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Information on EC 2.7.7.52 - RNA uridylyltransferase and Organism(s) Homo sapiens

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     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.52 RNA uridylyltransferase
IUBMB Comments
The enzyme requires an oligoribonucleotide or polyribonucleotide with a free terminal 3'-OH as a primer.
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Homo sapiens
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Word Map
  • 2.7.7.52
  • social
  • women
  • clinician
  • services
  • children
  • plan
  • physician
  • fabric
  • healthcare
  • perceived
  • professionals
  • decision
  • percept
  • demographic
  • film
  • user
  • theme
  • policy
  • psychological
  • skill
  • evidence-based
  • team
  • practitioner
  • surgeon
  • nanostructures
  • decision-making
  • emotional
  • psychosocial
  • thematic
  • tunable
  • semi-structured
  • nurses
  • rehabilitation
  • caregiver
  • satisfaction
  • dope
  • doping
  • stakeholder
  • semiconductor
  • self-management
  • optoelectronic
  • self-efficacy
  • sociodemographic
  • therapist
  • photoluminescence
  • violence
  • psycinfo
  • interpersonal
  • curriculum
  • self-care
  • medicine
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
tailor, tutase, zcchc11, terminal uridylyl transferase, heso1, rna editing tutase, terminal uridylyltransferase, meat1, tutase 1, u6-tutase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GLD2
-
-
terminal uridylyl transferase
-
-
terminal uridylyltransferase
TUT
-
-
-
-
TUT2
-
-
TUTase
U6 snRNA-specific terminal uridylyltransferase
-
U6-TUTase
-
U6 snRNA-specific terminal uridylyltransferase
uridylyltransferase, terminal
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UTP:RNA uridylyltransferase
The enzyme requires an oligoribonucleotide or polyribonucleotide with a free terminal 3'-OH as a primer.
CAS REGISTRY NUMBER
COMMENTARY hide
78519-53-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (mRNA)n
diphosphate + (mRNA)n+1
show the reaction diagram
-
i.e. total human mRNA
-
-
?
ATP + 5'(ppp)-UGAGGUAGUAGGUUGUAUAGUU-3'
diphosphate + 5'(ppp)-UGAGGUAGUAGGUUGUAUAGUUA-3'
show the reaction diagram
-
i.e. triphosphorylated human let-7a-5p-3p
-
-
?
ATP + 5'-UGAGGUAGUAGGUUGUAUAGUU-3'
diphosphate + 5'-UGAGGUAGUAGGUUGUAUAGUUA-3'
show the reaction diagram
-
i.e. unphosphorylated human let-7a-0P. Gld2 displays an 83fold preference of ATP over UTP
-
-
?
ATP + U6 small nuclear RNA
?
show the reaction diagram
isoform TUT1 incorporates UMP more efficiently than AMP into U6 small nuclear RNA transcript ending with four uridines
-
-
?
CTP + 5'(pp)-UGAGGUAGUAGGUUGUAUAGUU-3'
diphosphate + 5'(pp)-UGAGGUAGUAGGUUGUAUAGUUC-3'
show the reaction diagram
-
i.e. diphosphorylated human let-7a-5p-2p
-
-
?
GTP + miR-122
diphosphate + ?
show the reaction diagram
-
-
-
-
?
UTP + 5'(p)-UGAGGUAGUAGGUUGUAUAGUU-3'
diphosphate + 5'(p)-UGAGGUAGUAGGUUGUAUAGUUU-3'
show the reaction diagram
-
i.e. monophosphorylated human let-7a-5p. Gld2 displays an 83fold preference of ATP over UTP
-
-
?
UTP + miR-324n
diphosphate + miR-324n+1
show the reaction diagram
-
-
-
?
UTP + miRNAn
diphosphate + miRNAn+1
show the reaction diagram
-
-
-
?
UTP + mRNAn
diphosphate + mRNAn+1
show the reaction diagram
-
-
-
-
?
UTP + precursor let-7 RNA
?
show the reaction diagram
-
-
-
?
UTP + precursor let-7 RNAn
diphosphate + precursor let-7 RNAn+1
show the reaction diagram
the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7
-
-
?
UTP + precursor let-7a-1 RNAn
diphosphate + precursor let-7a-1 RNAn+1
show the reaction diagram
-
-
-
?
UTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
UTP + U6 small nuclear RNA
?
show the reaction diagram
isoform TUT1 incorporates UMP more efficiently than AMP into U6 small nuclear RNA transcript ending with four uridines
-
-
?
UTP + U6 snRNAn
diphosphate + U6 snRNAn+1
show the reaction diagram
the enzyme builds or repairs the 3'-oligo-uridylylated tail of U6 snRNA
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UTP + miR-324n
diphosphate + miR-324n+1
show the reaction diagram
-
-
-
?
UTP + miRNAn
diphosphate + miRNAn+1
show the reaction diagram
-
-
-
?
UTP + mRNAn
diphosphate + mRNAn+1
show the reaction diagram
-
-
-
-
?
UTP + precursor let-7 RNAn
diphosphate + precursor let-7 RNAn+1
show the reaction diagram
the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7
-
-
?
UTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
UTP + U6 snRNAn
diphosphate + U6 snRNAn+1
show the reaction diagram
the enzyme builds or repairs the 3'-oligo-uridylylated tail of U6 snRNA
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
contains zinc
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ionic strength
-
stimulates, e.g. 600 mM KCl
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000228
ATP
-
pH 8.0, 37°C
0.055
CTP
-
pH 8.0, 37°C
0.23
GTP
-
pH 8.0, 37°C
0.000055
U6 snRNAn
wild type enzyme, at pH 8.5 and 37°C
-
0.169
UTP
-
pH 8.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000293
ATP
-
pH 8.0, 37°C
0.000914
CTP
-
pH 8.0, 37°C
0.000403
GTP
-
pH 8.0, 37°C
0.061
U6 snRNAn
wild type enzyme, at pH 8.5 and 37°C
-
0.000248
UTP
-
pH 8.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.128
ATP
-
pH 8.0, 37°C
0.0166
CTP
-
pH 8.0, 37°C
0.0018
GTP
-
pH 8.0, 37°C
0.0015
UTP
-
pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.9
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
STPAP_HUMAN
874
0
93847
Swiss-Prot
other Location (Reliability: 2)
TUT4_HUMAN
1644
0
185166
Swiss-Prot
other Location (Reliability: 1)
TUT7_HUMAN
1495
0
171229
Swiss-Prot
other Location (Reliability: 1)
F5H0R1_HUMAN
912
0
98435
TrEMBL
other Location (Reliability: 3)
A0A0A8K9B1_HUMAN
874
0
93847
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
-
x * 115000, U6-TUTase, SDS-PAGE
68000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 115000, U6-TUTase, SDS-PAGE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apoenzyme and enzyme in complex with UTP or ATP, hanging drop vapor diffusion method, using 13-15% (w/v) PEG3350 as the precipitant
Lin28-interacting module of TUT4, sitting drop vapor diffusion method, using 100 mM HEPES-NaOH, pH 7.0, 20% (w/v) PEG3350, 3% (v/v) 2-methyl-2,4-pentanediol, 200mM ammonium citrate, and 3% (w/v) 1,5 diaminopentane dihydrochloride
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C306A/C309A
the mutant does not oligo-uridylylate pre-let-7a-1 RNA in the presence of Lin28A
H405A/K452A
the mutant shows reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme
K321A/K324A
the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme
K326A/R327A
the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme
K329A/K330A
the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme
R669A/R670A
the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme
R779A/R783A
the mutations reduce the RNA-binding and uridylylation activities of the enzyme
R871A/K874A
the mutations reduce the RNA-binding activity of the enzyme
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 25% glycerol, stable
-
4°C, does not tolerate more than several h at
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2400fold to near homogeneity
-
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration
partial purification of U6 snRNA specific enzyme form via chromatography on a U6 snRNA affinity resin, separation from unspecific enzyme form
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in HEK-293 cell
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Andrews, N.C.; Baltimore, D.
Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction
Proc. Natl. Acad. Sci. USA
83
221-225
1986
Homo sapiens
Manually annotated by BRENDA team
Trippe, R.; Richly, H.; Benecke, B.J.
Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase
Eur. J. Biochem.
270
971-980
2003
Homo sapiens
Manually annotated by BRENDA team
Heo, I.; Ha, M.; Lim, J.; Yoon, M.; Park, J.; Kwon, S.; Chang, H.; Kim, V.
Mono-uridylation of pre-microRNA as a key step in the biogenesis of group II let-7 microRNAs
Cell
151
521-532
2012
Homo sapiens (Q5TAX3), Homo sapiens (Q5VYS8)
Manually annotated by BRENDA team
Thornton, J.E.; Chang, H.M.; Piskounova, E.; Gregory, R.I.
Lin28-mediated control of let-7 microRNA expression by alternative TUTases Zcchc11 (TUT4) and Zcchc6 (TUT7)
RNA
18
1875-1885
2012
Homo sapiens (Q5TAX3), Homo sapiens (Q5VYS8)
Manually annotated by BRENDA team
Chung, C.Z.; Jo, D.H.; Heinemann, I.U.
Nucleotide specificity of the human terminal nucleotidyltransferase Gld2 (TUT2)
RNA
22
1239-1249
2016
Homo sapiens
Manually annotated by BRENDA team
Yashiro, Y.; Tomita, K.
Function and regulation of human terminal uridylyltransferases
Front. Genet.
9
538
2018
Homo sapiens (F5H0R1), Homo sapiens (Q5TAX3), Homo sapiens (Q5VYS8)
Manually annotated by BRENDA team
Kim, H.; Kim, J.; Yu, S.; Lee, Y.Y.; Park, J.; Choi, R.J.; Yoon, S.J.; Kang, S.G.; Kim, V.N.
A mechanism for microRNA arm switching regulated by uridylation
Mol. Cell
78
1224-1236
2020
Homo sapiens (Q5TAX3), Homo sapiens (Q5VYS8)
Manually annotated by BRENDA team
Yamashita, S.; Nagaike, T.; Tomita, K.
Crystal structure of the Lin28-interacting module of human terminal uridylyltransferase that regulates let-7 expression
Nat. Commun.
10
1960
2019
Homo sapiens (Q5TAX3), Homo sapiens
Manually annotated by BRENDA team
Yamashita, S.; Takagi, Y.; Nagaike, T.; Tomita, K.
Crystal structures of U6 snRNA-specific terminal uridylyltransferase
Nat. Commun.
8
15788
2017
Homo sapiens (F5H0R1), Homo sapiens
Manually annotated by BRENDA team
Xu, K.; Lin, J.; Zandi, R.; Roth, J.A.; Ji, L.
MicroRNA-mediated target mRNA cleavage and 3-uridylation in human cells
Sci. Rep.
6
30242
2016
Homo sapiens
Manually annotated by BRENDA team