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Information on EC 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase and Organism(s) Homo sapiens

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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
lp-pla2, lipoprotein-associated phospholipase a2, paf acetylhydrolase, acetylhydrolase, plasma paf-ah, platelet-activating factor acetylhydrolase, paf-acetylhydrolase, pla2g7, lppla2, pafah, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine: acetylhydrolase
-
-
-
-
1-alkyl-2-acetylglycerophosphocholine esterase
-
-
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1-alkyl-2-acetyllecithin deacetylase
-
-
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2-acetyl-1-alkylglycerophosphocholine esterase
-
-
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acetylhydrolase
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-
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alkylacetyl-GPC:acetylhydrolase
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aspirin hydrolase
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blood platelet-activating factor-acetyl hydrolase
-
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calcium-independent serine lipase Lp-PLA2
-
-
deacetylase, 1-alkyl-2-acetyllecithin
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HpPAF-AH
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HSD-PLA2
-
-
-
-
intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 1
-
intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 2
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intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 3
-
intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 4
-
intracellular platelet-activating factor acetylhydrolase alpha 2 subunit variant 5
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LDL-associated phospholipase A2
-
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LDL-PLA(2)
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lipoprotein associated phospholipase A2
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lipoprotein-associated phospholipase A2
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lipoprotein-associated PLA2
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lipoprotein-associated platelet-activating factor acetylhydrolase
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lipoprotein-PLA2
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Lissencephaly-1 protein
-
-
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-
PAF 2-acylhydrolase
-
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-
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PAF acetylhydrolase
PAF acetylhydrolase I
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PAF-acetylhydrolase
PAF-acetylhydrolase II
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PAF-AH
PAF-AH alpha
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PAF-AH II
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-
PAFAH alpha
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phosphatide 2-acylhydrolase
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PLA2G7 gene
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plasma lipoprotein-associated phospholipase A2
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plasma PAF acetylhydrolase
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plasma PAF-AH
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plasma platelet - activating factor - acetylhydrolase
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plasma platelet activating factor acetylhydrolase
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plasma platelet activating factor-acetylhydrolase
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plasma platelet-activating factor acetylhydrolase
platelet-activating factor acetyl-hydrolase
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-
platelet-activating factor acetylhydrolase
platelet-activating factor acetylhydrolase II
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platelet-activating factor acetylhydrolyase
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-
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platelet-activating factor-acetylhydrolase
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platelet-activating-factor acetylhydrolase
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pPAF-AH
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Serine dependent phospholipase A2
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SpV-4
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type I PAF acetylhydrolase
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type I platelet-activating factor acetylhydrolase
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VIIA phospholipase A2
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of carboxylic ester
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine acetohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
76901-00-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-didecanoyl-sn-glycerol + H2O
?
show the reaction diagram
-
-
-
?
1,2-dioctanoyl-sn-glycerol + H2O
?
show the reaction diagram
-
-
-
?
1,2-dioctanoylethyleneglycol + H2O
?
show the reaction diagram
-
-
-
?
1-acetyl-2-hexadecyl-sn-glycero-3-phosphocholine + H2O
2-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
?
1-acyl-2-linoleoyl-glycero-phosphocholine + H2O
2-linoleoyl-glycero-phosphocholine + acylic acid
show the reaction diagram
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
1-decanoyl-rac-glycerol + H2O
decanoate + glycerol
show the reaction diagram
-
-
-
?
1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O
1-hexadecyl-sn-glycerol-3-phosphocholine + acetate
show the reaction diagram
-
i.e. PAF
-
?
1-hexadecyl-2-glutaryl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-glycero-3-phosphocholine + glutarate
show the reaction diagram
-
-
-
?
1-myristoyl-2-(4-nitrophenyl succinyl)phosphatidylcholine + H2O
1-myristoyl-phosphatidylcholine + 4-nitrophenyl succinate
show the reaction diagram
-
artificial substrate
4-nitrophenyl succinate spontaneously degrades and liberates 4-nitrophenol
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
regulation of platelet-activating factor
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1-O-alkyl-2-butyryl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + butanoate
show the reaction diagram
-
-
-
?
1-O-alkyl-2-propionyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + propanoate
show the reaction diagram
-
-
-
?
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycerol-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
1-octanoyl-rac-glycerol + H2O
octanoate + glycerol
show the reaction diagram
-
-
-
?
1-oleoyl-2-acetyl-sn-glycerol + H2O
1-oleoyl-sn-glycerol + acetate
show the reaction diagram
-
-
-
?
1-palmitoyl-2(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + 5-oxopentanoate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O
5-oxopentanoate + 1-palmitoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + glutarate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-oxopentanoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + 2-oxopentanoate
show the reaction diagram
-
-
-
?
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-O-alkyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
i.e. platelet-activation factor, hydrolysis of sn-2 ester bond of platelet-activating factor PAF and related inflammatory phospholipids, no activity with the erythrocyte isozyme
-
?
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O
1-O-alkyl-sn-glycero-3-phosphoethanolamine + acetate
show the reaction diagram
-
-
-
?
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
show the reaction diagram
-
-
-
?
2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine + H2O
1-octadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
?
2-acetyl-3-octadecyl-sn-glycero-1-phosphocholine + H2O
3-octadecyl-sn-glycero-1-phosphocholine + acetate
show the reaction diagram
-
-
-
?
2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 40% of the activity with PAF
-
?
