactoside, ligand conformations, enzyme-ligand interactions, and loop flexibility, analyzed by molecular docking and molecular dynamics simulations, and molecular modeling, overview. Methyl 3'-sialyllactoside has only weak interactions with the 150-loop, whereas the enzyme N1-methyl 6'-sialyllactoside complex shows strong interactions. The avian neuraminidase N1 preferentially cleaves sialic acid from alpha-(2-3)-Gal glycoconjugates over alpha-(2-6)-Gal, also due to the altered flexibility of loops in and around the active site
-
-
-
additional information
?
-
Clostridium perfringens CN3718
-
siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages
-
-
-
additional information
?
-
Clostridium sordellii G12
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification, enzyme is responsible for decomposition of organic material in various biotopes
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
?
show the reaction diagram
-
-
-
-
?
alpha(2-3)-linked sialic acid + H2O
?
show the reaction diagram
-
the enzyme is selective for alpha(2-3)-linked sialic acid
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha(2-3)sialyl-lactose + H2O
?
show the reaction diagram
-
-
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
show the reaction diagram
-
-
-
-
?
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
-
bovine brain gangliosides + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
bovine submaxillary gland mucin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
colominic acid + H2O
?
show the reaction diagram
-
-
-
-
-
colominic acid + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
-
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus
R4JGE3, R4RZI6
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
sialic acid + ?
show the reaction diagram
Q80A54, Q80A58
-
-
-
?
fetuin + H2O
?
show the reaction diagram
influenza A virus H1N1
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus A/Shanghai/1/2013
R4RZI6
-
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fetuin + H2O
?
show the reaction diagram
H7N9 influenza virus A/Anhui/1/2013
R4JGE3
-
-
-
?
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
-
ganglioside GD1a + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
show the reaction diagram
-
i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
show the reaction diagram
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
ganglioside GM1 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
ganglioside GM3 + H2O
sialic acid + ?
show the reaction diagram
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
lactosyl-ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
?
show the reaction diagram
-
-
-
-
-
orosomucoid + H2O
sialic acid + ?
show the reaction diagram
-
-
-
-
?
polysialic acid + H2O
sialic acid
show the reaction diagram
-
-
-
-
?
monosialoganglioside GM3 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme is involved in the metabolism of gangliosides
-
-
-
additional information
?
-
-
enzyme is required for cellular signaling and for the production of cytokine interleukin-4 by activated T-lymphocytes, inherited enzyme defiency results in sialidosis or galactosialidosis, both severe metaboic disorders associated with lysosomal storage of oligosaccharides and glycopeptides
-
-
-
additional information
?
-
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification, enzyme is responsible for decomposition of organic material in various biotopes
-
-
-
additional information
?
-
-
essential enzyme, enzyme containing culture filtrate possesses the ability to aggluinate and virus and shows receptor properties for erythrocytes, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
-
essential enzyme, enzyme containing culture filtrate possesses the ability to agglutinate and virus and shows receptor properties for erythrocytes, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification
-
-
-
additional information
?
-
Q9EZV6, Q9EZV7
most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an actiev infection
-
-
-
additional information
?
-
Q9EZV6, Q9EZV7
most strains of Pasteurella multocida produce sialidase activity which may contribute to colonization of the respiratory tract by the mucosal pathogen, or for production of lesions in an active infection
-
-
-
additional information
?
-
-
the enzyme modifies the ganglioside pattern of adjacent cells, involved in cell-cell-interaction
-
-
-
additional information
?
-
Q54727
NanB may contribute to Strreptococcus pneumoniae colonization and pathogenesis
-
-
-
additional information
?
-
-
sialidase-3 is a key enzyme for ganglioside hydrolysis. Neu3 is an important regulator of insulin sensitivity and glucose tolerance
-
-
-
additional information
?
-
-
the Neu3 gene represents a physiological modulator of vascular smooth muscle cells responses that may contribute to plaque instability in atherosclerosis
-
-
-
additional information
?
-
-
increased CD15 and decreased CD15s expression during myeloid differentiation is sialidase dependent. Both CD15s synthesis and sialidase activity are induced by CD44 ligation, but the increase in sialidase activity dominates, such that the overall expression pattern upon CD44 ligation is a decrease in CD15s expression with an accompanying increase in CD15. The observed increase in CD15 results predominantly from conversion of CD15s to CD15 on glycoproteins
-
-
-
additional information
?
-
-
neuraminidase causes the desialylation of both PDGF and IGF-1 receptors and diminishes the intracellular signals induced by the mitogenic ligands PDGF-BB and IGF-2
-
-
-
additional information
?
-
Q54727
Streptococcus pneumoniae is able to utilize complex N-linked human glycoconjugates as a sole source of carbon to sustain growth and efficient growth is dependent upon the sequential deglycosylation of the glycoconjugate substrate by pneumococcal exoglycosidases NanA, BgaA, and StrH. The second pneumococcal neuraminidase, NanB, is involved in the deglycosylation of host glycoconjugates and NanB activity can partially compensate for the loss or dysfunction of NanA
-
-
-
additional information
?
-
-
the induction of hST3Gal V, which synthesizes ganglioside GM3 and reduction of Neu3 by phorbol 12-myristate 13-acetate, are linked for the expression of differentiation marker protein, CD41b surface antigen. Neu3 overexpression inhibits the PMA-induced ERK1/2 and p38 MAPK phosphorylation in the K-562 cells. Down-regulation of expression of CD41b surface antigen is dependent on expression of Neu3 gene. Neu3 inhibitor 2-deoxy-2,3-didehydro-D-N-acetylneuraminic acid induces morphological changes, showing megakaryocytic differentiation of K-562 cells, with expression of CD41b surface antigen, while specific glucosylceramide synthase inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol inhibits megakaryocytic differentiation of K-562 cells
-
-
-
additional information
?
-
-
endoNF is therefore an inverting glycosidase
-
-
-
additional information
?
-
-
sialidase NEU3 shows high enzymatic specificity toward gangliosides
-
-
-
additional information
?
