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Information on EC 3.1.3.62 - multiple inositol-polyphosphate phosphatase and Organism(s) Homo sapiens

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IUBMB Comments
This enzyme exists in two isoforms. It also acts on Ins(1,3,4,5)P4 to yield Ins(1,4,5)P3.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
multiple inositol-polyphosphate phosphatase, (mmu)minpp1, (hsa)minpp1, taphyiia2, taphyiib, hvphyiib, hvphyiia2, inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(HSA)MINPP1
-
1D-myo-inositol-1,3,4,5-tetrakisphosphate 3-phosphohydrolase
-
-
-
-
inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase
-
-
-
-
inositol 1,3,4,5-tetrakisphosphate 3-phosphomonoesterase
-
-
-
-
inositol 1,3,4,5-tetrakisphosphate-5-phosphomonoesterase
-
-
-
-
inositol tetrakisphosphate phosphomonoesterase
-
-
-
-
inositol-1,3,4,5-tetrakisphosphate 3-phosphatase
-
-
-
-
Minpp
-
-
-
-
multiple inositol polyphosphate phosphatase
-
-
phosphatase, inositol 1,3,4,5-tetrakisphosphate 3-
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-hexakisphosphate 5-phosphohydrolase
This enzyme exists in two isoforms. It also acts on Ins(1,3,4,5)P4 to yield Ins(1,4,5)P3.
CAS REGISTRY NUMBER
COMMENTARY hide
116958-30-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-bisphosphoglycerate + H2O
2-phosphoglycerate + phosphate
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1,2,3,4,5,6-hexakisphosphate + H2O
D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + phosphate
show the reaction diagram
-
-
?
inositol 1,3,4,5,6-pentakisphosphate + H2O
inositol 1,4,5,6-tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
catalytic requirement for the C-terminus and His-370 of MIPP
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Mg2+-independent
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.61
2,3-bisphosphoglycerate
-
recombinant enzyme, at 37°C
0.0016
D-myo-Inositol 1,3,4,5-tetrakisphosphate
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.009
-
using 2,3-bisphosphoglycerate as substrate, at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
fetal growth plate cartilage, mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
2.5 kb mRNA expression
Manually annotated by BRENDA team
2.5 kb mRNA expression
Manually annotated by BRENDA team
2.5 kb mRNA expression
Manually annotated by BRENDA team
additional information
not expressed in peripheral blood leukocytes
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MINP1_HUMAN
487
0
55051
Swiss-Prot
Secretory Pathway (Reliability: 5)
B2R7D2_HUMAN
487
0
55120
TrEMBL
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis
53400
x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis
55000
-
deglycosylated recombinant protein, SDS-PAGE
55100
x * 55100, inclusive the putative signal sequence, amino acid sequence analysis
70000
-
glycosylated recombinant protein, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H370A
mutant with less than 5% of activity of wild-type enzyme
H89A
-
catalytically compromised mutant of MIPP1 that shows only 1% of the 2,3-diphosphoglycerate phosphatase activity of the wild type enzyme
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.4
-
MIPP1 activity decreases 50% when pH rises from 7.0 to 7.4
694894
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
-
MIPP1 is active at 4°C, albeit considerably less active than at 37°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose column chromatography
-
recombinant MIPP expressed in Escherichia coli M15
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris
-
full-length Minpp cDNA is cloned and stably overexpressed in murine ATDC-5 cells at 2fold higher levels than in wild-type ATDC-5 cells, cellular effects of overexpression
-
His-tag fusion, expression in Pichia pastoris
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located on chromosome 10q23, genomic organization
MIPP cDNA from Jurkat T-cells is cloned and expressed in Escherichia coli M15 without the ER-targeting domain, full-length sequence
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
Ni-affinity chromatography
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hidaka, K.; Kanematsu, T.; Caffrey, J.J.; Takeuchi, H.; Shears, S.B.; Hirata, M.
The importance to chondrocyte differentiation of changes in expression of the multiple inositol polyphosphate phosphatase
Exp. Cell Res.
290
254-264
2003
Homo sapiens, Mus musculus, Oryctolagus cuniculus
Manually annotated by BRENDA team
Caffrey, J.J.; Hidaka, K.; Matsuda, M.; Hirata, M.; Shears, S.B.
The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification
FEBS Lett.
442
99-104
1999
Homo sapiens (Q9UNW1), Rattus norvegicus
Manually annotated by BRENDA team
Chi, H.; Tiller, G.E.; Dasouki, M.J.; Romano, P.R.; Wang, J.; O'Keefe, R.J.; Puzas, J.E.; Rosier, R.N.; Reynolds, P.R.
Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19
Genomics
56
324-336
1999
Homo sapiens (Q9UNW1), Homo sapiens, Mus musculus (Q9Z2L6), Mus musculus
Manually annotated by BRENDA team
Deleu, S.; Choi, K.; Pesesse, X.; Cho, J.; Sulis, M.L.; Parsons, R.; Shears, S.B.
Physiological levels of PTEN control the size of the cellular Ins(1,3,4,5,6)P5 pool
Cell. Signal.
18
488-498
2006
Homo sapiens
Manually annotated by BRENDA team
Cho, J.; King, J.S.; Qian, X.; Harwood, A.J.; Shears, S.B.
Dephosphorylation of 2,3-bisphosphoglycerate by MIPP expands the regulatory capacity of the Rapoport-Luebering glycolytic shunt
Proc. Natl. Acad. Sci. USA
105
5998-6003
2008
Dictyostelium discoideum, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team