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
i.e. platelet-activation factor
-
?
2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 73% of the activity with PAF
-
?
2-thio platelet-activating factor + H2O
?
show the reaction diagram
-
substrate activity assay
-
-
?
2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 17% of the activity with PAF
-
?
4-(phenylthio)-N-(4-phenyl-2-oxobutyl)azetidin-2-one + H2O
thiophenol + 3-oxopropanoic acid + 1-amino-4-phenyl-2-butanone
show the reaction diagram
-
-
-
?
butyl acetate + H2O
butanol + acetate
show the reaction diagram
-
-
-
?
dibutanoyl-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
dibutyryl-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
didecanoyl-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
dioctanoyl-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
F2-isoprostane phospholipid + H2O
F2-isoprostane + phospholipid
show the reaction diagram
-
plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response
-
-
?
F2-isoprostane-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
lipoteichoic acid + H2O
?
show the reaction diagram
-
PAF-AH removes one acyl chain at the sn-2 position of pneumococcal lipoteichoic acid and produces a monoacyl-LTA that is inactive against mouse cells
-
-
?
methyl butyrate + H2O
methanol + butyrate
show the reaction diagram
-
-
-
?
octyl acetate + H2O
octanol + acetate
show the reaction diagram
-
-
-
?
oxidized phospholipids + H2O
?
show the reaction diagram
phospholipids + H2O
?
show the reaction diagram
platelet activating factor + H2O
acetate + deacetylated platelet activating factor
show the reaction diagram
-
erythrocyte and plasma PAF acetylhydrolases
-
-
?
platelet-activating factor + H2O
?
show the reaction diagram
platelet-activating factor + H2O
lyso-platelet-activating factor + acetate
show the reaction diagram
-
-
-
-
?
triacetylglycerol + H2O
diacetylglycerol + acetate
show the reaction diagram
-
-
-
?
tributyrylglycerol + H2O
dibutyrylglycerol + butyrate
show the reaction diagram
-
-
-
?
trioctanoylglycerol + H2O
dioctanoylglycerol + octanoate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
regulation of platelet-activating factor
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylation
-
?
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF
-
?
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
show the reaction diagram
-
-
-
?
F2-isoprostane phospholipid + H2O
F2-isoprostane + phospholipid
show the reaction diagram
-
plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response
-
-
?
oxidized phospholipids + H2O
?
show the reaction diagram
phospholipids + H2O
?
show the reaction diagram
-
hydrolyzes sn-2-ester bonds of fragmented or oxidized phospholipids, enzyme deficiency results in an increased risk of stroke, coronary artery disease, atherosclerotic occlusive disease, and abdominal aortic aneurysm
-
-
?
platelet activating factor + H2O
acetate + deacetylated platelet activating factor
show the reaction diagram
-
erythrocyte and plasma PAF acetylhydrolases
-
-
?
platelet-activating factor + H2O
?
show the reaction diagram
platelet-activating factor + H2O
lyso-platelet-activating factor + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-O-alkyl-2-acetyl-sn-glycero-3-phosphodimethylethanolamine
-
-
1-O-alkyl-2-acetyl-sn-glycero-3-phosphoethanolamine
-
-
1-O-alkyl-2-acetyl-sn-glycero-3-phosphomonomethylethanolamine
-
-
1-O-hexadecanoyl-sn-glycero-3-phosphocholine
-
competitive inhibition of acetylhydrolase activity
2-N-methylcarbamyl platelet-activating factor
-
weak inhibition
-
4-(2-aminoethyl)benzenesulfonyl fluoride
-
i.e. pefabloc, inhibits both acetylhydrolase and transacetylase activities completely at 0.05 mM for the native enzyme, and at 1 mM for the recombinant enzyme
4-(phenylthio)-N-(4-phenyl-2-oxobutyl)azetidin-2-one
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
atorvastatin
-
reduces LDL-associated enzyme activity by reduction of LDL amount, does not reduce enzyme secretion by macrophages
benzodioxaphosphorin 2-oxides
-
weak or no inhibition
-
chlorpyrifos oxon
-
ethyl- and methyl-derivative
corticoids
-
regulatory function on the enzyme form from HL-60 cells
-
CV 3988
-
-
diazoxon
-
weak inhibition
diethyldicarbonate
-
-
diisopropylfluorophosphate
dithioerythritol
-
-
fenofibrate
-
decreases total plasma and LDL_associated enzyme activity but does not influence HDL-associated enzyme activity
IFN-gamma
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
interleukin-1alpha
-
regulatory function on the enzyme form from decidual macrophages
-
interleukin-1beta
-
regulatory function on the enzyme form from decidual macrophages
-
interleukin-8
-
regulatory function on the enzyme form from decidual and monocyte-derived macrophages
-
iodoacetamide
-
17% inhibition at 5 mM
lipopolysaccharides
-
regulatory function on the enzyme form from decidual macrophages, monocyte-derived macrophages, and HL-60 cells
-
methoxy arachidonyl fluorophosphonate
-
type 1 PAF acetylhydrolase is irreversibly inhibited
methyl arachidonyl fluorophosphonate
n-alkyl methylphosphonofluoridates
-
