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Information on EC 3.1.16.1 - spleen exonuclease and Organism(s) Plasmodium falciparum

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Plasmodium falciparum
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The taxonomic range for the selected organisms is: Plasmodium falciparum
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates
Synonyms
fen-1, spleen phosphodiesterase, phosphodiesterase ii, 5'-to-3' exonuclease, spleen exonuclease, trm2p, nucleotide pyrophosphatase/phosphodiesterase 1, beef spleen exonuclease, 3'-nucleotide phosphodiesterase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
flap endonuclease-1
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-54-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
deoxyribonucleoside 3'-phosphate + ?
show the reaction diagram
-
-
-
?
Flap34endo + H2O
?
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
yields the highest activity for FEN-1, activity is stimulated above 0.1 mM, reaching a maximum at ca. 10 mM
Mn2+
yields the highest activity for FEN-1
Ni2+
minimally stimulates activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres
Mg2+
inhibits above 20 mM
NaCl
FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000038 - 0.0000045
Flap34endo
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 9.1
Flap34endo
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FEN1_PLAFA
650
0
73990
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
C-terminal truncation mutant, Western blot analysis
75000
sequence analysis, Western blot analysis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
FEN-1 purified by cation exchange column followed by a calmodulin affinity resin, more than 95% pure, C-terminal truncation mutant purified on Ni-NTA column and by cation exchange column, more than 95% pure
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
FEN-1 cloned into the pCAL-n-FLAG expression vector and expressed in Escherichia coli BLR(DE3)pLysS cells. C-terminal truncation mutant cloned into pET24d(+) and expressed in Escherichia coli BL21(DE3)CodonPlus-RIL
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Casta, L.J.; Buguliskis, J.S.; Matsumoto, Y.; Taraschi, T.F.
Expression and biochemical characterization of the Plasmodium falciparum DNA repair enzyme, flap endonuclease-1 (PfFEN-1)
Mol. Biochem. Parasitol.
157
1-12
2008
Plasmodium falciparum (Q9GZ01), Plasmodium falciparum, Plasmodium falciparum FCR-3/C5 (Q9GZ01), Plasmodium yoelii (Q7RME3), Plasmodium yoelii
Manually annotated by BRENDA team