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Information on EC 3.1.1.4 - phospholipase A2 and Organism(s) Rattus norvegicus

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2
IUBMB Comments
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
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Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a2, cpla2, spla2, spla(2), prdx6, cytosolic phospholipase a2, crotoxin, pla2s, ipla2, spla2-iia, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14 kDa phospholipase A2
-
-
-
-
Agkistrotoxin
-
-
-
-
aiPLA2
amdI1
-
-
-
-
Ammodytin I2
-
-
-
-
APLA
-
-
-
-
APP-D-49
-
-
-
-
ASPLA1
-
-
-
-
ASPLA10
-
-
-
-
ASPLA11
-
-
-
-
ASPLA12
-
-
-
-
ASPLA13
-
-
-
-
ASPLA14
-
-
-
-
ASPLA15
-
-
-
-
ASPLA16
-
-
-
-
ASPLA17
-
-
-
-
ASPLA2
-
-
-
-
ASPLA3
-
-
-
-
ASPLA4
-
-
-
-
ASPLA5
-
-
-
-
ASPLA6
-
-
-
-
ASPLA7
-
-
-
-
ASPLA8
-
-
-
-
ASPLA9
-
-
-
-
ATX
-
-
-
-
Basic protein I/II
-
-
-
-
BJ-PLA2
-
-
-
-
BJUPLA2
-
-
-
-
BPI/BPII
-
-
-
-
Ca2+-independent iPLA2
-
-
CaI-PLA2
-
-
-
-
calcium-d-independent cytosolic PLA2
-
-
calcium-dependent cytosolic PLA2
-
-
Caudoxin
-
-
-
-
cPm09
-
-
-
-
cytosolic cPLA2
-
-
cytosolic phospholipase A2alpha
-
-
Enhancing factor
-
-
-
-
GIIC sPLA2
-
-
-
-
GIID sPLA2
-
-
-
-
GIIE sPLA2
-
-
-
-
GIIF sPLA2
-
-
-
-
GIII sPLA2
-
-
-
-
Group IB phospholipase A2
-
-
-
-
Group IIA phospholipase A2
-
-
-
-
Group V phospholipase A2
-
-
-
-
Group VI phospholipase A2
-
-
-
-
GVI PLA2
-
-
-
-
GX sPLA2
-
-
-
-
GXII sPLA2
-
-
-
-
GXIII sPLA2
-
-
-
-
iPLA2
lecithinase A
-
-
-
-
MP-III 4R
-
-
-
-
Muscarinic inhibitor
-
-
-
-
Myotoxin
-
-
-
-
NAJPLA-2A
-
-
-
-
NAJPLA-2B
-
-
-
-
NAJPLA-2C
-
-
-
-
Nigexine
-
-
-
-
Non-pancreatic secretory phospholipase A2
-
-
-
-
Notechis 11'2
-
-
-
-
Notexin
-
-
-
-
NPLA
-
-
-
-
NPS-PLA2
-
-
-
-
OHV A-PLA2
-
-
-
-
OHV-APLA2
-
-
-
-
peroxiredoxin 6
pgPLA 1a/pgPLA 2a
-
-
-
-
phosphatidase
-
-
-
-
phosphatide 2-acylhydrolase
-
-
-
-
phosphatidolipase
-
-
-
-
Phosphatidylcholine 2-acylhydrolase
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIC
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIID
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIE
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIF
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIII
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GX
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GXII
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GXIII
-
-
-
-
phospholipase A
-
-
-
-
Phospholipase A2 inhibitor
-
-
-
-
pkP5
-
-
-
-
PLA2-10
-
-
-
-
PLA2-L
-
identical to cPLA2-alpha
PLA2-VI
-
-
-
-
PLA2-VII
-
-
-
-
PLA2IID
-
-
-
-
platelet activating factor acetyl hydrolase
-
-
-
-
Prdx6
Pt-PLA1
-
-
-
-
Pt-PLA2
-
-
-
-
secreted sPLA2
-
-
Secretory-type PLA, stroma-associated homolog
-
-
-
-
specific phospholipase A2
-
-
sPLA(2)-IID
-
-
-
-
sPLA(2)-IIE
-
-
-
-
sPLA(2)-IIF
-
-
-
-
sPLA2-IIE
-
-
TMV-K49
-
-
-
-
Toxin VI
-
-
-
-
Toxin VI:5
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 2-acylhydrolase
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-84-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dilinoleoyl-phosphatidylcholine + H2O
1-linoleoyl-glycerophosphorylcholine + linoleic acid
show the reaction diagram
-
-
-
-
?
