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Information on EC 2.7.8.11 - CDP-diacylglycerol-inositol 3-phosphatidyltransferase and Organism(s) Arabidopsis thaliana
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2.7.8.11 CDP-diacylglycerol-inositol 3-phosphatidyltransferaseSpecify your search results
Word Map
- 2.7.8.11
- phosphatidylserine
-
3hinositol
-
inositol-containing
-
phospholipid-synthesizing
-
cdp-dag
- inositol-1-phosphate
-
uasino
-
o-phosphatidyltransferase
-
3hptdins
- medicine
- agriculture
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
phosphatidylinositol synthase, pi synthase, ptdins synthase, cdipt, zmpis, cdp-diglyceride:inositol transferase, cdp-dg:inositol transferase, pkpis, cdp-diacylglycerol-inositol 3-phosphatidyltransferase, bnptdins s1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CDP-DG:inositol transferase
-
-
-
-
CDP-diacylglycerol-inositol phosphatidyltransferase
-
-
-
-
CDP-diacylglycerol:myo-inositol-3-phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol transferase
-
-
-
-
CDP-diglyceride:inositol transferase
-
-
-
-
CDPdiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:myo-inositol 3-phosphatidyltransferase
-
-
-
-
cytidine diphosphodiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol transferase
-
-
-
-
phosphatidylinositol synthase
-
-
-
-
PIS1
PIS2
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
substituted phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9027-01-4
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CDP-diheptadecanoylglycerol + D-myo-inositol
CMP + 1,2-diheptadecanoyl-phosphatidyl-1D-myo-inositol
-
-
-
?
CDP-dilinoleoylglycerol + D-myo-inositol
CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
CDP-dipalmitoylglycerol + D-myo-inositol
CMP + 1,2-dipalmitoyl-phosphatidyl-1D-myo-inositol
-
-
-
?
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
key step in lipid biosynthesis
-
-
?
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
D-myo-inositol substrates: phosphatidylethanolamine, phosphatidic acid, overexpressed enzyme in bacterial membrane pellets, 30 min, 22°C, pH 8, 1% (v/v) Triton X-100, 20 mM MgCl2
fatty acid analysis by thin-layer chromatography
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
-
r
CDP-dilinoleoylglycerol + D-myo-inositol
CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
-
-
-
?
CDP-dilinoleoylglycerol + D-myo-inositol
CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dioleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol
-
-
?
CDP-dioleoylglycerol + D-myo-inositol
CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
-
-
-
?
CDP-dioleoylglycerol + D-myo-inositol
CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dilinoleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol
-
-
?
additional information
?
-
-
enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol
-
-
?
additional information
?
-
highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid
-
-
?
additional information
?
-
highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid
-
-
?
additional information
?
-
-
highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid
-
-
?
additional information
?
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants
-
-
?
additional information
?
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants
-
-
?
additional information
?
-
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants
-
-
?
additional information
?
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants
-
-
?
additional information
?
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants
-
-
?
additional information
?
-
-
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants
-
-
?
additional information
?
-
moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid
-
-
?
additional information
?
-
moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid
-
-
?
additional information
?
-
-
moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid
-
-
?
additional information
?
-
preference of C18-fatty acid CDP-diacylglycerol substrates
-
-
?
additional information
?
-
preference of C18-fatty acid CDP-diacylglycerol substrates
-
-
?
additional information
?
-
-
preference of C18-fatty acid CDP-diacylglycerol substrates
-
-
?
additional information
?
-
preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates
-
-
?
additional information
?
-
preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates
-
-
?
additional information
?
-
-
preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
key step in lipid biosynthesis
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene AtPIS2, putative phosphatidylinositol synthase that appears to be encoded by a genomic fragment present in BAC clone F20m13
UniProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, limited overlap with marker (rat sialyl-S-transferase)
transient gene expression in onion epidermal cells, C-terminal CFP-tag, confocal microscopy, centre of punctuate structures
transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, periphery of punctuate structures
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PIS1_ARATH
227
4
25861
Swiss-Prot
other Location (Reliability: 5)
PIS2_ARATH
225
4
25580
Swiss-Prot
Secretory Pathway (Reliability: 2)
A0A178UUZ3_ARATH
225
4
25580
TrEMBL
Secretory Pathway (Reliability: 2)
A0A5S9WSN1_ARATH
227
4
25861
TrEMBL
other Location (Reliability: 5)
F4JTR2_ARATH
217
4
24695
TrEMBL
Secretory Pathway (Reliability: 2)
A0A178WC27_ARATH
227
4
25861
TrEMBL
other Location (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C148W
polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
as bacterial membrane pellets, no further specific purification possible
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtPIS1 cDNA cloned in Escherichia coli XL-1 Blue, functional complementation of Saccharomyces cerevisiae wild-type strain D501-1
AtPIS1 cloned and expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol
-
from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP for introduction of C-terminal EYFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections
from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP or -CFP for introduction of C-terminal EYFP- or CFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xue, H.W.; Hosaka, K.; Plesch, G.; Mueller-Roeber, B.
Cloning of Arabidopsis thaliana phosphatidylinositol synthase and functional expression in the yeast pis mutant
Plant Mol. Biol.
42
757-764
2000
Arabidopsis thaliana, Arabidopsis thaliana (Q8GUK6), no activity in Saccharomyces cerevisiae
Justin, A.M.; Kader, J.C.; Collin, S.
Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis
Eur. J. Biochem.
269
2347-2352
2002
Arabidopsis thaliana, no activity in Escherichia coli
Justin, A.M.; Kader, J.C.; Collin, S.
Synthetic capacity of Arabidopsis phosphatidylinositol synthase 1 expressed in Escherichia coli
Biochim. Biophys. Acta
1634
52-60
2003
Arabidopsis thaliana
Loefke, C.; Ischebeck, T.; Koenig, S.; Freitag, S.; Heilmann, I.
Alternative metabolic fates of phosphatidylinositol produced by phosphatidylinositol synthase isoforms in Arabidopsis thaliana
Biochem. J.
413
115-124
2008
Arabidopsis thaliana (Q8GUK6), Arabidopsis thaliana (Q8LBA6), Arabidopsis thaliana
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