Information on EC 3.1.3.86 - phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase

Word Map on EC 3.1.3.86
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.86
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-phosphoinositide degradation
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Inositol phosphate metabolism
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SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-3,4,5-trisphosphate 5-phosphohydrolase
This enzyme hydroylses 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. The enzyme also shows activity toward (PtdIns(1,3,4,5)P4) [5]. The enzyme is involved in several signal transduction pathways in the immune system leading to an adverse range of effects.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
African green monkey
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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the enzyme participates in the insulin signalling pathway in vivo
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-triphosphate + phosphate
show the reaction diagram
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?
1-phosphatidyl-1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-trisphosphate + phosphate
show the reaction diagram
substrate of isoform SHIP1 in vitro
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?
1-phosphatidyl-1D-myo-inositol 1,3,4,5-tetraphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-triphosphate + phosphate
show the reaction diagram
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
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?
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-triphosphate + phosphate
show the reaction diagram
inositol 1,3,4,5-tetrakisphosphate + H2O
?
show the reaction diagram
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in vitro substrate of SHIP2
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 1,3,4-trisphosphate + phosphate
show the reaction diagram
O15357, Q92835
substrate of isoform SHIP1 in vitro
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?
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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Zn2+, Ca2+ and Mn2+ do not support enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-bisphosphoglycerate
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4-hydroxymercuribenzoate
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EDTA
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inositol hexakissulfate
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inhibitory at high concentrations
neomycin
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partial inhibition
phosphatidylinositol 4,5-bisphosphate
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strong inhibition at low micromolar concentrations
phosphatidylinositol 4-phosphate
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inhibition at low micromolar concentrations
vanadate
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partial inhibition at millimolar concentrations
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
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in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication
0.1
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
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in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
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calculated from amino acid sequence
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
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cytosol-associated 5-phosphatase, gel filtration
103000
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catalytic part of SHIP2, gel filtration
145000
150000
155000
isoform SHIP2, gel filtration; isoform SHIP2, gel filtration
160000
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full-length enzyme, gel filtration
170000
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phosphorylated protein, gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione-agarose resin column chromatography
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HiTrap S column chromatography, phosphocellulose column chromatography, heparin-HiTrap column chromatography, Q-HiTrap column chromatography, and S-300 gel filtration
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Ni2+-agarose column chromatography
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the enzyme is co-purified with the p85/pll0 form of PI 3-kinase, by Q-Sepharose column chromatography, S-Sepharose column chromatography, hydroxyapatite column chromatography and Mono S column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in COS-7 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alphaF' cells and in COS-7 cells
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expression in COS-7 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is not increased after thrombin stimulation of COS-7 cells
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enzyme activity is stimulated by the macrophage colony-stimulating factor
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the enzyme is stimulated by 50 ng/ml epidermal growth factor
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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SHIP-1 is a risk marker for acute ischemic stroke