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Information on EC 3.1.1.5 - lysophospholipase and Organism(s) Homo sapiens

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.5 lysophospholipase
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a, lysophospholipase, neuropathy target esterase, phospholipase b, lysopld, galectin-10, charcot-leyden crystal protein, lysophospholipase a, lysopl, cpla2gamma, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acyl-protein thioesterase 1
-
exhibits deacylation activity as well as lysophospholipase activity in vitro
acylprotein thioesterase 1
-
APT1
-
APT1 exhibits deacylation activity as well as lysophospholipase activity in vitro
ASNase1
-
N-terminal domain
Charcot-Leyden crystal protein
-
-
-
-
Charcot-Leyden crystal protein homolog
-
-
-
-
CLC
-
-
-
-
cPLA2gamma
-
enzyme also possesses transacylation activity: with addition of lysophosphatidic acid/phosphatidic acid, pH above 8, and elevated temperature markedly increases transacylation activity
endothelial lipase
-
-
eosinophil Charcot-Leyden crystal protein
-
-
eosinophil lysophospholipases
-
Galactin-10
-
-
-
-
Galectin-10
-
-
-
-
hLysoPLA I
-
-
lecithinase B
-
-
-
-
lecitholipase
-
-
-
-
Lysolecithin acylhydrolase
-
-
-
-
lysolecithinase
-
-
-
-
lysophopholipase L2
-
-
-
-
lysophosphatidase
-
-
-
-
lysophosphatidylcholine hydrolase
-
-
-
-
lysophospholipase
-
lysophospholipase 1
-
lysophospholipase A1
-
-
-
-
lysophospholipase I
-
isoform
lysophospholipase II
-
isoform
phosphatidase B
-
-
-
-
phospholipase B
phospholipase B precursor
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
2-lysophosphatidylcholine acylhydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-85-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-didecanoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
C10:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C12:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C14:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:1-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:2-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C8:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
myristoyllysophosphatidylcholine is hydrolyzed 50% better than the longer chain palmitoyllysophosphatidylcholine
-
-
?
lysophosphatidylethanolamine + H2O
?
show the reaction diagram
-
-
-
-
?
palmitoyl-Gialpha1 + H2O
palmitic acid + Gialpha1
show the reaction diagram
-
thioesterase activity
-
?
palmitoyl-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
palmitoyl-peptide + H2O
palmitate + peptide
show the reaction diagram
residue 3 to 14 of RGS4 protein with palmitoyl group attached at Cys12 of RGS4 protein
-
-
?
palmitoyl-SCoA + H2O
HS-CoA + palmitic acid
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
additional information
?
-
combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-palmitoyl lysophosphatidylcholine
competitive inhibitor for acylprotein thioesterase activity
DNA containing thymine glycol
-
-
-
lysophosphatidic acid
-
inhibition in a dose-dependent manner
methyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14- tetraenylphosphonofluoridate
-
-
palmitoyl-peptide
competitive inhibitor for lysophospholipase activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
1,2-didecanoylphosphatidylcholine
-
0.0273
1-palmitoyl lysophosphatidylcholine
-
0.00349
palmitoyl-peptide
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0222
1-palmitoyl lysophosphatidylcholine
for acylprotein thioesterase activity
0.0323
palmitoyl-peptide
for lysophospholipase activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.62
substrate 1-palmitoyl lysophosphatidylcholine
2.448
-
-
27.3
substrate palmitoyl-peptide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.3
-
pH 5.0: about 30% of maximal activity, pH 9.3: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
40
-
lysophospholipase reaction proceeds even at 0°C and its rate increases with temperature up to roughly 50°C, the optimal temperature is near 40 °C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
hLysoPLA I expression is induced by phorbol 12-myristate 13-acetate
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
fetal
Manually annotated by BRENDA team
-
enzyme is also located in mitochondria even in the absence of prenylation
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PA24A_HUMAN
749
0
85239
Swiss-Prot
other Location (Reliability: 2)
PA24B_HUMAN
781
0
87978
Swiss-Prot
other Location (Reliability: 5)
PA24C_HUMAN
541
0
60939
Swiss-Prot
other Location (Reliability: 2)
PLB1_HUMAN
1458
1
163081
Swiss-Prot
Secretory Pathway (Reliability: 1)
LIPC_HUMAN
499
0
55914
Swiss-Prot
Secretory Pathway (Reliability: 1)
LPP60_HUMAN
573
0
60883
Swiss-Prot
Mitochondrion (Reliability: 5)
PAG15_HUMAN
412
0
46658
Swiss-Prot
Secretory Pathway (Reliability: 1)
PLPL6_HUMAN
1375
1
150954
Swiss-Prot
other Location (Reliability: 3)
PLPL7_HUMAN
1317
1
145705
Swiss-Prot
Secretory Pathway (Reliability: 5)
PLPL8_HUMAN
782
0
88477
Swiss-Prot
other Location (Reliability: 4)
PLPL9_HUMAN
806
0
89903
Swiss-Prot