-
Clostridium sordellii G12
-
essential enzyme, bacterial enzymes may serve as a colonization and virulence factor or as an important tool for nutrification, enzyme is responsible for decomposition of organic material in various biotopes
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ba2+
-
stimulates the activity of the enzyme
Ba2+
-
stimulates the activity of the enzyme
Br-
-
activates
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
-
stimulates the activity of the enzyme
Ca2+
Q80A54, Q80A58
-
Ca2+
-
required
Ca2+
-
required
Ca2+
-
required for activity
Ca2+
-
activates
Ca2+
-
activates about 2fold, increase in initial reaction velocity, required
Ca2+
-
activates
Ca2+
-
activates
Ca2+
-
about 170% activity at 1 mM
Ca2+
Q8AAK9
the activity is maintained more than 94% in the presence of Ca2+ at concentrations up to 5 mM
Ca2+
-
contains Ca2+
Cl-
-
activates up to 1 M
Cl-
-
activates up to 100 mM, above 100 mM chloride inhibits the enzyme
Co2+
-
stimulates the activity of the enzyme
Co2+
-
stimulates the activity of the enzyme
Co2+
-
110% activity at 10 mM
Cu2+
-
complete inhibition at 1 mM
Fe2+
-
stimulates the activity of the enzyme
Hg2+
-
complete inhibition at 1 mM
K+
-
stimulates the activity of the enzyme
K+
-
slight activation up to 0.01 mM
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
-
stimulates the activity of the enzyme
Mg2+
Q80A54, Q80A58
-
Mg2+
-
activates about 2fold, increase in initial reaction velocity, required
Mg2+
-
activates
Mg2+
-
activates
Mg2+
-
activates
Mg2+
-
about 150% activity at 1 mM
Mg2+
-
required
Mg2+
-
115% activity at 10 mM
Mg2+
Q8AAK9
the activity is maintained more than 96% in the presence of Mg2+ at concentrations up to 5 mM
Mn2+
-
stimulates the activity of the enzyme
Mn2+
-
stimulates the activity of the enzyme
Mn2+
-
stimulates the activity of the enzyme
Mn2+
-
stimulates the activity of the enzyme
Mn2+
-
stimulates the activity of the enzyme
Mn2+
-
110% activity at 10 mM
Na+
-
slight activation up to 0.01 mM
Ni2+
Q8AAK9
the activity is maintained more than 75% in the presence of Ni2+ at concentrations up to 5 mM
NO3-
-
activates
S2O42-
-
activates
SO32-
-
activates
SO42-
-
activates
Mn2+
Q8AAK9
the activity is maintained more than 69% in the presence of Mn2+ at concentrations up to 5 mM
additional information
-
not affected by nitrate, sulfate, sulfite, and disulfite ions
additional information
-
decrease in activity through removal of divalent cations results in decreased immunogenicity, antibody response decreases by about 68-76%, 80% of the original activity is restored by addition of Ca2+ and Mg2+
additional information
-
CaCl2, MgCl2 or MnCl2 do not increase enzyme activity
additional information
-
not influenced by Fe2+ and Ni2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1R/S)-1-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-propan-1-ol
-
13% inhibition at 1 mM
(1R/S)-1-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-propan-1-ol
-
29% inhibition at 1 mM
(1R/S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-(4-phenethyl-[1,2,3]triazol-1-yl)cyclohex-2-ene-1-carboxylic acid
-
-
(1R/S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxy-1-methylethyl)[1,2,3]triazol-1-yl]cyclohex-2-ene-1-carboxylic acid
-
-
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(acetylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(benzoylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(butyrylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-[(cyclopropylcarbonyl)amino]-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-ethoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-hydroxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-methoxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
i.e. A-315675
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybutyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
(2R/S)-1-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-propan-2-ol
-
14% inhibition at 1 mM
(2R/S)-1-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-propan-2-ol
-
10% inhibition at 1 mM
(3E)-3-(1,3-benzothiazol-2-yl)-4-(2,5-dimethylphenyl)but-3-enoate
-
-
(3E)-3-(1,3-benzothiazol-2-yl)-4-(2-ethoxyphenyl)but-3-enoate
-
-
(3E)-3-(1,3-benzothiazol-2-yl)-4-(3,4-dimethoxyphenyl)but-3-enoate
-
-
(3E)-3-(1,3-benzothiazol-2-yl)-4-(3-fluorophenyl)but-3-enoate
-
-
(3E)-3-(1,3-benzothiazol-2-yl)-4-[2-(propan-2-yloxy)phenyl]but-3-enoate
-
-
(3E)-3-(1,3-benzothiazol-2-yl)-4-[2-methyl-5-(trifluoromethyl)phenyl]but-3-enoate
-
-
(3R,4R,5R)-4-acetamido-3-(1-(diaminomethylamino)-3-hydroxypropan-2-yloxy)-5-((1S)-1,2,3-trihydroxypropyl)cyclohex-1-enecarboxylic acid
-
obtained by structure-based design using crystal structure PDB ID 2hty and in order to exploit experimentally identified potential benefits offered by the 150-cavity adjacent to the H5N1 neuramindase active site. Inhibitor shows low binding free energy
(3R,4R,5R)-4-acetamido-5-((1S)-2-carboxy-1,2-dihydroxyethyl)-3-(1-(diaminomethylamino)-3-hydroxypropan-2-yloxy)cyclohex-1-enecarboxylic acid
-
obtained by structure-based design using crystal structure PDB ID 2hty and in order to exploit experimentally identified potential benefits offered by the 150-cavity adjacent to the H5N1 neuramindase active site. Inhibitor shows low binding free energy
(3S,4R,5R)-4-acetamido-3-amino-5-(1-ethylpropoxy)cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-3-[4-((17alpha)-estra-1,3,5(10)-triene-3,17-dihydroxy-17-yl)[1,2,3]triazol-1-yl]-5-(1-ethylpropoxy)cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethyl-propoxy)-3-[4-(1-hydroxypropyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-(4-phenethyl-[1,2,3]triazol-1-yl)cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-guanidinocyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxy-1-methylethyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxycyclohexyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxycyclopentyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
-
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(3-hydroxypropyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
-
(4R)-2-(2-carboxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-carboxyphenyl)-3-(chloroacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-carboxyphenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-carboxyphenyl)-3-glycyl-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-hydroxy-3-methoxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-hydroxy-3-methoxyphenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-hydroxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-hydroxyphenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-nitrophenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(2-nitrophenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(4-cyanophenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(4-cyanophenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(4-cyanophenyl)-3-glycyl-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(furan-2-yl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(furan-2-yl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-(furan-2-yl)-3-glycyl-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-phenyl-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-2-phenyl-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-(2-hydroxy-3-methoxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-(2-hydroxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-(2-nitrophenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-(4-cyanophenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-(furan-2-yl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-(chloroacetyl)-2-phenyl-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-glycyl-2-(2-hydroxy-3-methoxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-glycyl-2-(2-hydroxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-glycyl-2-(2-nitrophenyl)-1,3-thiazolidine-4-carboxylic acid
-
-
(4R)-3-glycyl-2-phenyl-1,3-thiazolidine-4-carboxylic acid
-
-
(6aS,12aS)-6a,12a-dihydro-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-3-ol
-
noncompetitive
(6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid
-
competitive
