Information on EC 3.1.3.4 - phosphatidate phosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.3.4
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RECOMMENDED NAME
GeneOntology No.
phosphatidate phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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diacylglycerol and triacylglycerol biosynthesis
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Ether lipid metabolism
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Glycerolipid metabolism
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Glycerophospholipid metabolism
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Metabolic pathways
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plasmalogen biosynthesis
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Sphingolipid metabolism
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sphingosine metabolism
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stigma estolide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
diacylglycerol-3-phosphate phosphohydrolase
This enzyme catalyses the Mg2+-dependent dephosphorylation of a 1,2-diacylglycerol-3-phosphate, yielding a 1,2-diacyl-sn-glycerol (DAG), the substrate for de novo lipid synthesis via the Kennedy pathway and for the synthesis of triacylglycerol. In lipid signalling, the enzyme generates a pool of DAG to be used for protein kinase C activation. The mammalian enzymes are known as lipins.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-77-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
isozymes LPP1, LPP-2, and LPP-3
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain T-85
UniProt
Manually annotated by BRENDA team
strain T-85
UniProt
Manually annotated by BRENDA team
Mus musculus C57BL/6J
C57BL/6J mice
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cv. 1183
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol 3-phosphate + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
-
?
1,2-diacyl-sn-glycerol-3-phosphate + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
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-
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1,2-dioleoyl-sn-glycerol 3-phosphate + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
-
?
1,2-dipalmitoyl-sn-glycerol 3-phosphate + H2O
1,2-dipalmitoyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
-
?
1-acyl-sn-glycerol 3-phosphate + H2O
1-acyl-sn-glycerol + phosphate
show the reaction diagram
1-arachidonoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-arachidonoyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
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?
1-arachidonoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-arachidonoylglycerol + phosphate
show the reaction diagram
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-
-
?
1-hexanoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphate + H2O
1-hexanoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycerol + phosphate
show the reaction diagram
1-lauroyl-2-lysophosphatidate + H2O
1-lauroyl-sn-glycerol + phosphate
show the reaction diagram
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-
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?
1-myristoyl-2-lysophosphatidate + H2O
1-myristoyl-sn-glycerol + phosphate
show the reaction diagram
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-
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?
1-myristoyl-sn-glycerol 3-phosphate + H2O
1-myristoylglycerol + phosphate
show the reaction diagram
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?
1-oleoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-oleoyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
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?
1-oleoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-oleoylglycerol + phosphate
show the reaction diagram
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?
1-oleoyl-2-lysophosphatidate + H2O
oleic acid + sn-glycerol 3-phosphate
show the reaction diagram
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?
1-palmitoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-palmitoyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
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?
1-palmitoyl-2-lysophosphatidate + H2O
1-palmitoyl-sn-glycerol + phosphate
show the reaction diagram
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?
1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate + H2O
1-stearoyl-2-arachidonoyl-sn-glycerol + phosphate
show the reaction diagram
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?
1-stearoyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-stearoyl-sn-glycerol + phosphate
show the reaction diagram
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?
1-stearoyl-2-lysophosphatidate + H2O
1-stearoyl-sn-glycerol + phosphate
show the reaction diagram
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?
1-stearyl-2-lyso-sn-glycerol 3-phosphate + H2O
1-stearylglycerol + phosphate
show the reaction diagram
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?
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphate + H2O
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycerol + phosphate
show the reaction diagram
5'-AMP + H2O
?
show the reaction diagram
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8.6% of the activity with phosphatidic acid
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?
a 3-sn-phosphatidate + H2O
a 1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
ATP + H2O
?
show the reaction diagram
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7.2% of the activity with phosphatidic acid
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?
attophos A + H2O
?
show the reaction diagram
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?
beta-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
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about 10% of the activity with phosphatidic acid
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?
ceramide 1-phosphate + H2O
ceramide + phosphate
show the reaction diagram
ceramide-1-phosphate + H2O
?
show the reaction diagram
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isoenzyme PAP-2a and PAP-2b
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?
diacylglycerol diphosphate + H2O
?
show the reaction diagram
diacylglycerol diphosphate + H2O
? + phosphate
show the reaction diagram
diacylglycerol diphosphate + H2O
phosphatidate + phosphate
show the reaction diagram
dicaproyl phosphatidate + H2O
1,2-dicaproyl-sn-glycerol + phosphate
show the reaction diagram
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best substrate of the 104-kDa enzyme form
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?
didecanoyl phosphatidic acid + H2O
1,2-didecanoyl-sn-glycerol + phosphate
show the reaction diagram
dihexanoyl phosphatidic acid + H2O
1,2-dihexanoyl-sn-glycerol + phosphate
show the reaction diagram
dihydro-sphingosine-1-phosphate + H2O
dihydro-sphingosine + phosphate
show the reaction diagram
dimyristoyl phosphatidic acid + H2O
1,2-dimyristoyl-sn-glycerol + phosphate
show the reaction diagram
dioctanoyl phosphatidic acid + H2O
1,2-dioctanoyl-sn-glycerol + phosphate
show the reaction diagram
dioleoyl phosphatidate + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
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?
dioleoyl phosphatidic acid + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
dipalmitoyl phosphatidate + H2O
1,2-dipalmitoyl-sn-glycerol + phosphate
show the reaction diagram
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?
dipalmitoyl phosphatidic acid + H2O
?
show the reaction diagram
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?
distearoyl phosphatidic acid + H2O
1,2-distearoyl-sn-glycerol + phosphate
show the reaction diagram
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?
