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bis(p-nitrophenyl) phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate:
-
slow hydrolysis at optimal pH from 5.6 to 5.9
-
?
calf thymus DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
?
chromatin + H2O
oligonucleotides + ?
deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O
p-nitrophenol + deoxyribonucleoside 5'-phosphates
-
-
-
?
DNA + H2O
3' phosphooligonucleotides
-
-
-
?
DNA + H2O
3'-phosphooligonucleotide
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
DNA + H2O
oligonucleotides + ?
dsDNA + H2O
oligonucleotides + ?
-
velocity of DNA degradtion is four times higher four dsDNA than for ssDNA
-
-
?
duplex-oligonucleotide + H2O
?
-
20- and 16mers with specific sequences and structures like hairpin and duplex DNA, can cut bonds in a loop at nearly the same rate as in duplex DNA, doesn't require phosphate exposure or a double-stranded track
-
?
d[ApAp(S)ApA] + H2O
d[Ap(S)ApA] + 3'-dAMP
-
digestion of Rp- and Sp-isomers
after 2 h incubation, prolonged incubation leads to d[Ap(S)Ap]-Rp-isomer, dA and d[Ap(S)Ap]-Sp-isomer. Does not hydrolyse phosphorothioate internucleotidic linkage of either configuration
?
d[ApApApA] + H2O
d[ApApA] + 3'-dAMP
-
-
after 60 min of incubation, prolonged incubation leads to 3'-dAMP and dA end products in a 3 : 1 ratio
?
linearized plasmid DNA + H2O
oligonucleotides + ?
-
-
-
?
nuclei + H2O
oligonucleotides + ?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
salmon testicular DNA + H2O
?
-
-
-
?
ssDNA + H2O
oligonucleotides + ?
-
velocity of DNA degradtion is four times higher four dsDNA than for ssDNA
-
-
?
supercoiled plasmid DNA + H2O
linearized plasmid DNA + ?
-
-
-
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
additional information
?
-
chromatin + H2O
oligonucleotides + ?
-
chicken erythrocyte and yeast nuclei
100 bp cleavage-pattern in the condensed form and 200 bp cleavage-pattern in the extended form
?
chromatin + H2O
oligonucleotides + ?
-
SDS and proteinase K treated substrate
single-stranded regions in oligonucleotides are formed, not detected in untreated chromatin
?
chromatin + H2O
oligonucleotides + ?
-
mouse liver chromatin
100 bp cleavage-pattern of H1-reconstituted chromatin, 200 bp cleavage-pattern at the internucleosomal cleavage site of H1-free chromatin
?
DNA + H2O
3'-phosphooligonucleotide
-
degradation of the DNA of apoptotic cells
-
?
DNA + H2O
3'-phosphooligonucleotide
-
using TdT-mediated dUTP-nicked-end labelling, TUNEL, is followed DNA fragmentation that occurs in vivo during programmed cell death, Nuc-1 functions in the elimination of TUNEL-reactive DNA ends to a TUNEL nonreactive state
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
fragments longer than 10 nucleotides
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
velocity of DNA degradation is four times higher for dsDNA than for ssDNA
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
the 3'-phosphate terminal position contains 78% deoxyadenosine, less than 1% deoxycytidine, 6% deoxyguanosine, 16% thymidine, the 5'-hydroxy position contains 38% thymidine, 24% deoxyguanosine, 21% deoxyadenosine and 17% deoxycytidine
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
double-strand and single-strand DNA, but the enzyme acts preferentially on double-strand DNA
this endonuclease makes double-strand breaks in the DNA substrate
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
double-strand and single-strand DNA, but the enzyme acts preferentially on double-strand DNA
this endonuclease makes double-strand breaks in the DNA substrate
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
native DNA degraded 3-4 times faster than denatured DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
native DNA degraded 5-15 times faster than denatured DNA
average chain length 11 to 15mers
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
preference for double-stranded DNA, terminal four 3'-nucleotides resistant to cleavage
prominent fragments longer than eight nucleotides
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
double-strand DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
ethanol stress on ARPE-19 cells can induce a pathway which is a form of programmed cell death with characteristics of both apoptosis and necrosis, possibly by triggering conversion of leukocyte elastase inhibitor LEI to L-DNase II
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
DNase II exhibits a catalytic domain common to phospholipase D superfamily
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