1,3,6,7-tetrahydroxy-2-(3-methylbut-2-enyl)-8-(2-methylbut-3-en-2-yl)-9H-xanthen-9-one
Q59310
competitive
1,3,8-trihydroxy-6-methylanthracene-9,10-dione
-
-
1,3-bis[1-[(beta-D-galactopyranosyl)]-1H-1,2,3-triazol-6-yl]-propane
-
8% inhibition at 1 mM
1,3-bis{1-[(methyl 6-deoxy-beta-D-galactopyranos-6-yl)]-1H-1,2,3-triazol-6-yl}-propane
-
16% inhibition at 1 mM
1,8-dihydroxyanthracene-9,10-dione
-
-
1-(beta-D-galactopyranosyl)-1H-1,2,3-[triazol-4-yl]-methanol
-
11% inhibition at 1 mM
11,17-dihydroxy-3,20-dioxopregn-4-en-21-yl cyclopentanecarboxylate
-
identified by ensemble-based virtual screening
2'-methoxyisoliquiritigenin
-
noncompetitive inhibition
2,3 didehydroneuraminic acid
-
-
2,3-dehydro-2-deoxy-N-acetylneuraminic acid
-
treatment of cultured human aortic smooth muscle cells induces significant up-regulation in smooth muscle cell proliferation in response to fetal bovine serum
2,3-dehydro-2-deoxy-N-acetylneuraminic acid
-
-
2,3-dehydro-2-deoxy-N-acetylneuraminic acid
-
33% inhibition at 1 mM
2,3-dehydro-2-deoxy-N-acetylneuraminic acid
-
-
2,3-dehydro-2-deoxy-N-acetylneuraminic acid
-
competitive inhibitor; competitive inhibitor
2,3-dehydro-2-sialic acid
-
70.2% inhibition at 0.1 mM
-
2,3-didehydro-2-deoxy-N-acetylneuraminic acid
-
i.e. DANA, specific, competitive neuraminidase inhibitor
2,3-didehydro-N-acetylneuraminic acid
-
-
2,3-didehydroneuraminic acid
-
-
2,3-didehydroneuraminic acid
-
competitive
2,4-diaminohexahydropyrimidine-5-carbonitrile
-
identified by ensemble-based virtual screening
2,6-anhydro-3,5-dideoxy-5-glycolylamido-D-glycero-D-galactonon-2-enonic acid
-
-
2,6-anhydro-3-deoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
2,6-anhydro-5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
2,6-anhydro-9-azido-3,5,9-trideoxy-5-(2-hydroxyacetamido)-D-glycero-D-galacto-non-2-enonic acid
-
-
-
2,6-anhydro-9-azido-3,5,9-trideoxy-5-(2-hydroxyacetamido)-D-glycero-D-galacto-non-2-enonic acid
P29768
-
-
2,6-anhydro-9-azido-3,5,9-trideoxy-5-(2-hydroxyacetamido)-D-glycero-D-galacto-non-2-enonic acid
-
-
-
2,6-anhydro-9-azido-5-(2-azidoacetamido)-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
2,6-anhydro-9-azido-5-(2-azidoacetamido)-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
P29768
-
-
2,6-anhydro-9-azido-5-(2-azidoacetamido)-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
2-((E)-4'-hydroxyphenylidene)-4,6-dihydroxy-2,3-dihydrobenzofuran-3-one
-
-
2-((E)-4'-hydroxyphenylidene)-6-hydroxy-2,3-dihydrobenzofuran-3-one
-
-
2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-3-(4-hydroxy-3-nitrophenyl)propanoic acid
-
identified by ensemble-based virtual screening
2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one
-
-
2-(3,4-dihydroxyphenyl)-4H-chromene-3,5,7-triol
-
-
2-(E-4'-hydroxyphenylidene)-4,6-dihydroxy-2,3-dihydrobenzo-furan-3-one
-
-
2-(E-4'-hydroxyphenylidene)-6-hydroxy-2,3-dihydrobenzofuran-3-one
-
-
2-(E-benzylidene)-6-hydroxy-2,3-dihydrobenzofuran-3-one
-
-
2-([4,5-dimethyl-3-(propoxycarbonyl)thiophen-2-yl]carbamoyl)cyclohexanecarboxylate
-
-
2-chloromercuribenzoate
-
18% residual activity at 10 mM
-
2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetyl neuraminic acid
P62575
-
2-deoxy-2,3-dehydro-N-acetyl neuraminic acid
Q8AAK9
competitive inhibition
2-deoxy-2,3-dehydro-N-acetyl-D-neuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetyl-D-neuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetyl-D-neuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
P29768
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
-
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
-
inhibition occurs in the micromolar range
2-deoxy-2,3-didehydro-D-glycero-D-galacto-2-nonulopyranosonic acid
-
-
2-deoxy-2,3-didehydro-D-N-acetylneuraminic acid
-
induces morphological changes, showing megakaryocytic differentiation of K-562 cells, with expression of CD41b surface antigen
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
i.e. NeuAc2,3en, competitive
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-aniline
-
30% inhibition at 1 mM
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-bromobenzene
-
33% inhibition at 1 mM
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-N-phthalimidylmethane
-
25% inhibition at 1 mM
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-phenylethane
-
16% inhibition at 1 mM
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-pyridine
-
13% inhibition at 1 mM
2-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
14% inhibition at 1 mM
2-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-aniline
-
24% inhibition at 1 mM
2-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-bromobenzene
-
29% inhibition at 1 mM
2-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-pyridine
-
14% inhibition at 1 mM
2-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
11% inhibition at 1 mM
2-methoxyisoliquiritigenin
-
-
3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one
-
-
3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
3-(2-amino-1-hydroxyethyl)-4-[(methylsulfonyl)amino]benzoic acid
-
1 mM, 17% inhibition
3-(acetylamino)-4-morpholin-4-ylbenzoic acid
-
-
3-amino-4-(benzylthio)benzoic acid
-
-
3-amino-4-(pyridin-3-ylamino)benzoic acid
-
-
3-amino-4-[(phenylacetyl)amino]benzoic acid
-
-
3-hydroxy-4-[(E)-naphthalen-1-yldiazenyl]naphthalene-2,7-disulfonic acid
-
identified by ensemble-based virtual screening
3-[(aminoacetyl)amino]-4-(benzylthio)benzoic acid
-
-
3-[(diaminomethylene)amino]-4-[(methylamino)carbonyl]benzoic acid
-
-
3-[(diaminomethylene)amino]-4-[(methylsulfinyl)amino]benzoic acid
-
1 mM, 29% inhibition
3-[(diaminomethylene)amino]-4-[(phenylacetyl)amino]benzoic acid
-
1 mM, 54% inhibition
3-[(diaminomethylene)amino]-5-(hydroxymethyl)-4-[(methylsulfonyl)amino]benzoic acid
-
1 mM, 11% inhibition
3-[(diaminomethylene)amino]-5-[(E)-(hydroxyimino)methyl]-4-[(methylsulfonyl)amino]benzoic acid
-
1 mM, 46% inhibition
3-[(diaminomethylene)amino]-5-[(E)-(hydroxyimino)methyl]benzoic acid
-
1 mM, 35% inhibition
3-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-propan-1-ol
-
27% inhibition at 1 mM
3-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-pyridine
-
15% inhibition at 1 mM
3-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
31% inhibition at 1 mM
3-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-propan-1-ol
-
24% inhibition at 1 mM
3-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-pyridine
-
20% inhibition at 1 mM
3-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
3% inhibition at 1 mM
4,5-diacetamido-2-fluorobenzoate
-
8% inhibition of isoform NEU4 at 1 mM
4,5-diacetamido-2-fluorobenzoic acid
-
23% inhibition of isoform NEU3 at 1 mM; 37% inhibition of isoform NEU2 at 1 mM
4,5-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-carboxylic acid
-
-
4-(acetylamino)-3,5-bis(guanidino)-2-ethoxybenzoic acid
-
-
4-(acetylamino)-3-(2,3-dihydroxypropyl)benzoic acid
-
1 mM, 17% inhibition
4-(acetylamino)-3-(2-aminoethyl)benzoic acid
-
1 mM, 25% inhibition
4-(acetylamino)-3-(2-hydroxyethyl)benzoic acid
-
1 mM, 16% inhibition
4-(acetylamino)-3-(hydroxymethyl)benzoic acid
-
-
4-(acetylamino)-3-[(diaminomethylene)amino]benzoic acid
-
1 mM, 41% inhibition
4-(acetylamino)-3-[(Z)-(hydroxyimino)methyl]benzoic acid
-
1 mM, 24% inhibition
4-(acetylamino)-5-(guanidino)-2-(cyclopentyloxy)benzoic acid
-
-
4-(acetylamino)-5-(guanidino)-2-butoxybenzoic acid
-
-
4-(acetylamino)-5-(guanidino)-2-isopropoxybenzoic acid
-
-
4-(benzylamino)-3-[(diaminomethylene)amino]benzoic acid
-
1 mM, 9% inhibition
4-(benzylthio)-3-[(diaminomethylene)amino]benzoic acid
-
1 mM, 43% inhibition
4-acetamido-5-(2-ethylbutanamido)-2-fluorobenzoic acid
-
23% inhibition of isoform NEU2 at 1 mM; less than 5% inhibition of isoform NEU4 at 1 mM
4-acetamido-5-(2-methylbutanamido)-2-fluorobenzoic acid
-
7% inhibition of isoform NEU2 at 1 mM
4-acetamido-5-(2-naphthamido)-2-fluorobenzoic acid
-
10% inhibition of isoform NEU4 at 1 mM; 33% inhibition of isoform NEU2 at 1 mM; 8% inhibition of isoform NEU3 at 1 mM
4-acetamido-5-(biphenyl-4-ylcarboxamido)-2-fluorobenzoic acid
-
10% inhibition of isoform NEU4 at 1 mM; 60% inhibition of isoform NEU2 at 1 mM; 8% inhibition of isoform NEU3 at 1 mM
