Information on EC 3.1.6.1 - arylsulfatase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.6.1
-
RECOMMENDED NAME
GeneOntology No.
arylsulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a phenol sulfate + H2O = a phenol + sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sphingolipid metabolism
-
-
Steroid hormone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-sulfate sulfohydrolase
A group of enzymes with rather similar specificities.
CAS REGISTRY NUMBER
COMMENTARY hide
9016-17-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain R-0827 and strain R-3523
-
-
Manually annotated by BRENDA team
strain R-3631 and strain R-1210
-
-
Manually annotated by BRENDA team
var. fermentarious
-
-
Manually annotated by BRENDA team
strain R-0638
-
-
Manually annotated by BRENDA team
strain R-0638
-
-
Manually annotated by BRENDA team
strain SH 10-1, strain SH 10-3, strain SH 21 and strain SH 10-6
-
-
Manually annotated by BRENDA team
strain R-1031
-
-
Manually annotated by BRENDA team
strain R-1031
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
marked derepression of enzyme in synthetic medium by addition of tyramine
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain W70
-
-
Manually annotated by BRENDA team
strain W70
-
-
Manually annotated by BRENDA team
BL-21 strain
-
-
Manually annotated by BRENDA team
BL-21 strain
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Littorina kurila
-
-
-
Manually annotated by BRENDA team
soil isolate
UniProt
Manually annotated by BRENDA team
soil isolate
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
soil isolate
UniProt
Manually annotated by BRENDA team
soil isolate
UniProt
Manually annotated by BRENDA team
IFO 3046
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain S980-14, arylsulfatase Es-1
-
-
Manually annotated by BRENDA team
strain S980-14, arylsulfatase Es-1
-
-
Manually annotated by BRENDA team
soil isolate
UniProt
Manually annotated by BRENDA team
strain T109-3, arylsulfatase Es-2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
2'',3''-di-O-acetyletoposide + sulfate
show the reaction diagram
2-carboxyphenyl sulfate + H2O
2-carboxyphenol + sulfate
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl sulfate + H2O
sulfate + 2-chloro-4-nitrophenol
show the reaction diagram
-
-
-
-
?
2-hydroxy-4-nitrophenyl sulfate + H2O
2-hydroxy-4-nitrophenol + sulfate
show the reaction diagram
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
show the reaction diagram
2-methyl-4-nitrophenyl sulfate + H2O
2-methyl-4-nitrophenol + sulfate
show the reaction diagram
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
show the reaction diagram
3-carboxyphenyl sulfate + H2O
3-carboxyphenol + sulfate
show the reaction diagram
3-indoxyl sulfate + H2O
1H-indol-3-ol + sulfate
show the reaction diagram
-
6.4% of the activity with 4-nitrophenyl sulfate
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
show the reaction diagram
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
show the reaction diagram
4-hydroxy-2-nitrophenyl sulfate + H2O
4-hydroxy-2-nitrophenol + sulfate
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-nitrophenyl sulfate + H2O
4-hydroxy-3-nitrophenol + sulfate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
show the reaction diagram
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
show the reaction diagram
4-methylumbelliferylsulfate + H2O
4-methylumbelliferol + sulfate
show the reaction diagram
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
show the reaction diagram
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
show the reaction diagram
6-benzoyl-2-naphthyl sulfate + H2O
6-benzoyl-2-naphthol + sulfate
show the reaction diagram
-
-
-
-
?
agar + H2O
?
show the reaction diagram
-
-
-
-
?
androsterone sulfate + H2O
androsterone + sulfate
show the reaction diagram
-
-
-
-
?
apigenin-7,4'-disulfate + H2O
sulfate + ?
show the reaction diagram
-
-
-
-
?
ascorbic acid 2-sulfate + H2O
ascorbic acid + sulfate
show the reaction diagram
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
show the reaction diagram
-
-
-
-
?
cerebroside 3-sulfate + H2O
?
show the reaction diagram
-
-
-
-
-
cerebroside 3-sulfate + H2O
cerebroside + sulfate
show the reaction diagram
-
-
-
-
?
