Information on EC 3.1.1.5 - lysophospholipase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
3.1.1.5
-
RECOMMENDED NAME
GeneOntology No.
lysophospholipase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
hydrolysis of carboxylic ester
Q9U8F2, -
-
hydrolysis of carboxylic ester
Q6P4A8
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
Q8TZI9
-
hydrolysis of carboxylic ester
B0FLU6
-
PATHWAY
KEGG Link
MetaCyc Link
Glycerophospholipid metabolism
-
phosphatidylcholine resynthesis via glycerophosphocholine
-
phospholipid remodeling (phosphatidylethanolamine, yeast)
-
SYSTEMATIC NAME
IUBMB Comments
2-lysophosphatidylcholine acylhydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acyl-protein thioesterase 1
-
exhibits deacylation activity as well as lysophospholipase activity in vitro
acylprotein thioesterase 1
Q6IAQ1
-
AfPlb1
B0Y665
-
AfPlb2
Q9P8P4
-
AfPlb3
B0XZV8
-
APT1
-
APT1 exhibits deacylation activity as well as lysophospholipase activity in vitro
CbPlb1
Q96WX0
-
Charcot-Leyden crystal protein
-
-
-
-
Charcot-Leyden crystal protein homolog
-
-
-
-
CLC
-
-
-
-
CnLYSO1
Q8NKD2
-
cPLA2gamma
-
enzyme also possesses transacylation activity: with addition of lysophosphatidic acid/phosphatidic acid, pH above 8, and elevated temperature markedly increases transacylation activity
endothelial lipase
-
-
eosinophil Charcot-Leyden crystal protein
-
-
eosinophil lysophospholipases
Q05315
-
eosinophil lysophospholipases
-
-
Galactin-10
-
-
-
-
Galectin-10
-
-
-
-
h-LPTA
-
-
hLysoPLA
Q05315
-
hLysoPLA
-
-
hLysoPLA I
-
-
LCAT-like lysophospholipase
-
-
lecithinase B
-
-
-
-
lecitholipase
-
-
-
-
LLPL
-
-
LPLase
Q05315
-
LPLase
-
-
Lysolecithin acylhydrolase
-
-
-
-
lysolecithinase
-
-
-
-
lysophopholipase L2
-
-
-
-
lysophosphatidase
-
-
-
-
lysophosphatidylcholine hydrolase
-
-
-
-
lysophosphatidylcholine lysophospholipase
-
-
lysophosphatidylcholine/transacylase
-
-
lysophospholipase
Q6P4A8
-
lysophospholipase
-
-
lysophospholipase
-
-
lysophospholipase
-
-
lysophospholipase
Q8TZI9
-
lysophospholipase
-
-
lysophospholipase
Q9U8F2
-
lysophospholipase 1
Q6IAQ1
-
lysophospholipase 2
-
-
lysophospholipase 3
-
-
lysophospholipase A
-
-
lysophospholipase A
Q8GNM7
-
lysophospholipase A
Legionella pneumophila JR32
-
-
-
lysophospholipase A1
-
-
-
-
lysophospholipase C
-
-
lysophospholipase I
-
isoform
lysophospholipase I
-
-
lysophospholipase I
-
isoform
lysophospholipase II
-
isoform
lysophospholipase L2
-
-
lysophospholipase-transacylase
-
-
lysoPLA
-
-
lysoPLA
-
-
LysoPLA I
-
-
neuropathy target esterase
B0FLU6
-
NTE
B0FLU6
-
NTE-related esterase
-
-
phosphatidase B
-
-
-
-
phospholipase A
-
-
phospholipase B
-
-
-
-
phospholipase B
-
-
phospholipase B
-
enzymes harbors three distinct activities: a sn-1 and sn-2 fatty acid ester hydrolase, a lysophospholipase and a transacylase activity
phospholipase B
Q96WX0
enzymes harbors three distinct activities: a sn-1 and sn-2 fatty acid ester hydrolase, a lysophospholipase and a transacylase activity
phospholipase B
-
-
phospholipase B
-
enzymes harbors three distinct activities: a sn-1 and sn-2 fatty acid ester hydrolase, a lysophospholipase and a transacylase activity
phospholipase B
Q9P8L1
-
phospholipase B
Q6P4A8
-
phospholipase B
Q939K7
-
phospholipase B
-
enzymes harbors three distinct activities: a sn-1 and sn-2 fatty acid ester hydrolase, a lysophospholipase and a transacylase activity
phospholipase B precursor
Q6P4A8
-
phospholipase B/lipase
-
-
phospholipase B1
-
-
PLB
Q6P4A8
-
PLB
Q939K7
-
PLB/LIP
-
-
PLB1 enzyme
-
-
TAP
-
multifunctional enzyme with thioesterase, esterase, arylesterase, protease and lysophosphilipase activities
TAP
-
multifunctional lysophospholipasethioesterase I/protease I/lysophospholipase L1
thioesterase I/protease I/lysophospholipase L1
-
multifunctional enzyme with thioesterase, esterase, arylesterase, protease and lysophosphilipase activities
thioesterase I/protease I/lysophospholipase L1
-
multifunctional lysophospholipase
lysoPLC
-
enzyme also possesses GPC phoshocholine phosphodiesterase activity and uses glycerophosphorylcholine as a substrate
additional information
-
lysophospholipase activity of lysophosphatidylcholine/transacylase is smaller than the transacylase activity
CAS REGISTRY NUMBER
COMMENTARY
9001-85-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ecotype Col-0
-
-
Manually annotated by BRENDA team
lysophospholipase I and lysophospholipase II
-
-
Manually annotated by BRENDA team
; the genome encodes five phospholipase B genes
-
-
Manually annotated by BRENDA team
precursor; serotype A H99
Q9P8L1
SwissProt
Manually annotated by BRENDA team
var. grubii
GenBank
Manually annotated by BRENDA team
var. grubii H99 wild-type strain and its PLB1 deletion mutant strain (the _plb1 strain)
-
-
Manually annotated by BRENDA team
var. grubii, strain H99
-
-
Manually annotated by BRENDA team
adult Beijing white laying hen
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
var. Advance, at least 3 enzymatic forms: PLP I, PLP II and PLP II'
-
-
Manually annotated by BRENDA team
-
Q8GNM7
SwissProt
Manually annotated by BRENDA team
Legionella pneumophila JR32
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
lysophospholipase I and II
-
-
Manually annotated by BRENDA team
membrane-associated precursor
SwissProt
Manually annotated by BRENDA team
native enzyme and modified enzyme that is generated by the introduction of some nicks into the native type of enzyme
-
-
Manually annotated by BRENDA team
-
Q8TZI9
UniProt
Manually annotated by BRENDA team
2 enzyme forms: phospholipase B1 and phospholipase B2
-
-
Manually annotated by BRENDA team
the genome encodes three phospholipase B genes
-
-
Manually annotated by BRENDA team
; enzyme exhibits the GXSXG consensus active site characteristic of serine proteases, esterases, and lipases, and includes the catalytic triad motif of SerAspHis residues characteristic of serine hydrolases. Three-dimensional structural predictions indicate that the putative catalytic triad formed by these three residues is located at the alpha/beta-hydrolase fold characteristic of the lipases and esterases
UniProt
Manually annotated by BRENDA team
enzyme exhibits the GXSXG consensus active site characteristic of serine proteases, esterases, and lipases, and includes the catalytic triad motif of SerAspHis residues characteristic of serine hydrolases. Three-dimensional structural predictions indicate that the putative catalytic triad formed by these three residues is located at the alpha/beta-hydrolase fold characteristic of the lipases and esterases
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
lysoPL2 knockout mutants show enhanced sensitivity to Zn2+ and H2O2 in comparison to wild type
physiological function
-
LipC plays an important role in the degradation of the outer spore membrane during sporulation
physiological function
-
phospholipase B activity is involved in the process of adhesion and internalization of yeast cells at the MH-S cell surface and may enhance virulence and subsequent down-regulation of macrophage activation
physiological function
-
the serum activity of APT1 plays an important role in determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation
physiological function
-
lysophospholipase 2 can effectively remove lysophosphatidylcholine and detoxify the peroxidized membrane. Lysophospholipase 2-overexpressing Arabidopsis is more tolerant to H2O2 and Cd2+ than wild type, suggesting involvement lysophospholipase-2 in phospholipid repair following lipid peroxidation arising from metal-induced stress. The enzyme confers protection during oxidative stress
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,3-trioleoyl-sn-glycerol + H2O
?