4-acetamido-5-benzamido-2-fluorobenzoic acid
-
17% inhibition of isoform NEU2 at 1 mM
4-acetamido-5-cyclohexanecarboxamido-2-fluorobenzoic acid
-
17% inhibition of isoform NEU2 at 1 mM; 6% inhibition of isoform NEU4 at 1 mM
4-acetamido-5-cyclopropanecarboxamido-2-fluorobenzoic acid
-
20% inhibition of isoform NEU2 at 1 mM; less than 5% inhibition of isoform NEU4 at 1 mM
4-acetamido-5-isobutyramido-2-fluorobenzoic acid
-
11% inhibition of isoform NEU4 at 1 mM; 15% inhibition of isoform NEU2 at 1 mM
4-acetamido-5-pentanamido-2-fluorobenzoic acid
-
13% inhibition of isoform NEU3 at 1 mM; 25% inhibition of isoform NEU2 at 1 mM; less than 5% inhibition of isoform NEU4 at 1 mM
4-acetamido-5-pivalamido-2-fluorobenzoic acid
-
less than 5% inhibition of isoform NEU4 at 1 mM
4-aminophenyl thioglucoside derivatives of sialic acid
-
-
-
4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid
human parainfluenza virus
-
i.e. BCX 2798. Effectively inhibits the activities of the hemagglutinin-neuraminidase of human parainfluenza viruses in vitro and protects mice from lethal infection with a recombinant Sendai virus whose hemagglutinin-neuraminidase was replaced with that of human parainfluenza virus-1
4-hydroxymercuribenzoate
-
-
4-hydroxymercuribenzoate
-
cytosolic and intralysosomal sialidase, not membrane-associated sialidases I and II
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
substrate inhibition
4-nitrophenyl oxamic acid
-
-
4-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-aniline
-
19% inhibition at 1 mM
4-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-butan-1-ol
-
26% inhibition at 1 mM
4-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-butanoic acid
-
10% inhibition at 1 mM
4-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
9% inhibition at 1 mM
4-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-aniline
-
34% inhibition at 1 mM
4-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-bromobenzene
-
3% inhibition at 1 mM
4-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-butan-1-ol
-
27% inhibition at 1 mM
4-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-trifluoromethylbenzene
-
10% inhibition at 1 mM
5,6,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one
-
-
5,7,4'-trihydroxy-8-methoxyflavone
-
-
5,7-dihydroxy-2-(2-hydroxyphenyl)-4H-chromen-4-one
-
-
5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
5,7-dihydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one
-
-
5,7-dihydroxy-2-phenyl-4H-chromen-4-one
-
-
5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
5-(acetylamino)-2,6-anhydro-3,5,9-trideoxy-9-(pentanoylamino)-L-glycero-L-altro-non-2-enonic acid
-
-
5-(acetylamino)-2,6-anhydro-3,5,9-trideoxy-9-[(3-methylbutanoyl)amino]-L-glycero-L-altro-non-2-enonic acid
-
-
5-(acetylamino)-2,6-anhydro-9-[(cyclopentylcarbonyl)amino]-3,5,9-trideoxy-L-glycero-L-altro-non-2-enonic acid
-
-
5-(acetylamino)-4-aminopyridine-2-carboxylic acid
-
1 mM, 14% inhibition
5-(acetylamino)-6-aminopyridine-2-carboxylic acid
-
-
5-(acetylamino)pyridine-2-carboxylic acid
-
1 mM, 22% inhibition
5-(ethanethioylamino)pyridine-2-carboxylic acid
-
-
5-acetamido-2,6-anhydro-3,5-dideoxy-9-O-methyl-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-acetamido-2,6-anhydro-3,5-dideoxy-9-O-methyl-D-glycero-D-galacto-non-2-enonic acid
P29768
-
-
5-acetamido-2,6-anhydro-3,5-dideoxy-9-O-methyl-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-acetamido-2,6-anhydro-3,5-dideoxy-D-glycero-D-galactonon-2-enonic acid
-
-
5-acetamido-2,6-anhydro-9-azido-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-acetamido-2,6-anhydro-9-azido-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
P29768
-
-
5-acetamido-2,6-anhydro-9-azido-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-acetamido-2-(4-N-5-dimethylaminonaphthalene-1-sulfonyl-2-difluoromethylphenyl)3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosonic acid
-
irreversible and competitive
5-acetamido-9-aminopropyl-2,6-anhydro-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-acetamido-9-aminopropyl-2,6-anhydro-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
P29768
-
-
5-acetamido-9-aminopropyl-2,6-anhydro-3,5,9-trideoxy-D-glycero-D-galacto-non-2-enonic acid
-
-
-
5-hydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one
-
-
5-methyl-4-methylidene-2-phenyl-4H-chromene
-
-
5-[(methylsulfonyl)amino]pyridine-2-carboxylic acid
-
1 mM, 25% inhibition
6,6-dimethyl-1-(3-[(3-nitrobenzyl)oxy]phenyl)-1,3,5-triazinane-2,4-diimine
-
identified by ensemble-based virtual screening
6-chloro-9,10-dihydro-4,5,7-trihydroxy-9,10-dioxo-2-anthracenecarboxylic acid
-
-
6-chloro-9,10-dihydro-4,5,7-trihydroxy-9,10-dioxo-2-anthracenecarboxylic acid
-
strong inhibitor
7,4'-di-O-galloyltricetinflavan
-
-
7-hydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
-
7-O-galloyltricetinflavan
-
-
9,10-dioxo-9,10-dihydroanthracene-2-carboxylic acid
-
-
alpha-D-N-acetylneuraminic acid
-
-
alpha-D-N-acetylneuraminic acid
-
-
alpha-D-N-acetylneuraminic acid
-
-
apigenin
-
-
asteropine A
-
a conotoxin-like peptide from the marine sponge Asteropus simplex, competitive inhibition
-
Berberine
-
reversible non-competitive inhibition
Ca2+
-
-
Ca2+
-
above 1 mM
Ca2+
-
-
Ca2+
-
90% residual activity at 10 mM
calopocarpin
-
noncompetitive inhibition
Cd2+
-
-
chondroitin sulfate A
-
-
CID2601775
-
the IUPAC name is 2-[(4-amino-5-cyclopropyl-1,2,4-triazol-3-yl)sulfanyl]-N-(cyclopentylcarbamoyl)acetamide
CID9796290
-
the IUPAC name is 6-[2-[[2-[(2S)-2-cyanopyrrolidin-1-yl]-2-oxoethyl]amino]ethylamino]pyridine-3-carbonitrile
Cl-
-
recombinant mutant: increase in concentration from 5 to 150 mM results in a decrease in activity of 50%, native enzyme: increase in concentration from 10 to 150 mM results in a decrease in activity of 20%
Co2+
-
-
Co2+
-
-
cristacarpin
-
noncompetitive inhibition
Cu2+
-
-
Cu2+
-
cytosolic and intralysosomal sialidase, not membrane-associated sialidases I and II
Cu2+
-
above 0.01 mM
Cu2+
-
about 40% residual activity at 1 mM
Cu2+
-
95% inhibition at 10 mM
Cu2+
Q8AAK9
2.9% residual activity at 5 mM
cudratricusxanthone
Q59310
competitive
cudraxanthone L
Q59310
competitive
cudraxanthone M
Q59310
competitive
D-glucosamine 6-sulfate
-
slight inhibition at 10 mM
D-glucose 6-sulfate
-
slight inhibition at 10 mM
daidzein
-
-
dehydrocorybulbine
-
-
-
demethylmedicarpin
-
noncompetitive inhibition
Dextran sulfate
-
-
-
diethyldicarbonate
-
-
dihydroisoquinoline derivative
-
-
dithiothreitol
-
-
dithiothreitol
-
-
EDTA
-
-
EDTA
-
20% inhibition at 1 mM, 54% at 10 mM
erystagallin A
-
noncompetitive inhibition
erysubin D
-
competitive inhibitor
erysubin E
-
noncompetitive inhibition
erythribyssin D
-
i.e. 3-hydroxy-[2',2'-(3'-hydroxy)-dimethylpyrano]-(5',6':10,9)-(6aR,11aR)pterocarpan
erythribyssin L
-
i.e. 9-hydroxy-10-prenyl-[2',2'-(3'-hydroxy)-dimethylpyrano]-(5',6':2,3)-(6aR,11aR)pterocarpan
erythribyssin M
-
3-hydroxy-[2',2'-(3'-hydroxy)-dimethylpyrano]-(5',6':10,9)-(6aS,11aS)pterocarpan
erythribyssin O
-
noncompetitive inhibition
eryvarin D
-
noncompetitive inhibition
Fe2+
-
about 10% residual activity at 1 mM
Fe2+
Q8AAK9
33.7% residual activity at 5 mM
Fe3+
-
-
Fe3+
-
56% residual activity at 10 mM
Galp(beta1,2)Galf(beta1,4)GlcNAc
-
-
Galp(beta1,2)Galf(beta1,4)GlcNAcOBn
-
-
Galp(beta1,2)Galf(beta1,4)GlcNAcol
-
-
Galp(beta1,2)[Galp(beta1,3)]Gal
-
-
Galp(beta1,2)[Galp(beta1,3)]GalOBn
-
-
Galp(beta1,2)[Galp(beta1,3)]Galol
-
-
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
Galp(beta1,6)GlcNAc
-
-
Galp(beta1,6)GlcNAcOBn
-
-
Galp(beta1,6)GlcNAcol
-
-
Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
genistein
-
-
ginkgetin
-
-
glyasperin C
-
20% inhibition at 0.2 mM
glyasperin D
-
20% inhibition at 0.2 mM
glycyrin
-
10% inhibition at 0.2 mM
glycyrol
-
strong inhibitory activity, noncompetitive inhibition
Hg2+
-
-
Hg2+
-
high concentrations, 50% inhibition at 5.2 mM
hinokiflavone
-
-
hinokiflavone-sialic acid
-
-
hispidulin
-
-
iodoacetamide
-
-
isoglycyrol
-
noncompetitive inhibition
isoliquiritigenin
-
strong inhibitory activity, noncompetitive inhibition
isoliquiritin
-
noncompetitive inhibition
isoliquiritin apioside
-
noncompetitive inhibition
isoneorautenol
-