the enzyme in macrophages appears to be responsible for destroying the nuclear DNA expelled from erythroid precursor cells
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
it is proposed that DNase II together with the Chk2, p53, and p21 pathway forms a genetic barrier blocking the replication of potentially harmful DNA introduced via apoptotic bodies, thereby preventing transformation and malignant development
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
limited digest yields fragments larger than tetranucleotides, exhaustive digestion yields mono-, di- and trinucleotides, where at the 3'-terminus G predominates
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
The LEI/L-DNase II pathway is activated in light-induced retinal degeneration
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
The LEI/L-DNase II pathway is activated in light-induced retinal degeneration
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
native DNA degraded 2.5 times faster than heat-denatured DNA
oligonucleotides with an average chain length of about seven
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA, micrococcus luteus DNA and poly[d(A-T)d(A-T)]
formation of dinucleotides to 8 nucleotide chain length
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
-
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
-
average chain length of oligonucleotides 10-12
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
calf thymus DNA
-
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
double-strand and single-strand scission
formation of oligonucleotides of an average chain length from 14 to 100 in the middle activity phase, purine nucleotides form about 75% of the 3'-terminals, formation of oligonucleotides of an average chain length from 6 to 14 in the terminal activity phase
?
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
independence to double-strand and single-strand break mechanism
-
?
DNA + H2O
?
-
-
-
-
?
DNA + H2O
?
method of ToLFP (topoisomerase ligation fluorescence probes) is used for directly visualizing DNA fragments generated by DNase II in Caenorhabditis elegans embryos. The ratio of non-autonomous and autonomous modes of DNase II is about 3-7. ToLFP method can be used to differentiate the locations of blastomeres where DNase II acts autonomously or non-autonomously in degrading apoptotic DNA
-
-
?
DNA + H2O
?
-
degradation of lambda DNA/HindIII
-
?
DNA + H2O
?
-
the enzyme could be one of the factors in the late block to polyspermy in the cytoplasm of avian eggs, the enzyme activity might also be responsible for poor efficiency in obtaining transgenic birds by microinjection of exogenous DNA into the fertilised chick ovum
-
?
DNA + H2O
?
-
the enzyme acts on both dsDNA and ssDNA, dsDNA is preferred, the enzyme causes double-strand breaks in DNA substrates and generates 3-phosphate and 5-OH termini
-
-
?
DNA + H2O
?
the enzyme possesses a typical divalent iron-independent DNA catalytic activity
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
calf thymus DNA
-
?
DNA + H2O
oligonucleotides + ?
-
E. coli DNA
-
?
DNA + H2O
oligonucleotides + ?
-
induces DNA digestion during apoptosis
-
?
DNA + H2O
oligonucleotides + ?
-
calf thymus DNA
-
?
DNA + H2O
oligonucleotides + ?
-
cleaves DNA in co-ordination with other nucleases to produce internucleosomal degradation
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
salmon sperm DNA
-
?
DNA + H2O
oligonucleotides + ?
-
salmon sperm DNA
-
?
DNA + H2O
oligonucleotides + ?
induces DNA digestion during apoptosis
-
?
DNA + H2O
oligonucleotides + ?
-
directed into the secretory pathway
-
?
DNA + H2O
oligonucleotides + ?
-
directed into the secretory pathway
-
?
DNA + H2O
oligonucleotides + ?
-
involved in nuclear DNA metabolism
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
SV40 minichromosome and naked SV40 DNA, preferentially cleaves the SV40 minichromosome at a distinct 72 bp modulator element, near the replication origin, near the T-ag binding site II and near the BamHI site, where termination of replication and late transcription occurs
-
?
DNA + H2O
oligonucleotides + ?
-
nearest cuts are staggered by 4 nucleotides, 3'end extending, staggered cuts are symmetrically located about a dyad axis in the nucleosome core
-
?
DNA + H2O
oligonucleotides + ?
-
prefers to cut purine-rich steps in duplex-DNA, will also attack heat-denatured and single-stranded substrates
-
?
DNA + H2O
oligonucleotides + ?
-
salmon sperm DNA
-
?
DNA + H2O
oligonucleotides + ?
-
directed into the secretory pathway
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
E. coli DNA
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
-
-
?
