Information on EC 2.7.7.9 - UTP-glucose-1-phosphate uridylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.7.9
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RECOMMENDED NAME
GeneOntology No.
UTP-glucose-1-phosphate uridylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Biosynthesis of antibiotics
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Galactose metabolism
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Metabolic pathways
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Pentose and glucuronate interconversions
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stachyose degradation
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Starch and sucrose metabolism
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sucrose biosynthesis II
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sucrose degradation II (sucrose synthase)
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type I lipoteichoic acid biosynthesis (S. aureus)
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UDP-alpha-D-glucose biosynthesis I
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degradation of hexoses
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glycogen biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UTP:alpha-D-glucose-1-phosphate uridylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-22-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Acetabularia sp.
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Manually annotated by BRENDA team
Mill. x Annona squamosa L.
UniProt
Manually annotated by BRENDA team
Annona emarginata
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Manually annotated by BRENDA team
var. Landsberg erecta, gene UGP1, a sucrose-induced gene
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Manually annotated by BRENDA team
isolated from sea mud
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Manually annotated by BRENDA team
isolated from sea mud
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Manually annotated by BRENDA team
sugar beet
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
pigeon
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-
Manually annotated by BRENDA team
in addition to UGPase, presence of dual substrate UDP-hexose pyrophosphorylase
SwissProt
Manually annotated by BRENDA team
cv. Calypiso
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Golgi apparatus and cytosolic isozyme
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain 26 695
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
lamb
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
isoforms UGP1, UGP2
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-
Manually annotated by BRENDA team
Saccharomyces fragilis
galactose-adopted
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Manually annotated by BRENDA team
cv. Rox Orange
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
subsp. zooepidemicus. i.e. Streptococcus zooepidemicus
UniProt
Manually annotated by BRENDA team
strain KCCM 11405, recombinant protein. Enzyme is part of the validamycin A biosynthetic pathway
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Manually annotated by BRENDA team
strain KCCM 11405, recombinant protein. Enzyme is part of the validamycin A biosynthetic pathway
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Manually annotated by BRENDA team
strain LY03
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Manually annotated by BRENDA team
strain LY03
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Manually annotated by BRENDA team
wild-type and mutant RN-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Q4UVD0
SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-glucose
show the reaction diagram
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13.7% of the activity with UTP
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r
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
show the reaction diagram
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
show the reaction diagram
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
show the reaction diagram
diphosphate + UDP-galactose
UTP + alpha-D-galactose 1-phosphate
show the reaction diagram
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-
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r
diphosphate + UDP-glucose
diphosphate + UDP-glucose
show the reaction diagram
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r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
show the reaction diagram
diphosphate + UDP-mannose
UTP + D-mannose 1-phosphate
show the reaction diagram
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
show the reaction diagram
farnesyl triphosphate + UDP-glucose
farnesyl-tetraphosphouridine + alpha-D-glucose + diphosphate
show the reaction diagram
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-
-
-
?
geranyl triphosphate + UDP-glucose
geranyl-tetraphosphouridine + alpha-D-glucose + diphosphate
show the reaction diagram
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-
-
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?
isopentenyl triphosphate + UDP-glucose
isopentenyl-tetraphosphouridine + alpha-D-glucose + diphosphate
show the reaction diagram
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-
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?
methylenebisphosphonate + UDP-glucose
uridine 5'-(beta,gamma-methylenetriphosphate) + alpha-D-glucose + diphosphate
show the reaction diagram
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-
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?
monothiodiphosphate + UDP-glucose
UTPbeta,gammaS + alpha-D-glucose 1-phosphate
show the reaction diagram
-
poor substrate, 4.8% of Vmax with diphosphate
UTPbeta,gammaS is chemically unstable and undergoes hydrolysis to UDPbetaS and phosphate, which renders the otherwise reversible reaction irreversible, t1/2 at 25C: 0.5 min
ir
TTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-TDP-glucose
show the reaction diagram
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
show the reaction diagram
UDP-glucose + adenosine 5'-tetraphosphate
D-glucose-1-phosphate + adenosyl-5'-pentaphospho-5'-uridine
show the reaction diagram
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-
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?
UDP-glucose + ATP
D-glucose-1-phosphate + adenosyl-5'-tetraphospho-5'-uridine
show the reaction diagram
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?
UDP-glucose + CTP
D-glucose-1-phosphate + cytidinyl-5'-tetraphospho-5'-uridine
show the reaction diagram
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-
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?
UDP-glucose + GTP
D-glucose-1-phosphate + guanosyl-5'-tetraphospho-5'-uridine
show the reaction diagram
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?
UDP-glucose + guanosine 5'-tetraphosphate
D-glucose-1-phosphate + guanosyl-5'-pentaphospho-5'-uridine
show the reaction diagram
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-
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?
UDP-glucose + tetrapolyphosphate
uridine-5'-pentaphosphate + D-glucose-1-phosphate
show the reaction diagram
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?
UDP-glucose + tripolyphosphate
uridine-5'-tetraphosphate + D-glucose-1-phosphate
show the reaction diagram
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?
