Information on EC 2.8.2.15 - steroid sulfotransferase and Organism(s) Homo sapiens

sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis

677143

-

?

3'-phosphoadenylyl sulfate + 3beta-hydroxytibolone

adenosine 3',5'-bisphosphate + 3beta-hydroxytibolone 3-O-sulfate

Homo sapiens

-

sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis

677143

-

?

3'-phosphoadenylyl sulfate + 4-androstene-3,17-dione

adenosine 3',5'-bisphosphate + 3-O-sulfo-3,5-androstadien-3-ol-17-one

Homo sapiens

Q06520

-

760621

-

?

3'-phosphoadenylyl sulfate + 4-nitrophenol

adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate

Homo sapiens

-

702138

-

?

3'-phosphoadenylyl sulfate + beta-estradiol

adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate

Homo sapiens

-

702138

-

?

3'-phosphoadenylyl sulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

702138

-

?

3'-phosphoadenylyl sulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate

3 entries

3'-phosphoadenylyl sulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + dehydroepiandrosterone O-sulfate

Homo sapiens

-

673061

-

?

3'-phosphoadenylyl sulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate

Homo sapiens

Q06520

-

738099, 738552

-

?

3'-phosphoadenylyl sulfate + desipramine

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

702138

-

?

3'-phosphoadenylyl sulfate + L-triiodothyronine

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

702138

-

?

3'-phosphoadenylyl sulfate + pregnenolone

adenosine 3',5'-bisphosphate + pregnenolone sulfate

Homo sapiens

Q06520

-

738099

-

?

3'-phosphoadenylyl sulfate + xanthurenic acid

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

702138

-

?

3'-phosphoadenylylsulfate + 4'-hydroxy-2',3,4-trichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4'-hydroxy-2,3',4-trichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4-hydroxy-2',3,5-trichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4-hydroxy-2,4'-dichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4-hydroxy-3,3',5-trichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4-hydroxy-3,3'-dichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl

adenosine 3',5'-bisphosphate + ?

Homo sapiens

-

724741

-

?

3'-phosphoadenylylsulfate + androsterone

adenosine 3',5'-bisphosphate + 5alpha-androstane-17-one 3-sulfate

Homo sapiens

-

enzyme is an androsterone sulfotransferase as well as a dehydroepiandrosterone sulfotransferase, implying an important role in steroid homeostasis for the adrenals and liver

662232

-

?

3'-phosphoadenylylsulfate + cholesterol

adenosine 3',5'-bisphosphate + cholesterol 3-sulfate

2 entries

3'-phosphoadenylylsulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate

Homo sapiens

-

enzyme is an androsterone sulfotransferase as well as a dehydroepiandrosterone sulfotransferase, implying an important role in steroid homeostasis for the adrenals and liver

662232

-

?

3'-phosphoadenylylsulfate + dehydroepiandrosterone

adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate

5 entries

additional information

?