DNA + H2O
oligonucleotides + ?
-
genomic DNA
-
?
DNA + H2O
oligonucleotides + ?
-
induces DNA digestion during apoptosis
-
?
DNA + H2O
oligonucleotides + ?
-
involvement in recombination, replication and degradation of DNA
-
?
DNA + H2O
oligonucleotides + ?
-
directed into the secretory pathway
-
?
DNA + H2O
oligonucleotides + ?
-
transformation from leukocyte elastase inhibitor to L-DNase II acts as a switch of protease and nuclease pathways
-
?
nuclei + H2O
oligonucleotides + ?
-
involved in DNA replication
-
?
nuclei + H2O
oligonucleotides + ?
-
convertes genomic DNA into fragments of less than 2 kbp
-
?
nuclei + H2O
oligonucleotides + ?
-
-
-
?
nuclei + H2O
oligonucleotides + ?
-
the 6.5 kbp EcoRI fragment containing the beta-globin gene region shows a 6fold increased sensitivity to DNase II
-
?
plasmid DNA + H2O
?
-
-
-
?
plasmid DNA + H2O
?
-
-
-
-
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
-
IgGs from rabbit immunized with DNase II effectively hydrolyze DNA
-
-
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
-
no sequence specificity
primary reaction product, "nicking" mechanism
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
-
plasmid pBR322
primary reaction product, "nicking" mechanism
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
-
introduces a strong excess of single-strand nicks relative to double-strand breaks, more than 50% converted to nicked circular DNA
-
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
-
plasmid pBR322
primary reaction product, "nicking" mechanism
?
additional information
?
-
The enzyme is required for digesting DNA of apoptotic cell corpses and dietary DNA, it is not required for viability.
-
?
additional information
?
-
The enzyme is required for digesting DNA of apoptotic cell corpses and dietary DNA, it is not required for viability.
-
?
additional information
?
-
The enzyme is required for digesting DNA of apoptotic cell corpses and dietary DNA, it is not required for viability.
-
?
additional information
?
-
-
The enzyme is required for digesting DNA of apoptotic cell corpses and dietary DNA, it is not required for viability.
-
?
additional information
?
-
-
The enzyme plays a role in the degradation of exogenous DNA encountered by phagocytosis and is important for DNA fragmentation and degradation during cell death
-
?
additional information
?
-
-
The enzyme plays a role in the degradation of exogenous DNA encountered by phagocytosis and is important for DNA fragmentation and degradation during cell death
-
?
additional information
?
-
The enzyme is required for digesting DNA of apoptotic cell corpses and dietary DNA, it is not required for viability.
-
?
additional information
?
-
-
enzyme is essential to maintaining proper immune function in Drosophila
-
-
?
additional information
?
-
-
the enzyme is required for degrading DNA of dying cells and this function is necessary for the proper removal of apoptotic nuclei during fetal development
-
?
additional information
?
-
-
enzyme precursor Leukocyte Elastase Inhibitor is cleaved by elastase at its reactive center loop. Cleavage abolishes the antiprotease activity and leads to a conformational modification that exposes an endonuclease active site and a nuclear localization signal. Both endonuclease activity and nuclear translocation are needed to induce cell death
-
-
?
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Baker, K.P.; Baron, W.F.; Henzel, W.J.; Spencer, S.A.
Molecular cloning and characterization of human and murine DNase II
Gene
215
281-289
1998
Homo sapiens, Mus musculus, Sus scrofa
brenda
Krieser, R.J.; Eastman, A.
The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis
J. Biol. Chem.
273
30909-30914
1998
Homo sapiens (O00115), Homo sapiens
brenda
Takeshita, H.; Yasuda, T.; Iida, R.; Nakajima, T.; Hosomi, O.; Nakashima, Y.; Mori, S.; Nomoto, H.; Kishi, K.
Identification of the three non-identical subunits cnstituting human deoxyribonuclease II
FEBS Lett.
440
239-242
1998
Homo sapiens
brenda
Torriglia, A.; Perani, P.; Brossas, J.Y.; Chaudun, E.; Treton, J.; Courtois, Y.; Counis, M.F.
L-DNase II, a molecule that links proteases and endonucleases in apoptosis, derives from the ubiquitous serpin leukocyte elastase inhibitor
Mol. Cell. Biol.