noncompetitive inhibition
Jatrorrhizine
-
-
K+
-
above 1 mM
kaempferol
-
-
kaempferol 3-O-beta-xylopyranosyl-(1->2)-beta-D-glucopyranoside
-
-
kumatakenin
-
noncompetitive inhibition
L-Cysteine hydrochloride
-
-
laninamivir
-
-
-
Li+
-
9% inhibition at 10 mM
licocoumarone
-
noncompetitive inhibition
licoflavonol
-
noncompetitive inhibition
licopyranocoumarin
-
10% inhibition at 0.2 mM
licorisoflavan A
-
30% inhibition at 0.2 mM
liquiritigenin
-
noncompetitive inhibition
liquiritin
-
noncompetitive inhibition
liquiritin apioside
-
noncompetitive inhibition
lung surfactant protein A
-
lung surfactant protein D has greater neuraminidase inhibitory activity than mannose-binding lectin, which in turn has greater activity than lung surfactant protein A
-
lung surfactant protein D
-
neuraminidase inhibition by lung surfactant protein D correlates with binding of its carbohydrate recognition domain to oligomannose oligosaccharides on the viral hemagglutinin. The effects of lung surfactant protein D are additive with oseltamivir. Neuraminidase inhibition is observed using fetuin or MDCK cells as a substrate, but not in assays using a soluble sialic acid analogue. Lung surfactant protein D has greater neuraminidase inhibitory activity than mannose-binding lectin, which in turn has greater activity than lung surfactant protein A
-
luteolin
-
-
luteolin 7-O-beta-D-glucopyranoside
-
-
macluraxanthone
Q59310
competitive
mannose-binding lectin
-
lung surfactant protein D has greater neuraminidase inhibitory activity than mannose-binding lectin, which in turn has greater activity than lung surfactant protein A
-
mercaptoethanol
-
-
methyl 4-(acetylamino)-3,5-bis(guanidino)-2-ethoxybenzoate
-
-
methyl 4-(acetylamino)-3-amino-5-hydroxycyclopent-1-ene-1-carboxylate
-
-
methyl 4-(acetylamino)-5-amino-2-(3-methylbutoxy)benzoate
-
-
methyl thioglucoside derivatives of sialic acid
-
-
-
methyl [1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-carboxylate
-
28% inhibition at 1 mM
methyl [1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-carboxylate
-
30% inhibition at 1 mM
Mg2+
-
-
Mg2+
-
-
myricetin
-
-
N-(4-nitrophenyl)-oxamic acid
-
-
N-(4-nitrophenyl)-oxamic acid
-
-
N-acetyl-2,3-dehydro-2-deoxyneuraminic acid
-
-
-
N-acetylneuraminic acid
-
-
N-benzyl-N-methyl-1-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-methylamine
-
22% inhibition at 1 mM
N-benzyl-N-methyl-1-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-methylamine
-
17% inhibition at 1 mM
N-bromosuccimide
-
-
N-bromosuccinimide
-
-
N-bromosuccinimide
-
strain L
N-bromosuccinimide
-
competitive
N-bromosuccinimide
-
-
N-ethylmaleimide
-
competitive
N-ethylmaleimide
-
-
Na+
-
above 1 mM
neorautenol
-
noncompetitive inhibition
Neu5Ac
-
i.e. alpha-D-N-acetylneuraminic acid, competitive, 17% inhibition at 2 mM, 20% inhibition at 5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Neu5Ac2en
-
inhibits competitively the hydrolysis of KDNalpha-(2-8)-KDN
Neu5Ac2en
-
i.e. 2-deoxy-2,3-dehydro-alpha-D-N-acetylneuraminic acid, competitive, 49% inhibition at 0.1 mM, 75% inhibition at 0.5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
neuraminic acid
-
-
NH4+
-
-
ononin
-
30% inhibition at 0.2 mM
oseltamivir
-
-
oseltamivir
-
molecular dynamics simulation study. In comparison with oseltamivir and zanamivir, peramivir shows strong direct ligand-enzyme hydrogen bonding, less space available in the N1 pocket, and it interacts tightly, via its OH group, with the D51 residue located in the 150-loop region
oseltamivir
-
; the robust neuraminidase activity of the 2009H1N1 virus is responsible for the high sensitivity of the virus to oseltamivir
oseltamivir
H7N9 influenza virus
R4JGE3, R4RZI6
;
oseltamivir carboxylate
-
-
oseltamivir carboxylate
-
-
oseltamivir carboxylate
-
-
oseltamivir carboxylate
A9YLL2, C3W5S3
;
oseltamivir phosphate
Q6DPL2
i.e. Tamiflu
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
50% inhibition at 0.065 mM
p-hydroxy oxamic acid
-
-
p-hydroxymercuribenzoate
-
; 0.001 mM, complete inhibition
p-hydroxyoxamic acid
-
competitive
p-nitrophenyloxamic acid
-
competitive
Palmatine
-
reversible non-competitive inhibition
peramivir
-
molecular dynamics simulation study
peramivir
-
-
peramivir
A9YLL2, C3W5S3
;
phaseollin
-
competitive inhibitor
plant extract
-
extracts of Laminaria saccharina, Fucus vesiculosus, Achillea millefolium, Allium cepa and Hypericum perforatum decrease activity. Extracts of pollen and garlic powder (Allium sativum) produce maximum inhibitory effect, 2fold inhibition after preoral administration
-
pseudocoptisine
-
-
-
pseudodehydrocorydaline
-
-
-
pterocarpin
-
noncompetitive
pyridoxal 5'-phosphate
-
65% inhibition at 1 mM
quercetin
-
-
reduced glutathione
-
-
rhamnocitrin
-
i.e. 3,4',5-trihydroxy-7-methoxyflavone
S-(2,4-diaminobutyl)-dihydrogen phosphorothioate
-
identified by ensemble-based virtual screening
scutellarein
-
-
SDS
Q8AAK9
0.9% residual activity at 5 mM
sialic acid
P06820
-
sialyl-2en
-
inhibits competitively the hydrolysis of Neu5Gcalpha-(2-5)-O-glycolylNeu5Gc
siastatin B
-
-
-
sophorapterocarpan A
-
noncompetitive inhibition
sulfuretin
-
i.e. 2-(Z-3',4'-dihydroxyphenylidene)-6-hydroxy-2,3-dihydrobenzofuran-3-one
tetrahydrocoptisine
-
-
-
thioglycolic acid
-
-
zanamivir
-
molecular dynamics simulation study
zanamivir
-
-
zanamivir
A9YLL2, C3W5S3
;
zanamivir
-
inhibition occurs in the millimolar range
zanamivir
-
inhibition occurs in the nanomolar range
zanamivir
-
inhibition occurs in the millimolar range
Zn2+
-
-
Zn2+
-
slight inhibition
Zn2+
-
61% residual activity at 10 mM
Zn2+
Q8AAK9
31.9% residual activity at 5 mM
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-benzene
-
15% inhibition at 1 mM
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-methanolO-(R/S)-epoxypropyl ether
-
27% inhibition at 1 mM
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-phenylmethane
-
37% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-2-phenylethane
-
14% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-benzene
-
14% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-methanol
-
28% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-methanol O-(R/S)-epoxypropyl ether
-
25% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-N-phthalimidylmethane
-
11% inhibition at 1 mM
[1-(methyl 6-deoxy-beta-D-galactopyranos-yl)-1H-1,2,3-triazol-4-yl]-phenylmethane
-
29% inhibition at 1 mM
Mn2+
-
28% inhibition at 10 mM
additional information
-
no inhibition by 5-amino sialic acid and sialic acid methyl ester
-
additional information
-
no inhibition by EDTA
-
additional information
-
the motility of the organism is decreased in vivo in presence of 2,3-didehydroneuraminic acid and sialic acid containing compounds
-
additional information
-
no inhibition by asteropine A
-
additional information
-
construction of homologous and heterologous dimers and trimers and trimers of inhibitors such as oseltamivir(R)-CH2-(R)oseltamivir, oseltamivir(R)-O-zanamivir, oseltamivir(R)-CH2-2-deoxy-2,3-dehydro-N-acetylneuraminic acid and calculation of molecular properties and ligand efficiency values
-
additional information
-
no inhibition by 4-acetamido-5-cyclopentanecarboxamido-2-fluoro benzoic acid of isoform NEU1 at 1 mM; no inhibition by 4-acetamido-5-cyclopentanecarboxamido-2-fluoro benzoic acid of NEU2 isoform at 1 mM; no inhibition by 4-acetamido-5-cyclopentanecarboxamido-2-fluoro benzoic acid of NEU3 isoform at 1 mM; no inhibition by 4-acetamido-5-cyclopentanecarboxamido-2-fluoro benzoic acid of NEU4 isoform at 1 mM
-
additional information
-
not inhibited by 4-[1-(methyl 6-deoxy-beta-D-galactopyranos-6-yl)-1H-1,2,3-triazol-4-yl]-butanoic acid and 4-[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazol-4-yl]-bromobenzene
-
additional information
-
design Influenza NA inhibitors that exhibit increased activity based on thiazolidine ring, evaluation of thiazolidine-4-carboxylic acid derivatives as Influenza neuraminidase inhibitors
-
additional information
-
inhibitory activity of triazole-containing carbocycles related to oseltamivir against virus-like particles containing the influenza virus neuraminidase-1 activity, overview
-
additional information
-
