Information on EC 3.1.3.1 - alkaline phosphatase

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.3.1
-
RECOMMENDED NAME
GeneOntology No.
alkaline phosphatase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
phosphomonoester
-
PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
diethylphosphate degradation
-
Folate biosynthesis
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
phosphate-monoester phosphohydrolase (alkaline optimum)
Wide specificity. Also catalyses transphosphorylations. The human placental enzyme is a zinc protein. Some enzymes hydrolyse diphosphate (cf. EC 3.6.1.1 inorganic diphosphatase)
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alkaline phenyl phosphatase
-
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Q9KWY4
-
alkaline phosphatase
-
-
alkaline phosphatase
P19111
-
alkaline phosphatase
A3ZF85
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
P00634
-
alkaline phosphatase
-
-
alkaline phosphatase
Q92058
-
alkaline phosphatase
C1K6P2
-
alkaline phosphatase
A8WEG4
-
alkaline phosphatase
-
-
alkaline phosphatase
B0R9W3
-
alkaline phosphatase
Halomonas sp.
-
-
alkaline phosphatase
B4Z1D6, B4Z1D7
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Neurospora crassa St. L. 74A
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Q17TZ1
-
alkaline phosphatase
-
-
alkaline phosphatase
Q9UZV2
-
alkaline phosphatase
P08289
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Saccharomyces cerevisiae 257
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Serratia marcescens 211
-
-
-
alkaline phosphatase
shrimp
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
A1YYW7
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Terfezia claveryi Chatin
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Thermus sp. 314
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Ulva pertusa Kjelm
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase
Vibrio sp. 2P44
-
-
-
alkaline phosphatase
-
-
alkaline phosphatase F I
-
-
alkaline phosphatase F II
-
-
alkaline phosphatase HaALP1
B4Z1D6
-
alkaline phosphatase HaALP2
B4Z1D7
-
alkaline phosphatase HvmALP 1
C3U1V9
-
alkaline phosphatase HvmALP 2
C3U1W0
-
alkaline phosphatase HvmALP 3
C3U1W1
-
alkaline phosphatase HvmALP 4
C3U1W2
-
alkaline phosphatase HvmALP 5
B4X983
-
alkaline phosphatase VI-1
-
-
alkaline phosphohydrolase
-
-
-
-
alkaline phosphomonoesterase
-
-
-
-
all2843
-
-
ALP
-
-
-
-
ALP
P19111
-
ALP
Q17TZ1
-
ALP
-
bone isoform; intestinal isoform; liver isoform
ALP
P08289
liver isoform
ALP
Ulva pertusa Kjelm
-
-
-
ALPase
-
-
ALPase
Saccharomyces cerevisiae 257
-
-
-
ALPP
-
-
alr5291
-
alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs
AP
shrimp
-
-
AP
Thermus sp. 314
-
-
-
AP-TNAP
-
-
-
-
APase
-
-
-
-
APase
-
-
APase
Serratia marcescens 211
-
-
-
APASED
-
-
-
-
atypical purple alkaline phosphatase
B4EKR2
-
BALP
-
-
BC6
-
-
-
-
BIALP
-
-
BIAP
-
-
bone alkaline phosphatase
-
-
bone specific alkaline phosphatase
-
-
calf intestinal alkaline phosphatase
-
-
CAP
-
-
CAPase
Serratia marcescens 211
-
-
-
CIAP
-
-
EAP
-
-
-
-
extracellular alkaline phosphatase
Chenopodium rubrum
-
-
germ cell alkaline phosphatase
-
-
Germ-cell alkaline phosphatase
-
-
-
-
glycerophosphatase
-
-
-
-
H-AP
-
-
-
-
H-AP
-
high-molecular weight alkaline phosphatase
H-AP
Pseudomonas aeruginosa H103
-
high-molecular weight alkaline phosphatase
-
HaALP
Halomonas sp.