derivatives with chain length C13-C18 are highly potent in vitro inhibitors
-
p-bromophenacyl bromide
-
-
p-bromophenacylbromide
-
60% inhibition at 5 mM, isozyme II from cerebrospinal fluid
p-nitroguanidinobenzoate
-
-
pefabloc
-
-
phenylmethylsulfonyl fluoride
-
13 mM, 50% inhibition of seminal plasma enzyme
platelet activating factor
-
competitive
PMSF
-
80% inhibition at 5 mM, complete inhibition at 10 mM, isozyme II from cerebrospinal fluid
SB-677116
-
-
-
SB222657
-
-
Sulfonyl fluorides
-
weak inhibition
tribufos
-
inhibition at 0.1 mM
tumor growth factor-beta
-
regulatory function on the enzyme form from HL-60 cells
-
tumor necrosis factor-alpha
-
regulatory function on the enzyme form from decidual macrophages
-
additional information
-
no inhibition of the isozyme II from cerebrospinal fluid by NaF, Mg2+, Ca2+, and EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
corticoids
-
regulatory function on the enzyme form from HL-60 cells
-
dithiothreitol
-
1.3fold increase in activity, isozyme II from cerebrospinal fluid
dyslipidemia
-
induces an increase in total plasma enzyme activity and alters the enzyme distribution between proatherogenic apoB- and antiatherogenic apo L-containing lipoproteins by increasing the enzyme activity associated with apoB-containing lipoproteins, atorvastin and fenofibrate can restore the normal level by reduction of LDL amount
-
GM-CSF
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
M-CSF
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
platelet-activating factor
-
regulatory function on the enzyme form from HepG2 cells and monocyte-derived macrophages, no effect on erythrocytes and erythrocyte isozyme
progestin
-
regulatory function on the enzyme form from HL-60 cells
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011
1,2-didecanoyl-sn-glycerol
-
plasma isozyme, pH 7.5, 22°C
0.005
1,2-dioctanoyl-sn-glycerol
-
below, plasma isozyme, pH 7.5, 22°C
0.005
1,2-dioctanoylethyleneglycol
-
below, plasma isozyme, pH 7.5, 22°C
0.005
1-acetyl-2-hexadecyl-sn-glycero-3-phosphocholine
-
below, plasma isozyme, pH 7.5, 22°C
0.086
1-decanoyl-rac-glycerol
-
plasma isozyme, pH 7.5, 22°C
0.0052 - 0.392
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine
1.4
1-octanoyl-rac-glycerol
-
plasma isozyme, pH 7.5, 22°C
0.006
1-oleoyl-2-acetyl-sn-glycerol
-
plasma isozyme, pH 7.5, 22°C
0.0193 - 0.0431
1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine
0.012 - 0.0137
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
-
0.005
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
below, plasma isozyme, pH 7.5, 22°C
0.005
2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
-
below, plasma isozyme, pH 7.5, 22°C
63
butyl acetate
-
plasma isozyme, pH 7.5, 22°C
4.7
dibutanoyl-phosphocholine
-
plasma isozyme, pH 7.5, 22°C
0.005
didecanoyl-phosphocholine
-
below, plasma isozyme, pH 7.5, 22°C
0.047
dioctanoyl-phosphocholine
-
plasma isozyme, pH 7.5, 22°C
0.0000045 - 0.000057
F2-isoprostane-phosphocholine
143
methyl butyrate
-
plasma isozyme, pH 7.5, 22°C
0.055
octyl acetate
-
plasma isozyme, pH 7.5, 22°C
2.1
platelet activating factor
-
native enzyme, pH 7.2, 37°C
131
triacetylglycerol
-
plasma isozyme, pH 7.5, 22°C
0.136
tributyryl-glycerol
-
plasma isozyme, pH 7.5, 22°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 8.1
1-acetyl-2-hexadecyl-sn-glycero-3-phosphocholine
0.25 - 3
1-oleoyl-2-acetyl-sn-glycerol
0.34 - 24.6
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
0.68 - 6.08
2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
0.28 - 6.08
octyl acetate
1.7 - 37
triacetylglycerol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29 - 0.47
platelet activating factor
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0003
1-amino-4-[phenanthrene-9-yl-amino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
Homo sapiens
-
potent inhibitor, IC50: 0.0003 mM, almost complete inhibition at 0.01 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000001
-
acetylhydrolase activity mg tissue, mammary artery, plus Pefablok
0.0000003
-
acetylhydrolase activity mg tissue, aorta, plus Pefablok
0.0000014
-
acetylhydrolase activity mg tissue, mammary artery
0.0000028
-
acetylhydrolase activity mg tissue, aorta
0.000008
-
cytosolic fraction, nonpregnant uterus
0.000073
-
cytosolic fraction, pregnant uterine myometrium
0.000221
-
cytosolic fraction, myoma
0.00123
-
-
0.015
-
transacetylase activity
0.022
-
acetylhydrolase activity
0.805
-
purified from erythrocytes
4.2
-
purifed enzyme using 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine as substrate
9.46
-
plasma enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
plasma enzyme
7.2
-
assay at
7.5
-
assay at
7.8
-
plasma enzyme
8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
broad range, inactivation below pH 4.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
25
-
assay at
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
atherogenic aorta
Manually annotated by BRENDA team
-
nonatherogenic mammary artery
Manually annotated by BRENDA team
-