Mitochondrion (Reliability: 4)
H7BYX7_HUMAN
1446
1
161636
TrEMBL
Secretory Pathway (Reliability: 1)
B2RWP8_HUMAN
1458
1
162978
TrEMBL
Secretory Pathway (Reliability: 1)
B4DDT1_HUMAN
438
0
48965
TrEMBL
Secretory Pathway (Reliability: 1)
H7C012_HUMAN
510
0
56641
TrEMBL
Secretory Pathway (Reliability: 4)
A8K1Z7_HUMAN
498
0
55766
TrEMBL
Secretory Pathway (Reliability: 1)
A6H8L5_HUMAN
499
0
55556
TrEMBL
Secretory Pathway (Reliability: 1)
E7EUJ1_HUMAN
499
0
55617
TrEMBL
Secretory Pathway (Reliability: 1)
E7EUK6_HUMAN
438
0
49026
TrEMBL
Secretory Pathway (Reliability: 1)
LEG10_HUMAN
142
0
16453
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q6IAQ1_HUMAN
230
0
24670
TrEMBL
-
PLBL1_HUMAN
553
0
63255
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
determined by gel filtration
25000
x * 25000, SDS-PAGE (52000 for GST-fusion protein)
42000
determined by SDS-PAGE and Western Blot analysis
60000
-
x * 60000, SDS-PAGE
61000
-
SDS-PAGE, Flag-tagged fusion protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S119A
part of the catalytic triad, no activity
additional information
-
C-terminal Flag-tag is inserted into the prenylation site. Mutant enzyme shows lysophospholipase activity indicating that C-terminal processing is not essential for activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 50 mM Tris/HCl, pH 7.4, more than one year, stable
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GST affinity chromatography, GST-tag cleaved from GST-fusion protein
using membranes and an anti-Flag column
-
using Sephadex G-75, Mono-S cation-exchange and hydroxyapatite columns
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as an N-terminal myc-tagged and C-terminal Flag-tagged fusion protein in HeLa or HEK293 cells. C-terminal Flag-tag is inserted into the prenylation site
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in HL-60 cells
-
GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lipopolysaccharide suppresses APT1 mRNA and protein expression
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
the phospholipases and lysophospholipases expressed by eosinophils or other airway cells may represent therapeutic targets for blocking surfactant degradation, dysfunction and peripheral airway closure in asthma
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jarvis, A.A.; Cain, C.; Dennis, E.A.
Purification and characterization of a lysophospholipase from human amniotic membranes
J. Biol. Chem.
259
15188-15195
1984
Homo sapiens
Manually annotated by BRENDA team
Wang, A.; Hsiu-Chiung, Y.; Friedman, P.; Johnson, C.A.; Dennis, E.A.
A specific human lysophospholipase: cDNA cloning, tissue distribution and kinetic characterization
Biochim. Biophys. Acta
1437
157-169
1999
Homo sapiens
Manually annotated by BRENDA team
Ackerman, S.J.; Corrette, S.E.; Rosenberg, H.F.; Bennett, J.C.; Mastrianni, D.M.; Nicholson-Weller, A.; Weller, P.F.; Chin, D.T.; Tenen, D.G.
Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase)
J. Immunol.
150
456-468
1993
Homo sapiens
Manually annotated by BRENDA team
Wang, A.; Johnson, C.A.; Jones, Y.; Ellisman, M.H.; Dennis, E.A.
Subcellular localization and PKC-dependent regulation of the human lysophospholipase A/acyl-protein thioesterase in WISH cells
Biochim. Biophys. Acta
1484
207-214
2000
Homo sapiens
Manually annotated by BRENDA team
Gauster, M.; Rechberger, G.; Sovic, A.; Horl, G.; Steyrer, E.; Sattler, W.; Frank, S.
Endothelial lipase releases saturated and unsaturated fatty acids of high density lipoprotein phosphatidylcholine
J. Lipid Res.
46
1517-1525
2005
Homo sapiens
Manually annotated by BRENDA team
Kwatia, M.A.; Doyle, C.B.; Cho, W.; Enhorning, G.; Ackerman, S.J.
Combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
J. Allergy Clin. Immunol.
119
838-847
2007
Mus musculus, Homo sapiens (Q05315)
Manually annotated by BRENDA team
Xu, S.; Zhao, L.; Larsson, A.; Venge, P.
The identification of a phospholipase B precursor in human neutrophils
FEBS J.
276
175-186
2009
Homo sapiens (Q6P4A8), Homo sapiens
Manually annotated by BRENDA team
Yamashita, A.; Tanaka, K.; Kamata, R.; Kumazawa, T.; Suzuki, N.; Koga, H.; Waku, K.; Sugiura, T.
Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)
Biochim. Biophys. Acta
1791
1011-1022
2009
Homo sapiens
Manually annotated by BRENDA team
Hirano, T.; Kishi, M.; Sugimoto, H.; Taguchi, R.; Obinata, H.; Ohshima, N.; Tatei, K.; Izumi, T.
Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1
Biochim. Biophys. Acta
1791
797-805
2009
Homo sapiens (Q6IAQ1), Homo sapiens
Manually annotated by BRENDA team
Satou, M.; Nishi, Y.; Yoh, J.; Hattori, Y.; Sugimoto, H.
Identification and characterization of acyl-protein thioesterase 1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium
Endocrinology
151
4765-4775
2010
Bos taurus, Homo sapiens, Rattus norvegicus, Mus musculus (Q9WTL7)
Manually annotated by BRENDA team
Karamitros, C.S.; Konrad, M.
Human 60-kDa lysophospholipase contains an N-terminal L-asparaginase domain that is allosterically regulated by L-asparagine
J. Biol. Chem.
289
12962-12975
2014
Homo sapiens
Manually annotated by BRENDA team