-
-
HaALP
Halomonas sp. 593
-
-
-
HaALP
B4Z1D6, B4Z1D7
-
high molecular weight alkaline phosphatase
-
-
High molecular weight phosphatase
-
-
-
-
IAP
-
-
-
-
IAPase
Serratia marcescens 211
-
-
-
intestinal alkaline phosphatase
P19111
-
intestinal alkaline phosphatase
-
-
intestinal alkaline phosphatase
-
-
L-AP
-
-
-
-
L-AP
-
low-molecular weight alkaline phosphatase
L-AP
Pseudomonas aeruginosa H103
-
low-molecular weight alkaline phosphatase
-
Liver/bone/kidney isozyme
-
-
-
-
low molecular weight alkaline phosphatase
-
-
Low molecular weight phosphatase
-
-
-
-
M-ALP
-
-
-
-
Nagao isozyme
-
-
-
-
Nl-ALP1
-
soluble protein
Nl-ALP2
-
insoluble memrane-bound protein
non-specific alkaline phosphatase
-
-
-
-
OE5192R
B0R9W3
gene coding for HSAP
orthophosphoric-mono phosphohydrolase
-
-
PALP
-
-
PHO13
-
gene name
PHO8
-
gene name
phosphatase, alkaline
-
-
-
-
phosphomonoesterase
-
-
-
-
phox
Pasteurella multocida X-73
A1C3J6
-
-
placental alkaline phosphatase
-
-
PLAP-like
-
-
-
-
purple acid phosphatase-like protein
B4EKR2
-
RAN1
-
-
-
-
Regan isozyme
-
-
-
-
rTL2
P08289
-
SAP
shrimp
-
-
SEAP
-
-
secreted alkaline phosphatase
-
-
semen plasma alkaline phosphatase
-
-
seminal plasma alkaline phosphatase
-
-
seminal plasma AP
-
-
SmAP
A8TKU6
-
TAB5 AP
Q9KWY4
-
tissue non-specific alkaline phosphatase
-
-
tissue nonspecific alkaline phosphatase
-
-
tissue-nonspecific alkaline phosphatase
P09487
-
tissue-nonspecific alkaline phosphatase
-
-
tissue-nonspecific alkaline phosphatase
-
-
tissue-nonspecific alkaline phosphatase
P05186
-
tissue-nonspecific alkaline phosphatase
-
-
tissue-nonspecific ALP
-
-
tissue-specific intestinal ALP type II
-
-
TNAP
-
-
TNAP
P09487
-
TNAP
P05186
-
TNAP
-
-
TNSALP
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9001-78-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain K1
-
-
Manually annotated by BRENDA team
antarctic bacterium
strain HK47
-
-
Manually annotated by BRENDA team
antarctic bacterium
strain TAB5
-
-
Manually annotated by BRENDA team
antarctic bacterium HK47
strain HK47
-
-
Manually annotated by BRENDA team
three isoenzymes: I, II, III
-
-
Manually annotated by BRENDA team
alkaline phosphatase F I; alkaline phosphatase F II
-
-
Manually annotated by BRENDA team
Bacillus licheniformis MC14
strain MC14
-
-
Manually annotated by BRENDA team
strain P9
-
-
Manually annotated by BRENDA team
Bacillus sp. OK-1
-
-
-
Manually annotated by BRENDA team
strain P9
-
-
Manually annotated by BRENDA team
2 isoenzymes from calf: C1 and C2, one isoenzyme from adult B2; calf
-
-
Manually annotated by BRENDA team
strain J2315
UniProt
Manually annotated by BRENDA team
CBS 6947
-
-
Manually annotated by BRENDA team
Candida tropicalis CBS 6947
CBS 6947
-
-
Manually annotated by BRENDA team
beagle and mixed breed, 2 genes encoding 2 isozyme types: a tissue non-specific isozyme and an intestinal isozyme
-
-
Manually annotated by BRENDA team
Chenopodium rubrum
-
-
-
Manually annotated by BRENDA team
strain KMM MC-296
-
-
Manually annotated by BRENDA team
Cobetia marina KMM MC-296
strain KMM MC-296
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
gene alp
-
-
Manually annotated by BRENDA team
stallion
-
-
Manually annotated by BRENDA team
gene phoA
-
-
Manually annotated by BRENDA team
gene phoA
UniProt
Manually annotated by BRENDA team
gene phoA, 3 isozymes 1,2, and 3 differing in the N-terminal sequence; gene phoA, 3 isozymes 1, 2, and 3 differing in the N-terminal sequence
-
-
Manually annotated by BRENDA team
K12 strain 706, a periplasmic-excretory mutant
-
-
Manually annotated by BRENDA team
several isoenzymes
-
-
Manually annotated by BRENDA team
strain C4F1
-
-
Manually annotated by BRENDA team
Escherichia coli C4F1
strain C4F1
-
-
Manually annotated by BRENDA team
Halomonas sp.
-
-
-
Manually annotated by BRENDA team
Halomonas sp.
