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IUBMB CommentsDoes not act on nucleoside monophosphates. Induced in Escherichia coli by T-even phages.
The enzyme appears in viruses and cellular organisms
Synonyms
mrna 5'-triphosphatase, polynucleotide 5'-triphosphatase,
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5'-polynucleotidase
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mRNA 5'-triphosphatase
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phosphatase, polynucleotide 5-
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Polynucleotide 5'-triphosphatase
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a 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate
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hydrolysis of phosphoric ester
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polynucleotide 5'-phosphohydrolase
Does not act on nucleoside monophosphates. Induced in Escherichia coli by T-even phages.
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5'-phosphyl-terminated DNA + H2O
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5'-phosphyl-terminated DNA + H2O
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CoCl2
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divalent metal required, 30% the rate observed with MgCl2
MgCl2
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divalent metal required
MnCl2
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divalent metal required, 60% the rate observed with MgCl2
additional information
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no cleavage in absence of divalent metal ion
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p-hydroxymercuribenzoate
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in absence of sulfhydryl compounds
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Vaccinia
Purification and characterization of core-associated polynucleotide 5'-triphosphatase from Vaccinia virus.
Vaccinia
Synthesis of polynucleotide 5'-triphosphatase in vaccinia virus-infected HeLa cells.
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5.5 - 6.8
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50% of maximal activity at pH 5.5 and pH 6.8
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B, induced by T4am82
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Becker, A.; Hurwitz, J.
The enzymatic cleavage of phosphate termini from polynucleotides
J. Biol. Chem.
242
936-950
1967
Escherichia coli
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