18
3612-3619
1998
Sus scrofa
brenda
Torriglia, A.; Chaudun, E.; Chany-Fournier, F.; Jeanny, J.C.; Courtois, Y.; Counis, M.F.
Involvement of DNase II in nuclear degeneration during lens cell differentiation
J. Biol. Chem.
270
28579-28585
1995
Gallus gallus
brenda
Barry, M.A.; Eastman, A.
Identification of deoxyribonuclease II as an endonuclease involved in apoptosis
Arch. Biochem. Biophys.
300
440-450
1993
Cricetulus griseus
brenda
Yasuda, T.; Nadano, D.; Awazu, S.; Kishi, K.
Human urine deoxyribonuclease II (DNase II) isoenzymes: a novel immunoaffinity purification, biochemical multiplicity, genetic heterogeneity and broad distribution among tissues and body fluids
Biochim. Biophys. Acta
1119
185-193
1992
Homo sapiens
brenda
Harosh, I.; Binninger, D.M.; Harris, P.V.; Mezzina, M.; Boyd, J.B.
Mechanism of action of deoxyribonuclease II from human lymphoblasts
Eur. J. Biochem.
202
479-484
1991
Homo sapiens
brenda
Liao, T.H.; Liao, W.C.; Chang, H.C.; Lu, K.S.
Deoxyribonuclease II purified from the isolated lysosomes of porcine spleen and from porcine liver homogenates. Comparison with deoxyribonuclease II purified from porcine spleen homogenates
Biochim. Biophys. Acta
1007
15-22
1989
Sus scrofa
brenda
Spitzer, S.; Eckstein, F.
Inhibition of deoxyribonucleases by phosphorothioate groups in oligonucleotides
Nucleic Acids Res.
16
11691-11704
1988
Sus scrofa
brenda
Galcheva-Gargova, Z.; Davidov, V.; Dessev, G.
Formation of single-stranded regions in the course of digestion of DNA with DNaseII and micrococcal nuclease
Arch. Biochem. Biophys.
240
464-469
1985
Mammalia
brenda
Drew, H.R.
Structural specificities of five commonly used DNA nucleases
J. Mol. Biol.
176
535-557
1984
Mammalia
brenda
Anai, M.; Muta, A.; Umeno, M.; Sasaki, M.
Purification and properties of an acid deoxyribonuclease from rat small intestinal mucosa
J. Biochem.
94
339-344
1983
Rattus sp.
brenda
Eshima, N.; Muta, A.; Anai, M.
Inhibitory effect of bovine serum albumin on acid deoxyribonuclease from rat small intestinal mucosa
J. Biochem.
94
345-352
1983
Cavia porcellus, Rattus sp.
brenda
Wood, W.I.; Felsenfeld, G.
Chromatin structure of the chicken beta-globin gene region
J. Biol. Chem.
257
7730-7736
1982
Gallus gallus
brenda
Shakhov, A.N.; Nedospasov, S.A.; Georgiev, G.P.
Deoxyribonuclease III as a probe to sequence-specific chromatin organisation: preferential cleavage in the 72 bp modulator sequence of SV40 minichromosome
Nucleic Acids Res.
10
3951-3965
1982
Mammalia
brenda
Keys, D.S.; Zbarsky, S.H.
Isolation of deoxyribonuclease II from bovine intestinal mucosa
Can. J. Biochem.
58
749-753
1980
Bos taurus
brenda
Horz, W.; Miller, F.; Klobeck, G.; Zachau, H.G.
Deoxyribonuclease II as a probe for chromatin structure. II. Mode of cleavage
J. Biol. Chem.
144
328-351
1980
Mammalia
-
brenda
Horz, W.; Zachau, H.G.
Deoxyribonuclease II as a probe for chromatin structure. I. Location of cleavage sites
J. Biol. Chem.
144
305-327
1980
Mammalia
brenda
Murai, K.; Yamanaka, M.; Akagi, K.; Anai, M.
Purification and properties of deoxyribonuclease II from human urine
J. Biochem.
87
1097-1103
1980
Homo sapiens
brenda
Sollner-Webb, B.; Melchior, W.; Felsenfeld jr., G.
