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Information on EC 2.7.7.7 - DNA-directed DNA polymerase and Organism(s) Mus musculus
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2.7.7.7 DNA-directed DNA polymeraseIUBMB Comments
Catalyses DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Requires a primer, which may be DNA or RNA. See also EC 2.7.7.49 RNA-directed DNA polymerase.
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Word Map
- 2.7.7.7
- strand
-
single-stranded
-
fidelity
-
holoenzyme
- mismatch
- bacteriophage
- pcna
- fork
- polymerization
- aphidicolin
- duplex
-
proofreading
-
calf
- endonuclease
- thymus
-
clamp
-
misincorporation
- helicase
-
abasic
-
template-primer
- 5'-triphosphate
- dntps
-
stall
- thymine
-
error-free
- mispairs
-
anneal
-
sliding
-
uv-induced
-
incoming
-
exonucleolytic
- replisome
-
transversions
- deoxyribonucleoside
- xeroderma
- pigmentosum
- ssdna
-
deoxynucleotide
-
hypermutation
-
undamaged
-
okazaki
- dtmp
-
slippage
-
cyclobutane
- transcriptases
- dnab
-
watson-crick
-
high-fidelity
-
myeloblastosis
- aquaticus
- synthesis
- analysis
- drug development
- diagnostics
- biotechnology
- medicine
- molecular biology
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
dna polymerase alpha, dna polymerase beta, dna polymerase iii, pol beta, klenow fragment, dna polymerase delta, taq dna polymerase, pol delta, pol alpha, dna polymerase gamma, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
deoxynucleate polymerase
-
-
-
-
deoxyribonucleate nucleotidyltransferase
-
-
-
-
deoxyribonucleic acid duplicase
-
-
-
-
deoxyribonucleic acid polymerase
-
-
-
-
deoxyribonucleic duplicase
-
-
-
-
deoxyribonucleic polymerase
-
-
-
-
deoxyribonucleic polymerase I
-
-
-
-
DNA duplicase
-
-
-
-
DNA nucleotidyltransferase
-
-
-
-
DNA nucleotidyltransferase (DNA-directed)
-
-
-
-
DNA polmerase beta
-
-
-
-
DNA polymerase
-
-
-
-
DNA polymerase alpha
-
-
-
-
DNA polymerase gamma
-
-
-
-
DNA polymerase I
-
-
-
-
DNA polymerase II
-
-
-
-
DNA polymerase III
-
-
-
-
DNA replicase
-
-
-
-
DNA-dependent DNA polymerase
-
-
-
-
duplicase
-
-
-
-
Klenow fragment
-
-
-
-
nucleotidyltransferase, deoxyribonucleate
-
-
-
-
Pol gamma
-
-
-
-
sequenase
-
-
-
-
Taq DNA polymerase
-
-
-
-
Taq Pol I
-
-
-
-
Tca DNA polymerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)
Catalyses DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Requires a primer, which may be DNA or RNA. See also EC 2.7.7.49 RNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY
9012-90-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
beta-polymerase can copy a synthetic ribohomopolymer such as (A)n*(dT)12 as well as the corresponding deoxyribohomopolymer (dA)n*(dT)12 or activated DNA, alpha-polymerase utilizes the deoxyribohomopolymer (dA)n*dT12-18 eight times better than (A)n*dT12
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
sulfoglycolipid beta-sulfoquinovosyldiacylglycerol
-
inhibits the DNA polymerase activity of pol lambda by binding to the Met1-Arg95 region and inhibiting its nuclear transit
-
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dimethyl sulfoxide
-
stimulates DNA polymerase alpha and delta, inhibits human DNA polymerase epsilon
proliferating cell nuclear antigen
-
PCNA, specific auxiliary factor stimulating DNA polymerase delta
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mouse strains defective in specialized DNA polymerases are used for studying their function
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
DNA polymerase beta localizes to the synaptonemal complex during prophase I of meiosis, the enzyme localizes to synapsed axes during zygonema and pachynema, and it associates with the ends of bivalents during late pachynema and diplonema
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
-
class switch recombination is normal in mice deficient for pols theta and eta
malfunction
-
Pol beta-deficient spermatocytes are defective in meiotic chromosome synapsis and undergo apoptosis during prophase I, Pol beta-deficient seminferous tubules have few germ cells, Pol beta-deficient spermatocytes do not progress through meiosis, Pol beta-deficient mice are fertile
physiological function
-