show the reaction diagram
-
-
-
-
?
1,2,3-trioleoyl-sn-glycerol + H2O
?
show the reaction diagram
-
weak
-
-
?
1,2-didecanoylphosphatidylcholine + H2O
?
show the reaction diagram
Q6P4A8
-
-
-
?
1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
D2T083, -
-
-
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dioleoylphosphatidylcholine + H2O
monooleoyl-lysophosphatidylcholine + oleic acid
show the reaction diagram
-
129% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
1,2-dioleoylphosphatidylethanolamine + H2O
monooleoyl-lysophosphatidylethanolamine + oleic acid
show the reaction diagram
-
-
33.5% of activity with 1,2-dipalmitoylphosphatidylcholine
?
1,2-dioleoylphosphatidylserine + H2O
monooleoyl-lysophosphatidylserine + oleic acid
show the reaction diagram
-
-
22.5% of activity with 1,2-dipalmitoylphosphatidylcholine
?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + palmitate
show the reaction diagram
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
-
1,2-dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
Q6P4A8
-
-
-
?
1,2-palmitylphosphatidylcholine + H2O
1-palmityl-lysophosphatidylcholine + palmitic acid
show the reaction diagram
-
low activity after activating of ExoU with an unknown eukaryotic cell factor
-
?
1-acyl-2-lysophosphatidycholine + H2O
fatty acid + glycerophosphocholine
show the reaction diagram
Q9U8F2, -
-
-
-
?
1-acyl-glycerophosphoglycerol + H2O
fatty acid + glcyerophosphoglycerol
show the reaction diagram
-
-
-
-
?
1-acyl-lysophosphatidylcholine + 1-alkyllysophosphatidylcholine
1-alkyl-2-acyl-phosphatidylcholine + glycerylphosphocholine
show the reaction diagram
-
-
-
?
1-acyl-sn-glycero-3-phosphate + H2O
fatty acid + glyceryl phosphate
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phospho-myo-inositol
fatty acid + glycero-3-phospho-myo-inositol
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phospho-myo-inositol
fatty acid + glycero-3-phospho-myo-inositol
show the reaction diagram
-
weak
-
-
?
1-acyl-sn-glycero-3-phosphocholine + H2O
fatty acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phosphocholine + H2O
fatty acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phosphocholine + H2O
fatty acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
1-acyl-sn-glycero-3-phosphocholine + H2O
fatty acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phosphoserine + H2O
fatty acid + glycerophosphoserine
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phosphoserine + H2O
fatty acid + glycerophosphoserine
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phosphoserine + H2O
fatty acid + glycerophosphoserine
show the reaction diagram
-
-
-
-
?
1-acylglycerophosphoethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-acylglycerophosphoethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-arachidonoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-docosahexaenoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-dodecanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
D2T083, -
-
-
-
?
1-hexanoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + hexanoate
show the reaction diagram
-
-
-
-
?
1-hexanoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-lauroyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-lauroyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-lauroyl-sn-glycero-3-phosphocholine + H2O
lauric acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-linoleoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-linoleoyl-sn-glycero-3-phosphocholine + H2O
linoleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-monopalmitoylglycerol + H2O
glycerol + palmitic acid
show the reaction diagram
-
-
lipase activity of LPLA
?
1-monopalmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
-, Q8GNM7
-
-
?
1-myristoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-myristoyl-sn-glycero-3-phosphocholine + H2O
myristic acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-octanoyl-sn-glycero-3-phosphocholine + H2O
octanoic acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-oleoyl lysophosphatidylcholine + H2O
glycerophosphocholine + oleate
show the reaction diagram
-
30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
-
-
?
1-oleoyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
glycerophosphorylcholine + oleic acid
show the reaction diagram
-
30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + oleate
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl-lysophosphatidylcholine + H2O
glycerophosphorylcholine + oleic acid
show the reaction diagram
-
194% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
1-oleoyl-sn-glycero-3-phosphocholine + H2O
oleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphocholine + H2O
oleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphocholine + H2O
oleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
weak
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
Q6IAQ1
-
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-
preferred substrate, LPL1
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-lyso-sn-phosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
-
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
Q6P4A8
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
palmitate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
lysophosphatidylcholine + ?
show the reaction diagram
-
36% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-lysophosphatidylethanolamine + H2O
glycerophosphatidylethanolamine + palmitic acid
show the reaction diagram
-
29% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
1-palmitoyl-lysophosphatidylethanolamine + H2O
glycerophosphatidylethanolamine + palmitic acid
show the reaction diagram
-
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0
-
-
?
1-palmitoyl-lysophosphatidylethanolamine + H2O
glycerophosphoethanolamine + palmitate
show the reaction diagram
-
20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
-
-
?
1-palmitoyl-lysophosphatidylserine + H2O
glycerophosphatidylserine + palmitic acid
show the reaction diagram
-
3.8% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
1-palmitoyl-lysophosphatidylserine + H2O
glycerophosphatidylserine + palmitic acid
show the reaction diagram
-
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
-
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-, Q8GNM7
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
D2T083, -
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
palmitic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
palmitic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
palmitic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
weak
-
-
?
1-palmitoyl-sn-glycerol + H2O
palmitic acid + glycerol
show the reaction diagram
-
-
-
-
?
1-stearoyl-2-arachidonoyl-sn-glycerol
?
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphocholine + H2O
stearic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphocholine + H2O
stearic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphoethanolamine + H2O
stearic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphoethanolamine + H2O
stearic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphoethanolamine + H2O
stearic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
2,3-dioleoylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
2,3-dipalmitoyl-sn-glycero-1-phosphocholine + H2O
monopalmitoyl-sn-glycero-1-phosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
2,3-dipalmitoyl-sn-glycero-1-phosphocholine + H2O
monopalmitoyl-sn-glycero-1-phosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
2-acylglycerophosphocholine + H2O
fatty acid + glycerylphosphocholine
show the reaction diagram
-
-
-
-
?
2-acylglycerophosphocholine + H2O
fatty acid + glycerylphosphocholine
show the reaction diagram
-
2-acyl-sn-glycero-3-phosphocholine
-
-
?