DNase I, DNase II and staphylococcal nuclease cut at different, yet symmetrically located, sites in the nucleosome core
Cell
14
611-627
1978
Mammalia
brenda
Sieliwanowicz, B.; Yamamoto, M.; Stasuik, L.; Laskowski sr., M.
Studies of specificity of deoxyribonuclease from salmon testes
Biochemistry
14
39-46
1975
Oncorhynchus tshawytscha
brenda
Slor, H.
Purification of 14C-labelled deoxyribonuclease II from HeLa S3 lysosomes and its use as a marker for the study of nuclear deoxyribonuclease II
Biochem. J.
136
83-87
1973
Homo sapiens
brenda
Laval, J.; Thiery, J.P.; Ehrlich, S.D.; Paoletti, C.; Bernardi, G.
Studies on the specificity of an acid deoxyribonuclease from Helix aspersa (Mll.)
Eur. J. Biochem.
40
133-137
1973
Cornu aspersum
brenda
Schabort, J.C.
Affinity chromatography of bovine pancreatic and hog spleen deoxyribonucleases
J. Chromatogr.
73
253-256
1972
Sus scrofa
brenda
Dulaney, J.T.; Touster, O.
Isolation of deoxyribonuclease II of rat liver lysosomes
J. Biol. Chem.
247
1424-1432
1972
Rattus sp.
brenda
Yamamoto, M.; Bicknell, L.H.
Salmon testes deoxyribonuclease: large-scale purification and properties
Arch. Biochem. Biophys.
151
261-269
1972
Oncorhynchus tshawytscha
-
brenda
Sicard, P.J.; Obrenovich, A.; Sadron, A.
A new approach to the elucidation of the initial kinetics of neutral and acid deoxyribonucleases
Biochim. Biophys. Acta
268
468-479
1972
Sus scrofa
brenda
Bernarde, G.
Spleen acid deoxyribonuclease
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
271-287
1971
Sus scrofa
-
brenda
Slor, H.; Lev, T.
Acid deoxyribonuclease activity in purified calf thymus nuclei
Biochem. J.
123
993-995
1971
Bos taurus
brenda
Maly, A.
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Ovis aries aries
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Homo sapiens
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Bos taurus
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Bos taurus
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1968
Sus scrofa
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Bond-cleaving specificity of a deoxyribonuclease II from mouse mammary tumors
Biochim. Biophys. Acta
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1967
Mus musculus
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Popov, A.P.; Konichev, A.S.; Tsvetkov, I.L.
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Viviparus viviparus
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Homo sapiens (Q8WZ79), Homo sapiens
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Homo sapiens
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Deoxyribonuclease IIalpha is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality
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Mus musculus
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Ikeda, S.; Takata, N.
Deoxyribonuclease II purified from Euglena gracilis SM-ZK, a chloroplast-lacking mutant: comparison with porcine spleen deoxyribonuclease II
Comp. Biochem. Physiol. B
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Euglena gracilis, Sus scrofa, Euglena gracilis SM-ZK
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Chou, S.F.; Chen, H.L.; Lu, S.C.
Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells
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Homo sapiens
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The C. elegans apoptotic nuclease NUC-1 is related in sequence and activity to mammalian DNase II
Gene
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2000
Caenorhabditis elegans
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Krieser, R.J.; MacLea, K.S.; Park, J.P.; Eastman, A.
The cloning, genomic structure, localization, and expression of human deoxyribonuclease IIbeta
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Homo sapiens (Q8WZ79), Homo sapiens, Mus musculus
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MacLea, K.S.; Krieser, R.J.; Eastman, A.
A family history of deoxyribonuclease II: surprises from Trichinella spiralis and Burkholderia pseudomallei
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2003
Homo sapiens (O00115), Homo sapiens (Q8WZ79), Homo sapiens, Sus scrofa (O62855), Caenorhabditis elegans (P34387), Caenorhabditis elegans (P34508), Caenorhabditis elegans (Q17778), Caenorhabditis elegans, Bos taurus (P56541), Mus musculus (P56542), Mus musculus (Q9QY48), Drosophila melanogaster (Q7JYM9), Rattus norvegicus (Q9QZK8), Rattus norvegicus (Q9QZK9), Caenorhabditis elegans F09G8.2 (P34387), Caenorhabditis elegans NUC-1 (Q17778), Caenorhabditis elegans K04H4.6 (P34508)
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Evans, C.J.; Aguilera, R.J.
