Information on EC 2.7.8.7 - holo-[acyl-carrier-protein] synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.8.7
-
RECOMMENDED NAME
GeneOntology No.
holo-[acyl-carrier-protein] synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acyl carrier protein activation
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acyl carrier protein metabolism
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enterobactin biosynthesis
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lipid metabolism
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Pantothenate and CoA biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
CoA-[4'-phosphopantetheine]:apo-[acyl-carrier protein] 4'-pantetheinephosphotransferase
Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain [3]. The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty acid synthase and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes [6]. Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14, [acyl-carrier-protein] phosphodiesterase.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-30-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
animal
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain FGSC4
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain MP-1
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
no activity in Escherichia coli
strain MP4, denoted acpS1 gene
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Manually annotated by BRENDA team
no activity in Escherichia coli MP4
strain MP4, denoted acpS1 gene
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Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
yeast, strain lys5
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
protozoa
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Automatic Mining of ENzyme DAta
castor-oil-seed, L. cv. Baker 296
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Manually annotated by BRENDA team
strain ATCC24843
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Manually annotated by BRENDA team
Melody
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Manually annotated by BRENDA team
strain DW4/3-1, gene mtaA
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
Streptomyces pneumoniae
strain ATCC15003, nucleotide sequence of the svp locus
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
acetonyldethio-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + acyl-carrier protein
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + apo-[acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
acetyl-CoA + polyketide synthase 1
CoA + acetyl-polyketide synthase 1
show the reaction diagram
acetyl-CoA + polyketide synthase 16
CoA + acetyl-polyketide synthase 16
show the reaction diagram
acetyl-CoA + polyketide synthase 2
CoA + acetyl-polyketide synthase 2
show the reaction diagram
apo-[acyl-carrier protein] + acetyl-CoA
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
pH 7, 37C
reaction stop by 10% trichloroacetic acid, limited release of 3,5-ADP by interactions with guanidinium moieties of R74 and R86
-
?
benzoyl-CoA + apo-[acyl-carrier protein]
CoA + benzoyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
biotin-CoA + DSLEFIASKLA
D-(biotinyl-4'-phosphopantetheinyl)SLEFIASKLA + ?
show the reaction diagram
biotin-CoA + GDSLDMLEWSLM
GD-(biotinyl-4'-phosphopantetheinyl)SLDMLEWSLM + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRCLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRCLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRLLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRLLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLLRSLN
GD-(biotinyl-4'-[N-{2-[2-(2-aminoethoxy)ethoxy]ethyl}-3-(2,5-dioxopyrrolidin-1-yl)propanamide]phosphopantetheinyl)SLSWLLRSLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLVRCLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRCLN + ?
show the reaction diagram
biotin-CoA + GDSLSWLVRLLN
GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRLLN + ?
show the reaction diagram
butyryl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde dehydrogenase]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde dehydrogenase]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde reductase Lys2]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde reductase Lys2]
show the reaction diagram
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-
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r
CoA + apo-[EntB-ArCP-H6 E. coli]
? + holo-[acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[fredericamycin H acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[fredericamycin acyl-carrier protein]
show the reaction diagram
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-
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?
CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
? + holo-[acyl-carrier protein]
show the reaction diagram
-
-
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r
CoA + apo-[PCPH6SrfB1.18]
? + holo-[acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[PCPH6SrfB2.18]
? + holo-[acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[peptidyl carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
show the reaction diagram
CoA + apo-[peptidyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
show the reaction diagram
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-
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?
CoA + apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. frenolicin-acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[Streptomyces sp. granaticin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. granaticin-acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
show the reaction diagram
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-
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r
CoA + apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
show the reaction diagram
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r
CoA + apo-[tetracenomycin M acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[tetracenomycin M acyl-carrier protein]
show the reaction diagram
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-
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?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein AcpA]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein AcpA]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
adenosine 3',5'-bisphosphate + holo-[BpsA protein]
show the reaction diagram
CoA-[4'-phosphopantetheine] + apo-[FDH protein]
adenosine 3',5'-bisphosphate + holo-[FDH protein]
show the reaction diagram
-
the enzyme modifies the apo-FDH protein at serine 354 and activates its catalysis
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?
crotonyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
decanoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
desulfo-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
desulfoCoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
? + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
show the reaction diagram
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-
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r
homocysteamine-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
malonyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
myristoleoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
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-
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?
myristoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
palmitoleoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
palmitoyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
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-
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?
phenylacetyl-CoA + apo-[acyl-carrier protein]
? + holo-[acyl-carrier protein]
show the reaction diagram
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CoA + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
CoA + apo-[alpha-aminoadipate semialdehyde dehydrogenase]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde dehydrogenase]
show the reaction diagram
-
phosphopantetheinylation of the enzyme involved in lysine catabolism
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r
CoA + apo-[alpha-aminoadipate semialdehyde reductase Lys2]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde reductase Lys2]
show the reaction diagram
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-
-
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r
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4'-phosphopantetheine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-5-fluoro-benzoic acid
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IC50: 0.0021 mM
2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.015 mM
3',5'-ADP
5-bromo-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0015 mM
5-bromo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0034 mM
5-bromo-4-chloro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.002 mM
5-carboxyamino-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.00013 mM
5-chloro-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0011 mM
5-fluoro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0019 mM
5-iodo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0013 mM
5-methyl-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.00027 mM
5-methyl-2-[[5-oxo-2-(3-trifluoromethoxy-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.0044 mM
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5-methyl-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
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IC50: 0.00083 mM
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6-nitroso-1,2-benzopyrone
apo-acyl-carrier protein
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
apo-acyl-carrier protein
Streptomyces pneumoniae
significantly stimulating above 0.01 mM
CoA
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stimulates the holoACP synthase reaction within the intact organelle
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0088 - 0.051
acetoacetyl-CoA
0.0077 - 0.399
acetyl-CoA
0.017 - 0.068
apo-ACP
0.0002 - 0.072
apo-acyl-carrier protein
0.002
apo-peptide(1-->74)
-
0.00445 - 0.026
apo-peptidyl carrier protein
0.0008 - 0.0018
apo-[acyl-carrier protein]
0.0033 - 0.0086
apo-[BpsA protein]
-
0.016
apo-[EntB-ArCP-H6 Escherichia coli]
-
pH 6.0, 37C
-
0.0058
apo-[fredericamycin H acyl-carrier protein]
pH 8.0, 37C
0.005
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
-
pH 6.0, 37C
-
0.0018
apo-[PCPH6SrfB2.18]
-
pH 6.0, 37C
-
0.0031 - 0.0039
apo-[peptidyl carrier protein]
0.012
apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
-
pH 7.0, 37C, Escherichia coli acyl carrier protein
0.005
apo-[Streptomyces sp. granaticin-acyl-carrier protein]
-
pH 7.0, 37C, Escherichia coli acyl carrier protein
0.039
apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
-
pH 7.0, 37C, Escherichia coli acyl carrier protein
0.022
apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
-
pH 7.0, 37C, Escherichia coli acyl carrier protein
0.1
apo-[tetracenomycin M acyl-carrier protein]
pH 8.0, 37C
0.007 - 0.035
Butyryl-CoA
0.0011 - 0.15
CoA
0.00062 - 0.011
CoA-[4'-phosphopantetheine]
0.0052 - 0.018
crotonyl-CoA
0.0014 - 0.0015
decanoyl-CoA
0.123 - 0.242
DSLEFIASKLA
0.117 - 0.534
GDSLDMLEWSLM
0.108 - 0.12
GDSLSWLLRCLN
0.0515 - 0.076
GDSLSWLLRLLN
0.221 - 0.254
GDSLSWLLRSLN
0.0772 - 0.139
GDSLSWLVRCLN
0.0618 - 0.105
GDSLSWLVRLLN
0.0071 - 0.094
malonyl-CoA
0.44 - 104.1
Mg2+
0.0025
myristoleoyl-CoA
-
pH 7.5, 22C
0.001 - 0.0018
myristoyl-CoA
0.0009 - 0.0016
palmitoleoyl-CoA
0.0012
palmitoyl-CoA
-
pH 7.5, 22C
additional information
acetyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.2
acetoacetyl-CoA
0.000098 - 0.973
acetyl-CoA
0.0283 - 2.08
apo-acyl-carrier protein
0.239 - 1.6
apo-peptidyl carrier protein
0.002 - 0.7
apo-[acyl-carrier protein]
0.035 - 0.683
apo-[BpsA protein]
-
1.08
apo-[EntB-ArCP-H6 Escherichia coli]
Bacillus subtilis
-
pH 6.0, 37C
-
0.75 - 0.95
apo-[fredericamycin H acyl-carrier protein]
1.17
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
Bacillus subtilis
-
pH 6.0, 37C
-
1.73
apo-[PCPH6SrfB1.18]
Bacillus subtilis
-
pH 6.0, 37C
-
0.933
apo-[PCPH6SrfB2.18]
Bacillus subtilis
-
pH 6.0, 37C
-
0.183 - 1.43
apo-[peptidyl carrier protein]
0.317
apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
Escherichia coli
-
pH 7.0, 37C, E. coli ACP
0.5
apo-[Streptomyces sp. granaticin-acyl-carrier protein]
Escherichia coli
-
pH 7.0, 37C, E. coli ACP
0.167
apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
Escherichia coli
-
pH 7.0, 37C, E. coli ACP
0.09
apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
Escherichia coli
-
pH 7.0, 37C, E. coli ACP
0.004 - 0.3
Butyryl-CoA
0.002 - 2.8
CoA
0.0027 - 0.075
CoA-[4'-phosphopantetheine]
0.005 - 0.2
crotonyl-CoA
0.004 - 0.5
decanoyl-CoA
0.013 - 0.18
DSLEFIASKLA
0.004 - 0.03
GDSLDMLEWSLM
0.001 - 0.17
GDSLSWLLRCLN
0.0005 - 0.17
GDSLSWLLRLLN
0.0013 - 0.14
GDSLSWLLRSLN
0.0007 - 0.07
GDSLSWLVRCLN
0.0005 - 0.05
GDSLSWLVRLLN
0.0004 - 0.1
malonyl-CoA
0.0003 - 1.2
Mg2+
0.5
myristoleoyl-CoA
Streptococcus pneumoniae
-
pH 7.5, 22C
0.003 - 0.5
myristoyl-CoA
0.4
palmitoleoyl-CoA
additional information
additional information
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21.7 - 108
apo-[BpsA protein]
12240
3.3 - 20
CoA-[4'-phosphopantetheine]
6985
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
3',5'-ADP
Streptomyces pneumoniae
pH 7.0, 37C
0.00042 - 0.0052
6-nitroso-1,2-benzopyrone
0.002
apo-acyl-carrier protein
-
pH 7.0, 37C, E. coli ACP
0.054
apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
-
pH 7.0, 37C, E. coli ACP
0.0006
apo-[Streptomyces sp. granaticin-acyl-carrier protein]
-
pH 7.0, 37C, E. coli ACP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021
2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-5-fluoro-benzoic acid
Bacillus subtilis
-
IC50: 0.0021 mM
0.015
2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.015 mM
0.0015
5-bromo-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0015 mM
0.0034
5-bromo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0034 mM
0.002
5-bromo-4-chloro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.002 mM
0.00013
5-carboxyamino-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.00013 mM
0.0011
5-chloro-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0011 mM
0.0019
5-fluoro-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0019 mM
0.0013
5-iodo-2-[[2-(4-chloro-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0013 mM
0.00027
5-methyl-2-[[2-(3-fluoro-4-trifluoromethyl-phenyl)-5-oxo-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.00027 mM
0.0044
5-methyl-2-[[5-oxo-2-(3-trifluoromethoxy-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.0044 mM
-
0.00083
5-methyl-2-[[5-oxo-2-(4-trifluoromethyl-phenyl)-oxazolidin-(4E)-ylidenemethyl]-amino]-benzoic acid
Bacillus subtilis
-
IC50: 0.00083 mM
-
0.0021 - 0.0108
6-nitroso-1,2-benzopyrone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
-
wild-type
additional information
-
specific activity 88.0 units/mg protein
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8.5
-
less than 20% of maximum activity at both pH 5.0 and 7.0, 50% of activity maximum at pH 5.5 and pH 6.8
5 - 7
-
pH 5: less than 27% of maximal activity, pH 7: less than 62% of maximal activity, activity with apo-[acyl-carrier protein]
6 - 11
-
about 20% of activity maximum at pH 6.5, about 30% of activity maximum at pH 11.0
7 - 8.8
-
half maximal activity at pH 7.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 50
Streptomyces pneumoniae
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.25
-
calculated from molar extinction coefficient by the method of Gill and von Hippel
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Mycobacterium ulcerans (strain Agy99)
Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)