Information on EC 3.1.21.1 - deoxyribonuclease I

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.21.1
-
RECOMMENDED NAME
GeneOntology No.
deoxyribonuclease I
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
CAS REGISTRY NUMBER
COMMENTARY hide
9003-98-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC19606T
-
-
Manually annotated by BRENDA team
strain ATCC19606T
-
-
Manually annotated by BRENDA team
eel
SwissProt
Manually annotated by BRENDA team
multifunctional enzyme
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain N2
-
-
Manually annotated by BRENDA team
strain N2
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
spruce budworm
-
-
Manually annotated by BRENDA team
japanese quail
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
2 active compounds, A and B
-
-
Manually annotated by BRENDA team
cv. Thessalia
-
-
Manually annotated by BRENDA team
cv. Luzerne Euver
-
-
Manually annotated by BRENDA team
C57BL/6 mice
Uniprot
Manually annotated by BRENDA team
4 active compounds, A, B, C, D
-
-
Manually annotated by BRENDA team
sea bream
SwissProt
Manually annotated by BRENDA team
sea urgin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Potyvirus sp.
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain MGAS5005
-
-
Manually annotated by BRENDA team
isolate SX332
-
-
Manually annotated by BRENDA team
i.e. PRV
-
-
Manually annotated by BRENDA team
fungus
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
in uninduced K-562 cells, several sites within HMIP-2 show sensitivity to DNase I above background levels. When cells are induced to differentiate, the region shows a general increase in DNase I sensitivity: 3 sites refer to here as HBS1L-MYB HS1, HS2, and HS3, in particular, show a marked increase in sensitivity compared with background levels. DNase I sensitivity also increases for betaHS2 and betaHS3 controls. HBS1L-MYB HS1, HS2, and HS3 also show stronger sensitivity to DNase I than the betaHS3 control, thereby reaching a threshold level for hypersensitivity. No difference in DNase I sensitivity for NEFM in uninduced and induced K-562 cells. BetaHS2 is not sensitive to DNase I in Jurkat cells. Jurkat cells show similar background levels and generally, a similar DNase I sensitivity profile to induced K562 cells, but with much less sensitivity at HBS1L-MYB HS1, HS2, and HS3. The strongest sensitivity to DNase I in Jurkat cells coincides with the putative promoter region of the alternative HBS1L exon (exon 1a), which shows a low degree of sensitivity in K-562 cells (both uninduced and induced)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(pC)10 + H2O
?
show the reaction diagram
-
-
-
-
-
190mer DNA fragment + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
?
3'AMP + H2O
adenosine + phosphate
show the reaction diagram
-
phosphomonoesterase activity
-
?
calf thymus DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
-
?
chromatin + H2O
?
show the reaction diagram
circular plasmid DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
cosmid DNA + H2O
?
show the reaction diagram
-
-
-
-
?
crab d(A-T) polymer + H2O
5'-hexanucleotides + ?
show the reaction diagram
-
unique polymer of alterating A and T contains about 3% G and C residues integrated into its structure
enriched in C and G, sugar specificity may be limited to the nucleotide following the point of cleavage
?
d(pA)10 + H2O
?
show the reaction diagram
-
-
-
-
-
d(pApCpTpApCpApGpTpCpTpApCpA) + H2O
?
show the reaction diagram
-
-
-
-
-
d(pGpGpCpApCpTpTpApC) + H2O
?
show the reaction diagram
-
-
-
-
-
d(pT)10 + H2O
?
show the reaction diagram
-
-
-
-
-
d(pTpApGpApApGpApTpCpApApA) + H2O
?
show the reaction diagram
-
-
-
-
-
d-ApApTp + H2O
pTp + d-ApA
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
DNA + H2O
5'-phosphodinucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
DNA + H2O
5'-phosphotrinucleotides + ?
show the reaction diagram
double-stranded circular DNA + H2O
?
show the reaction diagram
double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
double-stranded DNA + H2O
?
show the reaction diagram
double-stranded linear DNA + H2O
?
show the reaction diagram
dsDNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
dsDNA + H2O
?
show the reaction diagram
Fc-oligo-SH + H2O
?
show the reaction diagram
-
degradation of a thiolated ferrocenyloligonucleotide, efficiency of DNase I reaction on the electrode is 48, 72, or 73% when treated with 1 microl of 2, 1, or 0.5 micromol ferrocenyloligonucleotide, respectively. DNase I can cleave the oligonucleotide on the gold surface and does not show a nonspecific surface absorption
-
-
?
H2AL2 nucleosome core particle + H2O
?
show the reaction diagram
-
DNase I and hydroxyl radical footprinting as well as micrococcal and exonuclease III digestion show alterations in the structure of the histone variant H2AL2 nucleosome all over the nucleosomal DNA length
-
-
?
heat-denatured DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
HMIP-2 + H2O
?
show the reaction diagram
-
identifies three tissue-specific DNase I hypersensitive sites in the core intergenic interval
-
-
?
linearized plasmid-DNA + H2O
5'-phosphooligonucleotides + H2O
show the reaction diagram
NO2-Ph-pdTp-NO2-Ph + H2O
p-nitrophenol + ?
show the reaction diagram
-
-
-
?
NO2-Ph-pdTp-NO2Ph + H2O
p-nitrophenol + NO2-Ph-pdT-3'-phosphate
show the reaction diagram
-
rapidly hydrolyzed at a single bond
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + phenylphosphoric acid
show the reaction diagram
phage M13 DNA + H2O
?
show the reaction diagram
-
endolytically cleavage generating single base nicks
-
?
plasmid DNA + H2O
?
show the reaction diagram
poly(dA) + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
?
poly(dT) + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
?
pUC18 DNA + H2O
?
show the reaction diagram
-
-
-
?
relaxed circular plasmid DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
RNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
RNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
-
?
