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Information on EC 3.1.11.1 - exodeoxyribonuclease I and Organism(s) Homo sapiens

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Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
phosphodiesterase, dna polymerase i, exonuclease i, exonuclease 1, exo i, hexo1, polb1, 3'-to-5' exonuclease, sbcb15, drpase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'5' exonuclease
-
-
-
-
DNA deoxyribophosphodiesterase
-
-
-
-
dRPase
-
-
-
-
E. coli exonuclease I
-
-
-
-
Escherichia coli exonuclease I
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-
-
-
Exo I
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-
EXO1b
-
-
ExoI
-
-
exonuclease 1
exonuclease 1b
-
-
exonuclease I
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates
show the reaction diagram
study of interaction between enzyme and mismatch repair proteins MLH1, MLH2 and MLH3. The enzyme binds strongly to MLH1 and forms an immunoprecipitable complex with MLH1/MLH2 in vivo
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-46-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-end-labeled dsDNA + H2O
?
show the reaction diagram
-
-
-
-
?
42mer double stranded DNA containing mismatches + H2O
?
show the reaction diagram
the enzyme has a strong capacity for degrading double stranded DNA
-
-
?
5'-end-labeled dsDNA + H2O
?
show the reaction diagram
-
-
-
-
?
biotinylated single-stranded 56mer polynucleotide + H2O
?
show the reaction diagram
-
3'-fluorescent-labeled substrate attached to streptavidin-coated reaction microspheres via a biotin linker
-
-
?
DNA containing double Holliday junctions + H2O
?
show the reaction diagram
-
-
-
?
double stranded DNA containing mismatches + H2O
?
show the reaction diagram
-
-
-
?
forked and nicked dsDNA containing mismatches + H2O
?
show the reaction diagram
the enzyme preferentially cleaves one nucleotide inwards in a double stranded region of a forked and nicked DNA flap substrates
-
-
?
gapped DNA containing mismatches + H2O
?
show the reaction diagram
-
-
-
?
nicked DNA containing mismatches
?
show the reaction diagram
nicked DNA containing mismatches + H2O
?
show the reaction diagram
single-stranded oligodeoxyribonucleotide + H2O
single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate
show the reaction diagram
-
-
-
?
ssDNA + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA containing double Holliday junctions + H2O
?
show the reaction diagram
-
-
-
?
double stranded DNA containing mismatches + H2O
?
show the reaction diagram
-
-
-
?
gapped DNA containing mismatches + H2O
?
show the reaction diagram
-
-
-
?
nicked DNA containing mismatches
?
show the reaction diagram
-
the enzyme is part of the mismatch repair machinery, MMR, the replication factors PCNA and RFC, and Ku70/80 modulate the directionality of the enzyme-mediated excision in DNA mismatch repair, complex components and reaction process, overview
-
-
?
nicked DNA containing mismatches + H2O
?
show the reaction diagram
single-stranded oligodeoxyribonucleotide + H2O
single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate
show the reaction diagram
-
-
-
?