-

9 entries

Mg2+

2 entries

NaCl

Homo sapiens

-

activates

677143

2,3-dichlorophenol

Homo sapiens

-

IC50: 0.0007 mM, vs. 10 nM estradiol

662863

2,4-Dichlorophenol

Homo sapiens

-

IC50: 0.0007 mM, vs. 10 nM estradiol

662863

2,5-dichlorophenol

Homo sapiens

-

IC50: 0.0022 mM, vs. 10 nM estradiol

662863

2,6-Dichloro-4-nitrophenol

Homo sapiens

-

non-specific SULT inhibitor

702138

2,6-dichlorophenol

Homo sapiens

-

IC50: 0.0016 mM, vs. 10 nM estradiol

662863

2-chlorophenol

Homo sapiens

-

IC50: 0.02 mM, vs. 10 nM estradiol

662863

2-phenylphenol

Homo sapiens

-

IC50: 0.0052 mM, vs. 10 nM estradiol

662863

3',4'-dihydroxy-2,3-dichlorobiphenyl

Homo sapiens

-

724741

3',4'-dihydroxy-4-monochlorobiphenyl

Homo sapiens

-

724741

3,4-dichlorophenol

Homo sapiens

-

IC50: 0.013 mM, vs. 10 nM estradiol

662863

3,5-dichlorophenol

Homo sapiens

-

IC50: 0.017 mM, vs. 10 nM estradiol

662863

3-chlorophenol

Homo sapiens

-

IC50: 0.13 mM, vs. 10 nM estradiol

662863

4'-hydroxy-2',3,4-trichlorobiphenyl

Homo sapiens

-

724741

4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl

Homo sapiens

-

724741

4'-hydroxy-2,3',4-trichlorobiphenyl

Homo sapiens

-

724741

4'-hydroxy-2,3'-dichlorobiphenyl

Homo sapiens

-

724741

4'-hydroxy-2,5-dichlorobiphenyl

2 entries

4'-hydroxy-3,4-dichlorobiphenyl

Homo sapiens

-

724741

4'-hydroxy-4-monochlorobiphenyl

Homo sapiens

-

724741

4-chloro-3-methylphenol

Homo sapiens

-

IC50: 0.024 mM, vs. 10 nM estradiol

662863

4-Chlorophenol

Homo sapiens

-

IC50: 0.11 mM, vs. 10 nM estradiol

662863

4-hydroxy-2',3,5-trichlorobiphenyl

2 entries

4-hydroxy-2,4'-dichlorobiphenyl

Homo sapiens

-

724741

4-hydroxy-3,3',5-trichlorobiphenyl

Homo sapiens

-

724741

4-hydroxy-3,3'-dichlorobiphenyl

Homo sapiens

-

724741

4-hydroxy-3,5-dichlorobiphenyl

-

4-hydroxytamoxifen

noncompetitive inhibition

738099

5,7-dichlorokynurenic acid

Homo sapiens

-

702138

6'-hydroxy-3,3'-4-trichlorobiphenyl

Homo sapiens

-

724741

adenosine 3',5'-bisphosphate

Homo sapiens

-

645729

androsterone

Homo sapiens

-

stronger substrate inhibition than dehydroepiandrosterone adenosine

662232

celecoxib

Homo sapiens

-

heterotrophic modulator, 3-O-sulfation is inhibited by celecoxib to the same extent, independent of the concentration of 17alpha-ethynylestradiol, switches sulfation from the 3-O-position to the 17beta-O-position

661577

DCNP

Homo sapiens

-

677143

dehydroepiandrosterone

3 entries

endoxifen

Homo sapiens

Q06520

i.e. 4-hydroxy-N-desmethyltamoxifen, potent noncompetitive inhibition

738099

gavestinel

Homo sapiens

-

i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt

702138

Kynurenic acid

Homo sapiens

-

exhibits selective inhibitory effects on Sult1b1-mediated sulfation of various compounds with IC50 values in the low micromolar range

702138

L689,560

Homo sapiens

-

i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline

702138

L701,324

Homo sapiens

-

i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone

Mefenamic acid

-

N-demethyltamoxifen

competitive inhibition

738099

pregnenolone

Homo sapiens

-

substrate inhibition

645729

tamoxifen N-oxide

Homo sapiens

Q06520

noncompetitive inhibition

738099

additional information

2 entries

-

celecoxib

Homo sapiens

-

heterotrophic modulator, switches sulfation from the 3-O-position to the 17beta-O-position and activates such that the total sulfated product exceed product formation by the native enzyme, 3fold to 4fold at 0.25 mM celecoxib

661577

additional information

4 entries

-

0.0012

1-naphthol

Homo sapiens

-

isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

702138

0.191

17alpha-ethynylestradiol

Homo sapiens

-

661577

0.00001

17beta-estradiol

Homo sapiens

-

pH 7.4, 37°C

645734

0.0003 - 0.0096

3'-phosphoadenylylsulfate

5 entries

0.00079

3alpha-tibolone

Homo sapiens

-

pH 6.0, 37°C, recombinant SULT1A1

677143

0.0016 - 0.0185

3beta-hydroxyandrost-5-ene-17-one

6 entries

0.00056

3beta-tibolone

Homo sapiens

-

pH 7.0, 37°C, recombinant SULT1A1

677143

0.0262

4'-hydroxy-2',3,4-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0086

4'-hydroxy-2,3',4-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0081 - 0.046