2-acylglycerophosphoethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-
-
-
-
?
2-acylglycerophosphoglycerol + H2O
fatty acid + glcyerophosphoglycerol
show the reaction diagram
-
-
-
-
?
2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
D2T083, -
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-, Q939K7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
Q8NKD2
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-, Q8GNM7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
Q6P4A8
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
Q8NKD2
enzyme has also lysophospholipase transcylase activity
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a fatty acid
show the reaction diagram
-, Q7Z8L3
-
-
-
?
2-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
2-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
D2T083, -
relative activity: 0.45%
-
-
?
4-nitrophenyl decanoate + H2O
4-nitrophenol + decanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
show the reaction diagram
D2T083, -
relative activity: 7.56%
-
-
?
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
-
best substrate
-
-
?
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
D2T083, -
relative activity: 12.77%
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
D2T083, -
relative activity: 100%
-
-
?
4-nitrophenyl stearate + H2O
4-nitrophenol + stearate
show the reaction diagram
-
worst substrate
-
-
?
4-nitrophenyl stearate + H2O
4-nitrophenol + stearate
show the reaction diagram
D2T083, -
relative activity: 56.69%
-
-
?
C10:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C12:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C14:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:1-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:2-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C8:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
dioleoylphosphatidylcholine + H2O
?
show the reaction diagram
-
2% of the activity with dipalmitoylphosphatidylcholine
-
-
?
dioleoylphosphatidylethanolamine + H2O
?
show the reaction diagram
-
11% of the activity with dipalmitoylphosphatidylcholine
-
-
?
dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
ghrelin + H2O
des-acyl ghrelin + n-octanoic acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-, O59863
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
glycerophosphocholine + fatty acid
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
myristoyllysophosphatidylcholine is hydrolyzed 50% better than the longer chain palmitoyllysophosphatidylcholine
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
249% of the activity with phosphatidylcholine
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine
show the reaction diagram
-
lysophospholipase A activity
-
-
?
lysophosphatidylcholine + lysophosphatidylcholine
glycerophosphocholine + phosphatidylcholine
show the reaction diagram
-
-
-
?
lysophosphatidylcholine + lysophosphatidylcholine
glycerophosphocholine + phosphatidylcholine
show the reaction diagram
-
-
-
?
lysophosphatidylcholine + lysophosphatidylcholine
glycerophosphocholine + phosphatidylcholine
show the reaction diagram
-
slight preference for palmitate over stearate and laurate to be incorporated into phosphatidylcholine. No preference for palmitate over myristate
-
?
lysophosphatidylethanolamine + H2O
?
show the reaction diagram
-
-
-
-
?
lysophosphatidylethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-, Q7Z8L3
-
-
-
?
lysophosphatidylethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-
27% of the activity with phosphatidylcholine
-
-
?
lysophosphatidylglycerol + H2O
fatty acid + glycerol
show the reaction diagram
-
-
-
-
?
lysophosphatidylinositol + H2O
glycerophosphatidylinositol + a carboxylate
show the reaction diagram
-
78% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
monooleoylglycerol + H2O
oleic acid + glycerol
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
no activity detectable
-
-
-
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
Q8TZI9
substrate activity assay
-
-
?
p-nitrophenyl butyrate
p-nitrophenol + butyrate
show the reaction diagram
Q8TZI9
substrate activity assay
-
-
?
p-nitrophenyl palmitate
p-nitrophenol + palmitate
show the reaction diagram
Q8TZI9
substrate activity assay
-
-
?
palmitoyl-Gialpha1 + H2O
palmitic acid + Gialpha1
show the reaction diagram
-
thioesterase activity
-
?
palmitoyl-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
palmitoyl-peptide + H2O
palmitate + peptide
show the reaction diagram
Q6IAQ1
residue 3 to 14 of RGS4 protein with palmitoyl group attached at Cys12 of RGS4 protein
-
-
?
palmitoyl-SCoA + H2O
HS-CoA + palmitic acid
show the reaction diagram
-
-
-
?
palmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
-
-
-
-
?
phosphatidic acid + H2O
?
show the reaction diagram
-, Q7Z8L3
-
-
-
?
phosphatidic acid + H2O
?
show the reaction diagram
-
62% of the activity with phosphatidylcholine
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-, O59863
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
hydrolysis of the 1-acly ester bond and the 2-acyl ester bond, phospholipase B activity
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group
-
-
-
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
-
phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
-, Q939K7
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a fatty acid
show the reaction diagram
-, Q7Z8L3
-
-
-
?
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
show the reaction diagram
-
hydrolysis and acyl transfer
-
-
?
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a fatty acid
show the reaction diagram
-, Q7Z8L3
-
-
-
?
phosphatidylglycerol + H2O
lysophosphatidylglycerol + a carboxylate
show the reaction diagram
-
94.2% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
phosphatidylinositol + H2O
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylinositol + H2O
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
show the reaction diagram
-
-
-
-
-
phosphatidylinositol + H2O
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
show the reaction diagram
-
819% of the activity with phosphatidylcholine
-
-
?
phosphatidylinositol + H2O
lysophophatidylinositol + a carboxylate
show the reaction diagram
-, Q7Z8L3
-
-
-
?
phosphatidylinositol + H2O
lysophophatidylinositol + a carboxylate
show the reaction diagram
-
15.6% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
show the reaction diagram
-
-
-
-
?
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
show the reaction diagram
-
22% of the activity with phosphatidylcholine
-
-
?
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a fatty acid
show the reaction diagram
-, Q7Z8L3
-
-
-
?
platelet activating factor + H2O
platelet activating factor + a carboxylate
show the reaction diagram
-
8.9% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
sphingomyelin + H2O
?
show the reaction diagram
-
3% of the activity with dipalmitoylphosphatidylcholine
-
-
?
triacetin + H2O
?
show the reaction diagram
-
weak activity
-
-
?
tributyrin + H2O
?
show the reaction diagram
-
-
-
-
?
monopalmitoylglycerol + H2O
palmitic acid + glycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no transacylase activity
-
-
-
additional information
?
-
-
the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination
-
-
-
additional information
?
-
-
global regulatory proteins LetA and RpoS control lysophospholipase A
-
-
-
additional information
?
-
-
loss of lysophospholipase 3 increases atherosclerosis in apolipoprotein E-deficient mice. Even in a severe model of atherosclerosis, LCAT-like lysophospholipase exhibits a protective effect on atheroma formation at all stages and throughout the aortic tree in mice fed on normal diet
-
-
-
additional information
?
-
-
enzyme encoded by the gene plaB is a phospholipase A and a lysophospholipase A
-
-
-
additional information
?
-
-
lysophospholipase activity of lysophosphatidylcholine/transacylase is smaller than the transacylase activity
-
-
-
additional information
?
-
-
PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity
-
-
-
additional information
?
-
-, P0ADA1
substrate specificities of Escherichia coli thioesterase I/protease I/lysophospholipase L1 are governed by its switch loop movement
-
-
-
additional information
?
-
-
the LPL1 protein only possesses lysophospholipase and transacylase activity
-
-
-
additional information
?
-
-
combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
-
-
-
additional information
?
-
Q05315
combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
-
-
-
additional information
?
