Information on EC 2.8.1.3 - thiosulfate-thiol sulfurtransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.8.1.3
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
thiosulfate-thiol sulfurtransferase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
mechanism varies with the acceptor substrate employed
-
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
mechanism, substrates add in a random fashion
-
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
mechanism
-
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfur atom transfer
-
-
-
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SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
thiosulfate disproportionation I (thiol-dependent)
-
-
Glutathione metabolism
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SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
thiosulfate:thiol sulfurtransferase
The primary product is glutathione hydrodisulfide, which reacts with glutathione to give glutathione disulfide and sulfide. L-Cysteine can also act as acceptor.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glutathione-dependent thiosulfate reductase
-
-
-
-
sulfane reductase
-
-
-
-
sulfane sulfurtransferase
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-
-
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sulfurtransferase, sulfane
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-
-
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SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
111070-24-7
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SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thiosulfate + cyanide
?
show the reaction diagram
-
-
-
-
?
thiosulfate + dithiothreitol
?
show the reaction diagram
-
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
?
benzenethiosulfonate + glutathione
additional information
-
-
rapid equilibrium-ordered mechanism with glutathione as leading substrate
glutathione persulfide as an immediate product
?
L-cysteine + glutathione
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme has two distinct closely situated substrate binding sites, one for compounds with an RSO3-structure and one for the sulfhydryl substrate
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-
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SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + glutathione
sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
benzene sulfinate
-
competitive with both substrates
oxidized glutathione
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product inhibition, competitive with glutathione
sulfate
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non-competitive inhibition when glutathione is the varied substrate
Sulfide
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product inhibition, competitive versus both substrates
sulfite
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competitive with respect to dithiothreitol, non-competitive with respect to thiosulfate
sulfite
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product inhibition, competitive versus both substrates
sulfite
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competitive inhibition when glutathione is the varied substrate
benzene thiosulfonate
-
competitive with both substrates
additional information
-
alkylation of cysteine residues with iodoacetate or iodoacetamide does not inactivate
-
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.24
Benzenethiosulfonate
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pH 8.1, 37C
3
cyanide
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pH 8.5, 27C
0.89
glutathione
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pH 8.1, 37C, with benzenethiosulfonate as the other substrate
4
glutathione
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pH 8.1, 37C, with thiosulfate as the other substrate
2.9
thiosulfate
-
pH 8.1, 37C
3.2
thiosulfate
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pH 8.5, 27C
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
118
sulfate
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pH 8.5, 27C
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
-
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.1
-
isoelectric focusing using ampholytes pH 4-6 and pH 2-10
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
17000
-
SDS-PAGE, gel filtration
645570
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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1 * 17000, SDS-PAGE
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
extremely labile during all ion-exchange steps
-
freezing and thawing during the later stages of purification destabilize
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SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-35C, concentrated enzyme in solution of 50 mM Tris, pH 8.0, 0.5 mM NaS2O3, 50% glycerol, stable for at least 4 months
-
-40C, 50 mM Tris-acetate, pH 8.0, 0.5 mM Na2S2O3, 50% glycerol, stable for at least 9 months
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SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme loses catalytic activity in 6 M guanidinium hydrochloride, renaturation after a short period of time by dialysis is rapid and complete. Renaturation after 10 weeks results in only partial restoration of enzyme activity. Full renaturation can be obtained by adding thioglycolate or 2-mercaptoethanol during denaturation. Renaturation rate is temperature dependent
-
SplaateApplication,Organism,Commentary,Literature