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Information on EC 3.1.2.4 - 3-hydroxyisobutyryl-CoA hydrolase and Organism(s) Arabidopsis thaliana

for references in articles please use BRENDA:EC3.1.2.4
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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.2 Thioester hydrolases
                3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase
IUBMB Comments
Also hydrolyses 3-hydroxypropanoyl-CoA.
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This record set is specific for:
Arabidopsis thaliana
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hibch, 3-hydroxyisobutyryl-coa hydrolase, smb20752, ngr_b20860, hib-coa hydrolase, hibyl-coa hydrolase, beta-hydroxyisobutyryl-coa hydrolase, beta-hydroxyisobutyryl-coenzyme a hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxy-isobutyryl CoA hydrolase
-
-
-
-
beta-hydroxyisobutyryl-CoA hydrolase
-
-
HIBYL-CoA hydrolase
-
-
hydrolase, 3-hydroxyisobutyryl coenzyme A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-hydroxy-2-methylpropanoyl-CoA hydrolase
Also hydrolyses 3-hydroxypropanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-88-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxy-3-phenylpropionyl-CoA + H2O
3-hydroxy-3-phenylpropionate + CoA
show the reaction diagram
-
-
-
?
cinnamoyl-CoA + H2O
cinnamic acid + CoA
show the reaction diagram
-
-
-
?
p-coumaroyl-CoA + H2O
p-coumaric acid + CoA
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
at 1 mM causes more than 90% inhibition of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0029
cinnamoyl-CoA
at 30°C, in 50 mM Tris/HCl buffer, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
in 50 mm Tris-buffer
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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a role for peroxisomal metabolism in cold stress signaling, and plant tolerance to cold stress and darkness-induced starvation. Is needed for fatty acid beta-oxidation and valine catabolism
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HIBC1_ARATH
378
0
42073
Swiss-Prot
other Location (Reliability: 3)
HIBC2_ARATH
378
0
42256
Swiss-Prot
other Location (Reliability: 4)
HIBC3_ARATH
378
0
42567
Swiss-Prot
other Location (Reliability: 5)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CHY1 cDNA cloned behind the 35S promoter in a binary vector, introduced into homozygous chy1-10 mutant plants by Agrobacterium tumefaciens strain GV3101 transformation
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open-reading frame of the Chy1 gene amplified, cDNA ligated into the vector pEX5-CT/TOPO TA to create a fusion of the open-reading frame with a His tag-coding extension at the C-terminus. Plasmid transferred into Escherichia coli BL21 (DE3) cells for expression
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dong, C.H.; Zolman, B.K.; Bartel, B.; Lee, B.H.; Stevenson, B.; Agarwal, M.; Zhu, J.K.
Disruption of Arabidopsis CHY1 reveals an important role of metabolic status in plant cold stress signaling
Mol. Plant
2
59-72
2009
Arabidopsis thaliana
Manually annotated by BRENDA team
Ibdah, M.; Pichersky, E.
Arabidopsis Chy1 null mutants are deficient in benzoic acid-containing glucosinolates in the seeds
Plant Biol.
11
574-581
2009
Arabidopsis thaliana (Q9LKJ1), Arabidopsis thaliana
Manually annotated by BRENDA team