-
Q9P8L1
phospholipase B is necessary for the initiation of pulmonary infection by Cryptpococcus neoformans and for dissemination from the lung via the lymphatics. PLB1 plays a role in the binding of Cryptpococcus neoformans to host lung epithelial cells, possibly due to production of fatty acids from plasma memrane and/or surfactant by PLB activity
-
-
-
additional information
?
-
-
phospholipase B1 is implicated in transcellular metabolism of macrophage-derived lipids. During both logarithmic and stationary phases of growth, the physiologically relevant phospholipids, dipalmitoyl phosphatidylcholine (DPPC) and dioleoyl phosphatidylcholine, are taken up and metabolized via PLB1
-
-
-
additional information
?
-
-
hydrolyzes sn-1 esters in lysophosphatidylcholine and lysophosphatidic acid
-
-
-
additional information
?
-
-
acyl-CoA-binding protein 2 can bind both lysophosphatidylcholine and lysophospholipase 2 to promote the degradation of lysophosphatidylcholine in response to Cd2+-induced oxidative stress
-
-
-
additional information
?
-
-
LipC cleaves both ester linkages of the sn-1 and sn-2 positions of phospholipids (phospholipase B activity), no activity with tributyrin, tricaprilin or triolein
-
-
-
additional information
?
-
Legionella pneumophila JR32
-
global regulatory proteins LetA and RpoS control lysophospholipase A
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-acyl-2-lysophosphatidycholine + H2O
fatty acid + glycerophosphocholine
show the reaction diagram
Q9U8F2, -
-
-
-
?
1-monopalmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
-, Q8GNM7
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-, Q8GNM7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-, Q939K7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
Q8NKD2
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-, Q8GNM7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
Q6P4A8
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
-, Q939K7
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine
show the reaction diagram
-
lysophospholipase A activity
-
-
?
additional information
?
-
-
the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination
-
-
-
additional information
?
-
-
global regulatory proteins LetA and RpoS control lysophospholipase A
-
-
-
additional information
?
-
-
loss of lysophospholipase 3 increases atherosclerosis in apolipoprotein E-deficient mice. Even in a severe model of atherosclerosis, LCAT-like lysophospholipase exhibits a protective effect on atheroma formation at all stages and throughout the aortic tree in mice fed on normal diet
-
-
-
additional information
?
-
-
combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
-
-
-
additional information
?
-
Q05315
combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
-
-
-
additional information
?
-
Q9P8L1
phospholipase B is necessary for the initiation of pulmonary infection by Cryptpococcus neoformans and for dissemination from the lung via the lymphatics. PLB1 plays a role in the binding of Cryptpococcus neoformans to host lung epithelial cells, possibly due to production of fatty acids from plasma memrane and/or surfactant by PLB activity
-
-
-
additional information
?
-
-
phospholipase B1 is implicated in transcellular metabolism of macrophage-derived lipids. During both logarithmic and stationary phases of growth, the physiologically relevant phospholipids, dipalmitoyl phosphatidylcholine (DPPC) and dioleoyl phosphatidylcholine, are taken up and metabolized via PLB1
-
-
-
additional information
?
-
-
hydrolyzes sn-1 esters in lysophosphatidylcholine and lysophosphatidic acid
-
-
-
additional information
?
-
-
acyl-CoA-binding protein 2 can bind both lysophosphatidylcholine and lysophospholipase 2 to promote the degradation of lysophosphatidylcholine in response to Cd2+-induced oxidative stress
-
-
-
additional information
?
-
Legionella pneumophila JR32
-
global regulatory proteins LetA and RpoS control lysophospholipase A
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Al3+
-, Q7Z8L3
strong activation at pH 8.0, optimal concentration is 20 mM
Al3+
-, O59863
Ca2+, Fe3+ or Al3+ required
Ca2+
-
10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1
Ca2+
-
CaCl2, 10 mM, stimulates
Ca2+
-, O59863
Ca2+, Fe3+ or Al3+ required
CaCl2
-
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0
Co2+
-, Q7Z8L3
pH 8.0, activates
CoCl2
-
10 mM, stimulates
Cu2+
-, Q7Z8L3
pH 8.0, activates
Cu2+
D2T083, -
significantly inhibits enzyme activity
Cu2+
-
127% activity at 1 mM
CuSO4
-
10 mM, stimulates
Fe2+
-, Q7Z8L3
pH 8.0, activates
Fe2+
D2T083, -
significantly inhibits enzyme activity
Fe2+
-
124% activity at 1 mM
Fe3+
-, Q7Z8L3
pH 8.0, activates
Fe3+
-, O59863
Ca2+, Fe3+ or Al3+ required
Hg2+
D2T083, -
significantly inhibits enzyme activity
HgCl2
-
10 mM, stimulates
K3Fe(CN)6
-
activates
MgCl2
-
10 mM, stimulates
Mn2+
-, Q7Z8L3
pH 8.0, activates
Ni2+
D2T083, -
significantly inhibits enzyme activity
NiSO4
-
10 mM, stimulates
Zn2+
D2T083, -
significantly inhibits enzyme activity
Zn2+
-
relevant for activity
ZnCl2
-
10 mM, stimulates
MnCl2
-
10 mM, stimulates
additional information
-
Ca2+ independent phospholipase A2 activity
additional information
-, Q7Z8L3
activity at pH 3.0 is independent on metal ions
additional information
Q6IAQ1
activity independent of Ca2+ and Mg2+
additional information
-
not influenced by Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,12-bis(tributylammonium)dodecane
-
-
1,12-bis(trihexylammonium)dodecane
-
-
1,12-bis(triisopentylammonium)dodecane
-
-
1,12-bis(tripentylammonium)dodecane
-
-
1,12-bis(tripropylammonium)dodecane
-
-
1-palmitoyl lysophosphatidylcholine
Q6IAQ1
competitive inhibitor for acylprotein thioesterase activity
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
-
-
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
-
-
2-Hydroxy-5-nitrobenzyl bromide
-
-
3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide
-
-
AACOCF3
-
a trifluoromethyl ketone analog of arachidonic acid, uncompetitive
Albumin
-
-
-
alexidine dihydrochloride
-
specific inhibitor, 66% inhibition at 0.00025 mM
-
arachidonic acid
-
-
arachidonyl trifluoromethyl ketone
-
0.025 mM, approx. 50% inhibition
bis(p-nitrophenyl)phosphate
-
0.01 mM, 8% inhibition of lysophospholipase I and 61% inhibition of lysophospholipase II
bromoenol lactone
-
0.025 mM, approx. 30% inhibition
Ca2+
-
40% residual activity at 1 mM
Ca2+
-
CaCl2, reversed by EDTA
CaCl2
-
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity
calcium dipicolinate
-
71% residual activity at 1 mM
-
carnitine
-
10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1
Cetyltrimethylammonium bromide
-
-
Chlorpromazine
-
at low concentrations significantly stimulates phospholipase B activity of native enzyme, strong stimulation at high concentrations
chlorpyrifos pentyl oxon
-
0.000076 mM, 50% inhibition
Cu2+
-
little effect
D-alanine
-
94% residual activity at 1 mM
deoxycholate
-
0.1% sodium deoxycholate, complete inhibition of lysophospholipase activity
deoxycholate
-
soluble enzyme
deoxycholate
-
-
Dichlorvos
-
0.000028 mM, 50% inhibition
diisopropylfluorophosphate
-
-
diisopropylfluorophosphate
-
-
diisopropylfluorophosphate
-
-
dipalmitoyl phosphatidylcholine
-
0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1
dipalmitoylphosphatidylcholine
-
0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0
diphenyl fluorophosphinate
-
2 mM, 52% inhibition
dipicolinate
-
82% residual activity at 1 mM
Dithionitrobenzoate
-
-
dithiothreitol
-
10 mM, 50% inhibition of phospholipase activity
dithiothreitol
-
10 mM, 9% inhibition at pH 7.0, LPL1
dithiothreitol
-
78% residual activity at 5 mM
EDTA
-
81% residual activity at 5 mM
EGTA
-
10 mM, 15% inhibition at pH 7.0, LPL1
EPTC sulfoxide
-
0.2 mM, 51% inhibition
ethyl octylphosphonofluoridate
-
0.0000018 mM, 50% inhibition
ethyl octylphosphonofluoridate
-
0.00009 mM, 50% inhibition
Fe2+
-
-
-
Fe2+
-
-
-
Fe3+
-
1 mM, 95% inhibition of phospholipase activity, 80% inhibition of lysophospholipase activity
-
Fe3+
-, Q7Z8L3
at pH 3.0
-
Fe3+
-
-
-
Fe3+
-
partial
-
FeCl3
-
10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0
glutathione
-
membrane-bound enzyme, no inhibition of the soluble enzyme
Hg2+
-, Q7Z8L3
at pH 3.0
Hg2+
-
61% residual activity at 1 mM
Hg2+
-
little effect
Hg2+
-
5 mM, inhibits phospholipase B activity by 67%, no inhibition of lysophospholipase activity
Insulin
-
insulin reduces NRE mRNA levels in a dose-dependent manner in 3T3-L1 adipocytes
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetate
-
-
isopropyl dodecylphosphonofluoridate
-
0.000002 mM, 50% inhibition
isopropyl dodecylphosphonofluoridate
-
0.0000067 mM, 50% inhibition
L-alanine
-
93% residual activity at 1 mM
L-linoleoylcarnitine
-
-
L-palmitoylcarnitine
-
-
L-palmitoylcarnitine
-
-
L-palmitoylcarnitine
-
inhibits phosphatidylcholine formation
lysophosphatidic acid
-
-
lysophosphatidic acid
-
inhibition in a dose-dependent manner
lysophosphatidic acid
-
-
methyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14- tetraenylphosphonofluoridate
-
-
Mg2+
-
MgCl2, reversed by EDTA
Mn2+
-
67% residual activity at 1 mM
N-bromosuccinimide
-
-
n-dodecyl-benzodioxaphosphorine 2-oxide
-
0.000008 mM, 50% inhibition
n-dodecyl-benzodioxaphosphorine 2-oxide
-
0.000034 mM, 50% inhibition
n-octadecyl-benzodioxaphosphorine 2-oxide
-
0.000067 mM, 50% inhibition
NEM
-
little effect
NEM
-
soluble enzyme, no inhibition of the membrane-bound enzyme
NEM
-
transacylase activity
p-hydroxymercuribenzoate
-
-
palmitic acid
-
-
palmitoyl carnitine
-
0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0
palmitoyl-peptide
Q6IAQ1
competitive inhibitor for lysophospholipase activity
palmitoylcarnitine
-
10 mM, 70% inhibition of phospholipase activity
palmitoylcarnitine
-
transacylase activity
palmitoylcarnitine
-
-
phenyl N-benzylcarbamate
-
0.2 mM, 47% inhibition
phenylmethylsulfonyl fluoride
Q8TZI9
PMSF
phenylmethylsulfonyl fluoride
B0FLU6
PMSF
phenylmethylsulfonyl fluoride
-
72% residual activity at 1 mM
phosphatidic acid
-
0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0
phosphatidic acid
-
-
PMSF
-
little effect
S-n-octyl-benzodioxaphosphorine 2-oxide
-
0.000005 mM, 50% inhibition
SDS
-
0.05%, complete inhibition of phospholipase activity
SDS
-
soluble and membrane-bound enzyme
sphingomyelin
-
0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0
sulfite
-
enzymatic activity: 1.6%
tellurate
-
enzymatic activity: 19.8%
tellurite
-
enzymatic activity: 0%
-
tri-o-cresyl phosphate
B0FLU6
TOCP
tricyclodecan-9-yl-xanthogenate potassium
-
-
-
trifluoromethyl ketone
-
0.025 mM, approx. 40% inhibition
Triton X-100
-
50% inhibition of lysophospholipase activity
Triton X-100
-
0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0
Triton X-100
-
soluble enzyme
Triton X-100
-
monomolecular and micellar form of Triton X-100
Triton X-100
-
-
Zn2+
-
84% residual activity at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
minimizes loss of activity in presence of sulfhydryl reagent
carnitine
-
10 mM, activation to 121% of control at pH 5.0, LPL1
Chloramphenicol
-
stimulates phospholipase B activity
dithiothreitol
-
10 mM, activation to 122% of control at pH 5.0, LPL1
EGTA
-
10 mM, 11% activation of phospholipase B activity
glycerol
-
50% increase of lysophospholipase activity in the presence of 30% glycerol
palmitoyl carnitine
-
0.5 mM, stimulates to 157% of control at pH 7.0, LPL1; 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control
palmitoylcarnitine
-
stimulates hydrolase activity
pulmonary surfactant
-
enzyme activity significantly increases (28%) in the presence of 0.1 mg/ml pulmonary surfactant
-
Sodium deoxycholate
-
0.1%, stimulates
Sodium deoxycholate
-
0.1%, strongly stimulates phospholipase B activity
Triton X-100
-
stimulates phospholipase B activity
Triton X-100
-
