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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
endogalactosaminidase,
more
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(2-amino-2-deoxy-alpha-D-galactopyranosyl-(1-4))n-2-amino-2-deoxy-alpha-D-galactopyranose + H2O = (2-amino-2-deoxy-alpha-D-galactopyranosyl-(1-4))n-m-2-amino-2-deoxy-alpha-D-galactopyranose + (2-amino-2-deoxy-alpha-D-galactopyranosyl-(1-4))m-2-amino-2-deoxy-alpha-D-galactopyranose
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hydrolysis of O-glycosyl bond
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galactosaminoglycan glycanohydrolase
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oligogalactosaminoglycan + H2O
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endohydrolysis of 1,4-alpha-galactosaminidic linkages
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polygalactosaminoglycan + H2O
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high molecular weight substrate obtained from Neurospora
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polygalactosaminoglycan + H2O
galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN
additional information
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sporeling detaching activity, not: N-acetyl-oligogalactosaminoglycan and chitosan
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polygalactosaminoglycan + H2O
galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN
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alpha-1,4-linkage
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polygalactosaminoglycan + H2O
galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN
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alpha-1,4-linkage
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polygalactosaminoglycan + H2O
galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN
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high molecular weight substrate obtained from Neurospora
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polygalactosaminoglycan + H2O
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high molecular weight substrate obtained from Neurospora
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additional information
additional information
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0.05% polygalactosaminoglycan
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5.4
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sporeling-detaching activity
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3 - 8
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very low activity at pH 3 and 8
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881
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brenda
881
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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A0A364NHH5_9PLEO
473
0
52586
TrEMBL
other Location (Reliability: 2)
A0A5S9VY49_9FLAO
271
0
31646
TrEMBL
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A0A2H1E999_9FLAO
271
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31646
TrEMBL
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31000
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gel filtration, SDS-PAGE
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monomer
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1 * 31000, SDS-PAGE
monomer
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1 * 31000, SDS-PAGE
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2
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20°C, inactivated within a few min
135716
4 - 7
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citrate-phosphate buffer
135717
7
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quite stable at neutrality
135716
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20
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pH 2, inactivated within a few min
70
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15 min, 60% loss of activity
additional information
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heat stability increases at less acid pH
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repeated freezing and thawing, no loss of activity
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-20°C, 8 months, distilled water, stable to repeated freezing and thawing
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Reissig, J.L; Lai, H.H.; Glasgow, J.E.
An endogalactosaminidase from Streptomyces griseus
Can. J. Biochem.
53
1237-1249
1975
Streptomyces griseus
brenda
Tamura, J.i.; Takagi, H.; Kadowaki, K.
Purification and some properties of the endo alpha-1,4 polygalactosaminidase from Pseudomonas sp.
Agric. Biol. Chem.
52
2475-2484
1988
Pseudomonas sp., Pseudomonas sp. 881
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brenda
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