chondroitin sulfate C + H2O
chondroitin + sulfate
show the reaction diagram
dopamine 3-sulfate + H2O
dopamine + sulfate
show the reaction diagram
-
-
-
-
-
dopamine 4-sulfate + H2O
dopamine + sulfate
show the reaction diagram
-
-
-
-
-
epinephrine 3-sulfate + H2O
epinephrine + sulfate
show the reaction diagram
-
-
-
-
-
epinephrine 4-sulfate + H2O
epinephrine + sulfate
show the reaction diagram
-
-
-
-
-
estrone 3-sulfate + H2O
sulfate + estrone
show the reaction diagram
etoposide 4'-sulfate + H2O
etoposide + sulfate
show the reaction diagram
hydroquinone sulfate + H2O
hydroquinone + sulfate
show the reaction diagram
-
-
-
-
?
indoxyl sulfate + H2O
indoxol + sulfate
show the reaction diagram
-
weak activity
-
-
?
methylumbelliferyl sulfate + H2O
methylumbelliferone + sulfate
show the reaction diagram
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
show the reaction diagram
-
-
-
-
?
nordopamine 3-sulfate + H2O
nordopamine + sulfate
show the reaction diagram
-
-
-
-
-
norepinephrine 3-sulfate + H2O
norepinephrine + sulfate
show the reaction diagram
-
-
-
-
-
norepinephrine 4-sulfate + H2O
norepinephrine + sulfate
show the reaction diagram
-
-
-
-
-
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
show the reaction diagram
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
show the reaction diagram
phenyl sulfate + H2O
phenol + sulfate
show the reaction diagram
phloroglucinol monosulfate + H2O
phloroglucinol + sulfate
show the reaction diagram
-
-
-
-
?
Tyr-O-sulfate + H2O
Tyr + sulfate
show the reaction diagram
-
weak activity
-
-
?
tyramine-O-sulfate + H2O
tyramine + sulfate
show the reaction diagram
-
weak activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
agar + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
can partially replace Ca2+
Mg2+
-
can partially replace Ca2+
NH4+
-
25 mM, 60% activation
phosphate
-
activates hydrolysis of 4-nitrophenyl sulfate with the beta enzyme
Sr2+
-
can partially replace Ca2+
sulfate
-
activates hydrolysis of 4-nitrophenyl sulfate with the beta enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
1,10-phenanthroline
-
-
1,2,3-benzoxathiazine 2,2-dioxide
-
irreversible
1,3-phenanthroline
-
-
2-(difluoromethyl)phenyl hydrogen sulfate
-
competitive
2-hydroxy-4-methyl-5-nitrophenyl sulfate
-
substrate inhibition
2-mercaptoethanol
-
-
3,4-dihydro-1,2,3-benzoxathiazine 2,2-dioxide
-
irreversible
3-(alpha-acetonylbenzyl)-4-hydroxycoumarin
-
2.5 mM results in 50% reduced activity of arylsulfatase
3H-1,2,3-benzoxathiazole 2,2-dioxide
-
irreversible
4-(difluoromethyl)phenyl hydrogen sulfate
-
competitive
4-nitrophenyl phosphate
4-nitrophenyl sulfate
5-sulfosalicylic acid
-
strong
AgNO3
-
0.2 mM, 20-30% inhibition
Borate
citrate
dithiothreitol
dopamine
Estrone 3-sulfate
-
-
heparin
-
-
hydroxylamine
imidazole
-
-
iodoacetate
iodoacetic acid
-
10 mM, 48% residual activity
IPTG
-
at concentrations from 50 micromolar up to 5000 micromolar of IPTG the activity decreases by more than 50%
K2HPO4
KCN
-
10 mM, 23% residual activity
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester
-
hydrolysis of 4-nitrophenyl sulfate
Na2B4O7
-
10 mM, 12% residual activity
Na2SO3
Na3PO4
octopamine
p-(amidinophenyl)methanesulfonyl fluoride
-
-
p-(amidinophenyl)methanesulfonyl fluoride HCl
-
-
Phenolphthalein disulfate
-
hydrolysis of 4-nitrophenyl sulfate
phenyl sulfamate
-
irreversible
phosphate
praziquantel
-
schistosomical drug, inhibitory to enzyme. No effect on immunological properties of enzyme
sodium dodecyl sulfonate
-
-
sodium dodecylsulfate
-
1 mM, 4% residual activity
sodium phenylmethane sulfonate
-
-
tyramine
warfarin
-
-
ZnCl2
-
10 mM, 10% residual activity
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Commiphora extract