stimulates membrane-bound enzyme
lysophosphatidylcholine
-
0.5 mM, activation of phospholipase B activity to 132% of control
additional information
-
pilD-dependent lysophospholipase
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1
-
1,2-didecanoyl-sn-glycero-3-phosphocholine
-
-
-
1.1
-
1,2-didecanoylphosphatidylcholine
Q6P4A8
-
2.9
-
1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
-
-
0.82
-
1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
-
0.19
-
1,2-dipalmitoylphosphatidylcholine
-
37C, pH 4.0
0.04498
-
1-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-2-lysophosphatidylcholine
-
Vmax: 103.5 micromol/h/mg protein
1.6
-
1-acyl-2-oleoyl-phosphatidylcholine
-
-
0.0676
-
1-arachidonoyl lysophosphatidylcholine
-
Vmax: 142.8 micromol/h/mg protein
0.0676
-
1-arachidonoyl-2-lysophosphatidylcholine
-
Vmax: 142.8 micromol/h/mg protein
0.04498
-
1-docosahexaenoyl lysophosphatidylcholine
-
Vmax: 103.5 micromol/h/mg protein
0.0992
-
1-hexanoyl lysophosphatidylcholine
-
Vmax: 121.3 micromol/h/mg protein
0.0992
-
1-hexanoyl-2-lysophosphatidylcholine
-
Vmax: 121.3 micromol/h/mg protein
0.1203
-
1-lauroyl lysophosphatidylcholine
-
Vmax: 141.6 micromol/h/mg protein
0.1203
-
1-lauroyl-2-lysophosphatidylcholine
-
Vmax: 141.6 micromol/h/mg protein
0.08892
-
1-linoleoyl lysophosphatidylcholine
-
Vmax: 97.7 micromol/h/mg protein
0.08892
-
1-linoleoyl-2-lysophosphatidylcholine
-
Vmax: 97.7 micromol/h/mg protein
0.127
-
1-myristoyl lysophosphatidylcholine
-
Vmax: 122.7 micromol/h/mg protein
0.127
-
1-myristoyl-2-lysophosphatidylcholine
-
Vmax: 122.7 micromol/h/mg protein
0.306
-
1-oleoyl lysophosphatidylcholine
-
Vmax: 0.113 micromol/h/mg protein
0.306
-
1-oleoyl-2-lysophosphatidylcholine
-
Vmax: 0.113 micromol/h/mg protein
0.0273
-
1-palmitoyl lysophosphatidylcholine
Q6IAQ1
-
0.19
-
1-palmitoyl lysophosphatidylcholine
-
Vmax: 90.9 micromol/h/mg protein
0.19
-
1-palmitoyl-2-lysophosphatidylcholine
-
Vmax: 90.9 micromol/h/mg protein
0.092
-
1-palmitoyl-lysophosphatidylcholine
-
37C, pH 4.0
0.076
-
1-palmitoyl-sn-glycero-3-phosphatidylcholine
-
-
0.0606
-
1-Palmitoyl-sn-glycero-3-phosphocholine
-
-
0.17
-
1-Palmitoyl-sn-glycero-3-phosphocholine
-
-
0.75
-
2-oleoyl-lysophosphatidylcholine
-
-
0.0065
-
ghrelin
-
-
-
0.132
-
lysolecithin
-
-
0.022
-
lysophosphatidylcholine
-
substrate concentration between 0.015-0.2 mM
0.03
-
lysophosphatidylcholine
-
-
0.05
-
lysophosphatidylcholine
-
-
0.083
-
lysophosphatidylcholine
-
at low substrate concentrations
0.26
-
lysophosphatidylcholine
-
at high substrate concentrations
0.00349
-
palmitoyl-peptide
Q6IAQ1
-
0.22
-
palmitoyllysophosphatidylglycerol
-
-
0.18
-
phosphatidylcholine
-
-
0.63
-
phosphatidylcholine
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0222
-
1-palmitoyl lysophosphatidylcholine
Q6IAQ1
for acylprotein thioesterase activity
0.0323
-
palmitoyl-peptide
Q6IAQ1
for lysophospholipase activity
0.16
-
tellurite
-
-
-
0.16
-
tellurite ion
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
1,12-bis(tributylammonium)dodecane
-
-
0.0075
-
1,12-bis(trihexylammonium)dodecane
-
-
0.0075
-
1,12-bis(triisopentylammonium)dodecane
-
-
0.0035
-
1,12-bis(tripentylammonium)dodecane
-
-
0.007
-
1,12-bis(tripropylammonium)dodecane
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.5
-
-
activity with palmitoyllysophosphatidylglycerol
1.34
-
-
-
1.62
-
Q6IAQ1
substrate 1-palmitoyl lysophosphatidylcholine
1.66
-
-
lysophospholipase II
2.3
-
-
activity with ghrelin
2.31
-
-
lysophospholipase I
2.448
-
-
-
2.65
-
-
-
10.5
-
-
hydrolysis of 1,2-dipalmitoylphosphatidylcholine
12.24
-
-
-
23.5
-
-
-
27.3
-
Q6IAQ1
substrate palmitoyl-peptide
28.72
-
-, Q7Z8L3
-
40
-
-
phospholipase B activity
77.68
-
-
hydrolase activity
117
-
-
fatty acid release
193
-
-
lysophospholipase activity
320.9
-
-
transacylase activity
459
-
-
phosphatidylcholine formation
559
-
-
hydrolysis of 1-palmitoyl-lysophosphatidylcholine
additional information
-
-
specific activity is 4-5 times higher in oxidative muscle than in glycolytic muscle
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
-
-, O59863
and a second optimum at pH 7.5
3
-
-, Q7Z8L3
two pH-optima: pH 3.0 and pH 7.5
4
-
-
hydrolysis of phosphatidylcholin and lysophosphatidylcholin
4
-
-
monoacylglycerols
4.5
5
-
phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1
5
-
-
lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1
5.5
8
-
lysophospholipase activity, long incubation time (5 min), LPL1
6
8
-
deacylation of 2-acyllysophosphatidylcholine, lysophospholipase I
6.5
7
-
hydrolysis of 1-palmitoyl-glycero-3-phosphorylcholine
6.5
-
-
and a second optimum at pH 8.0, membrane-bound enzyme
7
-
-
and a second optimum at pH 9.5, soluble enzyme
7.3
-
-
activity assay
7.4
-
Q6P4A8
-
7.4
-
Q6IAQ1
-
7.5
9
-
-
7.5
-
-, Q7Z8L3
two pH-optima: pH 3.0 and pH 7.5
7.5
-
-
assay at
7.5
-
-, O59863
and a second optimum at pH 2.0
8
-
-
in Semimembranosus proprius and Longissimus lumborum muscle
8
-
B0FLU6
activity assay
8
-
-
in Tris-HCl buffer
8
-
-
Tris buffer
8
-
-
and a second optimum at pH 6.5, membrane-bound enzyme
8
-
-
lysophospholipase II
8.5
-
-
assay at and optimal activity
8.5
-
-
deacylation of 2-acyllysophosphatidylcholine, lysophospholipase II
8.5
-
-
lysophospholipase II
9.5
-
-
and a second optimum at pH 7.0, soluble enzyme
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.2
3
-
pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity
2.5
6
-
approx. 50% of maximal phospholipase activity at pH 3.0 and pH 5.0, respectively
2.5
7
-
50-60% of maximal lysophospholipase activity at pH 3.0 and pH 5.5, respectively
4
11
-
41% of maximal activity at pH 5.0, 79% of maximal activity at pH 6, Semimembranosus proprius muscle, 33% of maximal acitivity at pH 5, 75% of maximal activity at pH 6, Longissimus lumborum muscle
4
7
-
pH 4.0: about 55% of maximal activity, pH 7.0: about 60% of maximal activity
4
7
-