UTP + alpha-D-galactose 1-phosphate
diphosphate + alpha-UDP-galactose
show the reaction diagram
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
show the reaction diagram
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-UDP-glucose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
UTP + alpha-D-glucose 1-phosphate
show the reaction diagram
enzyme is highly specific for both UTP and glucose 1-phosphate
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r
UTP + D-glucose-1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
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?
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
UTP + glucose-1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-UDP-glucose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Magnesium
Q6M6R3
crystallization data, two magnesium ions coordinate to UDP-glucose. Magnesium is involved in catalytic mechanism
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-phosphoglycerate
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no inhibition of UDP-glucose synthesis
ADP
in the presence of ATP or ADP, the activity decreases to about 60%
alpha-D-galactose 1-phosphate
alpha-D-glucose 1-phosphate
ATP
in the presence of ATP or ADP, the activity decreases to about 60%
Ca2+
about 40% inhibition
D-fructose 2,6-bisphosphate
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no inhibition of UDP-glucose synthesis
D-fructose 6-phosphate
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D-galactosamine
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D-galactose 6-phosphate
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UDP-glucose pyrophosphorolysis
diphosphate
H2O2
complete loss of activity after 30 min, inactivation is reverted by reduction by cysteine and thioredoxin
iodoacetamide
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wild-type: loss of 56% activity after 30 min at 0.1 mM, mutant C123S is not affected
Mg-diphosphate
MgUTP
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wild-type and mutants C123S, H266R, W218, R389H, R422Q, R445H
MgUTP2-
NAD+
presence of either NAD or NADP results in a decrease of activity to about 80% or 50%, respectively
NADP+
presence of either NAD or NADP results in a decrease of activity to about 80% or 50%, respectively
phosphate
sodium nitroprusside
complete loss of activity after 30 min, inactivation is reverted by reduction by cysteine and thioredoxin
Sucrose
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54% inhibition at 2%
TDP-glucose
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TDP-rhamnose
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UDP-D-mannose
UDP-galactose
UDP-galacturonic acid
UDP-glucose
UDP-glucuronic acid
UDP-mannose
UDP-xylose
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
3-phosphoglycerate
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slight activation
Co2+
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no effect on liver enzyme form I, 2fold activation of enzyme form from sublingual gland, inhibition of enzyme form from submandibular gland
D-erythrose 4-phosphate
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slight activation
D-fructose 6-phosphate
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slight activation
dithiothreitol
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increases wild-type activity by about 25%
DTT
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requirement
Sucrose
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activates; inducible by
Triton X-100
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activation, only membrane-bound Golgi-enzyme
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 10
alpha-D-galactose 1-phosphate
0.01 - 1
alpha-D-glucose 1-phosphate
0.19 - 0.83
D-glucose-1-phosphate
0.027 - 60
diphosphate
0.32
farnesyl triphosphate
-
pH 8.0, 30C
0.21
geranyl triphosphate
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pH 8.0, 30C
0.51
isopentenyl triphosphate
-
pH 8.0, 30C
1.1
methylenebisphosphonate
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pH 8.0, 30C
0.33 - 0.56
Mg-diphosphate
0.22
Mg-UTP
-
UDP-glucose synthesis
0.58 - 1.11
Mg2+
0.56
MgUTP
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recombinant wild-type enzyme, pH 8.0, 25C
4.5
tripolyphosphate
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30C, pH 8.0
0.35 - 2
TTP
0.26 - 0.42
UDP-galactose
0.00167 - 57
UDP-glucose
0.0075 - 0.53
UTP
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45 - 55.03
alpha-D-glucose 1-phosphate
6.35
diphosphate
Nostoc sp.
Q8YS18
pH 7.5, 37C
0.033
farnesyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
0.038
geranyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
0.013
isopentenyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
27
methylenebisphosphonate
Saccharomyces cerevisiae
-
pH 8.0, 30C
6.8 - 10
TTP
0.21 - 19.98
UDP-glucose
0.47 - 93.44
UTP
additional information
additional information
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.81 - 1240
alpha-D-glucose 1-phosphate
107
15.1
diphosphate
Nostoc sp.
Q8YS18
pH 7.5, 37C
17
0.103
farnesyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
8234
0.181
geranyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
8877
0.025
isopentenyl triphosphate
Saccharomyces cerevisiae
-
pH 8.0, 30C
8876
24.55
methylenebisphosphonate
Saccharomyces cerevisiae
-
pH 8.0, 30C
5782
18.3 - 28.3
TTP
523
2.09 - 11980
UDP-glucose
64
1.38 - 7870
UTP
65
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.135
alpha-D-glucose 1-phosphate
-
pH 8.0, 30C
0.213 - 0.95
diphosphate
0.095 - 0.42
MgUTP2-
10.9 - 12.2
phosphate
4.8
UDP-D-galactose
0.21 - 0.93
UDP-D-galacturonic acid
0.005 - 0.13
UDP-D-glucose
0.15 - 0.75
UDP-D-glucuronic acid
9.6
UDP-D-mannose
0.24 - 1.6
UDP-D-xylose
0.015 - 0.12
UDP-glucose
0.104 - 0.17
UTP
additional information
additional information
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