design of a sialidase inhibitor selective against human NEU2, inhibitor docking using also the crystal structure of the enzyme, overview
-
additional information
-
construction of octyl galactosides and octyl N-acetyl-lactosamines as trans-sialidase inhibitors, inhibitor screening of the beta-thiogalactopyranoside library, overview
-
additional information
-
not inhibited by EDTA
-
additional information
Q8AAK9
EDTA does not cause a significant decrease in activity
-
additional information
-
a 95% (v/v) ethanol extract of Corydalis turtschaninovii rhizome shows neuraminidase inhibitory activity (68% at 0.03 mg/ml); not inhibited by corydaline and tetrahydrocoptisine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acetic acid
-
-
Bovine serum albumin
-
-
-
cathepsin A
O35657
-
-
CMP
-
cytidine 5'-monophosphate is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator
dithiothreitol
-
-
dithiothreitol
-
1 mM, activates 2fold; activates
galactose
-
activates
glucomacropeptide
-
stimulates neuraminidase synthesis and activity about 2fold after 24 h
-
Lactic acid
-
-
N-acetylglucosamine
-
stimulates neuraminidase synthesis and activity about 8fold after 24 h
N-acetylmannosamine
-
stimulates neuraminidase synthesis and activity about 8fold after 24 h
octyl glucoside
-
highest activation (1.3-2.2fold) at 0.08% w/v concentration
Propionic acid
-
-
protective protein/cathepsin A
-
because of its association with protective protein/cathepsin A, which acts as a molecular chaperone, NEU1 is transported to the lysosomal compartment, catalytically activated, and stabilized
-
sialic acid
-
stimulates neuraminidase synthesis and activity about 25fold after 24 h
Sodium deoxycholate
-
highest activation (1.1-1.8.5fold) at 0.03% w/v concentration
Triton X-100
-
-
Triton X-100
-
-
Triton X-100
-
highest activation (1.5-2.5fold) at 0.02% w/v concentration
Trypsin
-
isoform NanI activity is enhanced by trypsin pretreatment and then only for substrates with an alpha-2,3- or alpha-2,6-sialic acid linkage
-
lactose
-
activates
additional information
-
isozymes Neu-1 and Neu-3 are induced in T-cells by CD3/CD28 co-stimulation, Neu-3 is the major inducible form in stimulated T-cells, specificity of induction, overview
-
additional information
-
the enzyme is activated at 37C and acidic pH, activation is dependent on time, pH and temperature, activation is inhibited by amastatin
-
additional information
-
the enzyme is activated at 37C and acidic pH, activation is dependent on time, pH and temperature, activation is not inhibited by amastatin
-
additional information
-
not affected by glucose, sucrose, and mannose, the motility of the organism is increased by about 6fold in vivo in presence of fetuin, alpha(2-3)-sialyllactose, and 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
additional information
-
bovine serum albumin does not increase enzyme activity
-
additional information
-
there is a negligible inducing effect at the 24th h for fetuin, and at the 48th h for transferrin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.06
(trifluoromethyl)umbelliferyl alpha-sialoside
-
wild-type, hydrolysis, pH 7.6, 25C
0.078
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid
-
in 50 mM Tris-HCl pH7.5, at 25C
0.028
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
pH 4.6, 37C, native wild-type enzyme
0.117
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
pH 4.6, 37C, recombinant mutant
1.4
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
-
1.1
2-(3-methoxyphenyl)-N-acetylneuraminic acid
-
-
2
2-(3-methoxyphenyl)-N-acetylneuraminic acid
-
-
0.0063
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
wild-type
0.0075
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
mutant Y252H
0.018
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
recombinant head domain, pH 6.5, 37C; wild-type, pH 6.5, 37C
0.027
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
mutant H274Y
0.053
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
mutant N294S
0.15
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
-
0.16
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
-
pH 5.2
0.6
2-O-(3-methoxyphenyl)neuraminic acid
-
-
0.018
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant H277F, pH 5.0, 37C
0.021
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant A160G, pH 5.0, 37C
0.032
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant R48S, pH 5.0, 37C
0.033
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant R48N, pH 5.0, 37C
0.038
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant E51S, pH 5.0, 37C
0.045
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant wild-type enzyme, pH 5.0, 37C
0.14
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant M87G, pH 5.0, 37C
0.16
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
3.3
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
pH 3.5, 37C
0.44
4-methylumbelliferyl alpha-NeuAc
-
37C, pH 5.6 or 7.0
7.9
4-methylumbelliferyl alpha-sialoside
C6JSW6
at 37C
3.1
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside
-
pH 3.5, 37C
-
0.04
4-methylumbelliferyl-3-deoxy-D-glycero-D-galactononulosonic acid
-
-
-
0.00017
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
pH 6.5, 37C
0.0187
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q37QH2
in 70 mM sodium citrate, at pH 7.0 and 37C
0.059
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
pH 5.5, 37C
0.07
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.08
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
DSM 756, large salidase and A99, large salidase
0.09
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.09
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.1
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.11
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.14
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.17
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
K1
0.18
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
A99, small salidase, cloned
0.19
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
A99, small salidase
0.19
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
wild type
0.2
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
glomeruli
0.21
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
K2
0.23
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
cortex
0.25
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.5
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, wild-type enzyme
0.67
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
1.1
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, mutant S418A
1.8
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
K3
3
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
4.9
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, mutant Y262F
0.11
4-nitrophenyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
53
4-nitrophenyl alpha-D-N-acetylneuraminic acid
-
-
0.03
4-nitrophenyl alpha-sialoside
-
mutant D59A, hydrolysis, pH 7.6, 25C
1.09
4-nitrophenyl alpha-sialoside
-
wild-type, hydrolysis, pH 7.6, 25C
7.3
4-nitrophenyl alpha-sialoside
C6JSW6
at 37C
4.1
5-N-acetyl-2-O-(3-methoxyphenyl)-alpha-D-neuraminic acid
-
-
0.063
alpha(2-3')-sialyllactose
Q9EZV6, Q9EZV7
recombinant enzyme, pH 6.8, 37C
0.0049
alpha(2-3)-sialyllactose
-
pH 6.5, 37C
0.144
alpha(2-3)-sialyllactose
-
pH 5.5, 37C
5.5
alpha(2-3)-sialyllactose
C6JSW6
at 37C
0.31
alpha(2-3)sialyllactose
-
37C, pH 5.6 or 7.0
0.28
alpha(2-3)sialylparagloboside
-
37C, pH 5.6 or 7.0
0.43
alpha(2-3)sialylparagloboside
-
37C, pH 5.6 or 7.0
7
alpha(2-6)-sialyllactose
C6JSW6
at 37C
0.18
alpha-(2-3)-sialyllactose
-
pH 5.2
0.52
alpha-(2-6)-sialyllactose
-
pH 5.2
0.15
alpha-phenyl-N-acetylneuraminoside
-
-
-
0.5
alpha-sialyllactose
-
-
0.6 - 0.8
alpha-sialyllactose
-
strain dependent
0.86
alpha-sialyllactose
-
-
1
alpha-sialyllactose
-
-
1.2 - 1.6
alpha-sialyllactose
-
-
2.1
alpha-sialyllactose
-
-
3.3
alpha-sialyllactose
-
-
0.01
alpha1-Acid glycoprotein
-
-
-
0.36
bovine submaxillary mucin
-
-
-
0.29
colominic acid
Q8AAK9
pH 7.0 and 37C
1.47
colominic acid
-
-
0.0029
fetuin
-
pH 5.5, 37C
-
0.008
fetuin
-
-
-
0.1
fetuin
-
-
-
0.1
fetuin
-
-
-
0.36
fetuin
-
-
-
0.45
fetuin
-