pH 4.0: about 45% of maximal activity, pH 7.0: about 85% of maximal activity, lysophosphatidylcholine transacylase activity, pH 4.0: about 50% of maximal activity, pH 7.0: about 80% of maximal activity, lysophosphatidyl hydrolase activity
4
8
-
pH 4.0: about 65% of maximal activity, pH 8.0: about 40% of maximal activity, production of phosphatidylcholine
4.2
6
-
pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1
5
9
-
pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity
5
9.3
-
pH 5.0: about 30% of maximal activity, pH 9.3: about 35% of maximal activity
7
-
-
no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
lysophospholipase activity
25
-
B0FLU6
activity assay
30
-
-
assay at
37
-
-
phospholipase activity
37
-
-
; phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures
37
-
-
activity assay
37
-
-
assay at
40
-
-
transacylase activity
40
-
-, Q7Z8L3
at pH 3.0
40
-
-
lysophospholipase reaction proceeds even at 0C and its rate increases with temperature up to roughly 50C, the optimal temperature is near 40 C
70
-
Q8TZI9
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
50
-
25C: about 70% of maximal activity, 50C: about 90% of maximal activity
30
80
Q8TZI9
conformational changes are negligible over the temperature range of 30-80C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
-
-
2D-electrophoresis
3.9
-
-
isoelectric focusing
5.1
-
-
isoelectric focusing, 63000 Da species
6.05
-
Q9U8F2, -
determined by isoelectric focusing, SDS-PAGE, extract of adult Schistosoma japonicum worm
6.3
-
-
isoelectric focusing, isoenzyme LPL1
6.3
-
-
isoelectric focusing 52000 Da species
6.4
-
Q8NKD2
predicted from nucleotide sequence
7.06
-
Q9U8F2, -
predicted
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
transcript is isolated from the egg stage
Manually annotated by BRENDA team
-
oxidative and glycolytic muscles
Manually annotated by BRENDA team
Q3TTY0
primary cultures of mouse neural cells. PLB activity is present in a variety of cultured mouse neural cell types but that acute loss of this activity is not cytotoxic
Manually annotated by BRENDA team
-
macrophage-like
Manually annotated by BRENDA team
Q9WTL7
isoform lysophospholipase I only
Manually annotated by BRENDA team
-
aleurone; endosperm
Manually annotated by BRENDA team
-
in vitro storage for 48 h does not affect activity
Manually annotated by BRENDA team
-
brush-border membrane
Manually annotated by BRENDA team
-
hLysoPLA I expression is induced by phorbol 12-myristate 13-acetate
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
two-third of the enzyme is particulate, one-third is cytoplasmic
Manually annotated by BRENDA team
Q8GNM7
major secreted lysophospholipase A
-
Manually annotated by BRENDA team
B0XZV8, B0Y665, Q9P8P4
;
-
Manually annotated by BRENDA team
-
tightly bound to membrane
Manually annotated by BRENDA team
-
enzyme is also located in mitochondria even in the absence of prenylation
Manually annotated by BRENDA team
-
two-third of the enzyme is particulate, one-third is cytoplasmic
-
Manually annotated by BRENDA team
D2T083, -
protein contians a putative peroxisomal targeting sequence
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21000
-
-
lysophospholipase I, gel filtration
23000
-
Q9U8F2, -
determined by Western blot analysis, extract of adult Schistosoma japonicum worm; immunoblot analysis, single band in both membrane-associated and soluble tissue fractions of adult schistosomes
23000
-
Q9U8F2
immunoblot analysis, single band in both membrane-associated and soluble tissue fractions of adult schistosomes
23000
-
-
lysophospholipase II, gel filtration
24840
-
Q9U8F2, -
calculated; calculated from cDNA
24840
-
Q9U8F2
calculated from cDNA
25000
-
-
gel filtration
26000
-
Q9U8F2, -
determined by SDS-PAGE, recombinant 6-His-tagged lysophospholipase; SDS-PAGE, recombinant protein
26000
-
Q9U8F2
SDS-PAGE, recombinant protein
26000
-
-
lysophospholipase I, SDS-PAGE
29000
-
Q8TZI9
determined by SDS-PAGE
30000
-
-
lysophospholipase I, gel filtration
32040
-
Q8TZI9
calculated
35500
-
D2T083, -
calculated from cDNA
42000
-
Q6P4A8
determined by SDS-PAGE and Western Blot analysis
49000
-
-
gel filtration
52000
-
-
a 52000 Da species and a 63000 Da species is detected by SDS-PAGE.The 52000 Da species is a truncated version of the 63000 Da protein
57000
-
-
lysophospholipase II, SDS-PAGE
58000
-
-
gel filtration
61000
-
-
SDS-PAGE, Flag-tagged fusion protein
63000
-
-
a 52000 Da species and a 63000 Da species is detected by SDS-PAGE. The 52000 Da species is a truncated version of the 63000 Da protein
63000
-
-
gel filtration
65000
-
-
SDS-PAGE
90000
-
-
gel filtration
116000
-
-
gel filtration
130000
-
Q6P4A8
determined by gel filtration
140000
232000
-
native PAGE
160000
180000
-
gel filtration
220000
-
-
isoenzyme LPL1, gel filtratiin
275000
-
-
isoenzyme PLB1, gel filtratiin
280000
420000
-
gel filtration
300000
-
-
gel filtration
330000
-
-
gel filtration
additional information
-
-
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 70000-90000, SDS-PAGE
?
Q8NKD2
x * 48000, deduced from nucleotide sequence; x * 97000-140000, SDS-PAGE, immunoblot
?
-
x * 28000, SDS-PAGE
?
-
x * 65800, deduced from nucleotide sequence; x * 66000, SDS-PAGE, immunoblot
?
-, Q7Z8L3
x * 90000-110000, SDS-PAGE
?
Q6IAQ1
x * 25000, SDS-PAGE (52000 for GST-fusion protein)
?
-
x * 37000, SDS-PAGE; x * 40900, His-tagged enzyme, SDS-PAGE
?
-
x * 145000, phospholipase B2, SDS-PAGE; x * 220000, phospholipase B1, SDS-PAGE
?
-
x * 106000, modified enzyme that is generated by the introduction of some nicks into the native type of enzyme, SDS-PAGE; x * 95000, native enzyme form, SDS-PAGE
?
-
x * 40000, PLP I, SDS-PAGE
?
-
x * 38500, SDS-PAGE
?
-
x * 23000, SDS-PAGE
?
-
x * 78000, SDS-PAGE
?
-
x * 39500, SDS-PAGE
?
-
x * 37000, SDS-PAGE
?
-
x * 35000, SDS-PAGE
?
-
x * 24000, lysophospholipase I, SDS-PAGE; x * 63000, lysophospholipase II, SDS-PAGE
?
-
x * 24708, calculation from nucleotide sequence
?
-
x * 84000, SDS-PAGE
?
