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Information on EC 3.1.1.31 - 6-phosphogluconolactonase and Organism(s) Arabidopsis thaliana

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.31 6-phosphogluconolactonase
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Arabidopsis thaliana
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
6-phosphogluconolactonase, 6pgl, phosphogluconolactonase, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, g6pd-6pgl, 6-pgl, 6-phosphoglucono-gamma-lactonase, xoo2316, og1rf_11582, 6-phosphogluconolactonase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-PGL
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6-phospho-D-glucose-delta-lactone hydrolase
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6-phosphoglucono-gamma-lactonase
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6-phosphogluconolactonase
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6-phosphogluconolactonase 3
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lactonase, phosphoglucono-
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PGL
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phosphogluconolactonase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-glucono-1,5-lactone lactonohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37278-45-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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co-localization with thioredoxin m-type, Trx_m2
Manually annotated by BRENDA team
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co-localization with thioredoxin m-type, Trx_m2
Manually annotated by BRENDA team
additional information
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PGL3 contains an N-terminal extension and a C-terminal peroxisome-targeting motif PTS1, subcellular localization of enzyme stages, overview. Besides redox-related and metabolic signals that influence dual-targeting of enzyme PGL3 in leaves, developmental and/or physiological state(s) play another role for subcellular enzyme localization in different plant parts, the metabolic state affects PGL3 targeting by absence/reduced stability of thioredoxin Trxm2
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
pgl3 mutation knocks out most of the 6PGL activity. Knockdown of PGL3 leads to a dramatic decrease in plant size, a significant increase in total glucose-6-phosphate dehydrogenase activity and a marked decrease in cellular redox potential. A wild-type PGL3-GFP transgene complements all phenotypes caused by the pgl3 mutation. Plastidic localization and enzymatic activity are required for PGL3 to complement the pgl3 mutant phenotypes. Pgl3 mutation exhibits constitutive pathogenesis-related gene expression and enhanced resistance to Pseudomonas syringae pv. maculicola ES4326 and Hyaloperonospora arabidopsidis Noco2. The pgl3 mutation activates NPR1- and SID2/EDS16/ICS1-dependent defense signaling
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
6PGL1_ARATH
268
0
29887
Swiss-Prot
other Location (Reliability: 2)
6PGL2_ARATH
259
0
28863
Swiss-Prot
other Location (Reliability: 3)
6PGL3_ARATH
325
0
35645
Swiss-Prot
Chloroplast (Reliability: 1)
6PGL4_ARATH
261
0
29244
Swiss-Prot
other Location (Reliability: 4)
6PGL5_ARATH
252
0
28476
Swiss-Prot
other Location (Reliability: 2)
A0A654G497_ARATH
325
0
35645
TrEMBL
Chloroplast (Reliability: 1)
A0A178UM73_ARATH
253
0
28563
TrEMBL
other Location (Reliability: 3)
A0A178WNF3_ARATH
268
0
29877
TrEMBL
other Location (Reliability: 2)
A0A5S9Y978_ARATH
325
0
35590
TrEMBL
Chloroplast (Reliability: 1)
A0A5S9UAD7_ARATH
268
0
29917
TrEMBL
other Location (Reliability: 2)
A0A7G2FG87_ARATH
252
0
28452
TrEMBL
other Location (Reliability: 3)
A0A5S9Y6W9_ARATH
252
0
28466
TrEMBL
other Location (Reliability: 2)
A0A178UCQ0_ARATH
325
0
35645
TrEMBL
Chloroplast (Reliability: 1)
A0A178VCR4_ARATH
259
0
28883
TrEMBL
other Location (Reliability: 3)
A0A5S9XJ83_ARATH
259
0
28863
TrEMBL
other Location (Reliability: 3)
A0A654EAD0_ARATH
268
0
29887
TrEMBL
other Location (Reliability: 2)
A0A178UFN1_ARATH
261
0
29244
TrEMBL
other Location (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D150A
displays pgl3 morphology, does not complement the pgl3 phenotypes
D150A/D231A/H233A
displays pgl3 morphology, does not complement the pgl3 phenotypes
D231A/H233A
displays pgl3 morphology, does not complement the pgl3 phenotypes
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene PGL3, presence of the C-terminal SKL motif interfered with chloroplast import of a PGL3_N-full-CFP fusion and with that of a PGL3_long medial-CFP fusion (with free N- and C-terminal ends) exclusively labeling peroxisomes, co-expression of thioredoxin Trxm2 with C-terminally truncated enzyme PGL3_N-short and _N-long versions accumulate in the cytosol of tobacco protoplasts and co-localization with Trxm2 in the cytosol and in the chloroplast stroma, recombinant expression of N-terminally CFP-tagged, green-fluorescent enzyme in Nicotiana benthamiana leaves
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PCR product cloned into the corresponding sites of the vector pGEX-4T-1 and then introduced into Escherichia coli. Constructs (35S:DELTANTpgl3-GFP and 35S:pgl3 DELTASKL-GFP) together with the 35S:PGL3-GFP construct transformed into the pgl3 background. Escherichia coli cells expressing GST-PGL3 and GST-pgl3 mutants
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xiong, Y.; DeFraia, C.; Williams, D.; Zhang, X.; Mou, Z.
Characterization of Arabidopsis 6-phosphogluconolactonase T-DNA insertion mutants reveals an essential role for the oxidative section of the plastidic pentose phosphate pathway in plant growth and development
Plant Cell Physiol.
50
1277-1291
2009
Arabidopsis thaliana (Q84WW2), Arabidopsis thaliana (Q8LEV7), Arabidopsis thaliana (Q8LG70), Arabidopsis thaliana (Q9FIN1), Arabidopsis thaliana (Q9LMX8)
Manually annotated by BRENDA team
Hoelscher, C.; Meyer, T.; Von Schaewen, A.
Dual-targeting of Arabidopsis 6-phosphogluconolactonase 3 (PGL3) to chloroplasts and peroxisomes involves interaction with Trx m2 in the cytosol
Mol. Plant
7
252-255
2014
Arabidopsis thaliana
Manually annotated by BRENDA team