DNase II: genes, enzymes and function
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Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Caenorhabditis elegans NUC-1
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Wu, Y.C.; Stanfield, G.M.; Horvitz, H.R.
NUC-1, a caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis
Genes Dev.
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Caenorhabditis elegans
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Kawane, K.; Fukuyama, H.; Kondoh, G.; Takeda, J.; Ohsawa, Y.; Uchiyama, Y.; Nagata, S.
Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver
Science
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2001
Mus musculus
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Stepinska, U.; Olszanska, B.
Detection of deoxyribonuclease I and II activities in Japanese quail oocytes
Zygote
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Coturnix japonica
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Cymerman, I.A.; Meiss, G.; Bujnicki, J.M.
DNase II is a member of the phospholipase D superfamily
Bioinformatics
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Homo sapiens
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Overbo, K.; Myrnes, B.
Deoxyribonuclease II from the Icelandic scallop (Chlamys islandica): isolation and partial characterization
Comp. Biochem. Physiol. B
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Chlamys islandica
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Maclea, K.S.; Cheng, H.H.
Cloning and expression of deoxyribonuclease II from chicken
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Gallus gallus (Q2XP49), Gallus gallus
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Ye, M.; Hu, Z.; Fan, Y.; He, L.; Xia, F.; Zou, G.
Purification and characterization of an acid deoxyribonuclease from the cultured mycelia of Cordyceps sinensis
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Ophiocordyceps sinensis
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Shin, H.D.; Park, B.L.; Cheong, H.S.; Lee, H.S.; Jun, J.B.; Bae, S.C.
DNase II polymorphisms associated with risk of renal disorder among systemic lupus erythematosus patients
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Homo sapiens
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Bergsmedh, A.; Ehnfors, J.; Kawane, K.; Motoyama, N.; Nagata, S.; Holmgren, L.
DNase II and the Chk2 DNA damage pathway form a genetic barrier blocking replication of horizontally transferred DNA
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2006
Mus musculus
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Brossas, J.Y.; Tanguy, R.; Brignole-Baudouin, F.; Courtois, Y.; Torriglia, A.; Treton, J.
L-DNase II associated with active process during ethanol induced cell death in ARPE-19
Mol. Vis.
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2004
Homo sapiens
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Chahory, S.; Padron, L.; Courtois, Y.; Torriglia, A.
The LEI/L-DNase II pathway is activated in light-induced retinal degeneration in rats
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2004
Rattus norvegicus, Rattus norvegicus Fischer
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Shiomi, K.; Midorikawa, S.; Ishida, M.; Nagashima, Y.; Nagai, H.
Plancitoxins, lethal factors from the crown-of-thorns starfish Acanthaster planci, are deoxyribonucleases II
Toxicon
44
499-506
2004
Acanthaster planci (Q75WF2)
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Fombonne, J.; Padron, L.; Enjalbert, A.; Krantic, S.; Torriglia, A.
A novel paraptosis pathway involving LEI/L-DNaseII for EGF-induced cell death in somato-lactotrope pituitary cells
Apoptosis
11
367-375
2006
Rattus norvegicus
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Cheng, Y.C.; Hsueh, C.C.; Lu, S.C.; Liao, T.H.
Identification of three crucial histidine residues (His115, His132 and His297) in porcine deoxyribonuclease II
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Sus scrofa (O62855), Sus scrofa
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Seong, C.S.; Varela-Ramirez, A.; Aguilera, R.J.
DNase II deficiency impairs innate immune function in Drosophila
Cell. Immunol.
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Drosophila melanogaster
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Lopez-Oliva, M.E.; Agis-Torres, A.; Marquez, E.; Munoz-Martinez, E.
Growth hormone modulates the degradative capacity of muscle nucleases but not of cathepsin D in post-weaning mice
Comp. Biochem. Physiol. A
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2007
Mus musculus
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brenda
Overbo, K.; Myrnes, B.
Deoxyribonuclease II from the Icelandic scallop (Chlamys islandica): Isolation and partial characterization
Comp. Biochem. Physiol. B
143B
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2006
Chlamys islandica
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Nakahara, M.; Nagasaka, A.; Koike, M.; Uchida, K.; Kawane, K.; Uchiyama, Y.; Nagata, S.
Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens
FEBS J.
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Mus musculus
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Lopez-Oliva, M.E.; Agis-Torres, A.; Munoz-Martinez, E.
The modulator effect of GH on skeletal muscle lysosomal enzymes is dietary protein dependent
Growth Horm. IGF Res.
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2007
Mus musculus
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De Maria, A.; Bassnett, S.
DNase IIbeta distribution and activity in the mouse lens
Invest. Ophthalmol. Vis. Sci.
48
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2007
Mus musculus
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Cherepanova, A.; Tamkovich, S.; Pyshnyi, D.; Kharkova, M.; Vlassov, V.; Laktionov, P.
Immunochemical assay for deoxyribonuclease activity in body fluids
J. Immunol. Methods
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2007
Homo sapiens
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Padron-Barthe, L.; Lepretre, C.; Martin, E.; Counis, M.F.; Torriglia, A.
Conformational modification of serpins transforms leukocyte elastase inhibitor into an endonuclease involved in apoptosis
Mol. Cell. Biol.
27
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2007
Sus scrofa
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Valamanesh, F.; Torriglia, A.; Savoldelli, M.; Gandolphe, C.; Jeanny, J.C.; BenEzra, D.; Behar-Cohen, F.
Glucocorticoids induce retinal toxicity through mechanisms mainly associated with paraptosis
Mol. Vis.
13
1746-1757
2007
Rattus norvegicus
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Schaefer, P.; Cymerman, I.A.; Bujnicki, J.M.; Meiss, G.
Human lysosomal DNase IIalpha contains two requisite PLD-signature (HxK) motifs: evidence for a pseudodimeric structure of the active enzyme species
Protein Sci.
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2007
Homo sapiens
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Fafandel, M.; Bihari, N.; Peric, L.; Cenov, A.
Effect of marine pollutants on the acid DNase activity in the hemocytes and digestive gland of the mussel Mytilus galloprovincialis
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Mytilus galloprovincialis
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Torriglia, A.; Lepretre, C.; Padron-Barthe, L.; Chahory, S.; Martin, E.
Molecular mechanism of L-DNase II activation and function as a molecular switch in apoptosis
Biochem. Pharmacol.
76
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Gallus gallus, Cricetulus griseus, Homo sapiens, Mus musculus, Rattus norvegicus
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Lepretre, C.; Fleurier, Y.; Martin, E.; Torriglia, A.
Nuclear export of LEI/L-DNase II by Crm1 is essential for cell survival
Biochim. Biophys. Acta
1783
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2008
Cricetulus griseus, Homo sapiens, Sus scrofa
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Padron-Barthe, L.; Courta, J.; Lepretre, C.; Nagbou, A.; Torriglia, A.
Leukocyte Elastase Inhibitor, the precursor of L-DNase II, inhibits apoptosis by interfering with caspase-8 activation
Biochim. Biophys. Acta
1783
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2008
Sus scrofa
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Liu, M.F.; Wu, X.P.; Wang, X.L.; Yu, Y.L.; Wang, W.F.; Chen, Q.J.; Boireau, P.; Liu, M.Y.
The functions of Deoxyribonuclease II in immunity and development
DNA Cell Biol.
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Drosophila sp. (in: flies), Haemonchus contortus, Homo sapiens, Mus musculus, Sus scrofa, Trichinella spiralis, Acanthaster planci
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Huang, R.T.; Liao, T.H.; Lu, S.C.
Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation
FEBS J.
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Sus scrofa
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Krasnorutskii, M.A.; Buneva, V.N.; Nevinsky, G.A.
Immunization of rabbits with DNase II leads to formation of polyclonal antibodies with DNase and RNase activities
Int. Immunol.
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349-360
2009
Bos taurus
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Lepretre, C.; Scovassi, A.I.; Shah, G.M.; Torriglia, A.
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis
Int. J. Biochem. Cell Biol.
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Sus scrofa
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Ueki, M.; Takeshita, H.; Fujihara, J.; Kimura-Kataoka, K.; Iida, R.; Yuasa, I.; Nakajima, T.; Kominato, Y.; Yasuda, T.