4-hydroxy-2',3,5-trichlorobiphenyl

2 entries

0.0568

4-hydroxy-2,4'-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0136

4-hydroxy-3,3',5-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0366

4-hydroxy-3,3'-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0085

4-hydroxy-3,5-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0399

4-nitrophenol

Homo sapiens

-

isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

702138

0.0021

androsterone

Homo sapiens

-

pH 7.5, 37°C

662232

0.0006 - 0.025

dehydroepiandrosterone

6 entries

0.0000105

estrone

Homo sapiens

-

pH 7.4, 37°C

645734

0.145

L-triiodothyronine

Homo sapiens

-

isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

702138

0.0018 - 0.0072

pregnenolone

5 entries

0.0063 - 0.206

xanthurenic acid

2 entries

additional information

3 entries

-

0.13

androsterone

Homo sapiens

-

pH 7.5, 37°C

662232

0.1

dehydroepiandrosterone

Homo sapiens

-

pH 7.5, 37°C

662232

0.253

4-hydroxy-2',3,5-trichlorobiphenyl

Homo sapiens

-

pH 7.0, 37°C, recombinant SULT1A2

673061

0.0127 - 0.0194

4-hydroxytamoxifen

2 entries

0.0016

adenosine 3',5'-bisphosphate

Homo sapiens

-

pH 7.5

645729

0.0038

androsterone

Homo sapiens

-

pH 7.5, 37°C

662232

0.008 - 0.112

dehydroepiandrosterone

3 entries

0.0028 - 0.0035

endoxifen

2 entries

0.0098 - 0.0172

N-demethyltamoxifen

2 entries

0.0096 - 0.0169

tamoxifen N-oxide

2 entries

0.0007

2,3-dichlorophenol

Homo sapiens

-

IC50: 0.0007 mM, vs. 10 nM estradiol

662863

0.0007

2,4-Dichlorophenol

Homo sapiens

-

IC50: 0.0007 mM, vs. 10 nM estradiol

662863

0.0022

2,5-dichlorophenol

Homo sapiens

-

IC50: 0.0022 mM, vs. 10 nM estradiol

662863

0.0342 - 24

2,6-Dichloro-4-nitrophenol

2 entries

0.0016

2,6-dichlorophenol

Homo sapiens

-

IC50: 0.0016 mM, vs. 10 nM estradiol

662863

0.02

2-chlorophenol

Homo sapiens

-

IC50: 0.02 mM, vs. 10 nM estradiol

662863

0.0052

2-phenylphenol

Homo sapiens

-

IC50: 0.0052 mM, vs. 10 nM estradiol

662863

0.016

3',4'-dihydroxy-2,3-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.096

3',4'-dihydroxy-4-monochlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.013

3,4-dichlorophenol

Homo sapiens

-

IC50: 0.013 mM, vs. 10 nM estradiol

662863

0.017

3,5-dichlorophenol

Homo sapiens

-

IC50: 0.017 mM, vs. 10 nM estradiol

662863

0.13

3-chlorophenol

Homo sapiens

-

IC50: 0.13 mM, vs. 10 nM estradiol

662863

0.0025

4'-hydroxy-2',3,4-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0008

4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0044

4'-hydroxy-2,3',4-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0098

4'-hydroxy-2,3'-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0039 - 0.0075

4'-hydroxy-2,5-dichlorobiphenyl

2 entries

0.016

4'-hydroxy-3,4-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.028

4'-hydroxy-4-monochlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.024

4-chloro-3-methylphenol

Homo sapiens

-

IC50: 0.024 mM, vs. 10 nM estradiol

662863

0.11

4-Chlorophenol

Homo sapiens

-

IC50: 0.11 mM, vs. 10 nM estradiol

662863

0.0006

4-hydroxy-2',3,5-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.01

4-hydroxy-2,4'-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0044

4-hydroxy-3,3',5-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.014

4-hydroxy-3,3'-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.013

4-hydroxy-3,5-dichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.01 - 0.0167

4-hydroxytamoxifen

2 entries

0.1642 - 0.2536

5,7-dichlorokynurenic acid

2 entries

0.016

6'-hydroxy-3,3'-4-trichlorobiphenyl

Homo sapiens

-

in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37°C

724741

0.0017 - 0.0027

endoxifen

2 entries

0.0012 - 0.0017

gavestinel

2 entries

0.0337 - 9

Kynurenic acid

2 entries

0.0473 - 0.0536

L689,560

2 entries

0.005

Mefenamic acid

2 entries

0.0083 - 0.015

N-demethyltamoxifen

2 entries

0.0111 - 0.0161

tamoxifen N-oxide

0.0073

-

0.0175

-

purified recombinant SULT1A2, substrate 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl

0.0201

-

0.027

-

purified recombinant SULT1A2, substrate 4-hydroxy-2',3,5-trichlorobiphenyl

0.133

-

6 - 7

-

assay at

6.8

-

7

-

assay at

673061

7.5

Homo sapiens

-

assay at

673295

37

Homo sapiens

-

assay at

673061, 673295, 677143

-

aortic smooth muscle cell line CRL-1999

-

benign prostatic hyperplasia cell

-

-

-

breast adenocarcinoma cell

-

endometrial cell line

Homo sapiens

-

Ishikawa adenocarcinoma

645734

endometrial stromal cell

-

-

foetal tissue is a much richer source of enzyme than adult

-

-

673350, 675998, 676007, 725980, 738098

-

-

-

-

-

-

-

aortic smooth muscle cell line CRL-1999

prostate adenocarcinoma cell

Homo sapiens

O00204

-

738102

prostate gland epithelium

-

-

postmenopausal, SULT2A1

677143

SMMC-7721 cell

Homo sapiens

-

highest expression

-

-

-

additional information

endoplasmic reticulum

-

-

-

-

malfunction

-

knockdown of SULT2B1 in Hepa1-6 cells induces cell-cycle arrest and apoptosis and suppresses tumorigenesis in vivo