platelets contain the plasma type as well as the intracellular type II PAF-AH. Platelets contain high levels of the mRNA of plasma PAF-AH, whereas only a small quantity of the type II PAF-AH mRNA is detected. Platelets secrete the plasma type of platelet-activating factor acetylhydrolase primarily associated with microparticles
Manually annotated by BRENDA team
-
isozyme II, of children with meningitis
Manually annotated by BRENDA team
-
monocyte-derived cells show very low enzyme expression
Manually annotated by BRENDA team
-
secretion of plasma isozyme
Manually annotated by BRENDA team
-
secretion of plasma isozyme
Manually annotated by BRENDA team
-
epidermal, isozyme II, lower amount compared to sebaceous gland
Manually annotated by BRENDA team
-
Kaposi's sarcoma cell line, implanted into SCID mice which then show reduced vascularization and growth
Manually annotated by BRENDA team
-
MM6 cell
Manually annotated by BRENDA team
-
enzyme activity increases during differentiation, only monocyte-derived dendritic cells show highly reduced enzyme expression
Manually annotated by BRENDA team
-
at least 2 isozymes I and II, increased amount of isozyme I beta-subunit
Manually annotated by BRENDA team
-
at least 2 isozymes I and II
Manually annotated by BRENDA team
-
isozyme II, high amount
Manually annotated by BRENDA team
-
the activity is significantly and negatively correlated with sperm motility. Platelet-activating factor acetylhydrolase serves as a decapacitation factor
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
isozyme II
Manually annotated by BRENDA team
-
isozyme I activity is lower in pregnant uterus compared to the nonpregnant one
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
-
higher expression of mouse compared with human PAF-AH is not due to variations in catalytic efficiency. A region located at the N-terminal end of PAF-AH regulates expression levels, the degree of hydrophobicity and polarity of the N-terminal region correlates with the level of PAF-AH expression
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LCAT_HUMAN
440
2
49578
Swiss-Prot
Secretory Pathway (Reliability: 1)
PAFA_HUMAN
441
0
50077
Swiss-Prot
Secretory Pathway (Reliability: 1)
LPP60_HUMAN
573
0
60883
Swiss-Prot
Mitochondrion (Reliability: 5)
PA1B2_HUMAN
229
0
25569
Swiss-Prot
other Location (Reliability: 1)
PA1B3_HUMAN
231
0
25734
Swiss-Prot
other Location (Reliability: 1)
PAFA2_HUMAN
392
0
44036
Swiss-Prot
other Location (Reliability: 2)
Q5SY01_HUMAN
165
0
18506
TrEMBL
other Location (Reliability: 2)
B2R9K4_HUMAN
392
0
44066
TrEMBL
other Location (Reliability: 2)
Q5SY00_HUMAN
185
0
21029
TrEMBL
other Location (Reliability: 2)
A8K2W6_HUMAN
441
0
50030
TrEMBL
Secretory Pathway (Reliability: 1)
A0A024RD39_HUMAN
441
0
50049
TrEMBL
Secretory Pathway (Reliability: 1)
B4DLM5_HUMAN
137
0
15568
TrEMBL
other Location (Reliability: 5)
B4DG88_HUMAN
219
0
24907
TrEMBL
other Location (Reliability: 3)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
-
x * 14000, secretory isozyme
17800
2 * 17800, SpV-1 forms higher molecular weight complexes ranging from 65000 Da to 110000 Da in addition to the 35000 Da complex calculated from sequence
22800
x * 22800, calculated from sequence
25000
29000
30000
31000
-
x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE
40000
-
1 * 45000, plasma isozyme, 1 * 40000, isozyme II
42700
-
1 * 42700, plasma enzyme, SDS-PAGE
43000 - 67000
-
plasma isozyme, gel filtration
43400
residues 47-429
45000
45390
-
sequence of cDNA
45400
-
-
70000
-
native enzyme, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
monomer
trimer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no modification
-
no glycoprotein, no sialic acid
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 1.5 A ligand-free crystal structure and a 2.1 A diethyl phosphoryl complex crystal structure of a 43.3 kDa construct, residues 47-429, of PAF acetylhydrolase are reported
data are collected to a resolution of 1.47 A
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A379V
-
nonsynonymous polymorphism
I198T
-
nonsynonymous polymorphism
I317N
Q281R
R92H
-
nonsynonymous polymorphism
V279F
V297F
-
common naturally occurring mutation in japanese population, inactive mutant enzyme, prevalence of the mutant allele is significantly higher in patients with asthma, stroke, myocardial infraction, brain hemorrhage, and nonfamilial cardiomyopathy
V379F
-
nonsynonymous polymorphism
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
complete inactivation
80944
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
30 min, stable up to, gradually decrease at temperatures above 40°C
70
-
30 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly sensitive to proteolysis
-
human PAF-AH protein is rapidly degraded after cycloheximide treatment of cells, a large portion of immunoreactive precursors are inactive and short-lived
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
catalytic activity of PAF-AH is sensitive to oxidants. Peroxynitrite mediates oxidative inactivation of PAF-AH. M117, and to a smaller contribution Y307 and Y335 are targets of oxidant attack. M117 is adjacent to W115 and L116, which are essential for association of PAF-AH with low-density lipoproteins. Oxidation of low-density lipoprotein-associated PAF-AH partially dissociates the enzyme from the particles. Oxidation of the purified enzyme in the absence of lipoproteins prevents subsequent association with low-density lipoproteins
