Information on EC 3.1.1.11 - pectinesterase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.11
-
RECOMMENDED NAME
GeneOntology No.
pectinesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pectin + n H2O = n methanol + pectate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
homogalacturonan degradation
-
-
Metabolic pathways
-
-
pectin degradation II
-
-
pectin degradation III
-
-
Pentose and glucuronate interconversions
-
-
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
pectin pectylhydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-98-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Acrocylindrium sp.
-
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
i.e. onion
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
71
-
-
Manually annotated by BRENDA team
A-3
-
-
Manually annotated by BRENDA team
KBN616
-
-
Manually annotated by BRENDA team
LV 10, two pectin hydrolases: I and II
-
-
Manually annotated by BRENDA team
i.e. oat
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subspecies Brassica napus oleifera, winter oil-seed rape
-
-
Manually annotated by BRENDA team
i.e. cauliflower
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; different varieties
-
-
Manually annotated by BRENDA team
Castilleja indivisa
Indian paintbrush
-
-
Manually annotated by BRENDA team
cv. Nausica and Arancha, highest enzyme activity at the beginning of the season
-
-
Manually annotated by BRENDA team
several isozymes
-
-
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. mandarin
-
-
Manually annotated by BRENDA team
Citrus reticulata Citrus sinensis
-
-
-
Manually annotated by BRENDA team
Citrus sp.
-
-
-
Manually annotated by BRENDA team
Citrus x paradisi Marsh grapefruit
Marsh grapefruit
-
-
Manually annotated by BRENDA team
Clostridium multifermentans
-
-
-
Manually annotated by BRENDA team
cranberry
-
-
-
Manually annotated by BRENDA team
Cucumis sativa
Pepino Almerica
-
-
Manually annotated by BRENDA team
i.e. cucumber
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
dodder
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
var. Alister, Killer, and Oslo, high activity in hyperhydrated leaves
-
-
Manually annotated by BRENDA team
strain 3937
-
-
Manually annotated by BRENDA team
strain B374
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Diospyros sp.
persimmon
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Fragaria sp.
strawberry
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Fusarium roseum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. artichoke
-
-
Manually annotated by BRENDA team
cv. Indian Summer
-
-
Manually annotated by BRENDA team
var. Luchistaya, Prozrachnaya
-
-
Manually annotated by BRENDA team
i.e. barley
-
-
Manually annotated by BRENDA team
frgament
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cells cultured in the presence of PME from orange peel
-
-
Manually annotated by BRENDA team
Macrosporium cladosporioides
-
-
-
Manually annotated by BRENDA team
i.e. Malpighia punicifolia, two isozymes PME1 and PME2
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
Monilia fructicola
-
-
-
Manually annotated by BRENDA team
Musa domestica
cv. Canendish, banana
-
-
Manually annotated by BRENDA team
Oospora sp.
-
-
-
Manually annotated by BRENDA team
Ophiobolus graminis
-
-
-
Manually annotated by BRENDA team
Orobanche aegyptiaca
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pellicularia filamentosa
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. avocado
-
-
Manually annotated by BRENDA team
Physalospora sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sweet cherry
-
-
Manually annotated by BRENDA team
i.e. peach
-
-
Manually annotated by BRENDA team
Prunus sp.
i.e. plum
-
-
Manually annotated by BRENDA team
i.e. pear
-
-
Manually annotated by BRENDA team
i.e. radish
-
-
Manually annotated by BRENDA team
Ribes sp.
i.e. gooseberry
-
-
Manually annotated by BRENDA team
i.e. raspberry
-
-
Manually annotated by BRENDA team
Sclerotinia libertiana
-
-
-
Manually annotated by BRENDA team
i.e. rye
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cultivars Desiree, Montrose, and Pentland Dell
-
-
Manually annotated by BRENDA team
sugar cane weevil
UniProt
Manually annotated by BRENDA team
i.e. lilac
-
-
Manually annotated by BRENDA team
i.e. cacao
-
-
Manually annotated by BRENDA team
Torulopsis candida
-
-
-
Manually annotated by BRENDA team
QM9414
-
-
Manually annotated by BRENDA team
QM9414
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. wheat
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. grape
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
anhydrogalacturonate + H2O
?
show the reaction diagram
citrus pectin + H2O
methanol + citrus pectate
show the reaction diagram
citrus pectin + H2O
methanol + pectate
show the reaction diagram
cyano-acetate + H2O
?
show the reaction diagram
high methoxyl pectin + H2O
?
show the reaction diagram
-
-
-
-
?
homogalacturonan + H2O
?
show the reaction diagram
homogalacturonan + n H2O
?
show the reaction diagram
-
-
-
?
methyl pectate + H2O
?
show the reaction diagram
-
high molecular weight methyl pectate
-
-
?
methyl-esterfied oligogalacturonides + H2O
?
show the reaction diagram
-
C6- and C1-substituted. De-esterification proceeds via a specific pattern, depending on the degree of polymerization. Initially, a first methyl ester of the oligomer is hydrolysed, resulting in one free carboxyl group. Subsequently this first product is preferred as a substrate and is de-esterified for a second time. This product is then accumulated and hereafter de-esterified further to the final product. The saturated hexamer is an exception to this: three methyl esters are removed very rapidly instead of two methyl esters.
-
?
methylated oligogalacturonides + H2O
?
show the reaction diagram
-
-
-
-
?
pectin + H2O
?
show the reaction diagram
pectin + H2O
methanol + pectate
show the reaction diagram
sisal fibre + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
citrus pectin + H2O
methanol + pectate
show the reaction diagram
-
best substrate
-
-
?
pectin + H2O
?
show the reaction diagram
pectin + H2O
methanol + pectate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
aluminium
CaCl2
-
activates with increasing temperature to a maximal value
Co2+
-
351.2% activity at 1 mM
Cr3+
-
216.5% activity at 1 mM
Cu2+
-
104.5% activity at 1 mM
EDTA
-
229% activity at 1 mM
Fe3+
-
107.1% activity at 1 mM
Mn2+
-
369.9% activity at 1 mM
NH4Cl
-
activates
Ni2+
-
331.8% activity at 1 mM
Pb2+
-
139.2% activity at 1 mM
SDS
-
105.9% activity at 1 mM
ZnSO4
-
activates
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin
-
82% residual activity at 20 mg/ml
(NH4)2SO4
Acrocylindrium sp.
-
-
(NH4)Cl
Acrocylindrium sp.
-
-
beta-mercaptoethanol
-
96% residual activity at 1 mM
Ca2+
-
60 mM Ca2+ decreases enzyme activity at atmospheric pressure and 45-60C, but increases activity at elevated pressure up to 300 MPa
catechinhydrate
-
83% residual activity at 20 mg/ml
Co2+
-
52% residual activity at 100 mM
coumaric acid
D-galacturonate
-
slight
D-glucose
dense phase carbon dioxide
-
the maximum reduction of the residual activity of apple PME exposed to dense phase carbon dioxide is 94.57% at 55?C for 60 min, the residual activity of apple PME after dense phase carbon dioxide exhibits no reduction or reactivation for 4 weeks at 4C
-
EGTA
-
EGTA treatment reduces PME activity, but the addition of Ca2+ with EGTA reinstates the activity in response to heat shock
epigallocatechin gallate
gallic acid
gallocatechin gallate
glycerol
Hg(CH3COO)2
-
-
Iodine
iodoacetic acid
K+
-
73% residual activity at 100 mM
Lauryl sulfate
-
-
Li2SO4
-
-
Mg2+
-
86% residual activity at 100 mM
Mn2+
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