strain 593
-
-
Manually annotated by BRENDA team
Halomonas sp. 593
strain 593
-
-
Manually annotated by BRENDA team
4 isozyme types: 1. nonspecific liver/bone/kidney, which is also present in osteoblast and neutrophilic granulocytes, 2. intestinal, 3. placental, 4. germ-cell
-
-
Manually annotated by BRENDA team
at least 3 isoenzymes: placental, intestinal and tissue non-specific
-
-
Manually annotated by BRENDA team
humanplacenta alkaline phosphatase PLAP
Uniprot
Manually annotated by BRENDA team
precursor
UniProt
Manually annotated by BRENDA team
secreted alkaline phosphatase SEAP containing a single N-glycan chain, a model protein for investigation of glycosylation pattern
-
-
Manually annotated by BRENDA team
several isozymes
-
-
Manually annotated by BRENDA team
strain KP1
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae KP1
strain KP1
-
-
Manually annotated by BRENDA team
Micrococcus sodonensis
-
-
-
Manually annotated by BRENDA team
C57BL/6-N mice
-
-
Manually annotated by BRENDA team
four-days-old ICR mice
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6-N
C57BL/6-N mice
-
-
Manually annotated by BRENDA team
levamisole-insensitive alkaline phosphatase activity; levamisole-sensitive alkaline phosphatase activity
-
-
Manually annotated by BRENDA team
strain St. L. 74A, constitutive enzyme expression
-
-
Manually annotated by BRENDA team
wild-type strain osmotically-sensitive mutant os-1
Q7S2X3
SwissProt
Manually annotated by BRENDA team
Neurospora crassa St. L. 74A
strain St. L. 74A, constitutive enzyme expression
-
-
Manually annotated by BRENDA team
no activity in Marinovum algicola
-
-
-
Manually annotated by BRENDA team
no activity in Marinovum algicola ATCC 51442
-
-
-
Manually annotated by BRENDA team
Ophicephalus punctatus Bloch
-
-
-
Manually annotated by BRENDA team
a major intestinal-like isoenzyme and a minor tissue-unspecific type
-
-
Manually annotated by BRENDA team
strain X-73
UniProt
Manually annotated by BRENDA team
Pasteurella multocida X-73
strain X-73
UniProt
Manually annotated by BRENDA team
Porphyromonas gingivalis 381
strain 381
-
-
Manually annotated by BRENDA team
ATCC 25611
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa H103
H103
-
-
Manually annotated by BRENDA team
euryarchaeon, strain Orsay, highly thermostable enzyme
SwissProt
Manually annotated by BRENDA team
several intestinal isozymes
-
-
Manually annotated by BRENDA team
streptozotocin-induced diabetic rat
-
-
Manually annotated by BRENDA team
three subforms: 1, 2 and 3
-
-
Manually annotated by BRENDA team
substrate specific enzyme form, non specific enzyme form from structural gene PHO8, and particulate enzyme form from structural gene PHO8
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae 257
strain 257
-
-
Manually annotated by BRENDA team
Saccharomyces pombe
substrate specific enzyme form from structural gene PHO2, non specific enzyme form from structural gene PHO3 and particulate enzyme form from structural gene PHO3
-
-
Manually annotated by BRENDA team
clam, Da Costa 1778
-
-
Manually annotated by BRENDA team
thermostable conidial and mycelial alkaline phosphatases, thermophilic fungus
-
-
Manually annotated by BRENDA team
strain 211, 2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-
-
Manually annotated by BRENDA team
Serratia marcescens 211
strain 211, 2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-
-
Manually annotated by BRENDA team
shrimp
cold-adapted enzyme
-
-
Manually annotated by BRENDA team
domestica
-
-
Manually annotated by BRENDA team
Synechococcus elongatus PCC 7942 R2
strain R2
-
-
Manually annotated by BRENDA team
Terfezia claveryi Chatin
Chatin
-
-
Manually annotated by BRENDA team
strain DSM 5068
-
-
Manually annotated by BRENDA team
strain 314
-
-
Manually annotated by BRENDA team
strain T351
SwissProt
Manually annotated by BRENDA team
Thermus sp. 314
strain 314
-
-
Manually annotated by BRENDA team
strain T351
SwissProt
Manually annotated by BRENDA team
strain XM
SwissProt
Manually annotated by BRENDA team
Thermus thermophilus XM
strain XM
SwissProt
Manually annotated by BRENDA team
Kjelm
-
-
Manually annotated by BRENDA team
Ulva pertusa Kjelm
Kjelm
-
-
Manually annotated by BRENDA team
Vibrio 2P44
-
-
Manually annotated by BRENDA team
Vibrio sp. 2P44
Vibrio 2P44
-
-
Manually annotated by BRENDA team
venomous snake
-
-
Manually annotated by BRENDA team
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3810
-
4-nitrophenyl phosphate
P00634
single-chain Fv antibody fusion protein
4742
40.8
-
O-phosphoserine
B4EKR2, -
-
14721
171
-
O-Phosphotyrosine
B4EKR2, -
-
14722
448
-
p-nitrophenyl phosphate
B4EKR2, -
-
15065
94.4
-
phosphoenolpyruvate
B4EKR2, -
-
15572
PDB
SCOP
CATH
ORGANISM
Bacillus subtilis (strain 168)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Pseudomonas fluorescens (strain Pf0-1)
Pseudomonas fluorescens (strain Pf0-1)
Pseudomonas fluorescens (strain Pf0-1)
Pseudomonas fluorescens (strain Pf0-1)
Pseudomonas fluorescens (strain Pf0-1)
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
refolding behavior of guanidine hydrochloride-denatured alkaline phosphatase, despite the enhanced activity recovery yield (more than 50%), both the refolding and the reactivation rate constants decrease with increasing alpha-cyclodextrin concentrations. Detergents (used in the artificial chaperone method) increase the refolding rate significantly
-
recombinant His-tagged enzyme from inclusion bodies after expression in Escherichia coli, solubilization by 1% SDS in ddH2O from purified inclusion bodies
-
incubation in 4 M urea followed by a 15fold dilution of the urea at pH 7.5 results in a 100% recovery of initial activity after 20 or 90 min
-