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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a, lysophospholipase, neuropathy target esterase, phospholipase b, lysopld, galectin-10, charcot-leyden crystal protein, lysophospholipase a, lysopl, cpla2gamma,
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Charcot-Leyden crystal protein
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Charcot-Leyden crystal protein homolog
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Lysolecithin acylhydrolase
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lysophopholipase L2
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lysophosphatidase
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lysophosphatidylcholine hydrolase
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lysophospholipase A1
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hydrolysis of carboxylic ester
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2-lysophosphatidylcholine acylhydrolase
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1,2-palmitylphosphatidylcholine + H2O
1-palmityl-lysophosphatidylcholine + palmitic acid
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low activity after activating of ExoU with an unknown eukaryotic cell factor
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1-hexyl-glycerophosphocholine + H2O
hexanoate + glycerophosphocholine
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1-lauryl-glycerophosphocholine + H2O
laurate + glycerophosphocholine
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1-oleoyl-glycerophosphocholine + H2O
oleate + glycerophosphocholine
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1-palmitoyl-glycerophosphocholine + H2O
palmitate + glycerophosphocholine
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1-palmitoyl-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
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1-stearoyl-glycerophosphocholine + H2O
stearate + glycerophosphocholine
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2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
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2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
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additional information
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Ca2+ independent phospholipase A2 activity
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arachidonyl trifluoromethyl ketone
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0.025 mM, approx. 50% inhibition
bromoenol lactone
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0.025 mM, approx. 30% inhibition
trifluoromethyl ketone
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0.025 mM, approx. 40% inhibition
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glycerol
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50% increase of lysophospholipase activity in the presence of 30% glycerol
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7.22
1-hexyl-glycerophosphocholine
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at pH 8.0 and 37°C
1.78
1-lauryl-glycerophosphocholine
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at pH 8.0 and 37°C
2.04
1-palmitoyl-glycerophosphocholine
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at pH 8.0 and 37°C
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1016
1-hexyl-glycerophosphocholine
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at pH 8.0 and 37°C
1975
1-lauryl-glycerophosphocholine
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at pH 8.0 and 37°C
921.1
1-palmitoyl-glycerophosphocholine
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at pH 8.0 and 37°C
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110
1-hexyl-glycerophosphocholine
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at pH 8.0 and 37°C
1100
1-lauryl-glycerophosphocholine
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at pH 8.0 and 37°C
450
1-palmitoyl-glycerophosphocholine
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at pH 8.0 and 37°C
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brenda
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brenda
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metabolism
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the enzyme exerts important functions in the homeostasis of phospholipids or detoxification of lysophospholipids
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20000
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x * 20000, SDS-PAGE
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sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM sodium-citrate buffer, pH 4.5
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D17S/L162R
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the mutant shows 2.2fold increased thioesterase activity compared to the wild type
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Ni-NTA column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
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Tamura, M.; Ajayi, T.; Allmond, L.R.; Moriyama, K.; Wiener-Kronish, J.P.; Sawa, T.
Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU
Biochem. Biophys. Res. Commun.
316
323-331
2004
Pseudomonas aeruginosa
brenda
Kovacic, F.; Granzin, J.; Wilhelm, S.; Kojic-Prodic, B.; Batra-Safferling, R.; Jaeger, K.E.
Structural and functional characterisation of TesA - a novel lysophospholipase A from Pseudomonas aeruginosa
PLoS ONE
8
e69125
2013
Pseudomonas aeruginosa
brenda
3.1.1.5 lysophospholipase
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