726332

metabolism

Homo sapiens

Q06520

enzyme SULT1E1 cannot catalyze the sulfation of either of these two DELTA4-3-ketosteroids, whereas SULT2B1b exhibits weak activity toward only 4-androstene-3,17-dione (7.1 pmol/min/mg enzyme), but not progesterone. Thus, SULT2A1 may be the only major human SULT that is capable of sulfating DELTA4-3-ketosteroids, particularly 4-androstene-3,17-dione

760621

physiological function

2 entries

additional information

Homo sapiens

Q06520

modeling of the binding of hSULT2A1 with substrate 4-androstene-3,17-dione using the crystal structure of 3-ketosteroid-DELTA4-(5alpha)-dehydrogenase from Rhodococcus jostii RHA1 in complex with 4-androstene-3,17-dione (PDB ID 4AT2) and two crystal structures of human SULT2A1 in complex with DHEA and lithocholic acid (PDB IDs 1J99 and 3F3Y) as templates. The intact steroid nucleus is important for the binding of DELTA4-3-keto steroids by SULT2A1. Proposed mechanism of DELTA4-3-ketosteroid sulfation by SULT2A1, overview. Enzyme residues W77 and H99 are likely involved in the catalysis, possibly in the deprotonation of C-6. The sulfation of 4-androstene-3,17-dione might progress through the formation of a hydroxyl group which is a typical target for sulfation, it would require the isomerization of 4-androstene-3,17-dione from a keto form to an enol form

760621

O00204 pBLAST

ST2B1_HUMAN

Homo sapiens

365

0

41308

Swiss-Prot

Show Sequence

Mitochondrion (Reliability: 1)

Q06520 pBLAST

ST2A1_HUMAN

Homo sapiens

285

0

33780

Swiss-Prot

Show Sequence

-

33000

Homo sapiens

-

immunoblot analysis, antibodies against rat liver sulfotransferases

645731

33785

Homo sapiens

-

x * 33785, calculated from amino acid sequence

724741

34000

2 entries

34500

Homo sapiens

-

2 * 34500, SDS-PAGE

35000

39620

isoform SULT2B1a

41000

-

x * 41000, SDS-PAGE

41300

isoform SULT2B1b

68000

-

sucrose density gradient ultracentrifugation

645723, 645724

68000 - 70000

Homo sapiens

-

gel filtration

645729

?

3 entries

dimer

5 entries

additional information

Homo sapiens

-

isozyme SULT2B1b has an extension at the proline- and serine-rich carboxyl end of about 53 amino acids

673295

phosphoprotein

2 entries

hanging drop vapor diffusion technique, structure of human dehydroepiandrosterone adenosine sulfotransferase in complex with androsterone has been solved at 2.7 A resolution

Homo sapiens

-

662232

A679G

Homo sapiens

-

naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview

676256

G187C

Homo sapiens

-

naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview

676256

G781A

Homo sapiens

-

naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview

676256

H99A

Homo sapiens

Q06520

site-directed mutagenesis, 50% reduced sulfating activity toward 4-androstene-3,17-dione compared to wild-type

760621

H99S

Homo sapiens

Q06520

site-directed mutagenesis, mutant H99S shows complete loss of the sulfating activity toward 4-androstene-3,17-dione

760621

W77A

Homo sapiens

Q06520

site-directed mutagenesis, 50% reduced sulfating activity toward 4-androstene-3,17-dione compared to wild-type

760621

Y160F

Homo sapiens

Q06520

site-directed mutagenesis, unaltered sulfating activity toward 4-androstene-3,17-dione compared to wild-type

760621

Y231F

Homo sapiens

Q06520

site-directed mutagenesis, unaltered sulfating activity toward 4-androstene-3,17-dione compared to wild-type

760621

additional information

3 entries

42

Homo sapiens

-

after 30 min, dehydroepiandrosterone sulfation activity with recombinant truncated SULT2B1b is decreased 3.5fold more than that of recombinant full-length SULT2B1b, after 45 min,the truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity

673295

42 - 45

Homo sapiens

-

truncated SULT2B1b is more sensitive to thermal inactivation than the full-length enzyme. After incubation at 42°C for 30 min, dehydroepiandrosterone sulfation activity with truncated SULT2B1b is decreased 3.5fold more than that of full-length enzyme. After 45 min of incubation at 42°C, truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity

673295

additional information

Homo sapiens

-

the proline- and serine-rich carboxyl end is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of SULT2B1b

673295

removal of the extension at the proline- and serine-rich carboxyl end of about 53 amino acids significantly decreases the thermostability of the expressed enzyme as well as decreasing the rate of dehydroepiandrosterone sulfation