-
679726
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from erythrocytes
-
from plasma
-
native enzyme 1400fold from erythrocytes by two different steps of anion exchange chromatography, followed by gel filtration, recombinant PAFAH1B2 or PAFAH1B3 subunits from HEK-293T cells
-
native enzyme from plasma
-
plasma isozyme to homogeneity, solubilization from LDL
-
recombinant protein using FLAG-tag
-
the erythrocyte isozyme from the soluble fraction of erythrocytes
-
using anti-FLAG affinity beads
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as FLAG-tag fusion protein in COS-7 cells, construction of transgenic mice
-
expressed in Xenopus laevis
-
expression in COS7 cells
-
expression in Escherichia coli
-
expression in Escherichia coli and in COS cells
-
expression in Escherichia coli BL-21
-
expression in Escherichia coli BL21 cells
-
expression of a fragment, corresponding to nucleotides 599-1123 of the human PAF-AH cDNA, quantitative realtime RT-PCR expression analysis in MM6 cells
-
expression of mutant proteins in COS-7 cells
-
expression of N- and C-FLAG-tagged wild-type mouse PAF-AH, expression of FLAG-tagged human wild-type PAF-AH and of the engineered PAF-AH mutant in Escherichia coli. A region located at the N-terminal end of PAF-AH regulates expression levels
-
expression of PAFAH1B2 or PAFAH1B3 subunits in HEK-293T cells
-
gene maps to chromosome 6q12.p21.1
-
overexpression of isozyme II in HaCaT cells, protects cells against apoptosis induced by tert-butylhydroperoxid, C2-ceramide or ultraviolet B radiation
-
plasma isozyme, DNA and amino acid sequence determination and analysis, gene maps at chromosome 6q12-21.1
-
residues 47-429, expression in Escherichia coli cells
transgenic mice overexpressing human PAF-AH II in neurons are generated. Human PAF-AH II expression is found throughout the central nervous system, especially in neurons of neocortex, hippocampus, and basal ganglia
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lipopolysaccharide and PAF strongly upregulate PAF-AH expression, the stimulation is susceptible to partial inhibition by p38 MAPK inhibitor SB-2033580 and PAF receptor antagonists WEB 2170 and BN50739, inhibition of the ERK1/2 portion of the MAPK pathway with PD98059 consistently increases PAF-AH expression relative to administration of lipopolysaccharide alone, overview. PAF-induced up-regulation of PAF-AH levels is solely mediated via the PAF receptor and is p38 MAPK-independent, lipopolysaccharide induce expression of the PAF receptor by 6-8fold
-
no effect by lyso-PAF on PAF-AH expression
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Stafforini, D.M.; Rollins, E.N.; Prescott, S.M.; McIntyre, T.M.
The platelet-activating factor acetylhydrolase from human erythrocytes. Purification and properties
J. Biol. Chem.
268
3857-3865
1993
Homo sapiens
Manually annotated by BRENDA team
Jarvi, K.; Langlais, J.; Gagnon, C.; Roberts, K.D.
Platelet-activating factor acetylhydrolase in the male reproductive tract: origin and properties
Int. J. Androl.
16
121-127
1993
Homo sapiens
Manually annotated by BRENDA team
Yamada, Y.; Stafforini, D.M.; Imaizumi, T.A.; Zimmermann, G.A.; McIntyre, T.M.; Prescott, S.M.
Characterization of the platelet-activating factor acetylhydrolase from human plasma by heterologous expression in Xenopus laevis oocytes
Proc. Natl. Acad. Sci. USA
91
10320-10324
1994
Homo sapiens
Manually annotated by BRENDA team
Tselepis, A.D.; Dentan, C.; Karabina, S.A.P.; Chapman, M.J.; Ninio, E.
PAF-degrading acetylhydrolase is preferentially associated with dense LDL and VHDL-1 in human plasma. Catalytic characteristics and relation to the monocyte-derived enzyme
Arterioscler. Thromb. Vasc. Biol.
15
1764-1773
1995
Homo sapiens
Manually annotated by BRENDA team
Tjoelker, L.W.; Wilder, C.; Eberhardt, C.; Stafforini, D.M.; Dietsch, G.; Schimpf, B.; Hooper, S.; Le Tong, H.; Cousens, L.S.; Zimmerman, G.A.; Yamada, Y.; McIntyre, T.M.; Prescott, S.M.; Gray, P.W.
Anti-inflammatory properties of a platelet-activating factor acetylhydrolase [see comments
Nature
374
549-553
1995
Homo sapiens
Manually annotated by BRENDA team
Triggiani, M.; De Marino, V.; Sofia, M.; Faraone, S.; Ambrosio, G.; Carratu, L.; Marone, G.
Characterization of platelet-activating factor acetylhydrolase in human bronchoalveolar lavage
Am. J. Respir. Crit. Care Med.
156
94-100
1997
Homo sapiens
Manually annotated by BRENDA team
Tew, D.G.; Boyd, H.F.; Ashman, S.A.; Theobald, C.; Leach, C.A.
Mechanism of inhibition of LDL phospholipase A2 by monocyclic-beta-lactams. Burst kinetics and the effect of stereochemistry
Biochemistry
1998
10087-10093
1998
Homo sapiens
-
Manually annotated by BRENDA team
Stafforini, D.M.; Prescott, S.M.; McIntyre, T.M.
Human plasma platelet-activating factor acetylhydrolase. Purification and properties
J. Biol. Chem.
262
4223-4230
1987
Homo sapiens
Manually annotated by BRENDA team
Moon, T.C.; Kim, M.S.; Lee, S.J.; Lee, T.Y.; Kwon, S.H.; Baek, S.H.; Chang, H.W.