RNA + H2O
?
show the reaction diagram
-
low activity
-
?
salmon sperm DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
-
?
salmon sperm DNA + H2O
?
show the reaction diagram
salmon testis DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
single-stranded circular DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
preference for single-stranded regions
-
?
single-stranded DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
-
?
single-stranded DNA + H2O
?
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
ssDNA + H2O
?
show the reaction diagram
-
-
-
?
supercoiled DNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
in presence of Mg2+ and Ca2+ under neutral conditions
production of 3'-OH and 5'-phosphate ends
?
supercoiled plasmid DNA + H2O
linear DNA + ?
show the reaction diagram
supercoiled pUC18 DNA + H2O
?
show the reaction diagram
-
endolytically cleavage generating single base nicks
-
?
Xenopus laevis DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
-
degradation of DNA from supernumerary spermatozoa which enter the ovum durig polyspermic fertilisation in birds
-
?
double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
dsDNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
dsDNA + H2O
?
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
-
Cu2+
-
highest activity at 5 mM, but only 40% activtiy compared to Mg2+
KCl
-
stimulating between 25 and 50 mM, inhibitory above 50 mM
MgCl2
Potyvirus sp.
-
-
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(4-amidinophenyl)-6-indolecarbamidine
-
significantly inhibits DNase I activity
2-mercaptoethanol
2-Nitro-5-thiocyanobenzoic acid
2-nitro-5-thiosulfobenzoic acid
5'-deoxy-GMP
-
competitive, product inhibition
actin
Aflatoxin B2a
-
non-competitive inhibitor
Aflatoxin G2
-
non-competitive inhibitor
Aflatoxin G2a
-
non-competitive inhibitor
Aflatoxin M1
-
non-competitive inhibitor
Aprotinin
inhibits DNase1 as a result of plasmin inhibition
aurintricarboxylic acid
i.e ATA, a general inhibitor of nucleases, weak inhibition
Bile acids
-
inhibit the enzyme in concert with cholesterol sulfate
calf spleen inhibitor protein II
-
molecular weight: 59000 Da, forms an inhibitory uni-uni molecular complex with DNase I, maximum stability at pH 7
-
calf thymus inhibitor protein
-
molecular weight: 49000 Da, maximum stability at pH 6
-
carbodiimide
-
presence of divalent cations slows the rate of inactivation
Cholesterol sulfate
-
from human gastric fluid, the sulfate group and the hydrophobic side chain of cholesterol sulfate are indispensable for the inhibitory effect, irreversible, dependent on bile acids, a ratio of 342:1 of bile acids to cholesterol sulfate is required for complete inhibition
Cu-iodoacetate
-
at 0.1 M iodoacetate and 4 mM Cu2+ 50% inhibition in 16 min
dithiothreitol
-
-
DTT
-
80% inhibition at 1 mM
E2-immunity protein
-
complete inhibition of activity on plasmid DNA
-
G-actin
-
gamma-actin
-
guanidinium hydrochloride
-
over 80% inhibition at 0.5 M
heparin
iodoacetate
K+
-
no inhibition at 50 mM, 50% inhibition at 200 mM
mannitol
methanesulfonylchloride
-
inactivation at pH 5.0
N-bromosuccinimide
Na+
-
no inhibition at 50 mM, 50% inhibition at 200 mM
oligonucleotides
-
competitive inhibition
phosphate
plasmin
directly inhibits recombinant DNase1/3, but does not inactivate recombinant DNase1
-
protein Im9
-
an Escherichia coli protein that binds to E9 DNase in the cytosol to protect the cell
-
RNA
-
enhanced activity by pretreatment with ribonuclease, variety of RNA's including tRNA
Somatostatin
2 enzyme forms: a somatostatin-sensitive and a somatostatin-resistant, controls the enzyme level in the lower gut, in vivo transient down-regulation of gene expression of the sensitive enzyme form
Tris
increasing concentrations of Tris (approximately half activity in the presence of 80 mM Tris) have a greater inhibitory influence on rmDNase1/3 than on rmDNase1 (no inhibitory influence of Tris)
Tris-HCl buffer
Trypsin
-
Urea
-
20% inhibition at 4 M
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
aflatoxin B1
-
effective activator below 0.08 mM
Aflatoxin B2
-
effective activator below 0.08 mM
Aflatoxin M2
-
effective activator below 0.08 mM
Aprotinin
activates DNase1/3-like activity
Bile
-
bile from rat , reconstitution of activity of inhibited DNase I-actin complex
-
bovine serum albumin
-
0.01 mg/reaction volume stimulates about 50% at both pH 6.2 and 7.7
-
dithiothreitol
-
-
heparin
activates DNase1
mannitol
-
induces reduction in height and dry weight in seedlings due to increased enzyme activity in the initial growth stages followed by a decrease in subsequent days
N-ethylmaleimide
-
stimulation about 10-20% at both pH 6.2 and 7.7
okadaic acid
-
10 nM, increases accessibility to DNA in chromatin of carcinoma cells, changes the chromatin supraorganization to a more homgenous and fine chromatin texture as compared to control cells, causes apoptosis after exposure longer than 6 h, no effect on CEM-VLB100 cells
Slx4
-
the Slx4 protein is essential for efficient processing of DNA substrates
-
additional information
-
His44 may play a critical role in substrate DNA binding in the putative secondary active site, and introduction of sulfhydryl groups at Thr14 and Ser43 may facilitate Mn2+-coordination and further contribute to the catalytic activity of DNase I
-
KM VALUE [mM]