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Information on EC 3.1.3.86 - phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase and Organism(s) Mus musculus

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IUBMB Comments
This enzyme hydrolyses 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. The enzyme also shows activity toward (PtdIns(1,3,4,5)P4) . The enzyme is involved in several signal transduction pathways in the immune system leading to an adverse range of effects.
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This record set is specific for:
Mus musculus
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
ship-1, ship-2, inpp5d, inppl1, 5'-inositol phosphatase, sh2-containing inositol phosphatase, sh2 domain-containing inositol 5-phosphatase, ptdins(3,4,5)p3 5-phosphatase, phosphatidylinositol 5-phosphatase, pi 5-phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PtdIns(3,4,5)P3 5-phosphatase
-
-
SH2 domain-containing inositol 5-phosphatase 2
-
-
SH2-containing inositol phosphatase
-
-
Shc-associated p145 protein
-
-
SHIP
-
-
SHIP2
-
-
SKIP
-
-
src homology 2 domain-containing inositol 5-phosphatase 2
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-3,4,5-trisphosphate 5-phosphohydrolase
This enzyme hydrolyses 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. The enzyme also shows activity toward (PtdIns(1,3,4,5)P4) [5]. The enzyme is involved in several signal transduction pathways in the immune system leading to an adverse range of effects.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-trisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
dependent on
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
vinexin beta does not affect PtdIns(3,4,5)P3 5-phosphatase activity of SHIP2
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
cell adhesion to collagen-I-coated dishes is decreased in SHIP2-deicient MEF cells compared with wild type cells
physiological function
-
SHIP2 interaction with vinexin alpha promotes the localization of SHIP2 at the periphery of the cells leaving its catalytic site intact. SHIP2 is active both as a PtdIns(3,4,5)P3 5-phosphatase and as a modulator of focal contact formation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SHIP1_MOUSE
1191
0
133542
Swiss-Prot
other Location (Reliability: 3)
SHIP2_MOUSE
1257
0
138973
Swiss-Prot
other Location (Reliability: 5)
OCRL_MOUSE
900
0
104285
Swiss-Prot
other Location (Reliability: 2)
INP5E_MOUSE
647
0
71915
Swiss-Prot
other Location (Reliability: 3)
INP5K_MOUSE
468
0
54159
Swiss-Prot
other Location (Reliability: 4)
FIG4_MOUSE
907
1
103447
Swiss-Prot
other Location (Reliability: 4)
A0A087WPT7_MOUSE
1166
0
128936
TrEMBL
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
145000
-
gel filtration
150000
-
gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
tyrosine-phosphorylated protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression in COS-7 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Paternotte, N.; Zhang, J.; Vandenbroere, I.; Backers, K.; Blero, D.; Kioka, N.; Vanderwinden, J.M.; Pirson, I.; Erneux, C.
SHIP2 interaction with the cytoskeletal protein Vinexin
FEBS J.
272
6052-6066
2005
Mus musculus
Manually annotated by BRENDA team
Damen, J.E.; Liu, L.; Rosten, P.; Humphries, R.K.; Jefferson, A.B.; Majerus, P.W.; Krystal, G.
The 145-kDa protein induced to associate with Shc by multiple cytokines is an inositol tetraphosphate and phosphatidylinositol 3,4,5-triphosphate 5-phosphatase
Proc. Natl. Acad. Sci. USA
93
1689-1693
1996
Mus musculus
Manually annotated by BRENDA team
Ijuin, T.; Takenawa, T.
Regulation of insulin signaling and glucose transporter 4 (GLUT4) exocytosis by phosphatidylinositol 3,4,5-trisphosphate (PIP3) phosphatase, skeletal muscle, and kidney enriched inositol polyphosphate phosphatase (SKIP)
J. Biol. Chem.
287
6991-6999
2012
Mus musculus
Manually annotated by BRENDA team