Detection and characterization of 45 kDa platelet activating factor acetylhydrolase in cerebrospinal fluid of children with meningitis
Arch. Pharm. Res.
26
554-558
2003
Homo sapiens
Manually annotated by BRENDA team
Tsoukatos, D.C.; Liapikos, T.A.; Tselepis, A.D.; Chapman, M.J.; Ninio, E.
Platelet-activating factor acetylhydrolase and transacetylase activities in human plasma low-density lipoprotein
Biochem. J.
357
457-464
2001
Homo sapiens
Manually annotated by BRENDA team
Eisaf, M.; Tselepis, A.D.
Effect of hypolipidemic drugs on lipoprotein-associated platelet activating factor acetylhydrolase. Implication for atherosclerosis
Biochem. Pharmacol.
66
2069-2073
2003
Homo sapiens
Manually annotated by BRENDA team
Min, J.H.; Wilder, C.; Aoki, J.; Arai, H.; Inoue, K.; Paul, L.; Gelb, M.H.
Platelet-activating factor acetylhydrolases: broad substrate specificity and lipoprotein binding does not modulate the catalytic properties of the plasma enzyme
Biochemistry
40
4539-4549
2001
Homo sapiens, Physarum sp.
Manually annotated by BRENDA team
Tjoelker, L.W.; Stafforini, D.M.
Platelet-activating factor acetylhydrolases in health and disease
Biochim. Biophys. Acta
1488
102-123
2000
Oryctolagus cuniculus, Cricetinae, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Yasuda, K.; Okumura, T.; Okada, H.; Nakajima, T.; Aoki, J.; Arai, H.; Inoue, K.; Nishizawa, M.; Ito, S.; Kanzaki, H.
Platelet-activating factor acetylhydrolase isoforms I and II in human uterus. Comparisons with pregnant uterus and myoma
Biol. Reprod.
64
339-344
2001
Homo sapiens
Manually annotated by BRENDA team
Biancone, L.; Cantaluppi, V.; Del Sorbo, L.; Russo, S.; Tjoelker, L.W.; Camussi, G.
Platelet-activating factor inactivation by local expression of platelet-activating factor acetyl-hydrolase modifies tumor vascularization and growth
Clin. Cancer Res.
9
4214-4220
2003
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Caslake, M.J.; Packard, C.J.
Lipoprotein-associated phospholipase A2 (platelet-activating factor acetylhydrolase) and cardiovascular disease
Curr. Opin. Lipidol.
14
347-352
2003
Homo sapiens
Manually annotated by BRENDA team
Arai, H.; Koizumi, H.; Aoki, J.; Inoue, K.
Platelet-activating factor acetylhydrolase (PAF-AH)
J. Biochem.
131
635-640
2002
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Serban, M.; Tanaseanu, C.; Kosaka, T.; Vidulescu, C.; Stoian, I.; Marta, D.S.; Tanaseanu, S.; Moldoveanu, E.
Significance of platelet-activating factor acetylhydrolase in patients with non-insulin-dependent (type 2) diabetes mellitus
J. Cell. Mol. Med.
6
643-647
2002
Homo sapiens
Manually annotated by BRENDA team
Al-Darmaki, S.; Schenkein, H.A.; Tew, J.G.; Barbour, S.E.
Differential expression of platelet-activating factor acetylhydrolase in macrophages and monocyte-derived dendritic cells
J. Immunol.
170
167-173
2003
Homo sapiens
Manually annotated by BRENDA team
Marques, M.; Pei, Y.; Southall, M.D.; Johnston, J.M.; Arai, H.; Aoki, J.; Inoue, T.; Seltmann, H.; Zouboulis, C.C.; Travers, J.B.
Identification of platelet-activating factor acetylhydrolase II in human skin
J. Invest. Dermatol.
119
913-919
2002
Homo sapiens
Manually annotated by BRENDA team
Tsimihodimos, V.; Karabina, S.A.; Tambaki, A.P.; Bairaktari, E.; Miltiadous, G.; Goudevenos, J.A.; Cariolou, M.A.; Chapman, M.J.; Tselepis, A.D.; Elisaf, M.
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia
J. Lipid Res.
43
256-263
2002
Homo sapiens
Manually annotated by BRENDA team
Karasawa, K.; Harada, A.; Satoh, N.; Inoue, K.; Setaka, M.
Plasma platelet activating factor-acetylhydrolase (PAF-AH)
Prog. Lipid Res.
42
93-114
2003
Cavia porcellus, Homo sapiens
Manually annotated by BRENDA team
Sumita, C.; Maeda, M.; Fujio, Y.; Kim, J.; Fujitsu, J.; Kasayama, S.; Yamamoto, I.; Azuma, J.
Pioglitazone induces plasma platelet activating factor-acetylhydrolase and inhibits platelet activating factor-mediated cytoskeletal reorganization in macrophage
Biochim. Biophys. Acta
1673
115-121
2004
Homo sapiens
Manually annotated by BRENDA team
Nakos, G.; Kitsiouli, E.; Hatzidaki, E.; Koulouras, V.; Touqui, L.; Lekka, M.E.
Phospholipases A2 and platelet-activating-factor acetylhydrolase in patients with acute respiratory distress syndrome
Crit. Care Med.
33
772-779
2005
Homo sapiens
Manually annotated by BRENDA team
Stafforini, D.M.; Sheller, J.R.; Blackwell, T.S.; Sapirstein, A.; Yull, F.E.; McIntyre, T.M.; Bonventre, J.V.; Prescott, S.M.; Roberts, L.J.2nd.
