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Information on EC 3.1.3.7 - 3'(2'),5'-bisphosphate nucleotidase and Organism(s) Homo sapiens

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.7 3'(2'),5'-bisphosphate nucleotidase
IUBMB Comments
Also acts on 3'-phosphoadenylyl sulfate, and on the corresponding 2'-phosphates.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
papase, pap phosphatase, bpnt1, rv2837c, fiery1, met22, 3'(2'),5'-bisphosphate nucleotidase, gpapp, bpnt-1, bpntase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'(2'),5'-bisphosphate nucleotidase 1
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3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase
-
-
-
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3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
-
-
-
-
3'(2')-phosphoadenosine 5’-phosphate phosphatase
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3'-phosphoadenylylsulfate 3'-phosphatase
-
-
-
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adenosine-3',5'-bisphosphate 3'-phosphohydrolase
-
-
-
-
bisphosphate 3'-nucleotidase
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-
BPNT1
-
-
DPNPase
-
-
-
-
Halotolerance protein tol1
-
-
-
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nucleotidase, phosphoadenylate 3'-
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-
-
-
PAP-phosphatease
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phosphoadenylate 3'-nucleotidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase
Also acts on 3'-phosphoadenylyl sulfate, and on the corresponding 2'-phosphates.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-83-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
inositol-1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
-
-
?
additional information
?
-
-
the enzyme mediates lithium toxicity effects
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
the activity of enzyme is required while 3'-phosphoadenosine 5'-phosphate is a potent inhibitor of a variety of enzymes that use 3'-phosphoadenosine 5'-phosphate as cosubstrate
-
?
additional information
?
-
-
the enzyme mediates lithium toxicity effects
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required, optimal concentration of 1 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
inositol 1,4-bisphosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
adenosine 3',5'-bisphosphate
pH 7.5, 30°C
0.0004
inositol-1,4-bisphosphate
pH 7.5, 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3 - 0.6
Li+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in glioma tissues, phosphocholine(36:1) is not detected and 3'-phosphoadenylate (pAp) content is significantly increased. The gene expression of the 3'-nucleotidase 3'(2'),5'-bisphosphate nucleotidase 1 (BPNT-1) is dramatically downregulated, quantitative real-time polymerase chain reaction (qRT-PCR) expression analysis. Gene expression of enzymes associated with pAp metabolism
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
in glioma tissues, glycerophosphatidylcholine [PC(36:1)] is not detected and 3'-phosphoadenylate (pAp) content is significantly increased. The gene expressions of 3'-nucleotidases inositol monophosphatase (IMPAD-1) and 3'(2'),5'-bisphosphate nucleotidase 1 (BPNT-1)) are dramatically downregulated. Dramatic downregulation of IMPAD-1 and BPNT-1 are the primary cause for pAp dramatic accumulation
physiological function
Gene expression of enzymes associated with 3'-phosphoadenylate (pAp) metabolism, pAp is a toxic by-product
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BPNT1_HUMAN
308
0
33392
Swiss-Prot
Mitochondrion (Reliability: 4)
IMPA3_HUMAN
359
1
38681
Swiss-Prot
Secretory Pathway (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of HsPIP and expression in Escherichia coli
expression in Sf9 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in glioma tissues, glycerophosphatidylcholine [PC(36:1)] is not detected and 3'-phosphoadenylate content is significantly increased. The gene expression of the 3'-nucleotidase 3'(2'),5'-bisphosphate nucleotidase 1 (BPNT-1) is dramatically downregulated
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
enzyme as a potential target of lithium therapy
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Spiegelberg, B.D.; Xiong, J.P.; Smith, J.J.; Gu, R.F.; York, J.D.
Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3-nucleotidase inhibited by inositol 1,4-bisphosphate
J. Biol. Chem.
274
13619-13628
1999
Homo sapiens (O95861), Homo sapiens, Mus musculus (Q9Z0S1), Mus musculus
Manually annotated by BRENDA team
Yenush, L.; Belles, J.M.; Lopez-Coronado, J.M.; Gil-Mascarell, R.; Serrano, R.; Rodriguez, P.L.
A novel target of lithium therapy
FEBS Lett.
467
321-325
2000
Homo sapiens (O95861), Homo sapiens
Manually annotated by BRENDA team
Spiegelberg, B.D.; Dela Cruz, J.; Law, T.H.; York, J.D.
Alteration of lithium pharmacology through manipulation of phosphoadenosine phosphate metabolism
J. Biol. Chem.
280
5400-5405
2005
Homo sapiens
Manually annotated by BRENDA team
Li, W.; Jia, H.; Li, Q.; Cui, J.; Li, R.; Zou, Z.; Hong, X.
Glycerophosphatidylcholine PC(36 1) absence and 3'-phosphoadenylate (pAp) accumulation are hallmarks of the human glioma metabolome
Sci. Rep.
8
14783
2018
Homo sapiens (O95861), Homo sapiens
Manually annotated by BRENDA team