1,2-dilinoleoyl-phosphatidylcholine + H2O
1-linoleoyl-sn-glycero-3-phosphocholine + linoleic acid
show the reaction diagram
-
-
-
-
?
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
show the reaction diagram
-
-
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + palmitic acid
show the reaction diagram
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
show the reaction diagram
-
-
-
-
?
1,2-dipalmitoyl-sn-phosphatidylcholine + H2O
1-palmitoyl-sn-phosphatidylcholine + palmitate
show the reaction diagram
the catalytic site for aiPLA2 activity is an S32-H26-D140 triad
-
-
?
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
1-palmitoyl-sn-phosphatidylserine + palmitic acid
show the reaction diagram
-
-
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
show the reaction diagram
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
show the reaction diagram
1-palmitoyl-2-arachidonyl-phosphatidylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphorylcholine + arachidonic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidic acid + H2O
1-palmitoyl-sn-glycero-3-phosphate + linoleic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine + H2O
1-palmitoyl-sn-glycerophosphoethanolamine + linoleic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylinositol + H2O
1-palmitoyl-3-phosphoinositol + linoleic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylserine + H2O
1-palmitoyl-sn-glycero-3-phosphoserine + linoleic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + linolic acid
show the reaction diagram
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
show the reaction diagram
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphocholine + oleic acid
show the reaction diagram
-
data for iPLA2
-
?
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + H2O
1-stearoyl-sn-glycero-3-phosphoinositol + arachidonic acid
show the reaction diagram
-
-
-
?
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
1-stearoyl-glycerophosphorylcholine + arachidonic acid
show the reaction diagram
arachidonyl phosphatidylethanolamine + H2O
glycerophosphoethanolamine + arachidonic acid
show the reaction diagram
-
-
-
-
?
diphosphatidylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
hen egg phosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
L-1-acyl-2-arachidonyl-sn-glycerol-3-phosphatidylethanolamine + H2O
?
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
1-acylglycerophosphocholine + fatty acid
show the reaction diagram
phosphatidylethanolamine + H2O
1-acylglycerophosphorylethanolamine + fatty acid
show the reaction diagram
phosphatidylglycerol + H2O
1-acylglycerol + fatty acid
show the reaction diagram
phospholipids + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phospholipids + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(6E)-6-(bromomethylene)-3-(1-naphthyl)tetrahydro-2H-pyran-2-one
-
i.e. BEL, bromoenol lactone suicide inhibitor, alkylates Cys-residues
1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol
-
MJ33, competitive inhibitor
3-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-(((2E)-4-[1-(diphenylmethyl)-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-7-(1H-1,2,4-triazol-1-yl)-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-fluoro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-methoxy-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-nitro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[7-chloro-3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-([(2E)-4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-en-1-yl]oxy)benzoate
-
-
4-([(2E)-4-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-yn-1-yl]oxy)benzoate
-
-
4-[2-[2-(1-benzyl-2-phenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]-2-hydroxybenzoate
-
-
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[2-(diphenylmethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[3-(2,2-diphenylethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-phenyl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]ethoxy]benzoic acid
-
-
4-[3-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]propoxy]benzoate
-
-
4-[4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]butoxy]benzoate
-
-
7,7-dimethyl-5,8-eicosadienoic acid
-
-
acteoside
-
phenylethanoid glycoside isolated from Clerodendron trichotomum Thunberg, competitive. Acts on cytosolic Ca2+-dependent phospholipase A2 rather than secretory phospholipase A2
anti-peptide antibody
-
group II PLA2-antibody
-
antiflammine
-
-
-
arachidonyl trifluoromethyl ketone
deoxycholate
-
substrate: phosphatidylcholine
diethyl 4-nitrophenyl phosphate
-
hexadecyl-3-trifluorethylglycero-sn-2-phospho-methanol
-
i.e. MJ33
KCl
-
strong inhibition
lysophosphatidylcholines
-
inhibitory plasma compounds, profile determination by mass spectroscopy, overview
methyl arachidonyl fluorophosphate
-
i.e. MAFP, an inhibitor of cysteine-dependent phospholipases
methyl-arachidonylfluorophosphonate
-