Homo sapiens

-

673295

-70°C, stored in glass ampoules , 0.05 M Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 2% propylene glycol, 1 mg/ml protein, no significant difference in activity observed after storage for 3.5 months

Homo sapiens

-

645724

0°C, redialyzed againstTris/thiol buffer, stored under N2, activity retains for at least 4 weeks

Homo sapiens

-

645723

-

Homo sapiens

-

645723, 645724, 645729, 645731, 724741

DE-52 column chromatography and hydroxyapatite column chromatography

Homo sapiens

Q06520

738099

DE52 anion exchange column chromatography and hydroxyapatite column chromatography

Homo sapiens

-

724113

Ni-NTA agarose column chromatography

Homo sapiens

-

702138

Ni-NTA column chromatography and Protein A-Sepharose column chromatography

Homo sapiens

-

673295

recombinant enzyme, expressed in Escherichia coli

Homo sapiens

-

645738

recombinant His-tagged full-length SULT2B1b as well as truncated SULT2B1b without the PSC extension from Escherichia coli strain DH5alpha by nickel affinity chromatography

-

SULT2B1

-

baculovirus expression-system, expression in Sf9 cells

Homo sapiens

-

661577

cDNA for isozymes STS and EST cloned

Homo sapiens

-

645740

cloning of 5'-flanking region of SULT2A1 and expression of a SULT2A1-reporter construct in Caco-2 cells, transcriptional regulation mechanism analysis, co-expression of human nuclear receptors constitutive androstane receptor CAR and retinoid X receptor alpha RXRalpha which are involved in the regulation, electrophoretic mobility shift assay for interaction study, overview

Homo sapiens

-

677218

DHEA-ST cDNA cloned and sequenced from human adrenal cDNA library, expression vectors transfected into 293 cells, gene hEST, Hugo nomenclature named it STM gene, because it also codes for monoamine-sulfating phenolsulfotransferase M-PST

Homo sapiens

-

645733

enzyme expression induction by 1alpha,25-dihydroxyvitamin D3 via the vitamin D responsive element C/ERB-alpha, but not C/ERB-beta, coactivators p300, steroid receptor coactivator 1 and 2, SRC-1 and SRC-2, but not SRC-3, are also required, regulation of transcription and translation, overview, co-expression of SULT2A1 with C/ERB-alpha and 1alpha,25-dihydroxyvitamin D3 in murine NIH3T3 cells

Homo sapiens

-

675998

expressed in Escherichia coli BL21(DE3) cells

2 entries

expressed in livers from Rattus norvegicus and Mus musculus by using a recombinant adenovirus

Homo sapiens

-

723910

expression and expression analysis of wild-type and mutant SULT2A1

Homo sapiens

-

676256

expression of His-tagged full-length SULT2B1b and truncated SULT2B1b without the PSC extension in Escherichia coli strain DH5alpha and in human BeWo choriocarcinoma cells, translocation of the wild-type enzyme to nuclei, but not of the truncation mutant

Homo sapiens

-

673295

expression of SULT1A2 in Escherichia coli strain BL21 (DE3)

Homo sapiens

-

673061

expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b

Homo sapiens

-

675275

full-length isoform SULT2B1b as well as truncated SULT2B1b without the PS proline- and serine-rich carboxyl extension are expressed in Escherichia coli XL1-Blue cells

Homo sapiens

-

673295

gene SULT2A1, expression and regulation analysis, the enzyme promoter contains composite elements inducible by nuclear receptors pregnane X receptor, PXR, and constitutive androstane receptor, CAR, synergizing effects by HNF4alpha binding at a site near the promoter, overview

Homo sapiens

-

676007

gene SULT2A1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21

Homo sapiens

Q06520

760621

hEST1 and hEST2 cloned and overexpressed in Escherichia coli in fusion with GST

Homo sapiens

-

645738

SULT2A1 is a target gene for metabolic regulation/induction by thyroid hormone T3, T3 overexpressing Hep-G2 cells, with co-expression of the T3-receptor-alpha, show increased SULT2A1 expression in a DNA microarray analysis, 5fold at protein level and 9fold at mRNA level, which is inhibited by cycloheximide indicating an indirect regulation mechanism via the transcription factor steroidogenic factor 1, SF1, SULT2A1 contains seven SF1 binding sites, analysis of promoter activity in wild-type and truncation mutants of the enzyme, overview

Homo sapiens

-

673350

SULT2A1, determination of the promoter sequence, SULT2A1-reporter gene expression in Hep-G2 cells and expression analysis, the constitutive active receptor, CAR, mediates the methotrexate induction of SULT2A1 in both Caco-2 andHep G2 cells, vitamin D receptor, VDR, also upregulates SULT2A1 gene expression, while human pregnane X receptor, PXR, downregulates it