-
x * 27000, lysophospholipase I, SDS-PAGE; x * 28000, lysophospholipase II, SDS-PAGE
decamer
-
10 * 66000, isoenzyme LPL1, SDS-PAGE
dimer
-
2 * or 3 x * 100000-150000, SDS-PAGE
homodimer
Q8TZI9
prediction, based on the determination of the hydrodynamic radius by dynamic light scattering measurements
monomer
-
1 * 24669, deduced from amino acid sequence
monomer
-
1 * 67000, SDS-PAGE
monomer
-
1 * 22000, lysophospholipase I, SDS-PAGE; 1 * 23000, lysophospholipase II, SDS-PAGE
monomer
-
1 * 41000, SDS-PAGE
trimer
-
2 * or 3 x * 100000-150000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
side-chain modification
-
glycoprotein
side-chain modification
-
glycoprotein; high level of N-glycosylation
glycoprotein
-, Q7Z8L3
-
side-chain modification
-
-
side-chain modification
-
10-12% carbohydrate; glycoprotein
glycoprotein
-
lysophosphatidylcholine/transacylase
side-chain modification
-, O59863
glycoprotein
side-chain modification
-
contains 30% neutral sugars; glycoprotein
side-chain modification
-
glycoprotein
side-chain modification
-
glycoprotein; phospholipase 2 contains 0.0097 mM mannose per mg of protein; phospholipase B1 contains 0.0207 mM mannose per mg of protein; phospholipase B1 contains 0.0224 mM carbohydrate per mg protein; phospholipase B2 contains 0.0104 mM carbohydrate per mg protein
side-chain modification
-
glycoprotein
side-chain modification
-
-
side-chain modification
-
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystal structure of the multifunctional thioesterase I/protease I/lysophospholipase L1 in complex with octanoic acid and of the L109P mutant enzyme in complex with octanoic acid
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.2
6.5
-
stable between pH 2.2 and pH 6.5
6
8.5
-
unstable below pH 6.0 and above pH 8.5
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
4
-
half-life in presence of 10 mM lysophosphatidylcholine is 72 h
4
10
D2T083, -
enzyme is a psychrophilic enzyme and retains about 50% of its maximal activity at 10C and 40% at 4C
37
-
-
t1/2 is 3 min
50
-
-
45% loss of lysophospholipase and transacylase activity, complete loss of phospholipase activity
50
-
D2T083, -
inactivation above 50C
50
-
-
about 60% loss of activity of the Ca2+-depleted enzyme after 2 h, about 80% loss of activity of the Ca2+-containing enzyme after 2 h
55
-
-
30 min, 95% loss of activity
55
-
-
5 min, complete inactivation
60
70
-
less than 20% activity remains after 30 min incubation at above 60C
60
-
-
complete loss of lysophospholipase and transacylase activity
70
-
-
complete inactivation above
80
-
-, Q7Z8L3
complete loss of activity after incubation above 80C
85
-
Q8TZI9
begins to unfold above 85C, with unfolding remaining incomplete even at 95C
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable upon purification, enzyme activity is lost during storage at 4C or -70C
Q8NKD2
-20C, in absence of glycerol, the enzyme loses all of its activity during the freezing and thawing process
-
60% loss of activity after 14 h dialysis in the absence of lysophosphatidylcholine, at 0-4C
-
approximately 50% loss of activity after one 24 h freeze-thawing
-
repeated freezing and thawing causes significant loss of activity
-
unstable in absence of glycerol and 2-mercaptoethanol
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetonitrile
Q8TZI9
25%, substrate p-nitrophenyl palmitate, relative activiy 58%
Ethanol
Q8TZI9
25%, substrate p-nitrophenyl palmitate, relative activiy 39%
Methanol
Q8TZI9
25%, substrate p-nitrophenyl palmitate, relative activiy 20%
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, 50 mM Tris/HCl, pH 7.4, more than one year, stable
Q6IAQ1
-20C, enzyme concentration: 0.01-0.025 mg/ml, 60-70% glycerol, stable for several months
-
4C, stable for several weeks
-
-45C, less than 10% loss of activity during several months
-
-80C, stable for at least 4 weeks
-
-80C, stable for at least 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography
-
His bind resin column chromatography
-
isoenzyme PLB1
-
ammonium sulfate, phenyl-Sepharose, Uno Q-1, Superose 12 HR, Superdex 200
-
isoenzyme LPL1
-
GST affinity chromatography, GST-tag cleaved from GST-fusion protein
Q6IAQ1
using membranes and an anti-Flag column
-
using Sephadex G-75, Mono-S cation-exchange and hydroxyapatite columns
Q6P4A8
lysophosphatidylcholine/transacylase
-
cytosolic fractions of COS7 cell extracts containing His-tagged NRE are loaded onto a Q-Sepharose column
-
recombinant ExoU
-
using a Ni-NTA affiniity column
Q8TZI9
recombinant
-
by affinity chromatography on Ni-NTA Superflow resin; using Ni-NTA chromatography
Q9U8F2, -
using Ni-NTA chromatography
Q9U8F2
by procedures employing acetic acid precipitation, 1-butanol solubilization and ammonium sulfate fractionation, and chromatographies
-
lysophospholipase I and II
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Arabidopsis thaliana seedlings
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli and Saccharomyces cerevisiae
Q8NKD2
mutant cDNAs (R108A, S146A, and D392A) are expressed in Saccharomyces cerevisiae
-
expressed as an N-terminal myc-tagged and C-terminal Flag-tagged fusion protein in HeLa or HEK293 cells. C-terminal Flag-tag is inserted into the prenylation site
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in HL-60 cells
-
GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell
Q6IAQ1
expression in Escherichia coli, enzyme encoded by the gene plaB is a phospholipase A and a lysophospholipase A
-
expression in Escherichia coli, overexpression in Legionella pneumophila completely protects from the toxic effects of lysophosphatidylcholine
Q8GNM7
expression in Escherichia coli
-
expression in Escherichia coli as a secreted, soluble, non-tagged, mature construct
-
expression in Escherichia coli
-
into the vectors pcDNA4HisMax and pEGFP-C1
-
expression in Escherichia coli
-
into the vector pGEX-KG and subsequently into pET23a for expression in Escherichia coli BL21DE3 pLysS cells
Q8TZI9
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
Q9U8F2
expressed in Escherichia coli; expressed in Escherichia coli as a His-tagged fusion protein; into the vector pQE30 for expression in Escherichia coli cells
Q9U8F2, -
cloned and expressed in Escherichia coli
D2T083, -
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
lysoPL2 mRNA expression in shoots is up-regulated by Zn2+, and H2O2 (but not by Pb2+, Cd2+ and Cu2+), while roots are only up-regulated by H2O2
-
lipopolysaccharide suppresses APT1 mRNA and protein expression
-
APT1 is released at high levels from RAW264.7 macrophage-like cells into the culture medium after stimulation with lipopolysaccharide, the mRNA level of lysophospholipase II in RAW264.7 cells is much less than APT1 and not changed by lipopolysaccharide treatment
Q9WTL7
lipopolysaccharide suppresses APT1 mRNA and protein expression
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S11A
-
the amount of free fatty acids in the mutant spores is about 35fold less than in the wild type spores
D392A
-
inactive mutant protein
R108A
-
inactive mutant protein
S146A
-
inactive mutant protein
S119A
Q6IAQ1
part of the catalytic triad, no activity
D406A
-, O59863
loss of enzymatic activity
R112A
-, O59863
loss of enzymatic activity
D174A
-
inactive
L109P
-
mutation abolishes switch loop movement
additional information
-
C-terminal Flag-tag is inserted into the prenylation site. Mutant enzyme shows lysophospholipase activity indicating that C-terminal processing is not essential for activity
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
renaturation of heat denatured enzyme
-
the enzyme is refolded from inclusion bodies
Q8TZI9
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
cryptococcal phospholipase B1 is a virulence factor
medicine
Q05315
the phospholipases and lysophospholipases expressed by eosinophils or other airway cells may represent therapeutic targets for blocking surfactant degradation, dysfunction and peripheral airway closure in asthma
medicine
-
the cytotoxic potential and signalling properties enable Legionella pneumophila PLA, LPLA enzymes to act as bacterial virulence factors, involved in severe lung disease
medicine
-
LLPL activity is anti-atherogenic and the regulation of this enzyme might be a novel drug target for the treatment of atherosclerosis
medicine
-
the phospholipases and lysophospholipases expressed by eosinophils or other airway cells may represent therapeutic targets for blocking surfactant degradation, dysfunction and peripheral airway closure in asthma
medicine
Q9U8F2, -
schistosome lysophospholipase may represent a new target for anti-schistosomal-chemotherapy; Schistosome lysophospholipase may represent a new target for anti-schistosomal chemotherapy