37C, pH 6.7; pH 6.5, 37C
-
2.1
fetuin
-
-
-
2.53
fetuin
-
-
-
6.7
fetuin
-
-
-
0.27
Galp(beta1,2)Galf(beta1,4)GlcNAc
-
-
0.31
Galp(beta1,2)Galf(beta1,4)GlcNAcOBn
-
-
0.09
Galp(beta1,2)Galf(beta1,4)GlcNAcol
-
-
0.08
Galp(beta1,2)[Galp(beta1,3)]Gal
-
-
0.17
Galp(beta1,2)[Galp(beta1,3)]GalOBn
-
-
0.25
Galp(beta1,2)[Galp(beta1,3)]Galol
-
-
0.17
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
0.1
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
0.82
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
2.35
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
1.07
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
0.18
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
0.17
Galp(beta1,6)GlcNAc
-
-
0.05
Galp(beta1,6)GlcNAcOBn
-
-
0.08
Galp(beta1,6)GlcNAcol
-
-
0.15
Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
0.08
Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
0.14
ganglioside GD1a
-
37C, pH 5.6 or 7.0 activated by 0.02% Triton X-100
0.91
ganglioside GD1a
-
37C, pH 5.6 or 7.0, without Triton X-100
0.51
ganglioside GD1b
-
37C, pH 5.6 or 7.0 activated by 0.02% Triton X-100
0.59
ganglioside GD1b
-
37C, pH 5.6 or 7.0, without Triton X-100
0.24
ganglioside GM3
-
37C, pH 5.6 or 7.0, activated by 0.02% Triton X-100
0.4
ganglioside GM3
-
37C, pH 5.6 or 7.0, without Triton X-100
0.38
ganglioside GT1b
-
37C, pH 5.6 or 7.0 activated by 0.02% Triton X-100
0.62
ganglioside GT1b
-
37C, pH 5.6 or 7.0, without Triton X-100
0.02
GD1a
-
-
0.11
GD1a
-
-
0.25
GD1a
-
-
1.75
GD1a
-
-
0.1
GD1b
-
-
0.13
GD1b
-
-
0.083
glucomacropeptide
-
37C, sialidase isoenzyme NA2
-
0.126
glucomacropeptide
-
37C, sialidase isoenzyme NA1
-
0.04
GM2
-
-
0.15
GM3
-
-
0.01
GQ1
-
-
0.09
GT1
-
-
1.6
N-acetylneuraminic acid-alpha-2,3-lactose
-
-
2.2
N-acetylneuraminic acid-alpha-2,3-lactose
-
-
7.9
N-acetylneuraminic acid-alpha-2,3-lactose
-
-
0.8
N-acetylneuraminic acid-alpha-2,6-galactose
-
-
1.2
N-acetylneuraminic acid-alpha-2,6-lactose
-
-
2
N-acetylneuraminic acid-alpha-2,6-lactose
-
-
5.6
N-acetylneuraminic acid-alpha-2,6-lactose
-
-
0.78
N-Acetylneuraminyllactose
-
-
0.9
N-Acetylneuraminyllactose
-
-
1.8
N-Acetylneuraminyllactose
-
-
3
N-Acetylneuraminyllactose
-
-
4.8
N-glycoloylneuraminic acid-alpha-2,3-lactose
-
-
-
0.17
Neu5Ac(2-3)-alpha-lactose
-
37C, pH 6.7
0.17
Neu5Ac(alpha2,3)-lactose
-
pH 6.5, 37C
0.261
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium
-
pH 9.5, 37C
0.385
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium
-
pH 6.0, 37C
0.138
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium
-
pH 9.5, 37C
0.14
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium
-
pH 6.0, 37C
0.45
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium
-
pH 9.5, 37C
0.021
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium
-
pH 6.0, 37C
0.216
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium
-
pH 9.5, 37C
1.74
orosomucoid
-
-
-
0.000045
phenyl alpha-sialoside
-
pH 5.25, 37C, mutant enzyme Y370G
0.000023
phenyl beta-sialoside
-
pH 5.25, 37C, mutant enzyme Y370G
0.36
poly-alpha-2,8-lactose
-
A99, large salidase
-
0.5
poly-alpha-2,8-lactose
-
DSM 756, large salidase
-
0.1
sialyl-alpha-2,3-lactose
-
-
0.53
sialyl-alpha-2,3-lactose
-
A99, small salidase
0.56
sialyl-alpha-2,3-lactose
-
A99, large salidase
0.58
sialyl-alpha-2,3-lactose
-
DSM 756, large salidase
1.47
sialyl-alpha-2,3-lactose
Q8AAK9
pH 7.0 and 37C
1.67
sialyl-alpha-2,3-lactose
-
-
1.67
sialyl-alpha-2,3-lactose
-
-
1.9
sialyl-alpha-2,3-lactose
-
A99, small salidase, cloned
4.9
sialyl-alpha-2,3-lactose
-
-
0.13
sialyl-alpha-2,6-lactose
-
-
0.49
sialyl-alpha-2,6-lactose
Q8AAK9
pH 7.0 and 37C
1.7
sialyl-alpha-2,6-lactose
-
A99, large salidase
1.81
sialyl-alpha-2,6-lactose
-
-
1.91
sialyl-alpha-2,6-lactose
-
-
2.2
sialyl-alpha-2,6-lactose
-
DSM 756, large salidase
2.8
sialyl-alpha-2,6-lactose
-
A99, small salidase, cloned
3.3
sialyl-alpha-2,6-lactose
-
-
3.5
sialyl-alpha-2,6-lactose
-
A99, small salidase
0.52
sialyllactose
-
-
0.68
sialyllactose
-
-
0.8
sialyllactose
-
-
0.92
sialyllactose
-
intralysosomal sialidase
2.38
sialyllactose
-
cytosolic sialidase
1.79
Transferrin
-
-
-
2.39
Transferrin
-
-
-
0.03
GT1b
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
Q9Q2W5
kinetics and thermodynamics of wild-type and mutant enzymes
-
additional information
additional information
-
analysis of Michaelis enzyme-substrate complexes
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.9
(trifluoromethyl)umbelliferyl alpha-sialoside
-
wild-type, hydrolysis, pH 7.6, 25C
0.0743
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
pH 3.5, 37C
8
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant M87G, pH 5.0, 37C
23
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant wild-type enzyme, pH 5.0, 37C
27
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutants A160G and H277F, pH 5.0, 37C
32
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant R48N, pH 5.0, 37C
44
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant R48S, pH 5.0, 37C
51
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
recombinant mutant E51S, pH 5.0, 37C
146.7
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
0.0004
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside
-
pH 3.5, 37C
-
45
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
-
-
0.88
4-nitrophenyl alpha-D-N-acetylneuraminic acid
-
-
82.4
4-nitrophenyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
0.031
4-nitrophenyl alpha-sialoside
-
mutant D59A, hydrolysis, pH 7.6, 25C
1.57
4-nitrophenyl alpha-sialoside
-
wild-type, hydrolysis, pH 7.6, 25C
0.82
phenyl alpha-sialoside
-
pH 5.25, 37C, mutant enzyme Y370G
13.3
phenyl beta-sialoside
-
pH 5.25, 37C, mutant enzyme Y370G
313.5
sialyl-alpha-2,3-lactose
Q8AAK9
pH 7.0 and 37C
161
sialyl-alpha-2,6-lactose
Q8AAK9
pH 7.0 and 37C
157.6
colominic acid
Q8AAK9
pH 7.0 and 37C
additional information
additional information
-
kcat at pH 9.5, 37C, relative to the reported kcat at pH 6.0, 37C, with substrate 4-nitrophenyl alpha-D-N-acetylneurminide
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
330
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid
Q4WQS0
mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37C
0
11600
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid
Q4WQS0
mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37C
0
26800
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid
Q4WQS0
mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37C
0
47700
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid
Q4WQS0
wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37C
0
916.9
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
4440
14500
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q4WQS0
mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37C
4440
19000
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q4WQS0
mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37C
4440
22300
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
-
pH 3.5, 37C
4440
53500
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q4WQS0
wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37C
4440
74200
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
Q4WQS0
mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37C
4440
144
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside
-
pH 3.5, 37C
0
729.2
4-nitrophenyl alpha-D-N-acetylneuraminic acid
Q8AAK9
pH 7.0 and 37C
40100
8
alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside
Q4WQS0
wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37C
0
200
alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside
Q4WQS0
mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37C
0
1620
alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside
Q4WQS0
mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37C
0
8900
alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside
Q4WQS0
mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37C
0
541.2
colominic acid
Q8AAK9
pH 7.0 and 37C
4077
213.3
sialyl-alpha-2,3-lactose
Q8AAK9
pH 7.0 and 37C
7426
328.6
sialyl-alpha-2,6-lactose
Q8AAK9
pH 7.0 and 37C
7427
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.06
(1R/S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-(4-phenethyl-[1,2,3]triazol-1-yl)cyclohex-2-ene-1-carboxylic acid
