Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.8.11 - CDP-diacylglycerol-inositol 3-phosphatidyltransferase and Organism(s) Rattus norvegicus

for references in articles please use BRENDA:EC2.7.8.11
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
phosphatidylinositol synthase, pi synthase, ptdins synthase, cdipt, zmpis, cdp-diglyceride:inositol transferase, cdp-dg:inositol transferase, pkpis, cdp-diacylglycerol-inositol 3-phosphatidyltransferase, bnptdins s1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CDP-DG:inositol transferase
-
-
-
-
CDP-diacylglycerol-inositol phosphatidyltransferase
-
-
-
-
CDP-diacylglycerol:myo-inositol-3-phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol transferase
-
-
-
-
CDP-diglyceride:inositol transferase
-
-
-
-
CDPdiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:myo-inositol 3-phosphatidyltransferase
-
-
-
-
cytidine diphosphodiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol transferase
-
-
-
-
phosphatidylinositol synthase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-01-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-O-(N-heptyl-carbamoyl)methyl-alpha-D-glucopyranoside
-
Hecameg, above 5 mM
bovine serum albumin
-
slight inhibition
-
CDP-diacylglycerol
-
above 0.4 mM
cetyltrimethylammonium bromide
-
strong inhibition
Inosose-2
-
competitive to inositol
Inostamycin
-
-
iodoacetamide
-
-
iodoacetate
-
-
myo-inositol
-
substrate inhibition above 1 mM
N-ethylmaleimide
-
-
p-chloromercuribenzene sulfonate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0095 - 0.21
CDP-diacylglycerol
0.17
CDP-diglyceride
-
pH 8.6, 37°C
0.06 - 2.5
myo-inositol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDIPT_RAT
213
3
23613
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000 - 200000
-
gel filtration
21000
-
? * 21000, SDS-PAGE
23610
300000
-
partially purified enzyme, ultrafiltration
60000
-
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
? * 21000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation caused by repeated freezing and thawing or by contact with detergent is prevented by addition of dithiothreitol and CDPdiacyl-sn-glycerol
-
loses activity when the lipids are removed
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, substrate and dithioerythritol protects the enzyme activity for several days
-
-80°C, can be stored for a few days without appreciable loss of activity, inactivated gradually during storage for longer periods
-
-80°C, partially purified enzyme can be stored for at least 3 months without loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned by functional complementation of a Saccharomyces cerevisiae mutant
-
rat PIS expressed in mouse NIH3T3 fibroblasts by transfection with a FLAG-tagged PIS cDNA using the vector pcDNA3
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rao, R.H.; Strickland, K.P.
On the solubility, stability and partial purification of CDPdiacyl-sn-glycerol: inositol transferase from rat brain
Biochim. Biophys. Acta
348
306-314
1974
Rattus norvegicus
Manually annotated by BRENDA team
Takenawa, T.; Egawa, K.
CDP-diglyceride:inositol transferase from rat liver. Purification and properties
J. Biol. Chem.
252
5419-5423
1977
Rattus norvegicus
Manually annotated by BRENDA team
Cubitt, A.B.; Gershengorn, M.C.
Characterization of a salt-extractable phosphatidylinositol synthase from rat pituitary-tumour membranes
Biochem. J.
257
639-644
1989
Rattus norvegicus
Manually annotated by BRENDA team
Monaco, M.E.; Feldman, M.; Kleinberg, D.L.
Identification of rat liver phosphatidylinositol synthase as a 21 kDa protein
Biochem. J.
304
301-305
1994
Rattus norvegicus
-
Manually annotated by BRENDA team
Antonsson, B.
Phosphatidylinositol synthase from mammalian tissues
Biochim. Biophys. Acta
1348
179-186
1997
Bos taurus, Saccharomyces cerevisiae, Cavia porcellus, Gallus gallus, Oryctolagus cuniculus, Homo sapiens, Meleagris gallopavo, Rattus norvegicus
Manually annotated by BRENDA team
Nikawa, J.; Yamashita, S.
Phosphatidylinositol synthase from yeast
Biochim. Biophys. Acta
1348
173-178
1997
Saccharomyces cerevisiae, no activity in Escherichia coli, Rattus norvegicus
Manually annotated by BRENDA team
Deguchi, A.; Segawa, K.; Hosaka, K.; Weinstein, I.B.; Umezawa, K.
Overexpression of phosphatidylinositol synthase enhances growth and G1 progression in NIH3T3 cells
Jpn. J. Cancer Res.
93
157-166
2002
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team