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Information on EC 3.2.1.130 - glycoprotein endo-alpha-1,2-mannosidase and Organism(s) Homo sapiens

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IUBMB Comments
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al .
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This record set is specific for:
Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
endomannosidase, manea, endo-alpha-d-mannosidase, endo-alpha-mannosidase, golgi-endomannosidase, alpha-endomannosidase, glucosyl mannosidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-1,2-endomannosidase
-
alpha-endomannosidase
-
-
endo-alpha-D-mannosidase
-
-
-
-
endo-alpha-mannosidase
endomannosidase
-
-
-
-
glucosyl mannosidase
-
-
-
-
glucosylmannosidase
-
-
-
-
Golgi endo-a-mannosidase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
glycoprotein glucosylmannohydrolase
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al [7].
CAS REGISTRY NUMBER
COMMENTARY hide
108022-16-8
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125858-79-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-N-dansylaminopropyl [alpha-D-glucopyranosyl-(1->3)]-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl (1->2)-alpha-D-mannopyranoside + H2O
3-N-dansylaminopropyl-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + ?
show the reaction diagram
-
-
-
-
?
GlcMan5GlcNAc2 + H2O
alpha-D-glucosyl-1,3-D-mannopyranose + Man4GlcNAc2
show the reaction diagram
37°C
-
-
?
GlcMan9GlcNAc + H2O
Man8GlcNAc + glucosyl alpha1,3-mannose
show the reaction diagram
-
-
-
-
?
GlcMan9GlcNAc2-[protein] + H2O
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
show the reaction diagram
the enzyme removes Glc-alpha-(1->3)-Man unit from G1M9-native and -misfolded-crambins. The enzyme does not have specific preference for correctly folded or misfolded protein structure
-
-
?
additional information
?
-
the enzyme is able to mediate transglycosylation from alpah-glycosyl-fluorides
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
optimum for transglycosylation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
most prominent
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the alpha-endomannosidase gene is associated with panic disorder and social anxiety disorder
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MANEA_HUMAN
462
1
53671
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
x * 55000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E407D
the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)-Man
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expresion in Escherichia coli
expression in Escherichia coli
expression in Pichia pastoris
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the level of alpha-endomannosidase mRNA is reduced to 89%, 54% and 34% at 2, 5 and 10 h, respectively, after the addition of actinomycin D
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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cocaine-induced paranoia is associated with 6 single-nucleotide polymorphisms in European American families and 9 single-nucleotide polymorphisms in African American families. Association of MANEA single-nucleotide polymorphisms with cocaine dependence in both family samples is much weaker. The A allele of the 3' untranslated region single-nucleotide polymorphism rs9387522 is associated with increased risk of cocaine-induced paranoia
synthesis
the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)Man. The synthetic utility of the enzyme is demonstrated by generation of a high-mannose-type undecasaccharide (Glc1Man9GlcNAc2)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hamilton, S.R.; Li, H.; Wischnewski, H.; Prasad, A.; Kerley-Hamilton, J.S.; Mitchell, T.; Walling, A.J.; Davidson, R.C.; Wildt, S.; Gerngross, T.U.
Intact alpha-1,2-endomannosidase is a typical type II membrane protein
Glycobiology
15
615-624
2005
Homo sapiens (Q5SRI9), Homo sapiens, Rattus norvegicus (Q5GF25)
Manually annotated by BRENDA team
Farrer, L.A.; Kranzler, H.R.; Yu, Y.; Weiss, R.D.; Brady, K.T.; Anton, R.; Cubells, J.F.; Gelernter, J.
Association of variants in MANEA with cocaine-related behaviors
Arch. Gen. Psychiatry
66
267-274
2009
Homo sapiens
Manually annotated by BRENDA team
Iwamoto, S.; Kasahara, Y.; Kamei, K.; Seko, A.; Takeda, Y.; Ito, Y.; Matsuo, I.
Measurement of endo-alpha-mannosidase activity using a fluorescently labeled oligosaccharide derivative
Biosci. Biotechnol. Biochem.
78
927-936
2014
Homo sapiens
Manually annotated by BRENDA team
Jensen, K.P.; Stein, M.B.; Kranzler, H.R.; Yang, B.Z.; Farrer, L.A.; Gelernter, J.
The alpha-endomannosidase gene (MANEA) is associated with panic disorder and social anxiety disorder
Transl. Psychiatry
4
e353
2014
Homo sapiens
Manually annotated by BRENDA team
Dedola, S.; Izumi, M.; Makimura, Y.; Seko, A.; Kanamori, A.; Takeda, Y.; Ito, Y.; Kajihara, Y.
Direct assay for endo-alpha-mannosidase substrate preference on correctly folded and misfolded model glycoproteins
Carbohydr. Res.
434
94-98
2016
Homo sapiens (Q5SRI9)
Manually annotated by BRENDA team
Iwamoto, S.; Kasahara, Y.; Yoshimura, Y.; Seko, A.; Takeda, Y.; Ito, Y.; Totani, K.; Matsuo, I.
Endo-alpha-mannosidase-catalyzed transglycosylation
ChemBioChem
18
1376-1378
2017
Homo sapiens (Q5SRI9)
Manually annotated by BRENDA team