-
pH 7.4, 37C
0.019
(1R/S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxy-1-methylethyl)[1,2,3]triazol-1-yl]cyclohex-2-ene-1-carboxylic acid
-
pH 7.4, 37C
0.0000048
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-ethoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.000021
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-ethoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000006
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000198
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.00000067
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000173
(2R,4S,5R)-5-[(1R,2R)-1-(acetylamino)-2-methoxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000012
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-hydroxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000013
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-hydroxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.00000014
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-methoxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.00000021
(2R,4S,5R)-5-[(1R,2S)-1-(acetylamino)-2-methoxy-2-methylpentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.000002
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.000025
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybut-3-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000028
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybutyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000115
(2R,4S,5R)-5-[(1S,2S)-1-(acetylamino)-2-hydroxybutyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000016
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.00001
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypent-4-en-1-yl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000013
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000053
(2R,4S,5R)-5-[(2S)-1-(acetylamino)-2-hydroxypentyl]-4-[(1Z)-prop-1-en-1-yl]pyrrolidine-2-carboxylic acid
-
-
0.0000015
(3S,4R,5R)-4-acetamido-3-amino-5-(1-ethylpropoxy)cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.0058
(3S,4R,5R)-4-acetamido-3-[4-((17alpha)-estra-1,3,5(10)-triene-3,17-dihydroxy-17-yl)[1,2,3]triazol-1-yl]-5-(1-ethylpropoxy)cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.000072
(3S,4R,5R)-4-acetamido-5-(1-ethyl-propoxy)-3-[4-(1-hydroxypropyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.0012
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-(4-phenethyl-[1,2,3]triazol-1-yl)cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.00000046
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-guanidinocyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.00013
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxy-1-methylethyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.0048
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxycyclohexyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.011
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(1-hydroxycyclopentyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.00046
(3S,4R,5R)-4-acetamido-5-(1-ethylpropoxy)-3-[4-(3-hydroxypropyl)[1,2,3]triazol-1-yl]cyclohex-1-ene-1-carboxylic acid
-
pH 7.4, 37C
0.0021
(6aS,12aS)-6a,12a-dihydro-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-3-ol
-
-
0.06
(6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid
-
pH 6.5, 37C
0.000058
1,3,6,7-tetrahydroxy-2-(3-methylbut-2-enyl)-8-(2-methylbut-3-en-2-yl)-9H-xanthen-9-one
Q59310
pH 5.0, 37C
0.000187
11,17-dihydroxy-3,20-dioxopregn-4-en-21-yl cyclopentanecarboxylate
-
predicted Ki value
0.04
2,3 didehydroneuraminic acid
-
-
0.028
2,3-didehydro-2-deoxy-N-acetylneuraminic acid
-
pH 4.6, 37C, native wild-type enzyme
0.117
2,3-didehydro-2-deoxy-N-acetylneuraminic acid
-
pH 4.6, 37C, recombinant mutant
0.03
2,3-didehydroneuraminic acid
-
pH 5.5, 37C
0.000026
2,4-diaminohexahydropyrimidine-5-carbonitrile
-
predicted Ki value
0.00018
2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-3-(4-hydroxy-3-nitrophenyl)propanoic acid
-
predicted Ki value
0.06
2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid
-
-
0.035
2-deoxy-2,3-dehydro-N-acetyl neuraminic acid
Q8AAK9
pH 7.0 and 37C
0.0053
2-Deoxy-2,3-didehydro-N-acetylneuraminic acid
-
pH 6.5, 37C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
0.00004
3-hydroxy-4-[(E)-naphthalen-1-yldiazenyl]naphthalene-2,7-disulfonic acid
-
predicted Ki value
16.2
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, mutant S418A
24.7
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, wild-type enzyme
28.1
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
Q9Q2W5
pH 7.5, mutant Y262F
0.185
4-nitrophenyl oxamic acid
-
pH 5.5, 37C
1.9
5-acetamido-2-(4-N-5-dimethylaminonaphthalene-1-sulfonyl-2-difluoromethylphenyl)3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosonic acid
-
-
0.000016
6,6-dimethyl-1-(3-[(3-nitrobenzyl)oxy]phenyl)-1,3,5-triazinane-2,4-diimine
-
predicted Ki value
0.00089
6-chloro-9,10-dihydro-4,5,7-trihydroxy-9,10-dioxo-2-anthracenecarboxylate
-
in 50 mMTris-HCl pH7.5, at 25C
0.0000367
asteropine A
-
-
-
0.00034
asteropine A
-
-
-
0.00209
calopocarpin
-
-
0.00031
cristacarpin
-
-
0.000136
cudratricusxanthone
Q59310
pH 5.0, 37C
0.000138
cudraxanthone L
Q59310
pH 5.0, 37C
0.000098
cudraxanthone M
Q59310
pH 5.0, 37C
0.00383
demethylmedicarpin
-
-
0.0018
erystagallin A
-
-
0.0152
erysubin D
-
-
0.00024
erysubin E
-
-
0.00413
erythribyssin L
-
-
0.00121
erythribyssin O
-
-
0.00255
eryvarin D
-
-
0.01096
isoneorautenol
-
-
0.000103
macluraxanthone
Q59310
pH 5.0, 37C
0.042
N-bromosuccinimide
-
pH 6.5, 37C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
0.053
N-ethylmaleimide
-
pH 6.5, 37C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
0.02174
neorautenol
-
-
0.00000032
oseltamivir
-
wild-type
0.000063
oseltamivir
-
predicted Ki value
0.0000001
oseltamivir carboxylate
-
-
0.0000011
oseltamivir carboxylate
-
-
0.0000013
oseltamivir carboxylate
-
wild-type, pH 6.5, 37C
0.0000024
oseltamivir carboxylate
-
recombinant head domain, pH 6.5, 37C
0.04
p-chloromercuribenzoate
-
pH 6.5, 37C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
0.15
p-hydroxy oxamic acid
-
-
0.15
p-hydroxyoxamic acid
-
pH 6.5, 37C
0.03
p-nitrophenyl oxamic acid
-
-
0.00484
phaseollin
-
-
0.0017
pterocarpin
-
-
0.0002
S-(2,4-diaminobutyl)-dihydrogen phosphorothioate
-
predicted Ki value
0.00178
sophorapterocarpan A
-
-
0.00000006
zanamivir
-
-
0.0000001
zanamivir
-
-
0.0000001
zanamivir
-
wild-type
0.000133
zanamivir
-
predicted Ki value
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.058
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(acetylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
0.013
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(benzoylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
0.032
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-(butyrylamino)-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
0.68
(2R,3R,4S)-3-(acetylamino)-2-[(1S,2S)-3-[(cyclopropylcarbonyl)amino]-1,2-dihydroxypropyl]-4-hydroxy-3,4-dihydro-2H-pyran-6-carboxylic acid
-
-
0.12
(3E)-3-(1,3-benzothiazol-2-yl)-4-(2,5-dimethylphenyl)but-3-enoate
-
-
0.16
(3E)-3-(1,3-benzothiazol-2-yl)-4-(2-ethoxyphenyl)but-3-enoate
-
-
0.18
(3E)-3-(1,3-benzothiazol-2-yl)-4-(3,4-dimethoxyphenyl)but-3-enoate
-
-
0.12
(3E)-3-(1,3-benzothiazol-2-yl)-4-(3-fluorophenyl)but-3-enoate
-
-
0.15
(3E)-3-(1,3-benzothiazol-2-yl)-4-[2-(propan-2-yloxy)phenyl]but-3-enoate
-
-
0.15
(3E)-3-(1,3-benzothiazol-2-yl)-4-[2-methyl-5-(trifluoromethyl)phenyl]but-3-enoate
-
-
0.0181
(4R)-2-(2-carboxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication
0.0107
(4R)-2-(2-carboxyphenyl)-3-(chloroacetyl)-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication
0.00192
(4R)-2-(2-carboxyphenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication
0.00028
(4R)-2-(2-carboxyphenyl)-3-glycyl-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication
0.0123
(4R)-2-(2-hydroxy-3-methoxyphenyl)-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication
0.00162
(4R)-2-(2-hydroxy-3-methoxyphenyl)-3-(phenylacetyl)-1,3-thiazolidine-4-carboxylic acid
-
pH and temperature not specified in the publication