irreversible inhibitor of cPLA2 and iPLA2. Ser214 site in microtubule-associated protein Tau is hyperphosphorylated upon inhibitor treatment
NaCl
-
strong inhibition
palmitoyl lysophosphatidylcholines
-
competitive product inhibition
-
pure phospholipid vesicles
-
exception: vesicles composed of phosphatidylcholine or phosphatidylinositol 4,5-bisphosphate
-
stearoyl lysophosphatidylcholines
-
competitive product inhibition
-
surfactant protein A
-
-
-
thioetheramide
-
-
Triton X-100
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
-
a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity
Melittin
-
in vitro activator of secreted phospholipase A2
additional information
-
the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0175
1,2-dilinoleoyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.0082
1-palmitoyl-2-arachidonyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.0116
1-palmitoyl-2-linoleoyl-phosphatidic acid
-
pH 8.0, 25°C, recombinant enzyme
0.0186
1-palmitoyl-2-linoleoyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.005
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine
-
pH 8.0, 25°C, recombinant enzyme
0.0073
1-palmitoyl-2-linoleoyl-phosphatidylserine
-
pH 8.0, 25°C, recombinant enzyme
0.2
arachidonyl phosphatidylethanolamine
-
neutral nuclear PLA2
-
1.83
L-1-acyl-2-arachidonyl-sn-glycerol-3-phosphatidylethanolamine
-
pH 7.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0059
acteoside
-
pH 8.5, 22°C, crude protein preparation
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
3-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.012
4-(((2E)-4-[1-(diphenylmethyl)-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.014
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.011
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-7-(1H-1,2,4-triazol-1-yl)-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.006
4-(((2E)-4-[3-(diphenylmethyl)-7-fluoro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.009
4-(((2E)-4-[3-(diphenylmethyl)-7-methoxy-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.013
4-(((2E)-4-[3-(diphenylmethyl)-7-nitro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.011
4-(((2E)-4-[7-chloro-3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.05
4-([(2E)-4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-en-1-yl]oxy)benzoate
Rattus norvegicus
-
above
0.0013
4-([(2E)-4-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0016
4-([(2E)-4-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0013
4-([(2E)-4-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0024
4-([(2E)-4-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.023
4-([4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-yn-1-yl]oxy)benzoate
Rattus norvegicus
-
-
0.05
4-[2-[2-(1-benzyl-2-phenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.027
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]-2-hydroxybenzoate
Rattus norvegicus
-
-
0.0067
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
-
0.05
4-[2-[2-(diphenylmethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.03
4-[2-[3-(2,2-diphenylethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.05
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-phenyl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
above
0.005
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0075
4-[2-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0035
4-[2-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0056
4-[2-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.003
4-[2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0124
4-[3-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]propoxy]benzoate
Rattus norvegicus
-
-
0.05
4-[4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]butoxy]benzoate
Rattus norvegicus
-
above
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0172
-
substrate: 1-acyl-arachidonoyl-sn-glycero-3-phosphoethanolamine
0.04
-
renatured enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine
0.56
-
native enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine
0.6
-
iPLA2 in a 30000fold purification, substrate: 1-stearoyl-2-arachidonoylphosphatidylcholine
10
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-linoleoylphosphatidylcholine
2
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine
3
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-oleolylphosphatidylcholine
4.3
-
iPLA2 in a 30000fold purification, substrate: 1,2-dipalmitoyl-phosphatidylcholine
5.9
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
5000
-
substrate: phosphatidylethanolamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
lysosomal PLA
7.8
-
assay at
8
-
assay at
8.5 - 9
-
PLA in mitochondia, microsomes, plasma membrane
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
-
no activity below pH 7 or above pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
distribution of sPLa2-IIe, sPLA2-V, and sPLA2-X is mainly neuronal with highest abundance in cerebral cortex and hippocampus. Enzymes are differentially induced by kainic acid