-
schistosomical drug, activating to enzyme. No effect on immunological properties of enzyme
-
EDTA
-
10 mM, 114% of initial activity
imidazole
-
strongly increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.181
2-hydroxy-4-nitrophenyl sulfate
-
in presence of 100 mM acetate
0.043 - 0.085
2-hydroxy-5-nitrophenyl sulfate
0.55
2-methyl-4-nitrophenyl sulfate
-
pH 8.9, 30C
0.123
4-hydroxy-2-nitrophenyl sulfate
-
in presence of 100 mM acetate
0.193
4-hydroxy-3-nitrophenyl sulfate
-
in presence of 100 mM acetate
2.5
4-methylumbelliferone sulfate
-
-
0.0066
4-Methylumbelliferyl sulfate
-
pH 8.9, 30C
0.105 - 20.6
4-nitrocatechol sulfate
0.0291 - 0.0752
4-nitrophenyl phosphate
0.00025 - 26
4-nitrophenyl sulfate
0.5
Ascorbate 2-sulfate
-
-
2.5
Ascorbic acid 2-sulfate
-
-
0.13
cerebroside 3-sulfate
-
-
7.2 - 9.8
dopamine 3-sulfate
2.1 - 2.8
dopamine 4-sulfate
14.1 - 18
epinephrine 3-sulfate
1.9 - 2.5
epinephrine 4-sulfate
0.344
Estrone 3-sulfate
-
-
1.05
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate
-
-
10.5 - 12.8
norepinephrine 3-sulfate
2.7 - 3.3
norepinephrine 4-sulfate
0.0549 - 8.7
p-Nitrophenyl sulfate
3.65
phloroglucinol monosulfate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000095 - 0.023
4-nitrophenyl phosphate
0.000061 - 90.83
4-nitrophenyl sulfate
1000
p-Nitrophenyl sulfate
Pseudoalteromonas carrageenovora
-
6.5 nM purified arylsulfatase, at 45C and pH 7.0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.302
2-(difluoromethyl)phenyl hydrogen sulfate
-
pH 8.9, 30C
0.975
3,4-dihydro-1,2,3-benzoxathiazine 2,2-dioxide
-
pH 8.9, 30C
0.401
3H-1,2,3-benzoxathiazole 2,2-dioxide
-
pH 8.9, 30C
0.029
4-(difluoromethyl)phenyl hydrogen sulfate
-
pH 8.9, 30C
0.0292
4-nitrophenyl phosphate
-
wild type enzyme, in 100 mM Tris, pH 8.0, 25C
0.0045
phenyl sulfamate
-
pH 8.9, 30C
0.00055
praziquantel
-
pH 6.0, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 2.4
phosphate
0.001 - 2.9
sulfate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.93
-
substrate agar
4.07
-
arylsulfatase B
4.82
-
arylsulfatase A
97.2
-
substrate p-nitrophenyl sulfate
113
-
maximal specific activity using p-nitrophenyl sulfate
468
-
7.1 mM p-nitrophenyl sulfate at 45C and at pH 8.5, after Heparin-Sepharose column chromatography
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 4.7
-
hydrolysis of ascorbic acid 2-sulfate
4.3
-
hydrolysis of 4-nitrocatechol sulfate
5 - 5.6
-
pH optimum gradually changes from pH 5.6 to 5.0 when the time of incubation is prolonged from 5 to 30-120 min
5.2
-
arylsulfatase A
5.5 - 6
-
4-nitrophenyl sulfate
5.8
-
10 min incubation in presence of NaCl
6 - 6.2
-
hydrolysis of 4-methylumbelliferone sulfate
6
-
120 min incubation in presence of K2SO4
6 - 6.5
-
hydrolysis of 2-hydroxy-5-nitrophenyl sulfate and 2-hydroxy-4-nitrophenyl sulfate
6
-
hydrolysis of 2-hydroxy-5-nitrophenyl sulfate in citric acid/sodium citrate buffer
6.1
-
hydrolysis of 4-nitrocatechol sulfate
6.8 - 8.2
-
-
7.4
-
assay at
8.4
-
hydrolysis of 4-nitrophenyl sulfate, beta enzyme; hydrolysis of nitrocatechol sulfate, beta enzyme
8.8
-
hydrolysis of 4-nitrocatechol sulfate, alpha enzyme
9.8
-
without imidazole
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
-
pH 4: about 15% of maximal activity, pH 7.0: about 55% of maximal activity
4 - 6
-
about 45% maximal activity at pH 4 and pH 6, arylsulfatase A; pH 4: about 35% of maximal activity, pH 6.0: about 75% of maximal activity
5 - 7.4
-
50% of maximal activity at pH 5.0 and at pH 7.4, hydrolysis of 4-nitrophenyl sulfate or 4-nitrocatecholsulfate
5 - 7
-
pH 5.0: about 55% of maximal activity, pH 7.0: about 35% of maximal activity, hydrolysis of 4-nitrophenyl sulfate; pH 5.0: about 75% of maximal activity, pH 7.0: about 45% of maximal activity, hydrolysis of 4-nitrocatechol sulfate
