Information on EC 2.7.8.7 - holo-[acyl-carrier-protein] synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.7
-
RECOMMENDED NAME
GeneOntology No.
holo-[acyl-carrier-protein] synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acyl carrier protein activation
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acyl carrier protein metabolism
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enterobactin biosynthesis
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Pantothenate and CoA biosynthesis
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lipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
CoA-[4'-phosphopantetheine]:apo-[acyl-carrier protein] 4'-pantetheinephosphotransferase
Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain [3]. The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty acid synthase and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes [6]. Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14, [acyl-carrier-protein] phosphodiesterase.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-30-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain FGSC4
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
isolate ND90Pr (ATCC 201652)
UniProt
Manually annotated by BRENDA team
strain ATCC MYA-2876
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain MP-1
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-
Manually annotated by BRENDA team
strain MP-1
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-
Manually annotated by BRENDA team
strain Agy99
UniProt
Manually annotated by BRENDA team
no activity in Escherichia coli
strain MP4, denoted acpS1 gene
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli MP4
strain MP4, denoted acpS1 gene
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-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
yeast, strain lys5
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
castor-oil-seed, L. cv. Baker 296
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-
Manually annotated by BRENDA team
strain ATCC24843
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-
Manually annotated by BRENDA team
strain ATCC24843
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-
Manually annotated by BRENDA team
Melody
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-
Manually annotated by BRENDA team
strain DW4/3-1, gene mtaA
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Streptomyces pneumoniae
isolated from Yunnan, China
UniProt
Manually annotated by BRENDA team
isolated from Yunnan, China
UniProt
Manually annotated by BRENDA team
strain ATCC15003, nucleotide sequence of the svp locus
SwissProt
Manually annotated by BRENDA team
strain ATCC15003, nucleotide sequence of the svp locus
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
acetonyldethio-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + acyl-carrier protein
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + apo-[acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + polyketide synthase 1
CoA + acetyl-polyketide synthase 1
show the reaction diagram
acetyl-CoA + polyketide synthase 16
CoA + acetyl-polyketide synthase 16
show the reaction diagram
acetyl-CoA + polyketide synthase 2
CoA + acetyl-polyketide synthase 2
show the reaction diagram
apo-[acyl-carrier protein] + acetyl-CoA
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
pH 7, 37°C
reaction stop by 10% trichloroacetic acid, limited release of 3’,5’-ADP by interactions with guanidinium moieties of R74 and R86
-
?
benzoyl-CoA + apo-[acyl-carrier protein]
CoA + benzoyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
biotin-CoA + DSLEFIASKLA
D-(biotinyl-4'-phosphopantetheinyl)SLEFIASKLA + ?
show the reaction diagram
biotin-CoA + GDSLDMLEWSLM
GD-(biotinyl-4'-phosphopantetheinyl)SLDMLEWSLM + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRCLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRCLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRLLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRLLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRSLN
GD-(biotinyl-4'-[N-{2-[2-(2-aminoethoxy)ethoxy]ethyl}-3-(2,5-dioxopyrrolidin-1-yl)propanamide]phosphopantetheinyl)SLSWLLRSLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLVRCLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRCLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLVRLLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRLLN + ?
show the reaction diagram
butyryl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde dehydrogenase]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde dehydrogenase]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde reductase Lys2]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde reductase Lys2]
show the reaction diagram
-
-
-
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r
CoA + apo-[EntB-ArCP-H6 E. coli]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
CoA + apo-[fredericamycin H acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[fredericamycin acyl-carrier protein]
show the reaction diagram
-
-
-
?
CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
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r
CoA + apo-[PCPH6SrfB1.18]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
CoA + apo-[PCPH6SrfB2.18]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
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r
CoA + apo-[peptidyl carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
show the reaction diagram
CoA + apo-[peptidyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
show the reaction diagram
-
-
-
-
?
CoA + apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. frenolicin-acyl-carrier protein]
show the reaction diagram
-
-
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r
CoA + apo-[Streptomyces sp. granaticin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. granaticin-acyl-carrier protein]
show the reaction diagram
-
-
-
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r
CoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
show the reaction diagram
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-
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r
CoA + apo-[tetracenomycin M acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[tetracenomycin M acyl-carrier protein]
show the reaction diagram
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein AcpA]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein AcpA]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
?
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
adenosine 3',5'-bisphosphate + holo-[BpsA protein]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[FDH protein]
adenosine 3',5'-bisphosphate + holo-[FDH protein]
show the reaction diagram
-
the enzyme modifies the apo-FDH protein at serine 354 and activates its catalysis
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-
?
crotonyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
decanoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
desulfo-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
desulfoCoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
? + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
homocysteamine-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
malonyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
myristoleoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
myristoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
palmitoleoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
palmitoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
phenylacetyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CoA + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde dehydrogenase]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde dehydrogenase]
show the reaction diagram
-
phosphopantetheinylation of the enzyme involved in lysine catabolism
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-
r
CoA + apo-[alpha-aminoadipate semialdehyde reductase Lys2]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde reductase Lys2]
show the reaction diagram
-
-
-
-
r
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4'-phosphopantetheine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxy-adenosine-3',5'-bisphosphate
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2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-5-fluoro-benzoic acid
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IC50: 0.0021 mM
2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.015 mM
3',5'-ADP
5-bromo-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0015 mM
5-bromo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0034 mM
5-bromo-4-chloro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.002 mM
5-carboxyamino-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.00013 mM
5-chloro-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0011 mM
5-fluoro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0019 mM
5-iodo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0013 mM
5-methyl-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.00027 mM
5-methyl-2-[[5-oxo-2-(3-trifluoromethoxy-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0044 mM
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5-methyl-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
-
IC50: 0.00083 mM
-
6-nitroso-1,2-benzopyrone
6-nitroso-benzopyrone
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adenosine-3',5'-bisphosphate
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apo-acyl-ca