ssDNA + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
required
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
camptothecin
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on exposure to camptothecin, depletion of EXO1 in CtIP-deficient cells increases the frequency of DNA-PK-dependent radial chromosome formation
endonuclease CtIP
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inhibitory effect of CtIP on EXO1 activity using either a radiolabelled DNA oligonucleotide substrate or a linearized plasmid both containing 3'-overhangs
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14-3-3 isoform eta
-
-
14-3-3 isoform sigma
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-
proliferating cell nuclear antigen
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
kinetics of the enzyme located in streptavidin-coated microspheres with biotinylated DNA substrate and Mg2+ cofactor
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 37
the enzyme has approximately a 2fold increase in enzymatic activity at 25°C and 30°C compared with 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
exonuclease 1 is highly conserved from yeast to human
malfunction
metabolism
exonuclease 1 is a multifunctional 5'->3' exonuclease and a DNA structure-specific DNA endonuclease
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EXD2_HUMAN
621
1
70353
Swiss-Prot
Secretory Pathway (Reliability: 3)
EXO1_HUMAN
846
0
94103
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 120000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
ubiquitination
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallization of recombinant wild-type native and selenomethionine-labeled enzyme, and of recombinant mutant D173A enzyme in complex with metal ions, X-ray diffraction structure determination and analysis at 2.5-3.4 A resolution, modelling
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A153V
the mutation is associated with colorectal cancer and cancer of the small intestine
D173A
E109K
the mutation is associated with colorectal cancer
E589K
the mutation is associated with gastric cancer, lung cancer, hepatocellular carcinoma, melanoma, and glioblastoma
E670G
the mutation is associated with gastric cancer, breast cancer, oral cancer, lung cancer, melanoma, and glioblastoma
F506A
-
substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins
F506A/F507A
F507A
-
substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins
N279S
the mutation is associated with breast cancer and pancreatic cancer
P757L
the mutation is associated with colorectal cancer, pancreatic cancer, gastric cancer, breast cancer, oral cancer, lung cancer, and melanoma
Q154A/Y157A
site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution
Q285A/F288A
site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution
R723G/R723S
the mutation is associated with gastric cancer, breast cancer, oral cancer, and lung cancer
S504A
-
substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins
T439M
the mutation is associated with colorectal cancer
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
the enzyme is inactive in reaction mixture after 10 min at 37°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ultraviolet C radiation, ionizing radiation, cisplatin and methylnitronitrosoguanidine do not affect the stability of the enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in insect Sf9 cells
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expression of hEXO1 in Spodoptera frugiperda Sf9 insect cells using the baculovirus vector system
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into plasmid pcDNA3.1A(-), expressing a COOH-terminal His-Myc-tagged EXO1 protein
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murine NIH3T3 cells are transiently cotransfected with plasmids expressing CFP-PCNA and YFP-hEXO1, expression of wild-type and mutant N-terminal YFP-tagged hEXO1 fusion proteins in Escherichia coli strain BL21(DE3), in vitro transcription translation of hEXO1, plasmid pcDNA3.1A/myc-His(-)A-hEXO1 expressing COOH-terminal his6 and myc epitope tagged hEXO1
stable expression of GFP-tagged EXO1 in U2OS cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schmutte, C.; Sadoff, M.M.; Shim, K.S.; Acharya, S.; Fishel, R.
The interaction of DNA mismatch repair proteins with human exonuclease I
J. Biol. Chem.
276
33011-33018
2001
Homo sapiens
Manually annotated by BRENDA team
Werner, J.H.; Cai, H.; Keller, R.A.; Goodwin, P.M.
Exonuclease I hydrolyzes DNA with a distribution of rates
Biophys. J.
88
1403-1412
2005
Homo sapiens
Manually annotated by BRENDA team
El-Shemerly, M.; Janscak, P.; Hess, D.; Jiricny, J.; Ferrari, S.
Degradation of human exonuclease 1b upon DNA synthesis inhibition
Cancer Res.
65
3604-3609
2005
Homo sapiens
Manually annotated by BRENDA team
Surtees, J.A.; Alani, E.
Replication factors license exonuclease I in mismatch repair
Mol. Cell
15
164-166
2004
Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
El-Shemerly, M.; Hess, D.; Pyakurel, A.K.; Moselhy, S.; Ferrari, S.
ATR-dependent pathways control hEXO1 stability in response to stalled forks
Nucleic Acids Res.
36
511-519
2008
Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team
Dherin, C.; Gueneau, E.; Francin, M.; Nunez, M.; Miron, S.; Liberti, S.E.; Rasmussen, L.J.; Zinn-Justin, S.; Gilquin, B.; Charbonnier, J.B.; Boiteux, S.
Characterization of a highly conserved binding site of Mlh1 required for exonuclease I-dependent mismatch repair
Mol. Cell. Biol.