5.2 - 7.9
-
pH 5.2: about 70% of maximal activity, pH 7.9: about 50% of maximal activity
7 - 10
7.4 - 10
-
pH 7.4: about 35% of maximal activity, 10.0: about 50% of maximal activity
7.5 - 10.2
-
pH 7.5: about 55% of maximal activity, pH 10.2: about 10% of maximal activity
7.5 - 10
-
pH 7.5: about 30% of maximal activity, pH 10: about 90% of maximal activity, enzyme Es-2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 50
-
ASG cleaves 4-nitrocatechol sulfate most efficiently at 45-50C
additional information
Littorina kurila
-
50% loss in activity at 60C compared to control condition of 37C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
14% residual activity
4 - 50
10 - 60
-
10C: about 35% of maximal activity, 60C: about 30% of maximal activity
40 - 65
-
40C: about 40% of maximal activity, 65C: about 60% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
enzyme expression is unaltered in cancer tissue compared to nonmalignant tissue
Manually annotated by BRENDA team
-
very low enzymic activity at 41 weeks of gestation, which reduce to a half at 42 weeks of gestation, while values of sulfatide concentrations increase
Manually annotated by BRENDA team
-
specific activity of enzyme remains low until testes reach a weight of approximately 1 g and then increases. Testes and epididymal regression induced by a short photoperiod increases enzyme activity in the testes and reduces activity in epididymides
Manually annotated by BRENDA team
-
primary epithelial cell, highest activity of galacose 6-sulfatase and aryl sulfatase B of all the cell lines tested
Manually annotated by BRENDA team
poor expression
Manually annotated by BRENDA team
poor expression
Manually annotated by BRENDA team
-
enzyme expression is unaltered in breast cancer tissue compared to nonmalignant tissue
Manually annotated by BRENDA team
-
remarkably low activity of arylsulfatase A, arylsulfatase B, galacose 6-sulfatase and steryl sulfatase, but not iduronate 2-sulfatase
Manually annotated by BRENDA team
-
primary myoepithelial cell, highest activity of steryl sulfatase and aryl sulfatase B of all the cell lines tested
Manually annotated by BRENDA team
-
localized at sperm head surface. Enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity
Manually annotated by BRENDA team
-
P70 is present on capacitated cauda sperm surface
Manually annotated by BRENDA team
-
activity increases gradually during development
Manually annotated by BRENDA team
-
remarkably low activity of arylsulfatase A, arylsulfatase B, galacose 6-sulfatase and steryl sulfatase, but not iduronate 2-sulfatase
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
P70 is present mainly in the acrosomal soluble fraction
-
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
gel filtration
40000
-
the enzyme has the ability to complex to molecular masses of more than 300000, gel filtration
41000
-
gel permeation chromatography
51000
-
arylsulfatase B, gel filtration
59000
-
enzyme from fraction II, gel filtration
60000
-
SDS-PAGE
62000
-
SDS-PAGE
63000
-
SDS-PAGE
68000
-
immunoblot
70000
-
SDS-PAGE
94000
-
enzyme from fraction I, gel filtration
94900
-
sedimentation equilibrium
97000
-
gel filtration
100000
105000
-
enzyme from fraction III, gel filtration
107000
-
arylsulfatase A, gel filtration
150000
-
gel filtration, ultracentrifugation
166000
-
gel filtration
349000
-
one of two molecular forms consisting of identical subunits with molecular mass of 53000 Da
460000
-
one of two molecular forms consisting of identical subunits with molecular mass of 53000 Da
670000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decamer