Manually annotated by BRENDA team
-
isoform cPLA2-alpha
Manually annotated by BRENDA team
-
isoform cPLA2-alpha
Manually annotated by BRENDA team
-
isoform cPLA2-alpha
Manually annotated by BRENDA team
-
group IIC sPLA2
Manually annotated by BRENDA team
-
AdPLA is highly expressed specifically in white adipose tissue and is induced during preadipocyte differentiation into adipocytes, overview
Manually annotated by BRENDA team
additional information
-
expression profiles of isozymes iPLA2beta, sPLA2alpha, and AdPLA, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
in chromatin rich fraction
Manually annotated by BRENDA team
additional information
-
subcellular localization of AdPLA, overview
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PA24A_RAT
752
0
85707
Swiss-Prot
other Location (Reliability: 2)
PRDX6_RAT
224
0
24819
Swiss-Prot
other Location (Reliability: 1)
PA2G5_RAT
137
0
15996
Swiss-Prot
Secretory Pathway (Reliability: 1)
PA2GA_RAT
146
1
16294
Swiss-Prot
Secretory Pathway (Reliability: 1)
PA2GC_RAT
150
1
16919
Swiss-Prot
Secretory Pathway (Reliability: 2)
PA21B_RAT
146
0
16424
Swiss-Prot
Secretory Pathway (Reliability: 2)
PA2GX_RAT
151
0
17088
Swiss-Prot
Secretory Pathway (Reliability: 2)
PLAT3_RAT
160
1
17749
Swiss-Prot
other Location (Reliability: 2)
PLAT5_RAT
287
0
31749
Swiss-Prot
Mitochondrion (Reliability: 3)
PLPL2_RAT
478
0
52567
Swiss-Prot
Secretory Pathway (Reliability: 5)
PLAT1_RAT
167
0
18521
Swiss-Prot
other Location (Reliability: 2)
PLB1_RAT
1450
1
161089
Swiss-Prot
Secretory Pathway (Reliability: 1)
PAG15_RAT
413
1
47391
Swiss-Prot
Secretory Pathway (Reliability: 3)
PLPL9_RAT
807
0
89556
Swiss-Prot
Mitochondrion (Reliability: 4)
A0A8I6GKG0_RAT
1396
1
155749
TrEMBL
other Location (Reliability: 4)
A0A8I6AW05_RAT
145
0
16517
TrEMBL
Mitochondrion (Reliability: 2)
Q6IRF5_RAT
752
0
85649
TrEMBL
other Location (Reliability: 2)
F1M7W5_RAT
1111
0
122945
TrEMBL
Secretory Pathway (Reliability: 1)
F7F420_RAT
152
1
17141
TrEMBL
Secretory Pathway (Reliability: 3)
D3Z9B7_RAT
860
0
97689
TrEMBL
Secretory Pathway (Reliability: 3)
D3ZLB5_RAT
210
1
23450
TrEMBL
other Location (Reliability: 3)
A0A8I6ANS2_RAT
828
0
95516
TrEMBL
other Location (Reliability: 2)
A0A8I6AFX4_RAT
776
0
88256
TrEMBL
Mitochondrion (Reliability: 1)
B6VNU2_RAT
46
0
5331
TrEMBL
other Location (Reliability: 2)
A0A8I6G4E1_RAT
143
0
16112
TrEMBL
Secretory Pathway (Reliability: 2)
D3ZQH6_RAT
825
0
92923
TrEMBL
other Location (Reliability: 4)
B6VNU1_RAT
46
0
5347
TrEMBL
other Location (Reliability: 2)
A0A8I6ASV9_RAT
152
0
17048
TrEMBL
Mitochondrion (Reliability: 4)
A0A8I6A4T3_RAT
142
0
16336
TrEMBL
Secretory Pathway (Reliability: 4)
A0A8I6AKA4_RAT
851
0
98098
TrEMBL
other Location (Reliability: 5)
Q6DQ95_RAT
151
0
16925
TrEMBL
Secretory Pathway (Reliability: 2)
F1LPY6_RAT
874
0
100169
TrEMBL
other Location (Reliability: 2)
A0A8I6ALE8_RAT
140
0
15969
TrEMBL
Mitochondrion (Reliability: 4)
Q5BK35_RAT
144
0
16268
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I5ZNU3_RAT
744
0
84695
TrEMBL
other Location (Reliability: 2)
A0A8I6A5J8_RAT
133
0
15511
TrEMBL
other Location (Reliability: 3)
Q4V8L7_RAT
158
1
17722
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0G2KAA9_RAT
750
0
85436
TrEMBL
other Location (Reliability: 2)
F7EZZ6_RAT
785
0
89171
TrEMBL
Mitochondrion (Reliability: 5)
G3V6E2_RAT
151
0
17040
TrEMBL
Secretory Pathway (Reliability: 2)
D4A1I6_RAT
791
0
89555
TrEMBL
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
isoform PLA2-L
14000
-
secretory PLA2
15000
-
gel filtration, gel electrophoresis, SDS-PAGE
197000
-
gel filtration
200000
-
SDS-PAGE
200000 - 500000
-
isoform PLA2-H
80000
-
SDS-PAGE
85000 - 88000
-
-
90000
-
gel filtration, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 200000, SDS-PAGE
additional information
-
AdPLA contains no characteristic serine lipase motif, i.e. GXSXG or GXSGS
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C651A
-
catalytic activity similar to wild-type, inactivation by (6E)-6-(bromomethylene)-3-(1-naphthyl)tetrahydro-2H-pyran-2-one requires higher concentrations than for wild-type
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
for 10 min at pH 5.0 no loss of activity, at pH 7.0 50% of activity remains, pH 9.0 leads to complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, activity increased 3fold after storage for one week
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
30000fold from rat brain, 90% pure protein
-
column chromatography
-
column chromatography steps
-
column chromatography, immunoadsorbent column containing group II PLA2-antibodies, 735fold
-
recombinant untagged enzyme by ion-exchange chromatography and gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of untagged enzyme
-
overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells
-
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, H-C.; Mosior, M.; Ni, B.; Dennis, E.A.