DNA polymerase beta is critical for mouse meiotic synapsis, Pol beta is required at a very early step in the processing of meiotic double-strand breaks, at or before the removal of SPO11 from double-strand break ends and the generation of the 3' single-stranded tails necessary for subsequent strand exchange
physiological function
-
DNA polymerase eta is a limiting factor for A:T mutations in immunglobulin genes and contributes to antibody affinity maturation in germinal center B cells
physiological function
-
pol theta has no major involvement in somatic hypermutation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DPOLA_MOUSE
1465
0
167340
Swiss-Prot
other Location (Reliability: 1)
POLI_MOUSE
717
0
79653
Swiss-Prot
other Location (Reliability: 5)
POLK_MOUSE
852
0
96003
Swiss-Prot
other Location (Reliability: 1)
DPOG1_MOUSE
1218
0
136758
Swiss-Prot
Mitochondrion (Reliability: 3)
DPOLB_MOUSE
335
0
38288
Swiss-Prot
other Location (Reliability: 2)
DPOLL_MOUSE
573
0
62943
Swiss-Prot
other Location (Reliability: 2)
DPOLM_MOUSE
496
0
55490
Swiss-Prot
Mitochondrion (Reliability: 1)
DPOLN_MOUSE
864
0
96675
Swiss-Prot
other Location (Reliability: 1)
REV3L_MOUSE
3122
0
350711
Swiss-Prot
other Location (Reliability: 2)
DPOLQ_MOUSE
2544
0
280714
Swiss-Prot
Mitochondrion (Reliability: 4)
DPOD1_MOUSE
1105
0
123790
Swiss-Prot
other Location (Reliability: 3)
POLH_MOUSE
694
0
76167
Swiss-Prot
other Location (Reliability: 4)
DPOE1_MOUSE
2283
0
262100
Swiss-Prot
other Location (Reliability: 5)
D6RFB8_MOUSE
981
0
108868
TrEMBL
other Location (Reliability: 3)
Q05BB8_MOUSE
1013
0
113401
TrEMBL
Mitochondrion (Reliability: 3)
Q8CD66_MOUSE
716
0
82152
TrEMBL
other Location (Reliability: 2)
Q5NCI3_MOUSE
511
0
56496
TrEMBL
Mitochondrion (Reliability: 1)
Q75WC0_MOUSE
1217
0
136622
TrEMBL
Mitochondrion (Reliability: 3)
Q7TN90_MOUSE
496
0
55474
TrEMBL
Mitochondrion (Reliability: 1)
E9QAP6_MOUSE
407
0
46088
TrEMBL
other Location (Reliability: 2)
F6T1D2_MOUSE
807
0
90945
TrEMBL
other Location (Reliability: 2)
Q3UWI6_MOUSE
863
0
98337
TrEMBL
other Location (Reliability: 4)
E9QAP5_MOUSE
193
0
22251
TrEMBL
other Location (Reliability: 5)
Q3TRT4_MOUSE
577
0
66096
TrEMBL
Mitochondrion (Reliability: 5)
Q924W4_MOUSE
496
0
55593
TrEMBL
Mitochondrion (Reliability: 1)
Q91VT0_MOUSE
1105
0
123744
TrEMBL
other Location (Reliability: 3)
A0A0J9YU03_MOUSE
261
1
29041
TrEMBL
Mitochondrion (Reliability: 3)
Q80XH7_MOUSE
2283
0
262116
TrEMBL
other Location (Reliability: 5)
A0A0J9YU70_MOUSE
69
0
7685
TrEMBL
other Location (Reliability: 1)
Q3TBY2_MOUSE
410
0
46424
TrEMBL
other Location (Reliability: 2)
Q80XB7_MOUSE
2544
0
280714
TrEMBL
Mitochondrion (Reliability: 4)
Q3UZX3_MOUSE
1217
0
136593
TrEMBL
Mitochondrion (Reliability: 3)
Q3TMK8_MOUSE
193
0
22309
TrEMBL
other Location (Reliability: 5)
Q99LQ3_MOUSE
320
0
36030
TrEMBL
other Location (Reliability: 4)
Q3UG70_MOUSE
1217
0
136602
TrEMBL
Mitochondrion (Reliability: 3)
A0A0J9YV64_MOUSE
94
0
10822
TrEMBL
other Location (Reliability: 2)
Q3UPI9_MOUSE
1198
0
137186
TrEMBL
other Location (Reliability: 4)
F7BP45_MOUSE
243
0
27367
TrEMBL
other Location (Reliability: 1)
Q8C2N0_MOUSE
1105
0
123776
TrEMBL
other Location (Reliability: 3)
D6RIP1_MOUSE
498
0
56301
TrEMBL
Mitochondrion (Reliability: 3)
Q7TQC0_MOUSE
2587
0
285866
TrEMBL
other Location (Reliability: 3)
A0A0J9YTZ6_MOUSE
155
0
18028
TrEMBL
other Location (Reliability: 4)
Q3UR94_MOUSE
1273
0
146657
TrEMBL
other Location (Reliability: 5)
F6YLN2_MOUSE
236
0
26212
TrEMBL
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
microbatch method, sitting-vapor diffusion method. 2.4 A crystal structure of the polymerase domain of murine Pol l bound to gapped DNA with a correct dNTP at the active site
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
H329A
-
mutation has little effect on template-dependent synthesis by Pol l from a paired primer terminus, but it reduces both template-independent and template-dependent synthesis during nonhomologous DNA end joining of intermediates whose 3' ends lack complementary template strand nucleotides
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+ chelate affinity chromatography and glutathione sepharose affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Weissbach, A.