-
10 * 63000, SDS-PAGE
dimer
-
2 * 40000, SDS-PAGE
monomer
additional information
-
enzyme has a specific affinity for chondroitin sulfate B, a component of the cumulus matrix proteoglycan network
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no modification
-
not a glycoprotein
proteolytic modification
side-chain modification
-
formylglycine is generated by posttranslational modification of a conserved cysteine residue. Cysteine is also converted to formylglycine when the gene is expressed in Escherichia coli. Substituting the relevant cysteine by a serine codon in the gene of Pseudomonas aeruginosa leads to expression of inactive sulfatase protein, lacking the formylglycine. The machinery catalyzing the modification of the Pseudomonas sulfatase in E. coli therefore accepts cysteine but not serine as a modification substrate
additional information
presence of a disulfide bridge between the processed 18-kDa fragment and another smaller polypeptide, probably involving residue Cys195 of the 10-kDa chain
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals of arylsulfatase are obtained at a pH of 6.3 by hanging drop vapour diffusion method. The three-dimensional structure is determined to 1.3 A resolution in the space group C2. The asymmetric unit contains two monomers
-
full quantum chemical study performed at the density functional level, based on PDB entry 1HDH. The enzyme is able to catalyze the hydrolysis of the original 4-nitrophenyl sulfate substrate and the promiscuous 4-nitrophenyl phosphate.Only some steps of the promiscuous reaction are identical to those in the native process. Differences concern mainly the last step in which the His115 residue acts as a general base to accept the proton by the O atom of the FGly51 in the 4-nitrophenyl sulfate, whereas in 4-nitrophenyl phosphate, the Asp317 protonated residue works as a general acid to deliver a proton by a water molecule to the oxygen atom of the C-O bond
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
below pH 4 in presence of 1% SDS arylsulfatase A is irreversibly converted into inactive subunits
135529
5.5
-
rapid loss of activity below
135535
6
-
rapid inactivation below
135520
6.5 - 10
-
stable
135514
8 - 10
-
stable
135520
8 - 9.5
additional information
-
lowering pH and increasing glycosylation reduces the flexibility of the enzyme. At acidic pH, the glycosylated enzyme presents a higher secondary conformational stability when compared to its nonglycosylated counterpart
730069
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
stable for 2 h
22
-
stable at room temperature
30
-
3 h, stable up to
30 - 40
-
5 min, in presence of substrate, stable
30
-
enzyme Es-2, stable below
37
-
5 h, 30% loss of activity
40
-
30 min, stable below
50
-
30 min, about 50% loss of activity
55
-
ASG activity is retained even at relatively high temperatures above 55C
56
-
5 h, 51% loss of activity
70
-
30 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
several cycles of freezing and thawing cause a marked reduction of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 2 days, 60% loss of activity
-
-20C, 8 weeks, about 80% loss of activity
-
-20C, stable for 2 months
-
-20C, stable for more than 6 months
-
-20C, stable for several months
-
0C, without freezing, 72 h, inactivation
-
3C, 0.1 M sodium phosphate buffer, 14 days, 25% loss of activity
-
4C, pH 6.0, half-life of more than 100 days
-
4C, stable for 24 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
arylsulfatase A and B
-
arylsulfatase Es-1
-
arylsulfatase Es-2
-
DEAE-cellulose anion-exchange and Heparin-Sepharose CL 6B affinity chromatography, all steps at 4C
-
from liver of Schistosoma-infected animal