DL-alpha-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
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about 10% of the activity with phosphatidic acid
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?
FTY720-phosphate + H2O
FTY720 + phosphate
show the reaction diagram
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
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?
lyso-phosphatidic acid + H2O
monoacylglycerol + phosphate
show the reaction diagram
lysophosphatidate + H2O
?
show the reaction diagram
lysophosphatidate + H2O
monoacylglycerol + phosphate
show the reaction diagram
lysophosphatidic acid + H2O
?
show the reaction diagram
lysophosphatidic acid + H2O
? + phosphate
show the reaction diagram
lysophosphatidic acid + H2O
mono-oleoyl-glycerol + phosphate
show the reaction diagram
lysophosphatidic acid + H2O
monoacylglycerol + phosphate
show the reaction diagram
octanoyl lysophosphatidic acid + H2O
?
show the reaction diagram
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?
oleoyl phosphatidic acid + H2O
?
show the reaction diagram
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?
p-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
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about 10% of the activity with phosphatidic acid
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?
phosphatidate + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidate + H2O
diacyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-diacylglycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-sn-diacylglycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
?
show the reaction diagram
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?
phosphatidic acid + H2O
? + phosphate
show the reaction diagram
phosphatidic acid + H2O
diacylglycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
phosphate + diacylglycerol
show the reaction diagram
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?
sphingosine 1-phosphate + H2O
?
show the reaction diagram
sphingosine 1-phosphate + H2O
sphingosine + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a 3-sn-phosphatidate + H2O
a 1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
diacylglycerol diphosphate + H2O
phosphatidate + phosphate
show the reaction diagram
lysophosphatidate + H2O
monoacylglycerol + phosphate
show the reaction diagram
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best substrate
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?
lysophosphatidic acid + H2O
monoacylglycerol + phosphate
show the reaction diagram
phosphatidate + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
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biochemical regulation of PA phosphatases involving phospholipids, nucleotides ATP and CTP and the cAMP-dependent protein kinase A, phosphorylation does not affect substrate binding but does alter the catalytic step in the reaction, overview, PA phosphatase activity is regulated by biochemical and genetic mechanisms in a reciprocal manner with the regulation of the phospholipid biosynthetic enzyme phosphatidylserin synthase, overview
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?
phosphatidate + H2O
diacyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-diacyl-sn-glycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-diacylglycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
Q91ZP3
insulin and epinephrine control lipin 1 primarily by changing localization rather than intrinsic PAP activity, overview
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?
phosphatidic acid + H2O
1,2-sn-diacylglycerol + phosphate
show the reaction diagram
phosphatidic acid + H2O
diacylglycerol + phosphate
show the reaction diagram
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?
sphingosine 1-phosphate + H2O
sphingosine + phosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co(NO3)2
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0.3 mM, about 3fold stimulation
Co2+
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stimulates
Fe2+
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stimulates
Fe3+
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stimulates
MnSO4
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0.3 mM, about 3fold stimulation
ZnSO4
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0.3 mM, about 2.5fold stimulation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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1,2-diacylglycerol
1-arachidonoyl-2-lyso-sn-glycerol 3-phosphate
32% inhibition
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1-myristoyl-2-lyso-sn-glycerol 3-phosphate
63% inhibition
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1-oleoyl-2-lyso-sn-glycerol 3-phosphate
24% inhibition
1-palmitoyl-2-lyso-sn-glycerol 3-phosphate
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40 microM, about 50% inhibition
2,2'-dithiodipyridine
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9 mM, 90% inhibition of Mg2+-dependent enzyme, 16% inhibition of Mg2+-independent enzyme
4,4'-dithiodipyridine
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9.0 mM, 96% inhibition of Mg2+-dependent enzyme, 20% inhibition of Mg2+-independent enzyme
4-chlormercuriphenylsulfonic acid
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5,5'-dithiobis(2-nitrobenzoic acid)
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0.2 mM, 80% inhibition of Mg2+-dependent enzyme, no inhibition of Mg2+-independent enzyme
amphiphilic amines
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Be2+
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bromoenol lactone
Butane-2,3-dione
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ceramide 1-phosphate
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chlorpromazine
cystamine dihydrochloride
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1 mM, 25% inhibition of Mg2+-dependent enzyme, 8% inhibition of Mg2+-independent enzyme
diacylglycerol
diacylglycerol diphosphate
dihydrosphingosine
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dimethylsphingosine
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DMSO
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addition to the reaction mixture results in a dose-dependent inhibition of PAP1 activity, 25% loss of PAP1 activity at a 1% concentration
dodecyl phosphate
99% inhibition
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