Cellular and viral-induced eukaryotic polymerases
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
14
67-86
1981
Bos taurus, Gallus gallus, Herpes simplex virus, Homo sapiens, Mammalia, Mus musculus, Rattus norvegicus, Vaccinia virus
-
Lehman, I.R.; Karguni, L.S.
DNA polymerase alpha
J. Biol. Chem.
264
4265-4268
1989
Chlorocebus aethiops, Bos taurus, Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Mammalia, Mus musculus
Bambara, R.A.; Jessee, C.B.
Properties of DNA polymerases delta and epsilon, and their roles in eukaryotic DNA replication
Biochim. Biophys. Acta
1088
11-24
1991
Bos taurus, Saccharomyces cerevisiae, Oryctolagus cuniculus, Homo sapiens, Mammalia, Mus musculus
Fischhaber, P.L.; McDaniel, L.D.; Friedberg, E.C.
DNA polymerases for translesion DNA synthesis: enzyme purification and mouse models for studying their function
Methods Enzymol.
408
355-378
2006
Mus musculus
Moon, A.F.; Garcia-Diaz, M.; Bebenek, K.; Davis, B.J.; Zhong, X.; Ramsden, D.A.; Kunkel, T.A.; Pedersen, L.C.
Structural insight into the substrate specificity of DNA polymerase mu
Nat. Struct. Mol. Biol.
14
45-53
2007
Mus musculus
Makarova, A.V.; Tarantul, V.Z.; Gening, L.V.
Evolution of DNA polymerase iota structure and function in eukaryotes
Biochemistry
73
346-352
2008
Canis lupus familiaris, Gallus gallus, Coturnix coturnix, Oryctolagus cuniculus, Misgurnus fossilis, Mus musculus, Rana temporaria, Rattus norvegicus, Triturus cristatus, Testudines agrionemys, Philothamnus punctatus
Martomo, S.A.; Saribasak, H.; Yokoi, M.; Hanaoka, F.; Gearhart, P.J.
Reevaluation of the role of DNA polymerase theta in somatic hypermutation of immunoglobulin genes
DNA Repair
7
1603-1608
2008
Mus musculus
Kidane, D.; Jonason, A.S.; Gorton, T.S.; Mihaylov, I.; Pan, J.; Keeney, S.; de Rooij, D.G.; Ashley, T.; Keh, A.; Liu, Y.; Banerjee, U.; Zelterman, D.; Sweasy, J.B.
DNA polymerase beta is critical for mouse meiotic synapsis
EMBO J.
29
410-423
2010
Mus musculus
Masuda, K.; Ouchida, R.; Yokoi, M.; Hanaoka, F.; Azuma, T.; Wang, J.Y.
DNA polymerase eta is a limiting factor for A:T mutations in Ig genes and contributes to antibody affinity maturation
Eur. J. Immunol.
38
2796-2805
2008
Mus musculus
Takakusagi, K.; Takakusagi, Y.; Ohta, K.; Aoki, S.; Sugawara, F.; Sakaguchi, K.
A sulfoglycolipid beta-sulfoquinovosyldiacylglycerol (betaSQDG) binds to Met1-Arg95 region of murine DNA polymerase lambda (Mmpol lambda) and inhibits its nuclear transit
Protein Eng. Des. Sel.
23
51-60
2010
Mus musculus
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