Homo sapiens

-

674394

SULT2B1 gene encodes 2 isoforms, SULT2B1a and SULT2B1b, generated by alternate splicing of the first exon, cloned and expressed in Escherichia coli

Homo sapiens

O00204, Q06520

645739

activation of liver X receptor alpha as well as liver X receptor synthetic ligands like TO901317 and GW3965 induce the expression of isoform SULT2A1 at mRNA, protein, and enzymatic levels

Homo sapiens

-

738098

down-regulation of RORalpha and/or RORgamma by small interfering RNA inhibits the expression of endogenous SULT2A1

Homo sapiens

-

725980

estrogen-related receptor alpha upregulates SULT2A1 transcription in Caco-2 cells

Homo sapiens

Q06520

738552

inhibition of SULT2B1b expression induces cell-cycle arrest and apoptosis in Hepa1-6 cells by upregulating the expression of FAS, downregulating the expression of cyclinB1, BCL2 and MYC in vitro and in vivo at both the transcript and protein levels. Knock-down of SULT2B1b expression significantly suppresses tumor growth in nude mouse xenografts

Homo sapiens

-

726332

retinoid-related orphan receptor (ROR)alpha and RORgamma transactivate the SULT2A1 gene promoter through their binding to a ROR response element found in the SULT2A1 gene promoter

Homo sapiens

-

725980

SULT2B1 is comparatively higher in the human hepatocarcinoma tumorous tissues than their adjacent tissues

Homo sapiens

-

726332

the amount of SULT2B1b mRNA in human endometrial tissues is significantly higher during the midluteal phase than during other phases of the menstrual cycle. Expression of SULT2B1b mRNA is induced by cAMP or phosphate in human endometrial stromal cells, whereas it is induced by cAMP or relaxin in endometrial epithelial cells

-

medicine

Adams, J.B.; McDonald, D.

Enzymic synthesis of steroid sulphates. XII. Isolation of dehydroepiandrosterone sulphotransferase from human adrenals by affinity chromatography

Biochim. Biophys. Acta

567

144-153

1979

Homo sapiens

156557

645724

Adams, J.B.; McDonald, D.

Enzymic synthesis of steroid sulphates. XIII. Isolation and properties of dehydroepiandrosterone sulphotransferase from human foetal adrenals

Biochim. Biophys. Acta

615

275-278

1980

Homo sapiens

6932974

645729

Falany, C.N.; Vasquez, M.E.; Kalb, J.M.

Purification and characterization of human liver dehydroepiandrosterone sulphotransferase

Biochem. J.

260

641-646

1989

Homo sapiens

2764897

645731

Forbes-Bamforth, K.J.; Coughtrie, M.W.H.

Identification of a new adult human liver sulfotransferase with specificity for endogenous and xenobiotic estrogens

Biochem. Biophys. Res. Commun.

198

707-711

1994

Homo sapiens

8297382

645733

Luu-The, V.; Bernier, F.; Dufort, I.

Steroid sulfotransferases

J. Endocrinol.

150

87-97

1996

Bos taurus, Cavia porcellus, Homo sapiens, Rattus norvegicus

8943792

645734

Chetrite, G.; Pasqualini, J.R.

Steroid sulphotransferase and 17beta-hydroxysteroid dehydrogenase activities in Ishikawa human endometrial adenocarcinoma cells

J. Steroid Biochem. Mol. Biol.

61

27-34

1997

Homo sapiens

9328207

645738

Faucher, F.; Lacoste, L.; Dufort, I.; Luu-The, V.

High metabolization of catecholestrogens by type 1 estrogen sulfotransferase (hEST1)

J. Steroid Biochem. Mol. Biol.

77

83-86

2001

Homo sapiens

11358677

645739

Meloche, C.A.; Falany, C.N.

Expression and characterization of the human 3b-hydroxysteroid sulfotransferases (SULT2B1alpha and SULT2B1beta)

J. Steroid Biochem. Mol. Biol.

77

261-269

2001

Homo sapiens, Homo sapiens (O00204), Homo sapiens (Q06520)

11457664

645740

Nakamura, Y.; Miki, Y.; Suzuki, T.; Nakata, T.; Darnel, A.D.; Moriya, T.; Tazawa, C.; Saito, H.; Ishibashi, T.; Takahashi, S.; Yamada, S.; Sasano, H.

Steroid sulfatase and estrogen sulfotransferase in the atherosclerotic human aorta

Am. J. Pathol.

163

1329-1339

2003

Homo sapiens

14507642

645741

Suzuki, T.; Nakata, T.; Miki, Y.; Kaneko, C.; Moriya, T.; Ishida, T.; Akinaga, S.; Hirakawa, H.; Kimura, M.; Sasano, H.

