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Information on EC 3.1.2.6 - hydroxyacylglutathione hydrolase and Organism(s) Bos taurus
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3.1.2.6 hydroxyacylglutathione hydrolaseIUBMB Comments
Also hydrolyses S-acetoacetylglutathione, but more slowly.
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Word Map
- 3.1.2.6
- methylglyoxal
- gsh
- s-d-lactoylglutathione
-
glyoxalases
- d-lactate
-
glycation
-
glutathione-dependent
- dehydroascorbate
- monodehydroascorbate
- hemithioacetal
-
d-lactic
- mdhar
-
dicarbonyls
- trypanothione
- 2-oxoaldehydes
-
4.4.1.5
- lactoylglutathione
- metallo-beta-lactamase
- drug development
- biotechnology
- pharmacology
- medicine
- agriculture
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
glyoxalase ii, glyoxalase 2, gly ii, glyii, glxii, glx2-2, gloii, hydroxyacylglutathione hydrolase, cgloii, glo ii, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetoacetylglutathione hydrolase
Germ cell specific protein
GLO II
glyoxalase II
hydrolase, acetoacetylglutathione
hydrolase, hydroxyacylglutathione
Round spermatid protein RSP29
S-2-hydroxylacylglutathione hydrolase
acetoacetylglutathione hydrolase
-
-
-
-
Germ cell specific protein
-
-
-
-
GLO II
-
-
-
-
glyoxalase II
-
-
-
-
hydrolase, acetoacetylglutathione
-
-
-
-
hydrolase, hydroxyacylglutathione
-
-
-
-
Round spermatid protein RSP29
-
-
-
-
S-2-hydroxylacylglutathione hydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of thioester
hydrolysis of thioester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-(2-hydroxyacyl)glutathione hydrolase
Also hydrolyses S-acetoacetylglutathione, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY
9025-90-5
-
9025-92-7
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N,S-bisfluorenylmethoxycarbonylglutathione + H2O
9-fluorenylmethoxycarboxylate + glutathione
-
-
-
-
?
S-m-nitrobenzyloxycarbonylglutathione + H2O
m-nitrobenzyloxycarboxylate + glutathione
-
-
-
-
?
S-o-nitrobenzyloxycarbonylglutathione + H2O
o-nitrobenzyloxycarboxylate + glutathione
-
-
-
-
?
S-p-nitrobenzyloxycarbonylglutathione + H2O
p-nitrobenzyloxycarboxylate + glutathione
-
-
-
-
?
additional information
?
-
-
the enzyme catalyzes the rate limiting step of the glyoxalase pathway
-
-
?
S-(2-hydroxyacyl)glutathione + H2O
glutathione + a 2-hydroxycarboxylate anion
-
-
-
?
S-(2-hydroxyacyl)glutathione + H2O
glutathione + a 2-hydroxycarboxylate anion
-
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-(2-hydroxyacyl)glutathione + H2O
glutathione + a 2-hydroxycarboxylate anion
-
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
-
?
additional information
?
-
-
the enzyme catalyzes the rate limiting step of the glyoxalase pathway
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
inhibitory effect of methanol-extracts of some edible plants and some medicinal herbs against the glyoxalase II
-
additional information
-
negatively charged phospholipids, like dioleoyl phosphatidic acid, cardiolipin, and phosphatidylserine, specifically interact with the enzyme, but do not inhibit the mitochondrial isozyme, no effect by neutral phospholipids
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.162
S-(2-hydroxyacyl)glutathione
-
mitochondrial isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid
0.191
S-(2-hydroxyacyl)glutathione
-
mitochondrial isozyme, pH 7.2, 25°C, in presence of cardiolipin
0.255
S-(2-hydroxyacyl)glutathione
-
mitochondrial isozyme, pH 7.2, 25°C, in presence of phosphatidylserine
0.283
S-(2-hydroxyacyl)glutathione
-
mitochondrial isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine
additional information
additional information
-
kinetics in presence of liposomes consisting of different phospholipids
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
at least 4 enzyme forms, contain 10% of the total glyoxalase II activity in the homogenate
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GLO2_BOVIN
308
0
34089
Swiss-Prot
Mitochondrion (Reliability: 2)
M5FMT6_BOVIN
308
0
34089
TrEMBL
Mitochondrion (Reliability: 2)
M5FKE2_BOVIN
193
0
21712
TrEMBL
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bae, C.D.; Park, J.B.; Yang, J.U.; Lee, T.H.
Purification and characterization of glyoxylase II from bovine liver
Korean J. Biochem.
27
99-103
1995
Bos taurus
-
Jerzykowski, T.; Piskorska, D.; Ostrowska, M.
The separation of glyoxalase I and glyoxalase II by paper electrophoresis
J. Chromatogr.
116
225-229
1976
Bos taurus
Seddon, A.P.; Bunni, M.; Douglas, K.T.
Photoaffinity labelling by S-(p-azidophenacyl)-glutathione of glyoxalase II and glutathione S-transferase
Biochem. Biophys. Res. Commun.
95
446-452
1980
Bos taurus
Al-Timari, A.; Douglas, K.T.
Inhibition by glutathione derivatives of bovine liver glyoxalase II (hydroxyacylglutathione hydrolase) as a probe of the N- and S-sites for substrate binding
Biochim. Biophys. Acta
870
219-225
1986
Bos taurus
Uotila, L.
Glutathione thiol esterases
Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York
3
767-804
1989
Bos taurus, Saccharomyces cerevisiae, Escherichia coli, Homo sapiens, Mus musculus, Rattus norvegicus
-
Talesa, V.; Principato, G.B.; Norton, S.J.; Contenti, S.; Mangiabene, C.; Rosi, G.
Isolation of glyoxalase II from bovine liver mitochondria
Biochem. Int.
20
53-58
1990
Bos taurus
Yang, J.U.; Bae, C.D.; Park, J.B.; Lee, T.H.
Studies on inhibition of glyoxalase II by synthetic and natural products
Exp. Mol. Med.
28
25-28
1996
Bos taurus
-
Norton, S.J.; Elia, A.C.; Chyan, M.K.; Gillis, G.; Frenzel, C.; Principato, G.B.
Inhibitors and inhibition studies of mammalian glyoxalase II activity
Biochem. Soc. Trans.
21
545-549
1993
Bos taurus
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