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Information on EC 3.1.2.4 - 3-hydroxyisobutyryl-CoA hydrolase and Organism(s) Rattus norvegicus

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.2 Thioester hydrolases
                3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase
IUBMB Comments
Also hydrolyses 3-hydroxypropanoyl-CoA.
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This record set is specific for:
Rattus norvegicus
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hibch, 3-hydroxyisobutyryl-coa hydrolase, smb20752, ngr_b20860, hib-coa hydrolase, hibyl-coa hydrolase, beta-hydroxyisobutyryl-coa hydrolase, beta-hydroxyisobutyryl-coenzyme a hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxy-isobutyryl CoA hydrolase
-
-
-
-
HIBYL-CoA hydrolase
-
-
hydrolase, 3-hydroxyisobutyryl coenzyme A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
SYSTEMATIC NAME
IUBMB Comments
3-hydroxy-2-methylpropanoyl-CoA hydrolase
Also hydrolyses 3-hydroxypropanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-88-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-methylmalonyl-CoA + H2O
CoA + methylmalonate
show the reaction diagram
-
-
-
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
3-hydroxy-2-methylpropanoate + CoA
show the reaction diagram
-
substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
?
show the reaction diagram
3-hydroxy-2-methylpropanoyl-CoA + H2O
CoA + 3-hydroxy-2-methylpropanoate
show the reaction diagram
3-hydroxypropanoyl-CoA + H2O
CoA + 3-hydroxypropanoate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxy-2-methylpropanoyl-CoA + H2O
3-hydroxy-2-methylpropanoate + CoA
show the reaction diagram
-
substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
?
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetoacetyl-CoA
-
competitive
acetyl-CoA
-
competitive
DL-3-hydroxybutyryl-CoA
-
competitive
DL-methylmalonyl-CoA
-
competitive
isobutyryl-CoA
-
competitive
malonyl-CoA
-
competitive
n-valeryl-CoA
-
competitive
propionyl-CoA
-
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
3-hydroxy-2-methylpropanoyl-CoA
-
-
0.025
3-hydroxypropionyl-CoA
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
150 - 266
3-hydroxy-2-methylpropanoyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
hydrolysis of (S)-methylmalonyl-CoA
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10.5
-
pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
5 - 7
-
pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HIBCH_RAT
385
0
43025
Swiss-Prot
Mitochondrion (Reliability: 1)
A0A8I6A0J8_RAT
404
1
45014
TrEMBL
Mitochondrion (Reliability: 2)
A0A8I5ZL23_RAT
383
0
42741
TrEMBL
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 36000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from rat liver, 7200fold, to homogeneity
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hawes, J.W.; Jaskiewicz, J.; Shimomura, Y.; Huang, B.; Bunting, J.; Harper, E.T.; Harris, R.A.
Primary structure and tissue-specific expression of human beta-hydroxyisobutyryl-coenzyme A hydrolase
J. Biol. Chem.
271
26430-26434
1996
Homo sapiens (Q6NVY1), Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Shimomura, Y.; Murakami, T.; Fujitsuka, N.; Nakai, N.; Sato, Y.; Sugiyama, S.; Shimomura, N.; Irwin, J.; Hawes, J.W.; Harris, R.A.
Purification and partial characterization of 3-hydroxyisobutyryl-coenzyme A hydrolase of rat liver
J. Biol. Chem.
269
14248-14253
1994
Rattus norvegicus
Manually annotated by BRENDA team
Shimomura, Y.; Murakami, T.; Nakai, N.; Huang, B.; Hawes, J.W.; Harris, R.A.
3-Hydroxyisobutyryl-CoA hydrolase
Methods Enzymol.
324
229-240
2000
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team