Regional distribution, ontogeny, purification, and characterization of the Ca2+-independent phospholipase A2 from rat brain
J. Neurochem.
73
1278-1287
1999
Rattus norvegicus
Manually annotated by BRENDA team
Larsson Forsell, P.K.A.; Kennedy, B.P.; Claesson, H.E.
The human calcium-independent phospholipase A2 gene
Eur. J. Biochem.
262
575-585
1999
Rattus norvegicus, Homo sapiens (O60733), Homo sapiens
Manually annotated by BRENDA team
Chen, D.; Dennis, E.A.
Expression and characterization of human group V phospholipase A2
Biochim. Biophys. Acta
1394
57-64
1998
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Oishi, T.; Tamiya-Koizumi, K.; Kudo, I.; Iino, S.; Takagi, K.; Yoshida, Y.
Purification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells
FEBS Lett.
394
55-60
1996
Rattus norvegicus
Manually annotated by BRENDA team
Wang, R.; Dodia, C.R.; Jain, M.K.; Fisher, A.B.
Purification and characterization of a calcium-independent acidic phospholipase A2 from rat lung
Biochem. J.
304
131-137
1994
Rattus norvegicus
Manually annotated by BRENDA team
Nakatani, Y.; Hara, S.; Murakami, M.; Kudo, I.; Inoue, K.
Characterization of cytosolic phospholipase A2 in rat mastocystoma RBL-2H3
Biol. Pharm. Bull.
17
47-50
1994
Rattus norvegicus
Manually annotated by BRENDA team
Vignais, P.M.; Colbeau, A.; Ngo-Tri, H.; Pilarska, M.
Characterization and subcellular localization of lipase and phospholipase A activities in rat liver
Lipids
1
117-126
1976
Rattus norvegicus
-
Manually annotated by BRENDA team
Rahman, Y.E.; Cerny, E.A.; Peraino, C.
Purification and some properties of rat spleen phospholipase A
Biochim. Biophys. Acta
321
526-535
1973
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Hanahan, D.J.
Phospholipases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
71-85
1971
Crotalus adamanteus, Crotalus atrox, Rattus norvegicus, Sus scrofa
-
Manually annotated by BRENDA team
Winstead, M.V.; Balsinde, J.; Dennis, E.A.
Calcium-independent phospholipase A2: structure and function
Biochim. Biophys. Acta
1488
28-39
2000
Homo sapiens, Mammalia, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Lee, D.; Won, J.H.; Auh, C.K.; Park, Y.M.
Purification and characterization of a cytosolic phospholipase A2 from rat liver
Mol. Cells
16
361-367
2003
Rattus norvegicus
Manually annotated by BRENDA team
Farooqui, A.A.; Ong, W.Y.; Horrocks, L.A.; Farooqui, T.
Brain cytosolic phospholipase A2: localization, role, and involvement in neurological diseases
Neuroscientist
6
169-180
2000
Bos taurus, Homo sapiens, Mammalia, Rattus norvegicus
-
Manually annotated by BRENDA team
Song, H.; Ramanadham, S.; Bao, S.; Hsu, F.F.; Turk, J.
A bromoenol lactone suicide substrate inactivates group VIA phospholipase A2 by generating a diffusible bromomethyl keto acid that alkylates cysteine thiols
Biochemistry
45
1061-1073
2006
Rattus norvegicus
Manually annotated by BRENDA team
Kolko, M.; Christoffersen, N.R.; Barreiro, S.G.; Miller, M.L.; Pizza, A.J.; Bazan, N.G.
Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain
J. Neurosci. Res.
83
874-882
2006
Rattus norvegicus
Manually annotated by BRENDA team
Gopalsamy, A.; Yang, H.; Ellingboe, J.W.; McKew, J.C.; Tam, S.; Joseph-McCarthy, D.; Zhang, W.; Shen, M.; Clark, J.D.
1,2,4-Oxadiazolidin-3,5-diones and 1,3,5-triazin-2,4,6-triones as cytosolic phospholipase A2alpha inhibitors
Bioorg. Med. Chem. Lett.
16
2978-2981
2006
Rattus norvegicus
Manually annotated by BRENDA team
Wu, Y.; Feinstein, S.I.; Manevich, Y.; Chowdhury, I.; Pak, J.H.; Kazi, A.; Dodia, C.; Speicher, D.W.; Fisher, A.B.
Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity
Biochem. J.
419
669-679
2009
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Burke, J.E.; Dennis, E.A.
Phospholipase A2 biochemistry
Cardiovasc. Drugs Ther.
23
49-59
2009
Apis mellifera, Bitis gabonica, Bos taurus, Crotalus sp., Homo sapiens, Mus musculus, Naja naja, Oryza sativa, Rattus norvegicus, Sus scrofa, Protoparvovirus
Manually annotated by BRENDA team
Duncan, R.E.; Sarkadi-Nagy, E.; Jaworski, K.; Ahmadian, M.; Sul, H.S.
Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)
J. Biol. Chem.
283
25428-25436
2008
Rattus norvegicus
Manually annotated by BRENDA team
Cunningham, T.J.; Yao, L.; Lucena, A.
Product inhibition of secreted phospholipase A2 may explain lysophosphatidylcholines unexpected therapeutic properties
J. Inflamm.
5
17
2008
Rattus norvegicus
Manually annotated by BRENDA team
Song, C.; Zhang, X.Y.; Manku, M.
Increased phospholipase A2 activity and inflammatory response but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression: effects of chronic ethyl-eicosapentaenoate treatment
J. Neurosci.
29
14-22
2009
Rattus norvegicus
Manually annotated by BRENDA team
Kirincich, S.J.; Xiang, J.; Green, N.; Tam, S.; Yang, H.Y.; Shim, J.; Shen, M.W.; Clark, J.D.; McKew, J.C.
Benzhydrylquinazolinediones: novel cytosolic phospholipase A2alpha inhibitors with improved physicochemical properties
Bioorg. Med. Chem.
17
4383-4405
2009
Rattus norvegicus, synthetic construct
Manually annotated by BRENDA team
De-Paula, V.J.; Schaeffer, E.L.; Talib, L.L.; Gattaz, W.F.; Forlenza, O.V.
Inhibition of phospholipase A2 increases Tau phosphorylation at Ser214 in embryonic rat hippocampal neurons
Prostaglandins Leukot. Essent. Fatty Acids
82
57-60
2010
Rattus norvegicus
Manually annotated by BRENDA team
Du, Y.R.; Xiao, Y.; Lu, Z.M.; Ding, J.; Xie, F.; Fu, H.; Wang, Y.; Strong, J.A.; Zhang, J.M.; Chen, J.
Melittin activates TRPV1 receptors in primary nociceptive sensory neurons via the phospholipase A2 cascade pathways
Biochem. Biophys. Res. Commun.
408
32-37
2011
Rattus norvegicus
Manually annotated by BRENDA team
Song, H.S.; Choi, M.Y.; Ko, M.S.; Jeong, J.M.; Kim, Y.H.; Jang, B.H.; Sung, J.H.; Kim, M.G.; Whang, W.K.; Sim, S.S.
Competitive inhibition of cytosolic Ca2+-dependent phospholipase A2 by acteoside in RBL-2H3 cells
Arch. Pharm. Res.
35
905-910
2012
Rattus norvegicus
Manually annotated by BRENDA team
Fisher, A.B.
The phospholipase A2 activity of peroxiredoxin 6
J. Lipid Res.
59
1132-1147
2018
Trematomus bernacchii, Xenopus tropicalis (B1WAZ6), Mus musculus (O08709), Rattus norvegicus (O35244), Bos taurus (O77834), Homo sapiens (P30041), Gallus gallus (Q5ZJF4), Arabidopsis thaliana (Q8S8N6)
Manually annotated by BRENDA team