Estrogen sulfotransferase and steroid sulfatase in human breast carcinoma

Cancer Res.

63

2762-2770

2003

Homo sapiens

12782580

660966

He, D.; Meloche, C.A.; Dumas, N.A.; Frost, A.R.; Falany, C.N.

Different subcellular localization of sulfotransferase 2B1b in human placenta and prostate

Biochem. J.

379

533-540

2004

Homo sapiens

14741047

661253

He, D.; Frost, A.R.; Falany, C.N.

Identification and immunohistochemical localization of sulfotransferase 2B1b (SULT2B1b) in human lung

Biochim. Biophys. Acta

1724

119-126

2005

Homo sapiens

15878639

661577

Cui, D.; Booth-Genthe, C.L.; Carlini, E.; Carr, B.; Schrag, M.L.

Heterotropic modulation of sulfotransferase 2A1 activity by celecoxib: product ratio switching of ethynylestradiol sulfation

Drug Metab. Dispos.

32

1260-1264

2004

Homo sapiens

15483193

662232

Chang, H.J.; Shi, R.; Rehse, P.; Lin, S.X.

Identifying androsterone (ADT) as a cognate substrate for human dehydroepiandrosterone sulfotransferase (DHEA-ST) important for steroid homeostasis: structure of the enzyme-ADT complex

J. Biol. Chem.

279

2689-2696

2004

Homo sapiens

14573603

662582

Higashi, Y.; Fuda, H.; Yanai, H.; Lee, Y.; Fukushige, T.; Kanzaki, T.; Strott, C.A.

Expression of cholesterol sulfotransferase (SULT2B1b) in human skin and primary cultures of human epidermal keratinocytes

J. Invest. Dermatol.

122

1207-1213

2004

Homo sapiens

15140224

662863

Harris, R.M.; Kirk, C.J.; Waring, R.H.

Non-genomic effects of endocrine disrupters: inhibition of estrogen sulfotransferase by phenols and chlorinated phenols

Mol. Cell. Endocrinol.

244

72-74

2005

Homo sapiens

16266779

673061

Liu, Y.; Apak, T.I.; Lehmler, H.J.; Robertson, L.W.; Duffel, M.W.

Hydroxylated polychlorinated biphenyls are substrates and inhibitors of human hydroxysteroid sulfotransferase SULT2A1

Chem. Res. Toxicol.

19

1420-1425

2006

Homo sapiens

17112228

673295

He, D.; Falany, C.N.

Characterization of proline-serine-rich carboxyl terminus in human sulfotransferase 2B1b: immunogenicity, subcellular localization, kinetic properties, and phosphorylation

Drug Metab. Dispos.

34

1749-1755

2006

Homo sapiens

16855051

673350

Huang, Y.H.; Lee, C.Y.; Tai, P.J.; Yen, C.C.; Liao, C.Y.; Chen, W.J.; Liao, C.J.; Cheng, W.L.; Chen, R.N.; Wu, S.M.; Wang, C.S.; Lin, K.H.

Indirect regulation of human dehydroepiandrosterone sulfotransferase family 1A member 2 by thyroid hormones

Endocrinology

147

2481-2489

2006

Homo sapiens

16469813

674394

Chen, X.; Maiti, S.; Zhang, J.; Chen, G.

Nuclear receptor interactions in methotrexate induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1)

J. Biochem. Mol. Toxicol.

20

309-317

2006

Homo sapiens

17163485

675275

Fuda, H.; Javitt, N.B.; Mitamura, K.; Ikegawa, S.; Strott, C.A.

Oxysterols are substrates for cholesterol sulfotransferase

J. Lipid Res.

48

1343-1352

2007

Homo sapiens

17347498

675998

Song, C.S.; Echchgadda, I.; Seo, Y.; Oh, T.; Kim, S.; Kim, S.; Cho, S.; Shi, L.; Chatterjee, B.

An essential role of the CAAT/enhancer binding protein-alpha in the vitamin D-induced expression of the human steroid/bile acid-sulfotransferase (SULT2A1)

Mol. Endocrinol.

20

1286

2006

Homo sapiens

16357103

676007

Echchgadda, I.; Song, C.S.; Oh, T.; Ahmed, M.; De La Cruz, I.J.; Chatterjee, B.

The xenobiotic-sensing nuclear receptors pregnane X receptor, constitutive androstane receptor, and orphan nuclear receptor hepatocyte nuclear factor 4alpha in the regulation of human steroid-/bile acid-sulfotransferase

Mol. Endocrinol.

21

2099-2111

2007

Homo sapiens

17595319

676256

Nowell, S.; Falany, C.N.

Pharmacogenetics of human cytosolic sulfotransferases

Oncogene

25

1673-1678

2006

Homo sapiens

16550167

677143

Wang, M.; Ebmeier, C.C.; Olin, J.R.; Anderson, R.J.