Genetic and expression analysis of all 7 non-synonymous single nucleotide polymorphisms in the human deoxyribonuclease II gene, with potential relevance to autoimmunity
Clin. Chim. Acta
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2010
Homo sapiens (O00115), Homo sapiens
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Lai, H.; Lo, S.; Kage-Nakadai, E.; Mitani, S.; Xue, D.
The roles and acting mechanism of Caenorhabditis elegans DNase II genes in apoptotic DNA degradation and development
PLoS ONE
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2009
Caenorhabditis elegans, Caenorhabditis elegans N2 Bristol
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Montero, J.A.; Lorda-Diez, C.I.; Certal, A.C.; Moreno, N.; Rodriguez-Leon, J.; Torriglia, A.; Hurle, J.M.
Coordinated and sequential activation of neutral and acidic DNases during interdigital cell death in the embryonic limb
Apoptosis
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2010
Gallus gallus
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Crow, Y.J.
The story of DNase II: a stifled death-wish leads to self-harm
Eur. J. Immunol.
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Mus musculus
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Fischer, H.; Scherz, J.; Szabo, S.; Mildner, M.; Benarafa, C.; Torriglia, A.; Tschachler, E.; Eckhart, L.
DNase 2 is the main DNA-degrading enzyme of the stratum corneum
PLoS ONE
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Homo sapiens, Mus musculus
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Jaadane, I.; Nagbou, A.; Behar-Cohen, F.; Torriglia, A.
Interaction of leukocyte elastase inhibitor/L-DNase II with BCL-2 and BAX
Biochim. Biophys. Acta
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2014
Homo sapiens
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Ohkouchi, S.; Shibata, M.; Sasaki, M.; Koike, M.; Safig, P.; Peters, C.; Nagata, S.; Uchiyama, Y.
Biogenesis and proteolytic processing of lysosomal DNase II
PLoS ONE
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Mus musculus
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Laine, L.M.; Biddau, M.; Byron, O.; Mueller, S.
Biochemical and structural characterization of the apicoplast dihydrolipoamide dehydrogenase of Plasmodium falciparum
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Caenorhabditis elegans (Q17778)
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Yu, H.; Lai, H.J.; Lin, T.W.; Chen, C.S.; Lo, S.J.
Loss of DNase II function in the gonad is associated with a higher expression of antimicrobial genes in Caenorhabditis elegans
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2015
Caenorhabditis elegans (P34387), Caenorhabditis elegans (Q17778), Caenorhabditis elegans
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Hou, N.; Piao, X.; Cai, P.; Wu, C.; Liu, S.; Xiao, Y.; Chen, Q.
A novel Schistosoma japonicum endonuclease homologous to DNase II
BMC Genomics
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126
2015
Schistosoma japonicum (Q5DFP6), Schistosoma japonicum
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Qi, X.; Yue, X.; Han, Y.; Jiang, P.; Yang, F.; Lei, J.J.; Liu, R.D.; Zhang, X.; Wang, Z.Q.; Cui, J.
Characterization of two Trichinella spiralis adult-specific DNase II and their capacity to induce protective immunity
Front. Microbiol.
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2504
2018
Trichinella spiralis (Q32R69), Trichinella spiralis (Q32R75), Trichinella spiralis
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Kovacic, I.; Fafandel, M.; Bihari, N.
Lysosomal deoxyribonuclease II from the mussel Mytilus galloprovincialis Characterization and seasonal activity
Mar. Biol. Res.
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716-724
2015
Mytilus galloprovincialis (A0A3L5TW17)
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Chan, M.P.; Onji, M.; Fukui, R.; Kawane, K.; Shibata, T.; Saitoh, S.; Ohto, U.; Shimizu, T.; Barber, G.N.; Miyake, K.
DNase II-dependent DNA digestion is required for DNA sensing by TLR9
Nat. Commun.
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5853
2015
Mus musculus (P56542)
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Varela-Ramirez, A.; Abendroth, J.; Mejia, A.A.; Phan, I.Q.; Lorimer, D.D.; Edwards, T.E.; Aguilera, R.J.
Structure of acid deoxyribonuclease
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2017
Burkholderia thailandensis (Q2T8B0), Burkholderia thailandensis, Burkholderia thailandensis ATCC 700388 (Q2T8B0)
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