Release of free F2-isoprostanes from esterified phospholipids is catalyzed by intracellular and plasma platelet-activating factor acetylhydrolases
J. Biol. Chem.
281
4616-4623
2006
Homo sapiens
Manually annotated by BRENDA team
Ishihara, M.; Iwasaki, T.; Nagano, M.; Ishii, J.; Takano, M.; Kujiraoka, T.; Tsuji, M.; Hattori, H.; Emi, M.
Functional impairment of two novel mutations detected in lipoprotein-associated phospholipase A2 (Lp-PLA2) deficiency patients
J. Hum. Genet.
49
302-307
2004
Homo sapiens
Manually annotated by BRENDA team
Vinson, S.M.; Rickard, A.; Ryerse, J.S.; McHowat, J.
Neutrophil adherence to bladder microvascular endothelial cells following platelet-activating factor acetylhydrolase inhibition
J. Pharmacol. Exp. Ther.
314
1241-1247
2005
Homo sapiens
Manually annotated by BRENDA team
Papavasiliou, E.C.; Gouva, C.; Siamopoulos, K.C.; Tselepis, A.D.
Erythrocyte PAF-acetylhydrolase activity in various stages of chronic kidney disease: effect of long-term therapy with erythropoietin
Kidney Int.
68
246-255
2005
Homo sapiens
Manually annotated by BRENDA team
Rizos, E.; Tambaki, A.P.; Gazi, I.; Tselepis, A.D.; Elisaf, M.
Lipoprotein-associated PAF-acetylhydrolase activity in subjects with the metabolic syndrome
Prostaglandins Leukot. Essent. Fatty Acids
72
203-209
2005
Homo sapiens
Manually annotated by BRENDA team
Quistad, G.B.; Fisher, K.J.; Owen, S.C.; Klintenberg, R.; Casida, J.E.
Platelet-activating factor acetylhydrolase: selective inhibition by potent n-alkyl methylphosphonofluoridates
Toxicol. Appl. Pharmacol.
205
149-156
2005
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Mitsios, J.V.; Vini, M.P.; Stengel, D.; Ninio, E.; Tselepis, A.D.
Human platelets secrete the plasma type of platelet-activating factor acetylhydrolase primarily associated with microparticles
Arterioscler. Thromb. Vasc. Biol.
26
1907-1913
2006
Homo sapiens
Manually annotated by BRENDA team
Karabina, S.; Ninio, E.
Plasma PAF-acetylhydrolase: An unfulfilled promise?
Biochim. Biophys. Acta
1761
1351-1358
2006
Homo sapiens
Manually annotated by BRENDA team
Karasawa, K.
Clinical aspects of plasma platelet-activating factor-acetylhydrolase
Biochim. Biophys. Acta
1761
1359-1372
2006
Homo sapiens
Manually annotated by BRENDA team
Seo, H.S.; Kim, J.H.; Nahm, M.H.
Platelet-activating factor-acetylhydrolase can monodeacylate and inactivate lipoteichoic acid
Clin. Vaccine Immunol.
13
452-458
2006
Homo sapiens
Manually annotated by BRENDA team
MacRitchie, A.N.; Gardner, A.A.; Prescott, S.M.; Stafforini, D.M.
Molecular basis for susceptibility of plasma platelet-activating factor acetylhydrolase to oxidative inactivation
FASEB J.
21
1164-1176
2007
Homo sapiens, Mus musculus (A0JNU3 and Q61206 and Q61205)
Manually annotated by BRENDA team
Zhu, J.; Massey, J.B.; Mitchell-Leef, D.; Elsner, C.W.; Kort, H.I.; Roudebush, W.E.
Platelet-activating factor acetylhydrolase activity affects sperm motility and serves as a decapacitation factor
Fertil. Steril.
85
391-394
2006
Homo sapiens
Manually annotated by BRENDA team
Davis, B.; Koster, G.; Douet, L.J.; Scigelova, M.; Woffendin, G.; Ward, J.M.; Smith, A.; Humphres, J.; Burnand, K.G.; Macphee, C.H.; Postle, A.D.
Electrospray ionization mass spectrometry identifies substrates and products of lipoprotein-associated phospholipase A2 in oxidized human low density lipoprotein
J. Biol. Chem.
283
6428-6437
2007
Homo sapiens
Manually annotated by BRENDA team
Chen, J.; Yang, L.; Foulks, J.M.; Weyrich, A.S.; Marathe, G.K.; McIntyre, T.M.
Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis
J. Lipid Res.
48
2365-2376
2007
Homo sapiens
Manually annotated by BRENDA team
Caini, P.; Guerra, C.T.; Giannini, C.; Giannelli, F.; Gragnani, L.; Petrarca, A.; Solazzo, V.; Monti, M.; Laffi, G.; Zignego, A.L.
Modifications of plasma platelet-activating factor (PAF)-acetylhydrolase/PAF system activity in patients with chronic hepatitis C virus infection
J. Viral Hepat.
14
22-28
2007
Homo sapiens
Manually annotated by BRENDA team
Scott, B.T.; Olson, N.; Long, G.L.; Bovill, E.G.
Novel isoforms of intracellular platelet activating factor acetylhydrolase (PAFAH1b2) in human testis; encoded by alternatively spliced mRNAs
Prostaglandins Other Lipid Mediat.