Sulfation of tibolone metabolites by human postmenopausal liver and small intestinal sulfotransferases (SULTs)

Steroids

71

343-351

2006

Homo sapiens

16360722

677218

Chen, X.; Zhang, J.; Baker, S.M.; Chen, G.

Human constitutive androstane receptor mediated methotrexate induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1)

Toxicology

231

224-233

2007

Homo sapiens

17276571

702138

Senggunprai, L.; Yoshinari, K.; Yamazoe, Y.

Inhibitory effects of kynurenic acid, a tryptophan metabolite, and its derivatives on cytosolic sulfotransferases

Biochem. J.

422

455-462

2009

Homo sapiens, Mus musculus

19548878

723910

Zhang, X.; Bai, Q.; Xu, L.; Kakiyama, G.; Pandak, W.M.; Zhang, Z.; Ren, S.

Cytosolic sulfotransferase 2B1b promotes hepatocyte proliferation gene expression in vivo and in vitro

Am. J. Physiol. Gastrointest. Liver Physiol.

303

G344-G355

2012

Homo sapiens

22679001

724113

Gulcan, H.O.; Duffel, M.W.

Substrate inhibition in human hydroxysteroid sulfotransferase SULT2A1: studies on the formation of catalytically non-productive enzyme complexes

Arch. Biochem. Biophys.

507

232-240

2011

Homo sapiens

21187059

724741

Ekuase, E.J.; Liu, Y.; Lehmler, H.J.; Robertson, L.W.; Duffel, M.W.

Structure-activity relationships for hydroxylated polychlorinated biphenyls as inhibitors of the sulfation of dehydroepiandrosterone catalyzed by human hydroxysteroid sulfotransferase SULT2A1

Chem. Res. Toxicol.

24

1720-1728

2011

Homo sapiens

21913674

725045

Koizumi, M.

Momoeda, M.; Hiroi, H.; Hosokawa, Y.; Tsutsumi, R.; Osuga, Y.; Yano, T.; Taketani, Y.: Expression and regulation of cholesterol sulfotransferase (SULT2B1b) in human endometrium

Fertil. Steril.

93

1538-1544

2010

Homo sapiens

19243756

725980

Ou, Z.; Shi, X.; Gilroy, R.K.; Kirisci, L.; Romkes, M.; Lynch, C.; Wang, H.; Xu, M.; Jiang, M.; Ren, S.; Gramignoli, R.; Strom, S.C.; Huang, M.; Xie, W.

Regulation of the human hydroxysteroid sulfotransferase (SULT2A1) by RORalpha and RORgamma and its potential relevance to human liver diseases

Mol. Endocrinol.

27

106-115

2013

Homo sapiens

23211525

726332

Yang, X.; Xu, Y.; Guo, F.; Ning, Y.; Zhi, X.; Yin, L.; Li, X.

Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo

PLoS ONE

8

e60853

2013

Homo sapiens

23593328

738098

Ou, Z.; Jiang, M.; Hu, B.; Huang, Y.; Xu, M.; Ren, S.; Li, S.; Liu, S.; Xie, W.; Huang, M.

Transcriptional regulation of human hydroxysteroid sulfotransferase SULT2A1 by LXR?

Drug Metab. Dispos.

42

1684-1689

2014

Homo sapiens

25028566

738099

Squirewell, E.J.; Qin, X.; Duffel, M.W.

Endoxifen and other metabolites of tamoxifen inhibit human hydroxysteroid sulfotransferase 2A1 (hSULT2A1)

Drug Metab. Dispos.

42

1843-1850

2014

Homo sapiens (Q06520), Homo sapiens

25157097

738102

Falany, C.N.; Rohn-Glowacki, K.J.

SULT2B1: unique properties and characteristics of a hydroxysteroid sulfotransferase family

Drug Metab. Rev.

45

388-400

2013

Homo sapiens (O00204), Homo sapiens

24020383

738552

Huang, C.; Zhou, T.; Chen, Y.; Sun, T.; Zhang, S.; Chen, G.

Estrogen-related receptor ERRalpha regulation of human hydroxysteroid sulfotransferase (SULT2A1) gene expression in human Caco-2 cells

J. Biochem. Mol. Toxicol.

28

32-38

2014

Homo sapiens (Q06520)

24038886

760621

Hashiguchi, T.; Kurogi, K.; Shimohira, T.; Teramoto, T.; Liu, M.C.; Suiko, M.; Sakakibara, Y.

DELTA4-3-ketosteroids as a new class of substrates for the cytosolic sulfotransferases

Biochim. Biophys. Acta

1861

2883-2890

2017

Homo sapiens (Q06520)

28782626

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

MetaCyc

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)