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Information on EC 3.1.1.31 - 6-phosphogluconolactonase and Organism(s) Plasmodium falciparum
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3.1.1.31 6-phosphogluconolactonaseSpecify your search results
The taxonomic range for the selected organisms is: Plasmodium falciparum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
6-phosphogluconolactonase, 6pgl, phosphogluconolactonase, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, g6pd-6pgl, 6-pgl, xoo2316, og1rf_11582, 6-phosphogluconolactonase 3, 6-phosphoglucono-gamma-lactonase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-PGL
-
-
-
-
6-phospho-D-glucose-delta-lactone hydrolase
-
-
-
-
6-phosphoglucono-gamma-lactonase
-
-
-
-
6PGL
-
-
-
-
glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
-
-
lactonase, phosphoglucono-
-
-
-
-
PGL
-
-
-
-
phosphogluconolactonase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate
PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
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-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-glucono-1,5-lactone lactonohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
37278-45-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-phospho-D-glucono-1,4-lactone + H2O
6-phospho-D-gluconate
-
the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring
-
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ellagic acid
mixed type inhibitor of glucose 6-phosphate activity
additional information
presence of H2O2 only slightly alters enzyme activity or expression
-
additional information
-
presence of H2O2 only slightly alters enzyme activity or expression
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
detailed kinetic analysis of the bifunctional glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase indicates that PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
46
-
recombinant PfGluPho, substrate is 6-phosphoglucono-gamma-lactone, pH 7.0, 25°C
60
60
-
recombinant PfGluPho, substrate is 6-phosphoglucono-delta-lactone, pH 7.0, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional enzyme having both glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase activity
SwissProt
bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase
UniProt
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
in Plasmodium falciparum, glucose-6-phosphate dehydrogenase is combined with the second enzyme of the pentose phosphate pathway to create a unique bifunctional enzyme named GluPho, glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase. The G6PD activity is not largely influenced by the 6PGL part
physiological function
the enzyme is essential for the growth of blood stage parasites
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q27741_PLAFA
910
0
106987
TrEMBL
Chloroplast (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15
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expression of lactonase domain Pf6PGL in Escherichia coli, low recovery of soluble Pf6PGL
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
no significant upregulation of expression in presence of artemisinin, chloroquine or methylene blue
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miclet, E.; Stoven, V.; Michels, P.A.M.; Opperdoes, F.R.; Lallemand, J.Y.; Duffieux, F.
NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase
J. Biol. Chem.
276
34840-34846
2001
Homo sapiens (O95336), Homo sapiens, Plasmodium falciparum (Q27741), Plasmodium falciparum, Trypanosoma brucei (Q9GRG6), Trypanosoma brucei
Crooke, A.; Diez, A.; Mason, P.J.; Bautista, J.M.
Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
FEBS J.
273
1537-1546
2006
Plasmodium falciparum
Jortzik, E.; Mailu, B.M.; Preuss, J.; Fischer, M.; Bode, L.; Rahlfs, S.; Becker, K.
Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: a unique bifunctional enzyme from Plasmodium falciparum
Biochem. J.
436
641-650
2011
Plasmodium falciparum, Homo sapiens (O95336), Homo sapiens
Allen, S.M.; Lim, E.E.; Jortzik, E.; Preuss, J.; Chua, H.H.; MacRae, J.I.; Rahlfs, S.; Haeussler, K.; Downton, M.T.; McConville, M.J.; Becker, K.; Ralph, S.A.
Plasmodium falciparum glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase is a potential drug target
FEBS J.
282
3808-3823
2015
Plasmodium falciparum (Q8IKU0), Plasmodium falciparum
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