85
69-80
2007
Homo sapiens, Homo sapiens (P68402)
Manually annotated by BRENDA team
Umemura, K.; Kato, I.; Hirashima, Y.; Ishii, Y.; Inoue, T.; Aoki, J.; Kono, N.; Oya, T.; Hayashi, N.; Hamada, H.; Endo, S.; Oda, M.; Arai, H.; Kinouchi, H.; Hiraga, K.
Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse models of focal cerebral ischemia
Stroke
38
1063-1068
2007
Homo sapiens (Q99487), Homo sapiens
Manually annotated by BRENDA team
Lerman, A.; McConnell, J.P.
Lipoprotein-associated phospholipase A2: a risk marker or a risk factor?
Am. J. Cardiol.
101
11F-22F
2008
Homo sapiens
Manually annotated by BRENDA team
Anderson, J.L.
Lipoprotein-associated phospholipase A2: an independent predictor of coronary artery disease events in primary and secondary prevention
Am. J. Cardiol.
101
23F-33F
2008
Homo sapiens
Manually annotated by BRENDA team
Schmidt, E.B.; Koenig, W.; Khuseyinova, N.; Christensen, J.H.
Lipoprotein-associated phospholipase A2 concentrations in plasma are associated with the extent of coronary artery disease and correlate to adipose tissue levels of marine n-3 fatty acids
Atherosclerosis
196
420-424
2008
Homo sapiens
Manually annotated by BRENDA team
Rosenson, R.S.; Vracar-Grabar, M.; Helenowski, I.
Lipoprotein associated phospholipase A2 inhibition reduces generation of oxidized fatty acids: Lp-LPA2 reduces oxidized fatty acids
Cardiovasc. Drugs Ther.
22
55-58
2008
Homo sapiens
Manually annotated by BRENDA team
Stafforini, D.M.
Biology of platelet-activating factor acetylhydrolase (PAF-AH, lipoprotein associated phospholipase A2)
Cardiovasc. Drugs Ther.
23
73-83
2009
Homo sapiens
Manually annotated by BRENDA team
Brilakis, E.S.; Khera, A.; Saeed, B.; Banerjee, S.; McGuire, D.K.; Murphy, S.A.; de Lemos, J.A.
Association of lipoprotein-associated phospholipase A2 mass and activity with coronary and aortic atherosclerosis: findings from the Dallas Heart Study
Clin. Chem.
54
1975-1981
2008
Homo sapiens
Manually annotated by BRENDA team
Lavi, S.; Herrmann, J.; Lavi, R.; McConnell, J.P.; Lerman, L.O.; Lerman, A.
Role of lipoprotein-associated phospholipase A2 in atherosclerosis
Curr. Atheroscler. Rep.
10
230-235
2008
Homo sapiens
Manually annotated by BRENDA team
Gardner, A.A.; Reichert, E.C.; Topham, M.K.; Stafforini, D.M.
Identification of a domain that mediates association of platelet-activating factor acetylhydrolase with high density lipoprotein
J. Biol. Chem.
283
17099-17106
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Samanta, U.; Bahnson, B.J.
Crystal structure of human plasma platelet-activating factor acetylhydrolase: structural implication to lipoprotein binding and catalysis
J. Biol. Chem.
283
31617-31624
2008
Homo sapiens (Q13093), Homo sapiens
Manually annotated by BRENDA team
Tsoukatos, D.C.; Brocheriou, I.; Moussis, V.; Panopoulou, C.P.; Christofidou, E.D.; Koussissis, S.; Sismanidis, S.; Ninio, E.; Siminelakis, S.
Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery
J. Lipid Res.
49
2240-2249
2008
Homo sapiens
Manually annotated by BRENDA team
Gomes, M.d.e..B.; Cobas, R.A.; Nunes, E.; Nery, M.; Castro-Faria-Neto, H.C.; Tibirica, E.
Serum platelet-activating factor acetylhydrolase activity: a novel potential inflammatory marker in type 1 diabetes
Prostaglandins Other Lipid Mediat.
87
42-46
2008
Homo sapiens
Manually annotated by BRENDA team
Samanta, U.; Wilder, C.; Bahnson, B.J.
Crystallization and preliminary X-ray crystallographic analysis of human plasma platelet activating factor acetylhydrolase
Protein Pept. Lett.
16
97-100
2009
Homo sapiens
Manually annotated by BRENDA team
Gardner, A.A.; Reichert, E.C.; Alexander, T.S.; Topham, M.K.; Stafforini, D.M.
Novel mechanism for regulation of plasma platelet-activating factor acetylhydrolase expression in mammalian cells
Biochem. J.
428
269-279
2010
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Pande, A.H.; Tillu, V.A.
Membrane lipid composition differentially modulates the function of human plasma platelet activating factor-acetylhydrolase
Biochim. Biophys. Acta
1811
46-56
2011
Homo sapiens
Manually annotated by BRENDA team
Howard, K.M.; Abdel-Al, M.; Ditmyer, M.; Patel, N.
Lipopolysaccharide and platelet-activating factor stimulate expression of platelet-activating factor acetylhydrolase via distinct signaling pathways
Inflamm. Res.
60
735-744
2011
Homo sapiens
Manually annotated by BRENDA team
Zhou, G.; Marathe, G.K.; Willard, B.; McIntyre, T.M.
Intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood
J. Biol. Chem.
286
34820-34829
2011
Homo sapiens
Manually annotated by BRENDA team