29
907-918
2009
Saccharomyces cerevisiae, Homo sapiens
Manually annotated by BRENDA team
Vallur, A.C.; Maizels, N.
Activities of human exonuclease 1 that promote cleavage of transcribed immunoglobulin switch regions
Proc. Natl. Acad. Sci. USA
105
16508-16512
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Nimonkar, A.V.; Ozsoy, A.Z.; Genschel, J.; Modrich, P.; Kowalczykowski, S.C.
Human exonuclease 1 and BLM helicase interact to resect DNA and initiate DNA repair
Proc. Natl. Acad. Sci. USA
105
16906-16911
2008
Homo sapiens
Manually annotated by BRENDA team
Orans, J.; McSweeney, E.A.; Iyer, R.R.; Hast, M.A.; Hellinga, H.W.; Modrich, P.; Beese, L.S.
Structures of human exonuclease 1 DNA complexes suggest a unified mechanism for nuclease family
Cell
145
212-223
2011
Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team
Liberti, S.E.; Andersen, S.D.; Wang, J.; May, A.; Miron, S.; Perderiset, M.; Keijzers, G.; Nielsen, F.C.; Charbonnier, J.B.; Bohr, V.A.; Rasmussen, L.J.
Bi-directional routing of DNA mismatch repair protein human exonuclease 1 to replication foci and DNA double strand breaks
DNA Repair
10
73-86
2011
Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team
Eid, W.; Steger, M.; El-Shemerly, M.; Ferretti, L.P.; Pena-Diaz, J.; Koenig, C.; Valtorta, E.; Sartori, A.A.; Ferrari, S.
DNA end resection by CtIP and exonuclease 1 prevents genomic instability
EMBO Rep.
11
962-968
2010
Homo sapiens
Manually annotated by BRENDA team
Vallur, A.C.; Maizels, N.
Complementary roles for exonuclease 1 and Flap endonuclease 1 in maintenance of triplet repeats
J. Biol. Chem.
285
28514-28519
2010
Homo sapiens
Manually annotated by BRENDA team
Vallur, A.C.; Maizels, N.
Distinct activities of exonuclease 1 and flap endonuclease 1 at telomeric g4 DNA
PLoS ONE
5
e8908
2010
Homo sapiens
Manually annotated by BRENDA team
Sertic, S.; Pizzi, S.; Cloney, R.; Lehmann, A.R.; Marini, F.; Plevani, P.; Muzi-Falconi, M.
Human exonuclease 1 connects nucleotide excision repair (NER) processing with checkpoint activation in response to UV irradiation
Proc. Natl. Acad. Sci. USA
108
13647-13652
2011
Homo sapiens
Manually annotated by BRENDA team
Keijzers, G.; Bohr, V.A.; Rasmussen, L.J.
Human exonuclease 1 (EXO1) activity characterization and its function on flap structures
Biosci. Rep.
35
e00206
2015
Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team
Keijzers, G.; Liu, D.; Rasmussen, L.
Exonuclease 1 and its versatile roles in DNA repair
Crit. Rev. Biochem. Mol. Biol.
51
440-451
2016
Homo sapiens (Q9UQ84)
-
Manually annotated by BRENDA team
Goellner, E.M.; Putnam, C.D.; Kolodner, R.D.
Exonuclease 1-dependent and independent mismatch repair
DNA Repair
32
24-32
2015
Saccharomyces cerevisiae (P39875), Saccharomyces cerevisiae, Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team
Keijzers, G.; Bakula, D.; Petr, M.A.; Madsen, N.G.K.; Teklu, A.; Mkrtchyan, G.; Osborne, B.; Scheibye-Knudsen, M.
Human exonuclease 1 (EXO1) regulatory functions in DNA replication with putative roles in cancer
Int. J. Mol. Sci.
20
E74
2018
Homo sapiens (Q9UQ84), Homo sapiens
Manually annotated by BRENDA team