-
gel filtration on a Sepharose CL 6B column under elution with 0.025 M acetate buffer, pH 5.4
Littorina kurila
-
Ni-NTA column chromatography and HisTrap column chromatography
-
partial
partial, arylsulfatase A and B
-
retroviral expressed enzyme purified with ion-exchange DEAE-cellulose chromatography followed by immuno-affinity purification using a polyclonal antibody against arylsulfatase A sequence
-
strain R-0827, partial, arylsulfatase III
-
strain SH 10-6, partial, arylsulfatase III
-
type II arylsulfatase
-
use of solid phase extraction on a polymeric resin for purification without affecting the enzyme activity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ARS-A and ARS-B, quantitative real-time RT-PCR expression analysis
-
ARSA, cloning of the coding sequence of the human ARSA cDNA tagged with HA epitope in C-terminus into viral serotype 5 adeno-associated vector, AAV2-5
-
ARSA, DNA and amino acid sequence determination and analysis of wild-type and mutant
-
astA gene from Pseudoalteromonas carrageenovora genome is subcloned into pHCEIA vector employing the hyper constitutive expression promoter from the D-amino acid aminotransferase gene of Geobacillus toebii. When the cell is cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reaches 12.8 micromol/min/mL. On MaxyBroth-HD medium with 2% glycerol, the cell shows 2.7fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 micromol/min/mL of arylsulfatase at 20 h, which corresponds to 4fold higher enzyme activity than that of 2% glycerol batch culture
-
expressed in COS-7 cells
-
expressed in HT-1080 cells
-
expression in active form in a sulfatase-deficient strain of Pseudomonas aeruginosa, thereby restoring growth on aromatic sulfates as sole sulfur source, and in Escherichia coli.Cysteine is also converted to formylglycine in Escherichia coli
-
expression in COS-7 cells
-
expression in Escherichia coli
-
expression in HEK-293 cells
expression in HT-1080 cells
overexpression in E.coli BL21
-
overexpression in Escherichia coli BL21
-
retroviral expression system
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzymic activity is detected when microorganisms are grown in sulfate-limiting conditions
no enzymic activity is measured when microorganisms are present in a medium supplemented with inorganic sulfate as sole sulfur source
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C80A
about 20% of wild-type activity at neutral pH, about 5% of wild-type activity at pH optimum
G137A
-
the mutation is associated with X-linked recessive chondrodysplasia punctata
I40S
-
the mutation is associated with X-linked recessive chondrodysplasia punctata
N350S
-
mutant exhibits a consistent degree of unfolding, which may be related to its in vitro reduced stability
P578S
-
the mutation is associated with X-linked recessive chondrodysplasia punctata
T409M
-
the mutation is associated with X-linked recessive chondrodysplasia punctata
W124G
-
naturally occurring missense mutation causing ARSA deficiency, isolated from two Tunisian patients with late infantile metachromatic leukodystrophy, MLD
N215Q
residue Asn215 functions as glycosylation site
N356Q
residue Asn356 functions as glycosylation site
N497Q
mutation of glycosylation site N497 abrogates transport to lysosomes, due to impaired mannose 6-phosphate modification
C51A
-
mutant with strongly reduced activity
C51S
-
mutant with strongly reduced activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
synthesis
-
purification method for removing phytoestrogens and related compounds without affecting the enzyme activity. Use of solid phase extraction on a polymeric resin for purification
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