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Information on EC 3.1.2.12 - S-formylglutathione hydrolase and Organism(s) Homo sapiens

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.2 Thioester hydrolases
                3.1.2.12 S-formylglutathione hydrolase
IUBMB Comments
Also hydrolyses S-acetylglutathione, but more slowly.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
serine esterase, esterase d, fgh, sfgh, phest, s-formylglutathione hydrolase, atsfgh, s-formyl-glutathione hydrolase, atu1476, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deubiquitinating enzyme 12
-
-
-
-
esterase D/S-formylglutathione hydrolase
-
-
hydrolase, S-formylglutathione
-
-
-
-
Ubiquitin thiolesterase 12
-
-
-
-
Ubiquitin-specific processing protease 12
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-formylglutathione hydrolase
Also hydrolyses S-acetylglutathione, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-21-0
-
83380-83-0
the former number 50812-21-0 has been deleted
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
S-acetylglutathione + H2O
glutathione + acetate
show the reaction diagram
S-formylglutathione + H2O
glutathione + formate
show the reaction diagram
additional information
?
-
-
esterase D/S-formylglutathione hydrolase plays a key role in glutathione-dependent detoxification of endogenously formed formaldehyde as well as in hydrolysis of various xenobiotics
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-formylglutathione + H2O
glutathione + formate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
esterase D/S-formylglutathione hydrolase plays a key role in glutathione-dependent detoxification of endogenously formed formaldehyde as well as in hydrolysis of various xenobiotics
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11-([5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl]amino)undecyl methylphosphonofluoridoate
-
-
2,4,6-Trinitrobenzene sulfonate
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
arsenite
-
-
ascorbate
-
-
CaCl2
-
-
Co2+
-
-
CuSO4
-
-
folate
-
-
glutathione
-
-
HgCl2
-
-
iodoacetate
-
-
N-ethylmaleimide
-
-
p-hydroxymercuribenzoate
-
-
sarin
-
strong inhibition at 0.0008 mM
Zn2+
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019
4-methylumbelliferyl acetate
-
-
0.49
4-nitrophenyl acetate
-
-
0.12
S-acetylglutathione
-
-
0.2 - 0.29
S-formylglutathione
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.14
-
-
3390
-
-
38.2
-
-
5800
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 7.1
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activation energy 4.5 cal/mol
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ESTD_HUMAN
282
0
31463
Swiss-Prot
other Location (Reliability: 3)
A0A140VJJ2_HUMAN
282
0
31463
TrEMBL
other Location (Reliability: 3)
U3KQT1_HUMAN
262
0
28995
TrEMBL
other Location (Reliability: 3)
X6RA14_HUMAN
253
0
28226
TrEMBL
other Location (Reliability: 3)
H7BZT7_HUMAN
230
0
25480
TrEMBL
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
2 * 30000, SDS-PAGE
32000
-
2 * 32000, SDS-PAGE
52000
-
gel filtration
53000
-
gel filtration
58200
-
calculation from sedimentation and diffusion coefficients
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 31000, SDS-PAGE
dimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.4
-
unstable at
80981
4.5
-
70 min, 30% loss
80981
4.6 - 8.6
-
stable for 30 min at 20°C
80982
5 - 8
-
-
80987
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
unstable
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilization by 2-mercaptoethanol or dithiothreitol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
overexpression of short-form Klotho increases enzyme mRNA and protein expression
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Uotila, L.
Preparation and assay of glutathione thiol esters. Survey of human liver glutathione thiol esterases
Biochemistry
12
3938-3943
1973
Homo sapiens
Manually annotated by BRENDA team
Uotila, L.; Koivusalo, M.
Purification and properties of S-formylglutathione hydrolase from human liver
J. Biol. Chem.
249
7664-7672
1974
Homo sapiens
Manually annotated by BRENDA team
Uotila, L.
Glutathione thiol esterases of human red blood cells. Fractionation by gel electrophoresis and isoelectric focusing
Biochim. Biophys. Acta
580
277-288
1979
Homo sapiens
Manually annotated by BRENDA team
Uotila, L.; Koivusalo, M.
S-Formylglutathione hydrolase
Methods Enzymol.
77
320-325
1981
Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
Koivusalo, M.; Lapatto, R.; Uotila, L.
Purification and characterization of S-formylglutathione hydrolase from human, rat and fish tissues
Adv. Exp. Med. Biol.
372
427-434
1995
Homo sapiens, Lota maculosa, Rattus norvegicus
Manually annotated by BRENDA team
Tuin, A.W.; Mol, M.A.; van den Berg, R.M.; Fidder, A.; van der Marel, G.A.; Overkleeft, H.S.; Noort, D.
Activity-based protein profiling reveals broad reactivity of the nerve agent sarin
Chem. Res. Toxicol.
22
683-689
2009
Homo sapiens
Manually annotated by BRENDA team
Xu, Y.; Sun, Z.
Regulation of S-formylglutathione hydrolase by the anti-aging gene klotho
Oncotarget
8
88259-88275
2017
Homo sapiens, Mus musculus
Manually annotated by BRENDA team